http://lod.bco-dmo.org/id/dataset/776293
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-09-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Olympia oyster mortality samples from cultures in 50 unique combinations of temperature, salinity, pCO2 at Shannon Point Marine Center in May 2018
2019-09-05
publication
2019-09-05
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-31
publication
https://doi.org/10.26008/1912/bco-dmo.776293.1
Shawn M. Arellano
Western Washington University
principalInvestigator
M. Brady Olson
Western Washington University
principalInvestigator
Sylvia Yang
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Arellano, S., Olson, B., Yang, S. (2020) Olympia oyster mortality samples from cultures in 50 unique combinations of temperature, salinity, pCO2 at Shannon Point Marine Center in May 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-09-05 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.776293.1 [access date]
Olympia oysters Growth_Expt_2018_Mortality Dataset Description: <p>Olympia oyster mortality samples from cultures in 50 unique combinations of temperature, salinity, pCO2 at Shannon Point Marine Center in May 2018.</p> Methods and Sampling: <p>We cultured Olympia oyster larvae in 50 unique combinations of temperature, salinity, and pCO2. Larvae were collected on the day of release from Puget Sound Restoration Fund, and overnight shipped to Shannon Point Marine Center, rehydrated, and dispensed into treatment cups. Culture cups were housed in a custom-build head gradient flow-through water bath ranging from ambient (~12°C) to ~30°C and assigned a random salinity value (9-36, by threes). Also available is the dataset&nbsp;of cup chemistry.</p>
<p>To measure mortality in culture cups, we sampled larvae prior to water changes every two-three days of the experiment (Mon/Wed/Fri). Samplers condensed larval cups into small bowls, mixed larvae inside to suspend dead and alive larvae, and took 0.5-1ml aliquots on Sedgewick rafter counting cells. Our aim was to capture at least 20 live larvae in each sample. If fewer than 20 live larvae were sampled, the counter would take another aliquot and add numbers. If more, all larvae in aliquot we’re counted. Live and dead larvae were counted, and the live ones were collected for later size measurement (see growth experiment datasheet). The developmental stage was noted by the visual presence of eyespots on larval shells (though these were recounted in size samples later to improve consistency, so eye counts from this data sheet were not used), and presence of settled larvae on culture cup surfaces.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538626 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538626
completed
Shawn M. Arellano
Western Washington University
360-650-3634
Western Washington University 1900 Shannon Point Road
Anacortes
WA
98221
USA
arellas@wwu.edu
pointOfContact
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Sylvia Yang
Western Washington University
360-598-4460
SEA Discovery Center P.O. Box 2318
Poulsbo
WA
98370
Sylvia.yang@wwu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cup
Date
Cup_Date
Alive
Dead
Eyed
Counter
Mortality
Percent_eyed
Settlers
SettlersCount
Notes
Leica Stereoscope
theme
None, User defined
treatment
date
replicate
count
investigator
relative abundance
No BCO-DMO term
comments
featureType
BCO-DMO Standard Parameters
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
https://osprey.bco-dmo.org/project/684167
RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
<p>In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences</p>
<p>As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.</p>
Climate stressors on larvae
largerWorkCitation
project
eng; USA
biota
oceans
2018-05-04
2018-05-21
Coastal Pacific, USA
0
BCO-DMO catalogue of parameters from Olympia oyster mortality samples from cultures in 50 unique combinations of temperature, salinity, pCO2 at Shannon Point Marine Center in May 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/776313.rdf
Name: Cup
Units: unitless
Description: <p>Treatment cup representing a unique temperature/salinity/pCO2 combination</p>
http://lod.bco-dmo.org/id/dataset-parameter/776314.rdf
Name: Date
Units: unitless
Description: <p>Sample date from May 4 - May 21 2018; formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/776315.rdf
Name: Cup_Date
Units: unitless
Description: <p>Concatenated cup label and date; formatted as Label_mmddyy</p>
http://lod.bco-dmo.org/id/dataset-parameter/776316.rdf
Name: Alive
Units: Larvae
Description: <p>Number of live larvae in sample aliquot</p>
http://lod.bco-dmo.org/id/dataset-parameter/776317.rdf
Name: Dead
Units: Larvae
Description: <p>Number of dead larvae in sample aliquot</p>
http://lod.bco-dmo.org/id/dataset-parameter/776318.rdf
Name: Eyed
Units: Larvae
Description: <p>Number of larvae with visible eye spots. Eyes were later recounted in photographic size sampling (Growth dataet)</p>
http://lod.bco-dmo.org/id/dataset-parameter/776319.rdf
Name: Counter
Units: Initials
Description: <p>Initials of sampler</p>
http://lod.bco-dmo.org/id/dataset-parameter/776320.rdf
Name: Mortality
Units: unitless
Description: <p>Percent mortality (total dead / total sampled)</p>
http://lod.bco-dmo.org/id/dataset-parameter/776321.rdf
Name: Percent_eyed
Units: unitless
Description: <p>Percent eyed (total eyed / total sampled)</p>
http://lod.bco-dmo.org/id/dataset-parameter/776322.rdf
Name: Settlers
Units: unitless
Description: <p>Presence of settled larvae in culture cup: yes/no</p>
http://lod.bco-dmo.org/id/dataset-parameter/776323.rdf
Name: SettlersCount
Units: Individuals
Description: <p>Number of settlers in culture cup</p>
http://lod.bco-dmo.org/id/dataset-parameter/776324.rdf
Name: Notes
Units: unitless
Description: <p>Notes on physical data sheet</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
15821
https://darchive.mblwhoilibrary.org/bitstream/1912/26140/1/dataset-776293_olygrowthexpt2018mortality__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.776293.1
download
onLine
dataset
<p>We cultured Olympia oyster larvae in 50 unique combinations of temperature, salinity, and pCO2. Larvae were collected on the day of release from Puget Sound Restoration Fund, and overnight shipped to Shannon Point Marine Center, rehydrated, and dispensed into treatment cups. Culture cups were housed in a custom-build head gradient flow-through water bath ranging from ambient (~12°C) to ~30°C and assigned a random salinity value (9-36, by threes). Also available is the dataset&nbsp;of cup chemistry.</p>
<p>To measure mortality in culture cups, we sampled larvae prior to water changes every two-three days of the experiment (Mon/Wed/Fri). Samplers condensed larval cups into small bowls, mixed larvae inside to suspend dead and alive larvae, and took 0.5-1ml aliquots on Sedgewick rafter counting cells. Our aim was to capture at least 20 live larvae in each sample. If fewer than 20 live larvae were sampled, the counter would take another aliquot and add numbers. If more, all larvae in aliquot we’re counted. Live and dead larvae were counted, and the live ones were collected for later size measurement (see growth experiment datasheet). The developmental stage was noted by the visual presence of eyespots on larval shells (though these were recounted in size samples later to improve consistency, so eye counts from this data sheet were not used), and presence of settled larvae on culture cup surfaces.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- re-formatted date from mdyy to yyyy-mm-dd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Leica Stereoscope
Leica Stereoscope
PI Supplied Instrument Name: Leica Stereoscope Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/