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        <gco:CharacterString>Carbonate chemistry data from static and fluctuating pCO2 x dissolved oxygen (DO) experiments on Menidia menidia Dataset Description: &amp;lt;p&amp;gt;Carbonate chemistry data from static and fluctuating pCO2 x dissolved oxygen (DO) experiments on Menidia menidia. Four separate experiments were conducted over two consecutive years to determine the effects of static and fluctuating pCO2 x DO conditions on the early life survival and growth of the coastal forage fish, M. menidia.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;See related datasets: &amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/777117&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;survival&amp;lt;/a&amp;gt; and &amp;lt;a href=&amp;quot;https://www.bco-dmo.org/dataset/777130&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;growth&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Wild adults were collected using a 30 x 2 m beach seine and strip-spawned in the laboratory the following day. 100 embryos were then placed in each replicate across 9 recirculating systems of different pCO2 x DO conditions (control, intermediate, extreme) and cycling patterns (static, small diel fluctuation, large diel fluctuation and tidal fluctuation).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;pCO2 x DO conditions were measured every hour for each tank and adjusted to the pre-determined conditions via the injection of carbon dioxide, nitrogen gas and/or CO2-stripped air.&amp;amp;nbsp;LabView software (National Instruments) was used to control sampling pumps and gas and water solenoids.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Seawater samples of each tank were collected three times throughout each experiment and stored in 4°C for subsequent alkalinity measurements up to two weeks after the experiment finished. For more details please see Cross et al. (submitted).&amp;lt;/p&amp;gt;</gco:CharacterString>
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Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the &quot;Women in Science and Engineering&quot; program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations.&lt;/p&gt;
&lt;p&gt;This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 × DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 × 3 DO levels. Second, the effects of tidal and diel CO2 × DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature × CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Related references:&lt;/strong&gt;&lt;br /&gt;
Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:&lt;a href=&quot;http://dx.doi.org/10.1098/rsbl.2015.0976&quot; target=&quot;_blank&quot;&gt;10.1098/rsbl.2015.0976&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:&lt;a href=&quot;http://dx.doi.org/10.1002/loe2.10002&quot; target=&quot;_blank&quot;&gt;10.1002/loe2.10002&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:&lt;a href=&quot;http://dx.doi.org/10.3354/meps11142&quot; target=&quot;_blank&quot;&gt;10.3354/meps11142&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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