http://lod.bco-dmo.org/id/dataset/778653
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-10-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chlorophyll data from daily sampling at the Santa Monica Pier, CA from April to May of 2019
2019-10-03
publication
2019-10-03
revision
BCO-DMO Linked Data URI
2019-10-03
creation
http://lod.bco-dmo.org/id/dataset/778653
David Caron
University of Southern California
principalInvestigator
Jennifer Beatty
University of Southern California
principalInvestigator
Julie V. Hopper
University of Southern California
principalInvestigator
Gerid A. Ollison
University of Southern California
principalInvestigator
Brittany P. Stewart
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Caron, D., Beatty, J., Hopper, J. V., Ollison, G. A. (2019) Chlorophyll data from daily sampling at the Santa Monica Pier, CA from April to May of 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-10-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/778653 [access date]
Dataset Description: <p>Daily Chlorophylla (ug/L) data from surface waters off of the Santa Monica Pier, California from April 13th to May 5th 2019 as part of the San Pedro Ocean Time-series (SPOT).</p> Methods and Sampling: Chlorophyll Extraction by Non-Acidification Method
Whole seawater collected from Santa Monica Pier, CA by bucket off pier. Portion of whole seawater placed in dark for chlorophyll processing. Bottle shaken, then filtered on to GFF filter (Glass Microfiber Filter, 0.7um, 25mm,Whatman) using tower rig and gast pump vacuum. Triplicates taken for each day. Wrapped in foil and flash frozen in liquid nitrogen to preserve. Stored in lab in -80C freezer till processed.
In Lab processing: using glass cuvette (Disposable Culture Tubes, Borosilicate Glass, 12x75mm, VWR), place individual filter inside, add 4ml 100% Acetone, and cap. Ensure filter is submerged in acetone. Wrap all samples in foil and place in -20C freezer for 18-24hr. Between 18-24hrs remove samples and thaw for 30 minutes, keep in dark. Using Turner Design Fluorometer, calibrate using stored standard. For samples, invert sample multiple times, uncap, using metal spatula remove filter, use Kimwipe to clean off outside of cuvette, place in fluorometer and record both raw fluoresce and direct concentration. Dilution may be necessary if raw fluoresce is above 250 RFU.
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1737409 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1737409
onGoing
David Caron
University of Southern California
213-740-0203
Dornsife College of Letters, Arts, and Sciences 3616 Trousdale Parkway
Los Angeles
CA
90089
USA
dcaron@usc.edu
pointOfContact
Jennifer Beatty
University of Southern California
jlbeatty@usc.edu
pointOfContact
Julie V. Hopper
University of Southern California
juliehop@usc.edu
pointOfContact
Gerid A. Ollison
University of Southern California
gollison@usc.edu
pointOfContact
Brittany P. Stewart
University of Southern California
bs70959@usc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
SAMPLE
Chl_Concentration
Lat
Lon
Turner Design Instruments Model# 7200-000
theme
None, User defined
date
sample identification
chlorophyll a
latitude
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Turner Designs 700 Laboratory Fluorometer
instrument
BCO-DMO Standard Instruments
SPOT_SMP
service
Deployment Activity
Santa Monica Pier, CA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Protistan, prokaryotic, and viral processes at the San Pedro Ocean Time-series
https://osprey.bco-dmo.org/project/743049
Protistan, prokaryotic, and viral processes at the San Pedro Ocean Time-series
<p>Planktonic marine microbial communities consist of a diverse collection of bacteria, archaea, viruses, protists (phytoplankton and protozoa) and small animals (metazoan). Collectively, these species are responsible for virtually all marine pelagic primary production where they form the basis of food webs and carry out a large fraction of respiratory processes. Microbial interactions include the traditional role of predation, but recent research recognizes the importance of parasitism, symbiosis and viral infection. Characterizing the response of pelagic microbial communities and processes to environmental influences is fundamental to understanding and modeling carbon flow and energy utilization in the ocean, but very few studies have attempted to study all of these assemblages in the same study. This project is comprised of long-term (monthly) and short-term (daily) sampling at the San Pedro Ocean Time-series (SPOT) site. Analysis of the resulting datasets investigates co-occurrence patterns of microbial taxa (e.g. protist-virus and protist-prokaryote interactions, both positive and negative) indicating which species consistently co-occur and potentially interact, followed by examination gene expression to help define the underlying mechanisms. This study augments 20 years of baseline studies of microbial abundance, diversity, rates at the site, and will enable detection of low-frequency changes in composition and potential ecological interactions among microbes, and their responses to changing environmental forcing factors. These responses have important consequences for higher trophic levels and ocean-atmosphere feedbacks. The broader impacts of this project include training graduate and undergraduate students, providing local high school student with summer lab experiences, and PI presentations at local K-12 schools, museums, aquaria and informal learning centers in the region. Additionally, the PIs advise at the local, county and state level regarding coastal marine water quality.</p>
<p>This research project is unique in that it is a holistic study (including all microbes from viruses to small metazoa) of microbial species diversity and ecological activities, carried out at the SPOT site off the coast of southern California. In studying all microbes simultaneously, this work aims to identify important ecological interactions among microbial species, and identify the basis(es) for those interactions. This research involves (1) extensive analyses of prokaryote (archaean and bacterial) and eukaryote (protistan and micro-metazoan) diversity via the sequencing of marker genes, (2) studies of whole-community gene expression by eukaryotes and prokaryotes in order to identify key functional characteristics of microorganismal groups and the detection of active viral infections, and (3) metagenomic analysis of viruses and bacteria to aid interpretation of transcriptomic analyses using genome-encoded information. The project includes exploratory metatranscriptomic analysis of poorly-understood aphotic and hypoxic-zone protists, to examine their stratification, functions and hypothesized prokaryotic symbioses.</p>
SPOT
largerWorkCitation
project
eng; USA
oceans
Santa Monica Pier, CA
-118.50015
-118.50015
34.00765
34.00765
2019-04-13
2019-05-05
San Pedro Channel off the coast of Los Angeles
0
BCO-DMO catalogue of parameters from Chlorophyll data from daily sampling at the Santa Monica Pier, CA from April to May of 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/778671.rdf
Name: Date
Units: unitless
Description: <p>Date (local,UTC-7) in format m/d/yyyy</p>
http://lod.bco-dmo.org/id/dataset-parameter/778672.rdf
Name: SAMPLE
Units: unitless
Description: <p>Sample name</p>
http://lod.bco-dmo.org/id/dataset-parameter/778673.rdf
Name: Chl_Concentration
Units: micrograms per liter (µg/l)
Description: <p>Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/778674.rdf
Name: Lat
Units: decimal degrees
Description: <p>Latitude (south is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/778675.rdf
Name: Lon
Units: nd
Description: <p>Longitude (west is negative)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2965
https://datadocs.bco-dmo.org/file/zqqADBVswlGgoE/chl.csv
chl.csv
Primary data file for dataset ID 778653
download
https://osprey.bco-dmo.org/dataset/778653/data/download
download
onLine
dataset
Chlorophyll Extraction by Non-Acidification Method
Whole seawater collected from Santa Monica Pier, CA by bucket off pier. Portion of whole seawater placed in dark for chlorophyll processing. Bottle shaken, then filtered on to GFF filter (Glass Microfiber Filter, 0.7um, 25mm,Whatman) using tower rig and gast pump vacuum. Triplicates taken for each day. Wrapped in foil and flash frozen in liquid nitrogen to preserve. Stored in lab in -80C freezer till processed.
In Lab processing: using glass cuvette (Disposable Culture Tubes, Borosilicate Glass, 12x75mm, VWR), place individual filter inside, add 4ml 100% Acetone, and cap. Ensure filter is submerged in acetone. Wrap all samples in foil and place in -20C freezer for 18-24hr. Between 18-24hrs remove samples and thaw for 30 minutes, keep in dark. Using Turner Design Fluorometer, calibrate using stored standard. For samples, invert sample multiple times, uncap, using metal spatula remove filter, use Kimwipe to clean off outside of cuvette, place in fluorometer and record both raw fluoresce and direct concentration. Dilution may be necessary if raw fluoresce is above 250 RFU.
Specified by the Principal Investigator(s)
<p>Chlorophyll data was processed in Microsoft Word Excel 2011, for calibration correction on samples and averaging triplicate values.</p>
<p>BCO-DMO data manager processing notes:<br />
* column names modified to remove spaces, units, and parentheses.&nbsp; Column names, descriptions and units can be found in the "Parameter Description" section.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner Design Instruments Model# 7200-000
Turner Design Instruments Model# 7200-000
PI Supplied Instrument Name: Turner Design Instruments Model# 7200-000 Instrument Name: Turner Designs 700 Laboratory Fluorometer Instrument Short Name:TD-700 Instrument Description: The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/
Deployment: SPOT_SMP
SPOT_SMP
shoreside Santa Monica Pier
dock
SPOT_SMP
David Caron
University of Southern California
shoreside Santa Monica Pier
dock