http://lod.bco-dmo.org/id/dataset/778881
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-10-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Algal pigment concentrations measured by HPLC from RVIB Nathaniel B. Palmer cruise in the Ross Sea, Southern Ocean from 2017-2018.
2019-12-24
publication
2019-12-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-10-21
publication
https://doi.org/10.1575/1912/bco-dmo.778881.1
Giacomo DiTullio
College of Charleston
principalInvestigator
Peter Lee
College of Charleston
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: DiTullio, G., Lee, P. (2019) Algal pigment concentrations measured by HPLC from RVIB Nathaniel B. Palmer cruise in the Ross Sea, Southern Ocean from 2017-2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2019-12-24 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.778881.2 [access date]
Dataset Description: <p>Algal pigment concentrations as measured by HPLC from RVIB Nathaniel B. Palmer cruise in the Ross Sea, Southern Ocean from 2017-2018.</p> Methods and Sampling: <p>Algal HPLC samples were collected by gentile filtration under low vacuum through GF/F filters and frozen at -80C for on-shore analysis. Samples were extracted in acetone and analyzed using an Agilent 1100 HPLC system equipped with autosampler, photodiode array and fluorescence detectors. The gradient elution program utilized was a slight modification of the Zapata et al. method (2000). Complete details of the HPLC method are described elsewhere (DiTullio and Geesey 2002).</p>
<p>High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array and fluorescence detectors</p>
<p>&nbsp;</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1644073 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1644073
completed
Giacomo DiTullio
College of Charleston
843-953-9196
Hollings Marine Lab 205 Fort Johnson Rd.
Charleston
SC
29412
USA
ditullioj@cofc.edu
pointOfContact
Peter Lee
College of Charleston
843-460-9785
331 Fort Johnson Rd.
Charleston
SC
29412
USA
leep@cofc.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
Date
Station
Latitude
Longitude
Depth
Niskin
Sample
Fltr_Vol
Chl_C3
Chl_lide
MgDVP
Chl_C2
Chl_C1
Peridinin
Ph_ide
But_19
Fuco
Neo
Prasino
Viola
Hex_19
Diadino
cis_fuco
Allo
Diato
Monad
Zea
Lutein
Croco
Chl_b
Chl_a_allomer
Chl_C2_MGDG
DV_Chl_a
Chl_a
Ph_tin
a_Car
b_Car
Agilent 1100
theme
None, User defined
date
station
latitude
longitude
depth
Niskin bottle number
sample identification
volume of water filtered
chlorophyll c3
chlorophyllide
No BCO-DMO term
chlorophyll c2
chlorophyll c1
peridinin
pheophorbide a
19-prime-butanoyloxyfucoxanthin
fucoxanthin
neoxanthin
prasinoxanthin
violaxanthin
19-prime-hexanoyloxyfucoxanthin
diadinoxanthin
alloxanthin
diatoxanthin
monad
zeaxanthin
lutein
crocoxanthin pigment
chlorophyll b
allomerized chlorophyll a
divinyl chlorophyll a
chlorophyll a
pheophytin a
carotene-alpha
carotene-beta
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
instrument
BCO-DMO Standard Instruments
NBP1801
service
Deployment Activity
Western Ross Sea, Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Cobalamin and Iron Co-Limitation Of Phytoplankton Species in Terra Nova Bay
https://osprey.bco-dmo.org/project/774945
Collaborative Research: Cobalamin and Iron Co-Limitation Of Phytoplankton Species in Terra Nova Bay
<p>NSF abstract:<br />
Phytoplankton blooms in the coastal waters of the Ross Sea, Antarctica are typically dominated by either diatoms or Phaeocystis Antarctica (a flagellated algae that often can form large colonies in a gelatinous matrix). The project seeks to determine if an association of bacterial populations with Phaeocystis antarctica colonies can directly supply Phaeocystis with Vitamin B12, which can be an important co-limiting micronutrient in the Ross Sea. The supply of an essential vitamin coupled with the ability to grow at lower iron concentrations may put Phaeocystis at a competitive advantage over diatoms. Because Phaeocystis cells can fix more carbon than diatoms and Phaeocystis are not grazed as efficiently as diatoms, the project will help in refining understanding of carbon dynamics in the region as well as the basis of the food web webs. Such understanding also has the potential to help refine predictive ecological models for the region. The project will conduct public outreach activities and will contribute to undergraduate and graduate research. Engagement of underrepresented students will occur during summer student internships. A collaboration with Italian Antarctic researchers, who have been studying the Terra Nova Bay ecosystem since the 1980s, aims to enhance the project and promote international scientific collaborations.</p>
<p>The study will test whether a mutualistic symbioses between attached bacteria and Phaeocystis provides colonial cells a mechanism for alleviating chronic Vitamin B12 co-limitation effects thereby conferring them with a competitive advantage over diatom communities. The use of drifters in a time series study will provide the opportunity to track in both space and time a developing algal bloom in Terra Nova Bay and to determine community structure and the physiological nutrient status of microbial populations. A combination of flow cytometry, proteomics, metatranscriptomics, radioisotopic and stable isotopic labeling experiments will determine carbon and nutrient uptake rates and the role of bacteria in mitigating potential vitamin B12 and iron limitation. Membrane inlet and proton transfer reaction mass spectrometry will also be used to estimate net community production and release of volatile organic carbon compounds that are climatically active. Understanding how environmental parameters can influence microbial community dynamics in Antarctic coastal waters will advance an understanding of how changes in ocean stratification and chemistry could impact the biogeochemistry and food web dynamics of Southern Ocean ecosystems.</p>
CICLOPS
largerWorkCitation
project
eng; USA
oceans
Western Ross Sea, Southern Ocean
167.5528
-116.9882
-78.6294
-72.44818
2017-12-31
2018-02-19
Amundsen Sea, Ross Sea, Terra Nova Bay
0
BCO-DMO catalogue of parameters from Algal pigment concentrations measured by HPLC from RVIB Nathaniel B. Palmer cruise in the Ross Sea, Southern Ocean from 2017-2018.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/778985.rdf
Name: Date
Units: unitless
Description: <p>Date (UTC) - format: yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/778986.rdf
Name: Station
Units: unitless
Description: <p>Station Identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/778987.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude (South is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/778988.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude (West is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/778989.rdf
Name: Depth
Units: meters (m)
Description: <p>Sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/778990.rdf
Name: Niskin
Units: unitless
Description: <p>Niskin Bottle Number</p>
http://lod.bco-dmo.org/id/dataset-parameter/778991.rdf
Name: Sample
Units: unitless
Description: <p>Sample Number</p>
http://lod.bco-dmo.org/id/dataset-parameter/778992.rdf
Name: Fltr_Vol
Units: liter (L)
Description: <p>Volume Filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/778993.rdf
Name: Chl_C3
Units: nanogram per liter (ng/L)
Description: <p>Chlorophyll c3</p>
http://lod.bco-dmo.org/id/dataset-parameter/778994.rdf
Name: Chl_lide
Units: nanogram per liter (ng/L)
Description: <p>Chlorophyllide</p>
http://lod.bco-dmo.org/id/dataset-parameter/778995.rdf
Name: MgDVP
Units: nanogram per liter (ng/L)
Description: <p>Magnesium-2;4-divinyl</p>
http://lod.bco-dmo.org/id/dataset-parameter/778996.rdf
Name: Chl_C2
Units: nanogram per liter (ng/L)
Description: <p>Chlorophyll c2</p>
http://lod.bco-dmo.org/id/dataset-parameter/778997.rdf
Name: Chl_C1
Units: nanogram per liter (ng/L)
Description: <p>Chlorophyll c1</p>
http://lod.bco-dmo.org/id/dataset-parameter/778998.rdf
Name: Peridinin
Units: nanogram per liter (ng/L)
Description: <p>Peridinin</p>
http://lod.bco-dmo.org/id/dataset-parameter/778999.rdf
Name: Ph_ide
Units: nanogram per liter (ng/L)
Description: <p>Pheophorbide a</p>
http://lod.bco-dmo.org/id/dataset-parameter/779000.rdf
Name: But_19
Units: nanogram per liter (ng/L)
Description: <p>19'-butanoyloxyfucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779001.rdf
Name: Fuco
Units: nanogram per liter (ng/L)
Description: <p>Fucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779002.rdf
Name: Neo
Units: nanogram per liter (ng/L)
Description: <p>Neoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779003.rdf
Name: Prasino
Units: nanogram per liter (ng/L)
Description: <p>Prasinoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779004.rdf
Name: Viola
Units: nanogram per liter (ng/L)
Description: <p>Violaxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779005.rdf
Name: Hex_19
Units: nanogram per liter (ng/L)
Description: <p>19'-hexanoyloxyfucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779006.rdf
Name: Diadino
Units: nanogram per liter (ng/L)
Description: <p>Diadinoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779007.rdf
Name: cis_fuco
Units: nanogram per liter (ng/L)
Description: <p>cis-Fucoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779008.rdf
Name: Allo
Units: nanogram per liter (ng/L)
Description: <p>Alloxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779009.rdf
Name: Diato
Units: nanogram per liter (ng/L)
Description: <p>Diatoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779010.rdf
Name: Monad
Units: nanogram per liter (ng/L)
Description: <p>Monadoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779011.rdf
Name: Zea
Units: nanogram per liter (ng/L)
Description: <p>Zeaxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779012.rdf
Name: Lutein
Units: nanogram per liter (ng/L)
Description: <p>Lutein</p>
http://lod.bco-dmo.org/id/dataset-parameter/779013.rdf
Name: Croco
Units: nanogram per liter (ng/L)
Description: <p>Crocoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/779014.rdf
Name: Chl_b
Units: nanogram per liter (ng/L)
Description: <p>Chlorophyll b</p>
http://lod.bco-dmo.org/id/dataset-parameter/779015.rdf
Name: Chl_a_allomer
Units: nanogram per liter (ng/L)
Description: <p>Chlorophyll a allomer</p>
http://lod.bco-dmo.org/id/dataset-parameter/779016.rdf
Name: Chl_C2_MGDG
Units: nanogram per liter (ng/L)
Description: <p>Chlorophyll c2 MGDG</p>
http://lod.bco-dmo.org/id/dataset-parameter/779017.rdf
Name: DV_Chl_a
Units: nanogram per liter (ng/L)
Description: <p>Divinyl chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/779018.rdf
Name: Chl_a
Units: nanogram per liter (ng/L)
Description: <p>Chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/779019.rdf
Name: Ph_tin
Units: nanogram per liter (ng/L)
Description: <p>Phaeophytin a</p>
http://lod.bco-dmo.org/id/dataset-parameter/779020.rdf
Name: a_Car
Units: nanogram per liter (ng/L)
Description: <p>Alpha-carotene</p>
http://lod.bco-dmo.org/id/dataset-parameter/779021.rdf
Name: b_Car
Units: nanogram per liter (ng/L)
Description: <p>Beta-carotene</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
55704
https://darchive.mblwhoilibrary.org/bitstream/1912/25123/1/dataset-778881_pigments-hplc__v2.tsv
download
55443
https://darchive.mblwhoilibrary.org/bitstream/1912/24706/1/dataset-778881_pigments-hplc__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.778881.1
download
onLine
dataset
<p>Algal HPLC samples were collected by gentile filtration under low vacuum through GF/F filters and frozen at -80C for on-shore analysis. Samples were extracted in acetone and analyzed using an Agilent 1100 HPLC system equipped with autosampler, photodiode array and fluorescence detectors. The gradient elution program utilized was a slight modification of the Zapata et al. method (2000). Complete details of the HPLC method are described elsewhere (DiTullio and Geesey 2002).</p>
<p>High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array and fluorescence detectors</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Pigment concentrations were determined using standard peak integration procedures with Agilent’s ChemStation (version B.03.02), and entered into Microsoft Excel Spreadsheets for submission to BCO-DMO. Parameters reported were: chlorophyll c3, chlorophyllide, magnesium-2,4-divinyl phaeoporphyrin a5 monomethyl ester, chlorophyll c2, chlorophyll c1, peridinin, pheophorbide a, 19-prime butanoyloxyfucoxanthin, fucoxanthin, neoxanthin, prasinoxanthin, violaxanthin, 19-prime hexanoyloxyfucoxanthin, diadinoxanthin, cis-fucoxanthin, alloxanthin, diatoxanthin, monadoxanthin, zeaxanthin, lutein, crocoxanthin, chlorophyll b, divinyl chlorophyll a, chlorophyll a, pheophytin a, carotene-alpha and carotene-beta.</p>
<p>BCO-DMO processing notes:</p>
<ul>
<li>Adjusted column names</li>
<li>Adjusted date format to yyyy-mm-dd for increased interoperability</li>
<li>Version 2: An updated calibration curve was used to recalculate the chlorophyll a concentrations</li>
</ul>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Agilent 1100
Agilent 1100
PI Supplied Instrument Name: Agilent 1100 PI Supplied Instrument Description:High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array and fluorescence detectors.
Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Cruise: NBP1801
NBP1801
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
NBP1801
Giacomo DiTullio
College of Charleston
https://service.rvdata.us/data/cruise/NBP1801/doc/NBP1801DATA.pdf
Report describing NBP1801
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel