http://lod.bco-dmo.org/id/dataset/779387
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-10-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Experiment details for invertebrate larvae electrophysiology
2019-10-23
publication
2019-10-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-11-01
publication
https://doi.org/10.1575/1912/bco-dmo.779387.1
Lisa A. Levin
University of California-San Diego
principalInvestigator
Nicholas Oesch
University of California-San Diego
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Levin, L., Oesch, N. (2019) Experiment details for invertebrate larvae electrophysiology. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-10-23 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.779387.1 [access date]
Dataset Description: <p>Details on the timescale, pH, and temperature of the time series experiments from McCormick et al., 2019</p>
<p>Please see additional datasets for this paper, including the “TimeSeries” and the “OxygenMetrics_Vision” datasets.</p> Methods and Sampling: <p>Detailed methods can be found in McCormick, <em>et al., </em>2019.</p>
<p>Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m<strong>−</strong>2 s<strong>−</strong>1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution. This dataset shows additional experimental details for the “TimeSeries” and “OxygenMetrics_Vision” datasets, including the original experiment name (that can be matched with the experiment name from the other datasets), the time periods for each oxygen exposure, and the pH and temperature of the experiments.</p>
<p>Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.</p>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829623 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829623
completed
Lisa A. Levin
University of California-San Diego
858-534-3579
9500 Gilman Drive Mail Code: 0218
La Jolla
CA
92093-0218
USA
llevin@ucsd.edu
pointOfContact
Nicholas Oesch
University of California-San Diego
858-534-2991
9500 Gilman Dr.
La Jolla
CA
92093-0109
USA
noesch@mail.ucsd.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Experiment_Name
Normoxia_Time
O2_Decay
Normoxia_O2
Low_O2
O2_Change
O2_Change_Time
Low_O2_Min
Total_Exp_Time
pH
Temp_Max
Temp_Min
Temp_Avg
Stimulus_Irradiance
theme
None, User defined
No BCO-DMO term
experiment id
dissolved Oxygen
incubation time or duration
dissolved Oxygen
duration
pH
temperature of sample or a generic measurement (not in-situ water temp)
irradiance
featureType
BCO-DMO Standard Parameters
Oxygen Microelectrode Sensor
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
https://osprey.bco-dmo.org/project/775843
Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
<p>NSF abstract:<br />
Oxygen is being lost in the ocean worldwide as a result of ocean warming and the input of nutrients from land. Vision requires a large amount of oxygen, and may be less effective or require more light when oxygen is in short supply. This is especially true for active marine animals with complex eyes and visual capabilities, including active arthropods (crabs), cephalopods (squid), and fish. The California coastal waters exhibit a sharp drop in oxygen and light with increasing water depth. This project examines how visual physiology and ecology in young (larval) highly visual marine animals respond to oxygen loss, with a focus on key fisheries and aquaculture species. Experiments and observations will test the hypothesis that oxygen stress will change the light required for these organisms to see effectively, influencing the water depths where they can live and survive. The project will provide interdisciplinary experiences to students and an early career scientist and inform both the public (through outreach at the Birch Aquarium at Scripps Institution of Oceanography) and policy makers about the effects of oxygen decline in the ocean.</p>
<p>Negative effects of oxygen loss on vision have been described for humans and other terrestrial organisms, but never in the marine environment, despite the large changes in oxygen that can occur with depth and over time in the ocean, and the high metabolic demand of visual systems. This project will test the effects of low oxygen on vision in 3 combinations of eye design and photo-transduction mechanisms: compound eye with rhabdomeric photoreceptors (arthropods), simple eye with rhabdomeric photoreceptors (cephalopods), and simple eye with ciliary photoreceptors (fish). A series of oxygen- and light-controlled laboratory experiments will be conducted on representative taxa of each group including the tuna crab, Pleuroncodes planipes; the market squid, Doryteuthis opalescens, and the white sea bass, Atractoscion nobilis. In vivo electrophysiology and behavioral phototaxis experiments will identify new oxygen metrics for visual physiology and function, and will be compared to metabolic thresholds determined in respiration experiments. Hydrographic data collected over 3 decades by the CalCOFI program in the Southern California Bight will be evaluated with respect to visual and metabolic limits to determine the consequences of oxygen variation on the critical luminoxyscape (range of oxygen and light conditions required for visual physiology and function in target species) boundary in each species. Findings for the three vision-based functional groups may test whether oxygen-limited visual responses offer an additional explanation for the shoaling of species distributions among highly visual pelagic taxa in low oxygen, and will help to focus future research efforts and better understand the stressors contributing to habitat compression with expanding oxygen loss in the ocean.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Vision under hypoxia
largerWorkCitation
project
eng; USA
oceans
2019-10-23
Southern California Bight, Northeast Pacific Ocean
0
BCO-DMO catalogue of parameters from Experiment details for invertebrate larvae electrophysiology
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/779726.rdf
Name: Species
Units: unitless
Description: <p>Species name</p>
http://lod.bco-dmo.org/id/dataset-parameter/779727.rdf
Name: Experiment_Name
Units: unitless
Description: <p>Original experiment name that can be linked back to raw datafile collected in Igor.</p>
http://lod.bco-dmo.org/id/dataset-parameter/779728.rdf
Name: Normoxia_Time
Units: minute (min)
Description: <p>Total time (in minutes) that was spend at 100-105% saturation oxygen before oxygen decline. NA indicates there were no visual recordings in normoxia.</p>
http://lod.bco-dmo.org/id/dataset-parameter/779729.rdf
Name: O2_Decay
Units: minute (min)
Description: <p>Total time during which the animal was exposed to a decrease in oxygen (in minutes)</p>
http://lod.bco-dmo.org/id/dataset-parameter/779730.rdf
Name: Normoxia_O2
Units: micromole per liter (umol/L)
Description: <p>The average oxygen concentration of the normoxia (~100-105% saturation) period</p>
http://lod.bco-dmo.org/id/dataset-parameter/779731.rdf
Name: Low_O2
Units: micromole per liter (umol/L)
Description: <p>The average oxygen concentration of the lowest oxygen period</p>
http://lod.bco-dmo.org/id/dataset-parameter/779732.rdf
Name: O2_Change
Units: micromole per liter (umol/L)
Description: <p>Subtracted value between the average in normoxia and the average in the low oxygen period</p>
http://lod.bco-dmo.org/id/dataset-parameter/779733.rdf
Name: O2_Change_Time
Units: micromole per liter per minute (umol/l per min)
Description: <p>The rate of decline in oxygen (see above) over the total time when the animal was exposed to a decline in oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/779734.rdf
Name: Low_O2_Min
Units: minute (min)
Description: <p>Total time (in minutes) the animal was exposed to Low oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/779735.rdf
Name: Total_Exp_Time
Units: hour (hr)
Description: <p>Total experiment duration in hours</p>
http://lod.bco-dmo.org/id/dataset-parameter/779736.rdf
Name: pH
Units: unitless
Description: <p>pH of the seawater during the experiment. No buffer indicates the solution was not buffered and the pH might have varied during the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/779737.rdf
Name: Temp_Max
Units: degrees Celsius (C
Description: <p>Maximum temperature of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/779738.rdf
Name: Temp_Min
Units: degrees Celsius (C
Description: <p>Minimum temperature of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/779739.rdf
Name: Temp_Avg
Units: degrees Celsius (C
Description: <p>Mean temperature of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/779740.rdf
Name: Stimulus_Irradiance
Units: mol photons per second and square meter (mol m-2s-1)
Description: <p>Irradiance of the light stimulus (LED 525 nm)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1882
https://darchive.mblwhoilibrary.org/bitstream/1912/24768/1/dataset-779387_experiment-metrics__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.779387.1
download
onLine
dataset
<p>Detailed methods can be found in McCormick, <em>et al., </em>2019.</p>
<p>Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m<strong>−</strong>2 s<strong>−</strong>1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution. This dataset shows additional experimental details for the “TimeSeries” and “OxygenMetrics_Vision” datasets, including the original experiment name (that can be matched with the experiment name from the other datasets), the time periods for each oxygen exposure, and the pH and temperature of the experiments.</p>
<p>Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
<p>All electrophysiology data was recorded and analyzed using Igor Pro 7 Software (Wavemetrics) using custom code. Oxygen data was analyzed using PreSens Measurement Studio 2. Post-processing analysis was completed in R Studio (version 3.3.3).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe. Instrument Name: Oxygen Microelectrode Sensor Instrument Short Name:O2 Microelectrode Instrument Description: Any microelectrode sensor that measures oxygen. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/351/