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Detailed methods can be found in McCormick, LR; Levin, LA; Oesch, NW. 2019. Vision is highly sensitive to oxygen availability in marine invertebrate larvae. Journal of Experimental Biology 222, jeb200899. doi:10.1242\/jeb.200899. Data shown in Figure 1, bottom panel.<\/p>\n
Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 \u03bcmol photons m\u2212<\/strong>2 s\u2212<\/strong>1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in \u201cnormoxia\u201d (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution. This dataset shows the three visual metrics that were calculated to quantify the effects of reduced pO2 on retinal function. 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