http://lod.bco-dmo.org/id/dataset/779540
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-10-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Retinal responses during exposure to decreasing oxygen partial pressure (pO2)
2019-10-23
publication
2019-10-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-11-01
publication
https://doi.org/10.1575/1912/bco-dmo.779540.1
Lisa A. Levin
University of California-San Diego
principalInvestigator
Nicholas Oesch
University of California-San Diego
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Levin, L., Oesch, N. (2019) Retinal responses during exposure to decreasing oxygen partial pressure (pO2). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-10-23 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.779540.1 [access date]
Dataset Description: <p>Retinal responses during exposure to decreasing oxygen partial pressure (pO2) in marine invertebrate larvae determined with electroretinograms.</p>
<p>Please see additional datasets for this paper, including the “OxygenMetrics_Vision” and the “Experiment_Metrics” datasets.</p> Methods and Sampling: <p>Detailed methods can be found in McCormick <em>et al., </em>2019</p>
<p>Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m<strong>−</strong>2 s<strong>−</strong>1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution.</p>
<p>This dataset shows the retinal responses measured every 20 s for all individuals used in Figure 1 (top panel) of McCormick et al., 2019. The retinal responses show a decline in retinal function during a decrease in oxygen availability.</p>
<p>Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829623 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829623
completed
Lisa A. Levin
University of California-San Diego
858-534-3579
9500 Gilman Drive Mail Code: 0218
La Jolla
CA
92093-0218
USA
llevin@ucsd.edu
pointOfContact
Nicholas Oesch
University of California-San Diego
858-534-2991
9500 Gilman Dr.
La Jolla
CA
92093-0109
USA
noesch@mail.ucsd.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Experiment_Name
Round
O2_umol_l
O2_ml_l
O2_umol_kg
O2_pO2
n_ctrl_area
RunningAvg
RunAvgLine
Condition
theme
None, User defined
species scientific name (binomial)
experiment id
time_elapsed
dissolved Oxygen
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Oxygen Microelectrode Sensor
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
https://osprey.bco-dmo.org/project/775843
Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
<p>NSF abstract:<br />
Oxygen is being lost in the ocean worldwide as a result of ocean warming and the input of nutrients from land. Vision requires a large amount of oxygen, and may be less effective or require more light when oxygen is in short supply. This is especially true for active marine animals with complex eyes and visual capabilities, including active arthropods (crabs), cephalopods (squid), and fish. The California coastal waters exhibit a sharp drop in oxygen and light with increasing water depth. This project examines how visual physiology and ecology in young (larval) highly visual marine animals respond to oxygen loss, with a focus on key fisheries and aquaculture species. Experiments and observations will test the hypothesis that oxygen stress will change the light required for these organisms to see effectively, influencing the water depths where they can live and survive. The project will provide interdisciplinary experiences to students and an early career scientist and inform both the public (through outreach at the Birch Aquarium at Scripps Institution of Oceanography) and policy makers about the effects of oxygen decline in the ocean.</p>
<p>Negative effects of oxygen loss on vision have been described for humans and other terrestrial organisms, but never in the marine environment, despite the large changes in oxygen that can occur with depth and over time in the ocean, and the high metabolic demand of visual systems. This project will test the effects of low oxygen on vision in 3 combinations of eye design and photo-transduction mechanisms: compound eye with rhabdomeric photoreceptors (arthropods), simple eye with rhabdomeric photoreceptors (cephalopods), and simple eye with ciliary photoreceptors (fish). A series of oxygen- and light-controlled laboratory experiments will be conducted on representative taxa of each group including the tuna crab, Pleuroncodes planipes; the market squid, Doryteuthis opalescens, and the white sea bass, Atractoscion nobilis. In vivo electrophysiology and behavioral phototaxis experiments will identify new oxygen metrics for visual physiology and function, and will be compared to metabolic thresholds determined in respiration experiments. Hydrographic data collected over 3 decades by the CalCOFI program in the Southern California Bight will be evaluated with respect to visual and metabolic limits to determine the consequences of oxygen variation on the critical luminoxyscape (range of oxygen and light conditions required for visual physiology and function in target species) boundary in each species. Findings for the three vision-based functional groups may test whether oxygen-limited visual responses offer an additional explanation for the shoaling of species distributions among highly visual pelagic taxa in low oxygen, and will help to focus future research efforts and better understand the stressors contributing to habitat compression with expanding oxygen loss in the ocean.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Vision under hypoxia
largerWorkCitation
project
eng; USA
oceans
2019-10-23
Southern California Bight, Northeast Pacific Ocean
0
BCO-DMO catalogue of parameters from Retinal responses during exposure to decreasing oxygen partial pressure (pO2)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/780438.rdf
Name: Species
Units: unitless
Description: <p>Species name. Format is "Species_genus".</p>
http://lod.bco-dmo.org/id/dataset-parameter/780439.rdf
Name: Experiment_Name
Units: unitless
Description: <p>Original experiment name that can be linked back to raw datafile collected in Igor.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780440.rdf
Name: Round
Units: unitless
Description: <p>The number of seconds since starting each individual experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780441.rdf
Name: O2_umol_l
Units: micromole per liter (umol/L)
Description: <p>Oxygen concentration in umol O2/L for each retinal measurement.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780442.rdf
Name: O2_ml_l
Units: milliliter per liter (ml/l)
Description: <p>Oxygen concentration in mL O2/L for each retinal measurement.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780443.rdf
Name: O2_umol_kg
Units: micromole per kg (umol/kg)
Description: <p>Oxygen concentration in umol O2/kg for each retinal measurement.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780444.rdf
Name: O2_pO2
Units: kiloPascal (kPa)
Description: <p>The partial pressure of oxygen of the given metric “n.ctrl.area”: The ERG response normalized to the average retinal response measured during exposure to normoxia.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780445.rdf
Name: n_ctrl_area
Units: unitless
Description: <p>The integration of the ERG wave at any given time divided by the average integrated ERG during control oxygen conditions. Normalization was done to compare between individuals and species.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780446.rdf
Name: RunningAvg
Units: unitless
Description: <p>The running average of retinal responses in the last minute, used to show smoothed responses over time.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780447.rdf
Name: RunAvgLine
Units: unitless
Description: <p>The line number for the running average, used to order measurements within an individual experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/780448.rdf
Name: Condition
Units: unitless
Description: <p>Oxygen exposure during the experiment, either "normoxia", “decrease”, or “Low”. First, animals were exposed to “normoxia” (~100-105% oxygen saturation), then a “Deacrease” in oxygen (slow decline of oxygen), until the “Low” oxygen was reached.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
190082
https://darchive.mblwhoilibrary.org/bitstream/1912/24767/1/dataset-779540_retinal-response__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.779540.1
download
onLine
dataset
<p>Detailed methods can be found in McCormick <em>et al., </em>2019</p>
<p>Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m<strong>−</strong>2 s<strong>−</strong>1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution.</p>
<p>This dataset shows the retinal responses measured every 20 s for all individuals used in Figure 1 (top panel) of McCormick et al., 2019. The retinal responses show a decline in retinal function during a decrease in oxygen availability.</p>
<p>Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.</p>
Specified by the Principal Investigator(s)
<p>All electrophysiology data was recorded and analyzed using Igor Pro 7 Software (Wavemetrics) using custom code. Oxygen data was analyzed using PreSens Measurement Studio 2. Post-processing analysis was completed in R Studio (version 3.3.3).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe. Instrument Name: Oxygen Microelectrode Sensor Instrument Short Name:O2 Microelectrode Instrument Description: Any microelectrode sensor that measures oxygen. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/351/