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            <gco:CharacterString>Cite this dataset as: Maas, A. E., Lawson, G., Tarrant, A. M. (2019) Shell quality of pteropods held for 1-14 days in three CO2 treatments with pteropods collected with a Reeve net during R/V Tioga cruises in the Gulf of Maine from 2014 to 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-11-05 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/780791 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dataset Description: &amp;lt;p&amp;gt;Shell quality of pteropods held for 1-14 days in three CO2 treatments with pteropods collected with a Reeve net during R/V Tioga cruises in the Gulf of Maine from 2014 to&amp;amp;nbsp;2015.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
These data were published in Maas et al. (2018) and Maas et al. (in review).&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Related Datasets:&amp;amp;nbsp;&amp;lt;br /&amp;gt;
* Pteropod abundance and distribution:&amp;amp;nbsp;https://www.bco-dmo.org/dataset/780874&amp;lt;br /&amp;gt;
* Pteropod respiration experiments:&amp;amp;nbsp;https://www.bco-dmo.org/dataset/780886&amp;lt;/p&amp;gt; Methods and Sampling: Methodology: 

Pteropod adult (&amp;gt;0.5 mm, with fully developed wings/parapodia) were captured for experiments using a Reeve net with 333-m mesh and a large cod end that was deployed at slow speeds, for a short duration (&amp;lt; 1 h) with the aim of gently sampling live, undamaged specimens on various cruises. Tows were conducted at multiple stations in the Gulf of Maine (C42 22.1’ - 42 0.0’ N and 69 42.6’ - 70 15.4’ W). Individuals for respiration experiments were returned via coolers to a walk-in cold-room facility at the Woods Hole Oceanographic Institution within 8 h of collection. The cold-room was set at a constant temperature of 8C for all experiments. During each experimental period, water had been collected from an offshore site concurrent to pteropod sampling and had been returned to the lab in advance for filtration (0.2-m) and thermal equilibration. It had been bubbled with ambient air, which ranged from a calculated 380 to 440 atm over the seasonal cycle, for ~12 h prior to the arrival of the pteropods. Upon arrival, pteropods were placed in 1-L beakers of water containing the filtered in situ water (15 ind L-1) for 8-12 h to allow for further temperature acclimatization and gut clearance. After this period, healthy looking individuals (actively swimming or with parapodia extended) were placed into glass respiration chambers (containing optode spots; OXFOIL: PyroScience, Aachen Germany) with 2-3 mL fresh 0.2-m filtered water. A control chamber, without a pteropod, was set up for every fourth chamber to determine background bacterial respiration. The oxygen concentration was measured non-invasively at the start of the experiment and 24 h later using a FireStingO2 optical oxygen meter (PyroScience, Aachen Germany). At the conclusion of the experiment, each organism was visually inspected to confirm survival, briefly rinsed with DI water, placed in a pre-weighed aluminum dish, and weighed on a Cahn microbalance (C-33; 1 g precision) for wet mass. They were then dried for 3-7 days in a drying oven at 60C and weighed again to obtain dry mass. Final oxygen consumption rates were calculated based on the change in oxygen between the final and initial oxygen measurements, corrected for the bacterial respiration from the control chambers (mol O2 h-1). 

Sampling and analytical procedures:

Temperature in the experiments was continually monitored using the Pyrosciences temperature logger that comes with the Firesting meter and are reported for only the ~24 duration of the respiration experiments.

Carbonate chemistry parameters are calculated as the average (over the course of the whole 14 day experiment) of measured pH, DIC and TA values using CO2sys. To monitor the carbonate chemistry during the experiments, subsamples of water (50 mL) from each carboy were taken every 2–3 days and measured spectrophotometrically for pH as described in White et al. (2013). To more fully characterize the carbonate chemistry of the experimental treatments, discrete samples for analysis of dissolved inorganic carbon (DIC) and total alkalinity (TA) were also collected from the pre-equilibrated water at weekly intervals, prior to its transfer to the carboys (day 0 and 7) and from the water in each carboy right before it was replaced during water transfers (day 7 and 14). This provided both the starting and ending DIC and TA of each batch of water for the first two weeks, and the starting conditions for week 3. DIC was measured using an Apollo SciTech DIC auto-analyzer, while TA was measured using an Apollo SciTech alkalinity auto-titrator, a Ross combination pH electrode, and a pH meter (ORION 3 Star) based on a modified Gran titration method (detailed in Wang et al., 2017). pH is reported on the seawater scale (pHsw). Salinity was measured with a Hannah refractometer.</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/55175.rdf" xlink:title="OCE-1316040" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316040 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1316040</gmx:Anchor>
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NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
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Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels &quot;Hang On&quot; Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;This project will involve a series of five short cruises in 2013 and 2014, during which a variety of hydrographic, chemical, and biological data and samples will be collected, as well as a number of laboratory experiments examining pteropod physiology and gene expression.&lt;/p&gt;
&lt;p&gt;From NSF proposal abstract:&lt;/p&gt;
&lt;p&gt;Dissolution of excess anthropogenic CO2 into the ocean is causing the  marine environment to decrease in pH. This &quot;ocean acidification&quot; is  predicted to threaten a broad variety of marine organisms, particularly  calcifying animals such as the thecosome (i.e., shelled) pteropods.  These pelagic gastropods form an aragonite shell, are prey for a number  of commercially important fish, and are significant contributors to  carbon biogeochemistry. Their ecosystem importance, abundance, and  sensitivity to dissolution position them as an important group for  investigating the impacts of acidification. Our understanding of the  effect of high CO2 on pteropods and the pelagic ecosystem, however, is  limited primarily to short-term studies of adult calcification and  respiration response in the polar ecosystems. There have been no  seasonal studies of sensitivity and our understanding of the effect of  CO2 on pteropod early life stages is limited. Limacina retroversa is a  particularly abundant thecosome pteropod in the North Atlantic, where it  is prey for a number of fisheries species and other top predators. This  species is also the most common pteropod in the Gulf of Maine (GoM)  where it is present year round. L. retroversa thus offers the prospect  of a useful model pteropod species, given both its ecological importance  and its abundance in readily accessible waters. The investigators will  conduct a series of short cruises to sample L. retroversa on a seasonal  basis from local waters of the GoM near Cape Cod. The carbonate  chemistry of the GoM fluctuates seasonally, providing the  opportunity to assess the response of wild caught pteropods to natural  changes in CO2. By characterizing the carbonate chemistry of the water  column and measuring the metabolic rate, shell quality, and gene  expression of pteropods throughout the year, the researchers will  achieve a time series of pteropod sensitivity to CO2. Subsequently,  using experimental manipulations the investigators will explore the  effect of seasonal acclimation on pteropod response to short- and  medium-term exposure to enhanced CO2. Pteropods frequently lay eggs in  captivity, and at WHOI there is institutional expertise in maintaining  these individuals in the laboratory. Building on these strengths, the  researchers will also study the effect of CO2 on embryonic and larval  development in L. retroversa. These earliest life-stages of marine  calcifiers are thought to be especially sensitive since initial shell  precipitation and the highly energetic processes of growth and  development are impeded by CO2 exposure. They will also document  mortality, shell production, abnormality, and developmental rate of  clutches of pteropod embryos exposed to increased CO2.&lt;/p&gt;
&lt;p&gt;Intellectual  Merit: Thecosome pteropods are an abundant group of calcifying  zooplankters that have been chronically understudied, particularly in  temperate regions. Due to its accessibility and ecological importance,  L. retroversa can be developed as a valuable model, interesting both as  the dominant pteropod in the commercially-important GoM region and also  an abundant pteropod in the temperate waters of the North Atlantic. The  goal of this research is to augment our knowledge of the distribution of  L. retroversa, to attain an understanding of their seasonal sensitivity  to natural variability in CO2, and to see how this exposure impacts  responses to both short- and medium-term CO2 exposure. Using powerful  transcriptomic technologies, the research will transform our  understanding of this group by investigating the molecular mechanisms of  response in L. retroversa to both seasonality and varying durations and  intensities of acidification, contextualized by ecosystem- and  organism-level metrics. Furthermore the study will examine the  effect of CO2 on the eggs of pteropods for the first time, providing  insight into their sensitivity to an acidifying environment.&lt;/p&gt;</gco:CharacterString>
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	Units: unitless
	Description: &lt;p&gt;Year for cruise of collection in format yyyy&lt;/p&gt; 
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http://lod.bco-dmo.org/id/dataset-parameter/780913.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/780914.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/780916.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/780917.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/780918.rdf
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Pteropod adult (&amp;gt;0.5 mm, with fully developed wings/parapodia) were captured for experiments using a Reeve net with 333-m mesh and a large cod end that was deployed at slow speeds, for a short duration (&amp;lt; 1 h) with the aim of gently sampling live, undamaged specimens on various cruises. Tows were conducted at multiple stations in the Gulf of Maine (C42 22.1’ - 42 0.0’ N and 69 42.6’ - 70 15.4’ W). Individuals for respiration experiments were returned via coolers to a walk-in cold-room facility at the Woods Hole Oceanographic Institution within 8 h of collection. The cold-room was set at a constant temperature of 8C for all experiments. During each experimental period, water had been collected from an offshore site concurrent to pteropod sampling and had been returned to the lab in advance for filtration (0.2-m) and thermal equilibration. It had been bubbled with ambient air, which ranged from a calculated 380 to 440 atm over the seasonal cycle, for ~12 h prior to the arrival of the pteropods. Upon arrival, pteropods were placed in 1-L beakers of water containing the filtered in situ water (15 ind L-1) for 8-12 h to allow for further temperature acclimatization and gut clearance. After this period, healthy looking individuals (actively swimming or with parapodia extended) were placed into glass respiration chambers (containing optode spots; OXFOIL: PyroScience, Aachen Germany) with 2-3 mL fresh 0.2-m filtered water. A control chamber, without a pteropod, was set up for every fourth chamber to determine background bacterial respiration. The oxygen concentration was measured non-invasively at the start of the experiment and 24 h later using a FireStingO2 optical oxygen meter (PyroScience, Aachen Germany). At the conclusion of the experiment, each organism was visually inspected to confirm survival, briefly rinsed with DI water, placed in a pre-weighed aluminum dish, and weighed on a Cahn microbalance (C-33; 1 g precision) for wet mass. They were then dried for 3-7 days in a drying oven at 60C and weighed again to obtain dry mass. Final oxygen consumption rates were calculated based on the change in oxygen between the final and initial oxygen measurements, corrected for the bacterial respiration from the control chambers (mol O2 h-1). 

Sampling and analytical procedures:

Temperature in the experiments was continually monitored using the Pyrosciences temperature logger that comes with the Firesting meter and are reported for only the ~24 duration of the respiration experiments.

Carbonate chemistry parameters are calculated as the average (over the course of the whole 14 day experiment) of measured pH, DIC and TA values using CO2sys. To monitor the carbonate chemistry during the experiments, subsamples of water (50 mL) from each carboy were taken every 2–3 days and measured spectrophotometrically for pH as described in White et al. (2013). To more fully characterize the carbonate chemistry of the experimental treatments, discrete samples for analysis of dissolved inorganic carbon (DIC) and total alkalinity (TA) were also collected from the pre-equilibrated water at weekly intervals, prior to its transfer to the carboys (day 0 and 7) and from the water in each carboy right before it was replaced during water transfers (day 7 and 14). This provided both the starting and ending DIC and TA of each batch of water for the first two weeks, and the starting conditions for week 3. DIC was measured using an Apollo SciTech DIC auto-analyzer, while TA was measured using an Apollo SciTech alkalinity auto-titrator, a Ross combination pH electrode, and a pH meter (ORION 3 Star) based on a modified Gran titration method (detailed in Wang et al., 2017). pH is reported on the seawater scale (pHsw). Salinity was measured with a Hannah refractometer.</gco:CharacterString>
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                <gco:CharacterString>CO2sys_v2.1

Matlab processing script &amp;quot;transparency_code.m&amp;quot; was used (see Supplemental Files section for full description and download access).

There are no temperature and salinity values for 2015 in this dataset.

BCO-DMO Data Manager Processing Notes:
* Excel file exported as csv
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values in this dataset are displayed as &amp;quot;nd&amp;quot; for &amp;quot;no data.&amp;quot;  nd is the default missing data identifier in the BCO-DMO system.</gco:CharacterString>
              </gmd:description>
              <gmd:source>
                <gmd:LI_Source>
                  <gmd:sourceCitation>
                    <gmd:CI_Citation>
                      <gmd:title>
                        <gco:CharacterString>Specified by the Principal Investigator(s)</gco:CharacterString>
                      </gmd:title>
                      <gmd:date gco:nilReason="unknown"/>
                    </gmd:CI_Citation>
                  </gmd:sourceCitation>
                </gmd:LI_Source>
              </gmd:source>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
   </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataMaintenance>
    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded" codeSpace="009">asNeeded</gmd:MD_MaintenanceFrequencyCode>
      </gmd:maintenanceAndUpdateFrequency>
      <gmd:maintenanceNote>
        <gco:CharacterString>7.x-1.1</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
				  </gmd:facsimile>
				</gmd:CI_Telephone>
		  </gmd:phone>
		  <gmd:address>
		    <gmd:CI_Address>
				  <gmd:deliveryPoint>
				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
				  </gmd:deliveryPoint>
				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
				  </gmd:postalCode>
				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
		</gmd:CI_Contact>
  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact"  codeSpace="007">pointOfContact</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation>
    <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/727.rdf" xlink:title="Optode" xlink:actuate="onRequest">OXFOIL: PyroScience, Aachen Germany</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>OXFOIL: PyroScience, Aachen Germany</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: OXFOIL: PyroScience, Aachen Germany Instrument Name: Optode Instrument Short Name:   Instrument Description: An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/705.rdf" xlink:title="Oxygen Sensor" xlink:actuate="onRequest">FireStingO2 optical oxygen meter</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>FireStingO2 optical oxygen meter</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: FireStingO2 optical oxygen meter PI Supplied Instrument Description:FireStingO2 optical oxygen meter (PyroScience, Aachen Germany) Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor   Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/674.rdf" xlink:title="pH Sensor" xlink:actuate="onRequest"></gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString></gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name:  PI Supplied Instrument Description:Ross combination pH electrode, and a pH meter (ORION 3 Star) based on a modified Gran titration method (detailed in Wang et al., 2017). Instrument Name: pH Sensor Instrument Short Name:pH Sensor   Instrument Description: An instrument that measures the hydrogen ion activity in solutions.

The overall concentration of hydrogen ions is inversely related to its pH.  The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+). </gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/443.rdf" xlink:title="Reeve Net" xlink:actuate="onRequest">Reeve net with 333-m mesh and a large cod end</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Reeve net with 333-m mesh and a large cod end</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Reeve net with 333-m mesh and a large cod end Instrument Name: Reeve Net Instrument Short Name:Reeve Net   Instrument Description: A Reeve Net is a conventional ring net with a very large acrylic cylindrical cod-end (30 liters) designed to collect fragile gelatinous animals. The net is lowered to a particular depth and then hauled slowly back to the surface (5-10 m/min). Reeve (1981) also described a double net system with no bridle and flotation at the net mouth that is attached to a roller mechanism that rides on a tow wire.
The roller system is locked in place by a pressure release device. Once below a set pressure, the roller
and nets are released and they float slowly up the wire, gently collecting the zooplankton, without being
influenced by the motion of the vessel and associated vertical wire movements. (from Wiebe and Benfield, 2003) Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/NETT0147/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/679.rdf" xlink:title="Refractometer" xlink:actuate="onRequest">Hannah refractometer</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Hannah refractometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Hannah refractometer Instrument Name: Refractometer Instrument Short Name:Refractometer   Instrument Description: A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from Snell's law and can be calculated from the composition of the material using the Gladstone-Dale relation.

In optics the refractive index (or index of refraction) n of a substance (optical medium) is a dimensionless number that describes how light, or any other radiation, propagates through that medium.</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/639396.rdf" xlink:title="Temperature Logger" xlink:actuate="onRequest">Pyrosciences temperature logger</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Pyrosciences temperature logger</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Pyrosciences temperature logger PI Supplied Instrument Description:Pyrosciences temperature logger that comes with the Firesting meter Instrument Name: Temperature Logger Instrument Short Name:   Instrument Description: Records temperature data over a period of time.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:operation>
          <gmi:MI_Operation>
            <gmi:description>
              <gco:CharacterString>Cruise: TI729</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/506265.rdf" xlink:title="Cruise" xlink:actuate="onRequest">TI729</gmx:Anchor>
                </gmd:code>
              </gmd:MD_Identifier>
            </gmi:identifier>
            <gmi:status>
              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://www.whoi.edu/main/ships/tioga" xlink:actuate="onRequest">R/V Tioga</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/3313" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
            <gmd:date gco:nilReason="unknown"/>
          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54181.rdf"
           xlink:title="3313" xlink:actuate="onRequest">R/V Tioga</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54181.rdf" xlink:title="R/V Tioga" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
                </gmi:status>
                <gmi:citation>
                  <gmd:CI_Citation>
                    <gmd:title>
                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/506265.rdf" xlink:title="Cruise" xlink:actuate="onRequest">TI729</gmx:Anchor>
                    </gmd:title>
                    <gmd:date gco:nilReason="unknown"/>
                    <gmd:citedResponsibleParty>
                      <gmd:CI_ResponsibleParty>
                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/51589.rdf" xlink:actuate="onRequest">Amy Elizabeth Maas</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/2.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">Woods Hole Oceanographic Institution</gmx:Anchor>
                        </gmd:organisationName>
                        <gmd:contactInfo>
                        <gmd:CI_Contact>
                          <gmd:onlineResource>
                            <gmd:CI_OnlineResource>
                              <gmd:linkage>
                                <gmd:URL>http://bcodata.whoi.edu/GoME_Pteropods/cruise_reports/Tioga729_Cruise_Report.pdf</gmd:URL>
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                              <gmd:description>
                                <gco:CharacterString>Report describing TI729</gco:CharacterString>
                              </gmd:description>
                            </gmd:CI_OnlineResource>
                          </gmd:onlineResource>
                        </gmd:CI_Contact>
                      </gmd:contactInfo>
                      <gmd:role>
                        <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="principalInvestigator"/>
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                      </gmd:CI_ResponsibleParty>
                    </gmd:citedResponsibleParty>
                   </gmd:CI_Citation>
                </gmi:citation>
              </gmi:MI_Plan>
            </gmi:plan>
            </gmi:MI_Operation>
      </gmi:operation><gmi:operation>
          <gmi:MI_Operation>
            <gmi:description>
              <gco:CharacterString>Cruise: TI746</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/517985.rdf" xlink:title="Cruise" xlink:actuate="onRequest">TI746</gmx:Anchor>
                </gmd:code>
              </gmd:MD_Identifier>
            </gmi:identifier>
            <gmi:status>
              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://www.whoi.edu/main/ships/tioga" xlink:actuate="onRequest">R/V Tioga</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/3313" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
            <gmd:date gco:nilReason="unknown"/>
          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54181.rdf"
           xlink:title="3313" xlink:actuate="onRequest">R/V Tioga</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54181.rdf" xlink:title="R/V Tioga" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
                </gmi:status>
                <gmi:citation>
                  <gmd:CI_Citation>
                    <gmd:title>
                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/517985.rdf" xlink:title="Cruise" xlink:actuate="onRequest">TI746</gmx:Anchor>
                    </gmd:title>
                    <gmd:date gco:nilReason="unknown"/>
                    <gmd:citedResponsibleParty>
                      <gmd:CI_ResponsibleParty>
                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/51589.rdf" xlink:actuate="onRequest">Amy Elizabeth Maas</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/2.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">Woods Hole Oceanographic Institution</gmx:Anchor>
                        </gmd:organisationName>
                        <gmd:contactInfo>
                        <gmd:CI_Contact>
                          <gmd:onlineResource>
                            <gmd:CI_OnlineResource>
                              <gmd:linkage>
                                <gmd:URL>http://bcodata.whoi.edu/GoME_Pteropods/cruise_reports/Tioga746_Cruise_Report_V3.pdf</gmd:URL>
                              </gmd:linkage>
                              <gmd:description>
                                <gco:CharacterString>Report describing TI746</gco:CharacterString>
                              </gmd:description>
                            </gmd:CI_OnlineResource>
                          </gmd:onlineResource>
                        </gmd:CI_Contact>
                      </gmd:contactInfo>
                      <gmd:role>
                        <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="principalInvestigator"/>
                      </gmd:role>
                      </gmd:CI_ResponsibleParty>
                    </gmd:citedResponsibleParty>
                   </gmd:CI_Citation>
                </gmi:citation>
              </gmi:MI_Plan>
            </gmi:plan>
            </gmi:MI_Operation>
      </gmi:operation><gmi:operation>
          <gmi:MI_Operation>
            <gmi:description>
              <gco:CharacterString>Cruise: TI777</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/539885.rdf" xlink:title="Cruise" xlink:actuate="onRequest">TI777</gmx:Anchor>
                </gmd:code>
              </gmd:MD_Identifier>
            </gmi:identifier>
            <gmi:status>
              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
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            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://www.whoi.edu/main/ships/tioga" xlink:actuate="onRequest">R/V Tioga</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/3313" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
            <gmd:date gco:nilReason="unknown"/>
          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54181.rdf"
           xlink:title="3313" xlink:actuate="onRequest">R/V Tioga</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54181.rdf" xlink:title="R/V Tioga" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
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            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
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