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            <gco:CharacterString>Cite this dataset as: Maas, A., Tarrant, A. M., Lawson, G. (2019) Seasonal hydrography, abundance, and distribution of pteropods from MOCNESS and CTD casts during R/V Tioga cruises in the Gulf of Maine from 2013 to 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-11-05 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/780874 [access date]</gco:CharacterString>
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        <gco:CharacterString>Dataset Description: &amp;lt;p&amp;gt;Seasonal hydrography, abundance, and distribution of pteropods from MOCNESS and CTD casts during R/V Tioga cruises in the Gulf of Maine from 2013 to 2015.&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
These data were published in Maas et al. (2018) and Maas et al. (in review).&amp;lt;br /&amp;gt;
&amp;lt;br /&amp;gt;
Related Datasets:&amp;amp;nbsp;&amp;lt;br /&amp;gt;
* Pteropod shell quality: https://www.bco-dmo.org/dataset/780791&amp;lt;br /&amp;gt;
* Pteropod respiration experiments:&amp;amp;nbsp;https://www.bco-dmo.org/dataset/780886&amp;lt;/p&amp;gt; Methods and Sampling: Methodology: 

Abundance and distributional sampling of pteropods was conducted in the western Gulf of Maine (GoM) at Murray Basin (42 21’ N and 69 47’ W) on various cruises aboard the R/V Tioga. During each cruise, vertically stratified net and hydrographic sampling was conducted using a standard 1/4-m2 Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS; Wiebe et al., 1985) with 150-m mesh nets that was towed at 8 discrete depths throughout the water column. All net tows were conducted during daylight hours. The upper nets consistently targeted 0–25, 25–50, 50–75, 75–100, and 100–150 m. The depths of the lower three nets were chosen adaptively during each cruise based on transmissometer profiles such that the deepest two nets sampled exclusively within the benthic nepheloid layer, in order to examine any associations of pteropods with the particular chemistry of this bottom resuspension zone (see Wang et al. 2017). Zooplankton samples were preserved in 70% ethanol for later enumeration and size classification. In the lab, splits of each MOCNESS net sample were examined under a lighted stereomicroscope and L. retroversa were enumerated based on size class (&amp;lt;0.5 mm, 0.5-1 mm, 1-3 mm and &amp;gt;3 mm) based on the generalized developmental stage size categories developed by Hsiao (1939).

CTD casts were also routinely conducted at the Murray Basin site using 3-L Niskin bottles and a SBE3/SBR4 sensor set, to characterizing the local hydrography (salinity, temperature, fluorescence, dissolved oxygen, and beam transmission) and carbonate chemistry. Depths for bottle sampling were chosen based on station water depth with a typical profile sampling the upper 100 m depths at 10 m intervals, the 100-200 m depths at 20 m intervals, and less frequently below. Samples for dissolved inorganic carbon (DIC) and total alkalinity (TA) were collected in 250 mL Pyrex borosilicate glass bottles following the best practice of seawater CO2 measurements (Dickson et al. 2007). Air head space of about one percent of the bottle volume was left to allow room for expansion. Each sample was then poisoned with 100 L of saturated mercuric chloride, capped with an Apiezon-L greased stopper, thoroughly mixed, and then tied with a rubber band over the glass stopper.


Sampling and analytical procedures: 

For lab measurements of carbonate chemistry, DIC was measured using an Apollo SciTech DIC auto-analyzer, while TA was measured using an Apollo SciTech alkalinity auto-titrator, a Ross combination pH electrode, and a pH meter (ORION 3 Star) based on a modified Gran titration method (detailed in Wang et al., 2017). pH and aragonite saturation state (ΩAr) were calculated from bottle sample measurements and concurrent temperature and salinity measures from the CTD cast using the CO2SYS program by Pierrot et al. (2006) with constants from Mehrbach (1973) as refit by Dickson and Millero (1987).</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/55175.rdf" xlink:title="OCE-1316040" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316040 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1316040</gmx:Anchor>
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                            <gco:CharacterString>NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels &quot;Hang On&quot; Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;This project will involve a series of five short cruises in 2013 and 2014, during which a variety of hydrographic, chemical, and biological data and samples will be collected, as well as a number of laboratory experiments examining pteropod physiology and gene expression.&lt;/p&gt;
&lt;p&gt;From NSF proposal abstract:&lt;/p&gt;
&lt;p&gt;Dissolution of excess anthropogenic CO2 into the ocean is causing the  marine environment to decrease in pH. This &quot;ocean acidification&quot; is  predicted to threaten a broad variety of marine organisms, particularly  calcifying animals such as the thecosome (i.e., shelled) pteropods.  These pelagic gastropods form an aragonite shell, are prey for a number  of commercially important fish, and are significant contributors to  carbon biogeochemistry. Their ecosystem importance, abundance, and  sensitivity to dissolution position them as an important group for  investigating the impacts of acidification. Our understanding of the  effect of high CO2 on pteropods and the pelagic ecosystem, however, is  limited primarily to short-term studies of adult calcification and  respiration response in the polar ecosystems. There have been no  seasonal studies of sensitivity and our understanding of the effect of  CO2 on pteropod early life stages is limited. Limacina retroversa is a  particularly abundant thecosome pteropod in the North Atlantic, where it  is prey for a number of fisheries species and other top predators. This  species is also the most common pteropod in the Gulf of Maine (GoM)  where it is present year round. L. retroversa thus offers the prospect  of a useful model pteropod species, given both its ecological importance  and its abundance in readily accessible waters. The investigators will  conduct a series of short cruises to sample L. retroversa on a seasonal  basis from local waters of the GoM near Cape Cod. The carbonate  chemistry of the GoM fluctuates seasonally, providing the  opportunity to assess the response of wild caught pteropods to natural  changes in CO2. By characterizing the carbonate chemistry of the water  column and measuring the metabolic rate, shell quality, and gene  expression of pteropods throughout the year, the researchers will  achieve a time series of pteropod sensitivity to CO2. Subsequently,  using experimental manipulations the investigators will explore the  effect of seasonal acclimation on pteropod response to short- and  medium-term exposure to enhanced CO2. Pteropods frequently lay eggs in  captivity, and at WHOI there is institutional expertise in maintaining  these individuals in the laboratory. Building on these strengths, the  researchers will also study the effect of CO2 on embryonic and larval  development in L. retroversa. These earliest life-stages of marine  calcifiers are thought to be especially sensitive since initial shell  precipitation and the highly energetic processes of growth and  development are impeded by CO2 exposure. They will also document  mortality, shell production, abnormality, and developmental rate of  clutches of pteropod embryos exposed to increased CO2.&lt;/p&gt;
&lt;p&gt;Intellectual  Merit: Thecosome pteropods are an abundant group of calcifying  zooplankters that have been chronically understudied, particularly in  temperate regions. Due to its accessibility and ecological importance,  L. retroversa can be developed as a valuable model, interesting both as  the dominant pteropod in the commercially-important GoM region and also  an abundant pteropod in the temperate waters of the North Atlantic. The  goal of this research is to augment our knowledge of the distribution of  L. retroversa, to attain an understanding of their seasonal sensitivity  to natural variability in CO2, and to see how this exposure impacts  responses to both short- and medium-term CO2 exposure. Using powerful  transcriptomic technologies, the research will transform our  understanding of this group by investigating the molecular mechanisms of  response in L. retroversa to both seasonality and varying durations and  intensities of acidification, contextualized by ecosystem- and  organism-level metrics. Furthermore the study will examine the  effect of CO2 on the eggs of pteropods for the first time, providing  insight into their sensitivity to an acidifying environment.&lt;/p&gt;</gco:CharacterString>
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	Name: Year
	Units: unitless
	Description: &lt;p&gt;Year for cruise of collection&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781161.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/781163.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/781164.rdf
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	Description: &lt;p&gt;Tioga cruise number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781165.rdf
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	Units: decimal degrees
	Description: &lt;p&gt;Latitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781166.rdf
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	Description: &lt;p&gt;Longitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781167.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/781168.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/781169.rdf
	Name: max
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http://lod.bco-dmo.org/id/dataset-parameter/781170.rdf
	Name: min
	Units: meters (m)
	Description: &lt;p&gt;min depth of net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781171.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/781172.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/781173.rdf
	Name: Salinity
	Units: Practical Salinity Units (PSU)
	Description: &lt;p&gt;average salinity of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781174.rdf
	Name: Pressure
	Units: decibars (db)
	Description: &lt;p&gt;average pressure of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781175.rdf
	Name: Fluorescence
	Units: milligrams per cubic meter (mg m-3)
	Description: &lt;p&gt;average fluorescence of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781176.rdf
	Name: Temperature
	Units: degrees Celsius (C)
	Description: &lt;p&gt;average temperature of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781177.rdf
	Name: Turbidity
	Units: Formazin Turbidity Units (FTU)
	Description: &lt;p&gt;average turbidity of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781178.rdf
	Name: Oxygen
	Units: micromoles per kilogram (umol kg-1)
	Description: &lt;p&gt;average oxygen of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781179.rdf
	Name: Beam_Transmission
	Units: percent (%)
	Description: &lt;p&gt;average transmissivity of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781180.rdf
	Name: DIC
	Units: micromoles per kilogram (umol kg-1)
	Description: &lt;p&gt;average DIC from CTD bottles sampled within the range of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781181.rdf
	Name: TA
	Units: micromoles per kilogram (umol kg-1)
	Description: &lt;p&gt;average total alkalinity (TA) from CTD bottles sampled within the range of the MOCNESS net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781182.rdf
	Name: pH
	Units: pH scale
	Description: &lt;p&gt;calculaed pH (from measured TA,DIC,temp,salinity)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781183.rdf
	Name: Ar
	Units: dimensionless
	Description: &lt;p&gt;calculaed aragonite saturation state (from measured TA,DIC,temp,salinity)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781184.rdf
	Name: CO2
	Units: microatmospheres (uatm)
	Description: &lt;p&gt;calculaed pCO2 (from measured TA,DIC,temp,salinity)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781185.rdf
	Name: split
	Units: dimensionless
	Description: &lt;p&gt;portion of net analyzed&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781186.rdf
	Name: all
	Units: number per cubic meter (#/m3)
	Description: &lt;p&gt;total pteropod abundance in net&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781187.rdf
	Name: lt_0pt5
	Units: number per cubic meter (#/m3)
	Description: &lt;p&gt;abundance of pteropods under 0.5 mm&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781188.rdf
	Name: from_0pt5_lt_1
	Units: number per cubic meter (#/m3)
	Description: &lt;p&gt;abundance of pteropods between 0.5-1 mm&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781189.rdf
	Name: from_1_lt_3
	Units: number per cubic meter (#/m3)
	Description: &lt;p&gt;abundance of pteropods between 1-3 mm&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/781190.rdf
	Name: gt_3
	Units: number per cubic meter (#/m3)
	Description: &lt;p&gt;abundance of pteropods over 3 mm&lt;/p&gt; 
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Abundance and distributional sampling of pteropods was conducted in the western Gulf of Maine (GoM) at Murray Basin (42 21’ N and 69 47’ W) on various cruises aboard the R/V Tioga. During each cruise, vertically stratified net and hydrographic sampling was conducted using a standard 1/4-m2 Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS; Wiebe et al., 1985) with 150-m mesh nets that was towed at 8 discrete depths throughout the water column. All net tows were conducted during daylight hours. The upper nets consistently targeted 0–25, 25–50, 50–75, 75–100, and 100–150 m. The depths of the lower three nets were chosen adaptively during each cruise based on transmissometer profiles such that the deepest two nets sampled exclusively within the benthic nepheloid layer, in order to examine any associations of pteropods with the particular chemistry of this bottom resuspension zone (see Wang et al. 2017). Zooplankton samples were preserved in 70% ethanol for later enumeration and size classification. In the lab, splits of each MOCNESS net sample were examined under a lighted stereomicroscope and L. retroversa were enumerated based on size class (&amp;lt;0.5 mm, 0.5-1 mm, 1-3 mm and &amp;gt;3 mm) based on the generalized developmental stage size categories developed by Hsiao (1939).

CTD casts were also routinely conducted at the Murray Basin site using 3-L Niskin bottles and a SBE3/SBR4 sensor set, to characterizing the local hydrography (salinity, temperature, fluorescence, dissolved oxygen, and beam transmission) and carbonate chemistry. Depths for bottle sampling were chosen based on station water depth with a typical profile sampling the upper 100 m depths at 10 m intervals, the 100-200 m depths at 20 m intervals, and less frequently below. Samples for dissolved inorganic carbon (DIC) and total alkalinity (TA) were collected in 250 mL Pyrex borosilicate glass bottles following the best practice of seawater CO2 measurements (Dickson et al. 2007). Air head space of about one percent of the bottle volume was left to allow room for expansion. Each sample was then poisoned with 100 L of saturated mercuric chloride, capped with an Apiezon-L greased stopper, thoroughly mixed, and then tied with a rubber band over the glass stopper.


Sampling and analytical procedures: 

For lab measurements of carbonate chemistry, DIC was measured using an Apollo SciTech DIC auto-analyzer, while TA was measured using an Apollo SciTech alkalinity auto-titrator, a Ross combination pH electrode, and a pH meter (ORION 3 Star) based on a modified Gran titration method (detailed in Wang et al., 2017). pH and aragonite saturation state (ΩAr) were calculated from bottle sample measurements and concurrent temperature and salinity measures from the CTD cast using the CO2SYS program by Pierrot et al. (2006) with constants from Mehrbach (1973) as refit by Dickson and Millero (1987).</gco:CharacterString>
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Problem report: There were a few cruises where instrumentation on the MOCNESS malfunctioned (the O2 sensor or transmissometer).

BCO-DMO Data Manager Processing Notes:
* Excel file exported as csv
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values in this dataset are displayed as &amp;quot;nd&amp;quot; for &amp;quot;no data.&amp;quot;  nd is the default missing data identifier in the BCO-DMO system.
* ISO_DateTime_UTC_Start added from year, month, day, time start (local) columns.
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            <gco:CharacterString>PI Supplied Instrument Name:  PI Supplied Instrument Description:CTD casts were also routinely conducted at the Murray Basin site using 3-L Niskin bottles and a SBE3/SBR4 sensor set, to characterizing the local hydrography (salinity, temperature, fluorescence, dissolved oxygen, and beam transmission) and carbonate chemistry.  Instrument Name: CTD Sea-Bird 9 Instrument Short Name:CTD SBE 9   Instrument Description: The Sea-Bird SBE 9 is a type of CTD instrument package.  The SBE 9 is the Underwater Unit and is most often combined with the SBE 11 Deck Unit (for real-time readout using conductive wire) when deployed from a research vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911.  The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorometer, altimeter, etc.). Note that in most cases, it is more accurate to specify SBE 911 than SBE 9 since it is likely a SBE 11 deck unit was used.  more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/</gco:CharacterString>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54181.rdf"
           xlink:title="3313" xlink:actuate="onRequest">R/V Tioga</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54181.rdf" xlink:title="R/V Tioga" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
          </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
