http://lod.bco-dmo.org/id/dataset/782197
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-11-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Samples collected and their associated temperatures on an expedition to the Lost City hydrothermal field on R/V Atlantis cruise AT42-01 in September 2018
2020-03-25
publication
2020-03-25
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-03-30
publication
https://doi.org/10.26008/1912/bco-dmo.782197.1
Susan Q. Lang
University of South Carolina
principalInvestigator
William Brazelton
University of Utah
principalInvestigator
Gretchen Fruh-Green
ETH-Zurich
principalInvestigator
Deborah Kelley
University of Washington
principalInvestigator
Marvin Lilley
University of Washington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lang, S., Brazelton, W., Fruh-Green, G., Kelley, D., Lilley, M. (2020) Samples collected and their associated temperatures on an expedition to the Lost City hydrothermal field on R/V Atlantis cruise AT42-01 in September 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-03-25 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.782197.1 [access date]
Samples collected and their associated temperatures on an expedition to the Lost City hydrothermal field Dataset Description: <p>Summary of samples collected by the Hydrothermal Organic Geochemistry (HOG) sampler on AT-4201 with ROV Jason, on dives J2_1107 through J2_1111, during R/V Atlantic cruise AT42-01, September 9 - October 1, 2018.</p> Methods and Sampling: <p>Fluid samples were collected into either 2 L or 11 L kynar bags that were acid washed (10% HCl soak overnight, followed by through rinsing, overnight Milli-Q soak, and further rinsing). Sample for geochemistry were collected unammended. Samples for microbiology were pre-spiked with 13C-labeled formate, bicarbonate, or methane, or with preservative. Additional samples were collected by filtering fluids in situ through sterivex filters.</p>
<p>Average T refers to the average of the fluid temperature measured while the sample was being collected.&nbsp;Temperatures were measured with NKE Instrumentation Temperature sensors (P/N S2T6000-Ti-DH; P/N 60-07-326-002).&nbsp;"N.D." is no data.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536702 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536702
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536405 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536405
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1535962 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1535962
completed
Susan Q. Lang
University of South Carolina
803-777-8832
School of the Earth Ocean and Environment 701 Sumter St. EWS 617
Columbia
SC
29208
USA
slang@geol.sc.edu
pointOfContact
William Brazelton
University of Utah
(801) 587-9455
Department of Biology
Salt Lake City
UT
84112
USA
william.brazelton@utah.edu
pointOfContact
Gretchen Fruh-Green
ETH-Zurich
41-44-632-3794
NW E 76.2 Clausiusstrasse 25
Zurich
8092
Switzerland
frueh-green@erdw.ethz.ch
pointOfContact
Deborah Kelley
University of Washington
206-685-9556
1503 NE Boat Street Box 35790
Seattle
WA
98195
United States
dskelley@u.washington.edu
pointOfContact
Marvin Lilley
University of Washington
206-543-0859
1503 NE Boat Street Box 35790
Seattle
WA
98195
USA
lilley@uw.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_Descrip
Sample_ID
Sample_Type
Site
Date_GMT
Start_Time_GMT
End_Time_GMT
Average_T
Highest_T
Comment
ISO_DateTime_Start_GMT
ISO_DateTime_End_GMT
Latitude
Longitude
theme
None, User defined
sample description
sample identification
sample type
site
date
time of day
water temperature at measurement depth
comments
ISO_DateTime_UTC
latitude
longitude
featureType
BCO-DMO Standard Parameters
ROV Jason
instrument
BCO-DMO Standard Instruments
AT42-01
service
Deployment Activity
Woods Hole, MA to San Juan, PR; Lost City hydrothermal field
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Investigating the Lost City as an ultramafic urban center of the subseafloor, fueled by energy and carbon from the mantle
https://osprey.bco-dmo.org/project/658604
Collaborative Research: Investigating the Lost City as an ultramafic urban center of the subseafloor, fueled by energy and carbon from the mantle
<p><em>NSF Award Abstract:</em><br />
The vast majority of deep seafloor sediments are inhabited by microbial communities that survive under extreme energy limitation, with apparent generation times of centuries to millennia. Hydrothermal systems are a stark contrast to these energy-starved environments and may represent important, high-activity, 'population centers' in the oceanic subsurface. When rocks from the Earth's mantle are uplifted and exposed to water, the resulting reactions lead to acidic fluids with high concentrations of hydrogen. Under certain circumstances, small organic molecules such as methane can also form in the absence of biology. These compounds can provide energy to subseafloor microbial communities and, given the ubiquity of mantle rocks, such reactions may fuel a significant proportion of the active subsurface biosphere. The current project will characterize the microbial communities inhabiting an iconic example of this type of system, the Lost City Hydrothermal Field, using a remotely operated vehicle. The ghostly spires of Lost City are highly telegenic and have been featured in professional documentaries. The high definition underwater video footage collected during the expedition will provide the raw material for an 8 week educational training program in digital media focused on kindergarten through 12th grade high school students and undergraduate students. The resulting short documentaries will be published on YouTube and the Utah Education Network.</p>
<p>Mantle rocks comprise significant portions of the seafloor, and microbial communities hosted within them may be important mediators of carbon and energy exchange between the deep Earth and the surface biosphere. Upon tectonic uplift and exposure to water, the serpentinization of these materials releases potential energy in the form of hydrogen, methane, and heat, and further reaction of these products can sustain the abiogenic synthesis of small organic molecules. Recent studies have highlighted, however, the lack of alkalithermophiles that are capable of survival at the high pH (9-11) and elevated temperatures found in these systems. The almost complete lack of carbon dioxide (CO2) represents a second, and possibly more significant, limitation to growth. To better understand the extent of the serpentinite subsurface, this project will address the question: What limits biological activity in the serpentinite subsurface? Specifically, the proposed work will test the hypotheses: (1) microbial diversity spans a wider range of temperature-pH conditions than currently recognized and (2) the scarcity of CO2 is a key biological limitation to serpentinization-driven ecosystems that can be overcome by the metabolic activity of one or a few foundation species. These hypotheses will be tested during a 20 day (10 days on site) expedition to the Lost City Hydrothermal Field, focusing on fluids as windows to the subsurface biosphere. The sampling approach will capitalize on the differences in temperature, carbon availability, and microbial activity across the field. The analytical approach will integrate multidisciplinary techniques performed on replicate subsamples and feature the application of next-generation sequencing technologies to these marine serpentinizing fluids for the first time. This study will generate extensive sequence data from environmental DNA, environmental mRNA, and single-cell genomes, allowing us to identify the in situ expression of metabolic pathways and the genomics of active single cells. These efforts will be closely linked with a thorough characterization of carbon in these fluids that will focus on identifying available substrates (e.g. methane, CO2, organic acids) and on characterizing biomarkers that reflect specific metabolic pathways (e.g. lipids, amino acids).</p>
Lost City Limits to Life
largerWorkCitation
project
eng; USA
oceans
Woods Hole, MA to San Juan, PR; Lost City hydrothermal field
-42.1222159
-42.1187256
30.1237075
30.1299858
2018-09-16
2018-09-21
Lost City Hydrothermal Field
0
BCO-DMO catalogue of parameters from Samples collected and their associated temperatures on an expedition to the Lost City hydrothermal field on R/V Atlantis cruise AT42-01 in September 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/782208.rdf
Name: Sample_Descrip
Units: unitless
Description: <p>Sample description</p>
http://lod.bco-dmo.org/id/dataset-parameter/782209.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/782210.rdf
Name: Sample_Type
Units: unitless
Description: <p>Sample type</p>
http://lod.bco-dmo.org/id/dataset-parameter/782211.rdf
Name: Site
Units: unitless
Description: <p>Site name</p>
http://lod.bco-dmo.org/id/dataset-parameter/782212.rdf
Name: Date_GMT
Units: unitless
Description: <p>Date (GMT); format: yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/782213.rdf
Name: Start_Time_GMT
Units: unitless
Description: <p>Start time (GMT); format: HH:MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/782214.rdf
Name: End_Time_GMT
Units: unitless
Description: <p>End time (GMT); format: HH:MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/782215.rdf
Name: Average_T
Units: degrees Celsius
Description: <p>Average temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/782216.rdf
Name: Highest_T
Units: degrees Celsius
Description: <p>Highest temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/782217.rdf
Name: Comment
Units: unitless
Description: <p>Comment/notes</p>
http://lod.bco-dmo.org/id/dataset-parameter/782218.rdf
Name: ISO_DateTime_Start_GMT
Units: unitless
Description: <p>Date and time at start (GMT) formatted ISO 8601 standard; format: yyyy-mm-ddTHH:MM:SSZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/782219.rdf
Name: ISO_DateTime_End_GMT
Units: unitless
Description: <p>Date and time at end (GMT) formatted ISO 8601 standard; format: yyyy-mm-ddTHH:MM:SSZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/806888.rdf
Name: Latitude
Units: decimal degrees North
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/806890.rdf
Name: Longitude
Units: decimal degrees East
Description: <p>Longitude (negative values = West)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
19346
https://darchive.mblwhoilibrary.org/bitstream/1912/25589/1/dataset-782197_lost-city-at42-01-collected-fluids__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.782197.1
download
onLine
dataset
<p>Fluid samples were collected into either 2 L or 11 L kynar bags that were acid washed (10% HCl soak overnight, followed by through rinsing, overnight Milli-Q soak, and further rinsing). Sample for geochemistry were collected unammended. Samples for microbiology were pre-spiked with 13C-labeled formate, bicarbonate, or methane, or with preservative. Additional samples were collected by filtering fluids in situ through sterivex filters.</p>
<p>Average T refers to the average of the fluid temperature measured while the sample was being collected.&nbsp;Temperatures were measured with NKE Instrumentation Temperature sensors (P/N S2T6000-Ti-DH; P/N 60-07-326-002).&nbsp;"N.D." is no data.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- created Sample_Descrip column;<br />
- re-formatted date to yyyy-mm-dd;<br />
-&nbsp;added start and end date/time columns in ISO 8601 format;<br />
- replaced commas with semi-colons in the Comment column.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:The Hydrothermal Organic Geochemistry (HOG) sampler was designed to collect hydrothermal fluids for biogeochemical and microbiological analyses. It consists of seven 2-liter and two 11-liter sample chambers for natural abundance geochemistry connected to a titanium intake nozzle with an in situ temperature probe. A second titanium intake nozzle with an in situ temperature probe is connected to seven 2-liter sample chambers devoted to incubation experiments, and five Sterivex filters for trapping microbial cell-sized particles. The HOG Sampler was deployed on all but one dive and is described in more detail in the methods section of the cruise report. Temperatures were measured with NKE Instrumentation Temperature sensors (P/N S2T6000-Ti-DH; P/N 60-07-326-002). Instrument Name: ROV Jason Instrument Short Name:ROV Jason Instrument Description: The Remotely Operated Vehicle (ROV) Jason is operated by the Deep Submergence Laboratory (DSL) at Woods Hole Oceanographic Institution (WHOI). WHOI engineers and scientists designed and built the ROV Jason to give scientists access to the seafloor that didn't require them leaving the deck of the ship. Jason is a two-body ROV system. A 10-kilometer (6-mile) fiber-optic cable delivers electrical power and commands from the ship through Medea and down to Jason, which then returns data and live video imagery. Medea serves as a shock absorber, buffering Jason from the movements of the ship, while providing lighting and a bird’s eye view of the ROV during seafloor operations. During each dive (deployment of the ROV), Jason pilots and scientists work from a control room on the ship to monitor Jason’s instruments and video while maneuvering the vehicle and optionally performing a variety of sampling activities. Jason is equipped with sonar imagers, water samplers, video and still cameras, and lighting gear. Jason’s manipulator arms collect samples of rock, sediment, or marine life and place them in the vehicle’s basket or on "elevator" platforms that float heavier loads to the surface. More information is available from the operator site at URL. https://ndsf.whoi.edu/jason/
Cruise: AT42-01
AT42-01
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT42-01
Susan Q. Lang
University of South Carolina
https://datadocs.bco-dmo.org/docs/Lost_City_Limits_to_Life/data_docs/AT42-01_Cruise%20Report_reduced.pdf
Report describing AT42-01
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel