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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/784290.rdf" xlink:actuate="onRequest">Hydrographic, nutrient and oxygen data from CTD bottles and beam transmission and fluorescence data from CTD profiles during R/V Point Sur PS1809 (HRR legs 1, 2, 3) at the Gulf Mexico, Louisiana and Texas coast, Sept-Oct 2017</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Campbell, L., Henrichs, D. (2019) Hydrographic, nutrient and oxygen data from CTD bottles and beam transmission and fluorescence data from CTD profiles during R/V Point Sur PS1809 (HRR legs 1, 2, 3) at the Gulf Mexico, Louisiana and Texas coast, Sept-Oct 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-12-12 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.784290.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>HRR bottle data Dataset Description: &amp;lt;p&amp;gt;Hydrographic, nutrient and oxygen data from CTD bottles and beam transmission and fluorescence data from CTD profiles during R/V Point Sur PS1809 (HRR legs 1, 2, 3) at the Gulf Mexico, Louisiana and Texas coast, Sept-Oct 2017.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Nutrient Analysis Equipment and Techniques:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Nutrient samples were collected,&amp;amp;nbsp;filtered (0.2 µm Acropak-200 polyethersulfone filters, Pall) and frozen on board until analysis on shore up to 3 months later. Nutrient analyses (phosphate, silicate, nitrate+nitrite, nitrite, ammonium, and urea) were performed on a 6-channel Astoria-Pacific autoanalyzer using standard methods (WHPO 1994). Ammonia analyses were based on Solorzano (1969), using phenol/hypochlorite in alkaline medium with a sodium nitroprusside catalyst. Urea analyses were based on Aminot and Kerouel (1982) using diacetyl monoxime in acid solution.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Dissolved Oxygen Analysis Equipment and Techniques:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Samples were collected for dissolved oxygen analyses soon after the rosette was brought on board. Using a Tygon or silicone drawing tube, nominal 125 ml volume-calibrated iodine flasks were rinsed 3 times with minimal agitation, then filled and allowed to overflow for at least 3 flask volumes. Reagents (MnCl2 then NaI/NaOH) were added to fix the oxygen before stoppering. The flasks were shaken twice (&amp;amp;gt; 1-minute inversions) to assure thorough dispersion of the precipitate. The lip of the flask stopper was filled with ultrapure water to prevent access to atmospheric oxygen during the up to 3 hours between sample collection and analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Oxygen flask volumes were determined gravimetrically to determine flask volumes at TAMU Geochemical and Environmental Research Group (GERG). This is done once before using flasks for the first time and periodically thereafter when a suspect volume is detected.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Dissolved oxygen analyses were performed with an automated Winkler oxygen titrator (Langdon Enterprises, Miami) using amperometric end-point detection. Thiosulfate (nominally 0.01 N) was standardized against 0.01 N potassium iodate prior to sample analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Salinity Analysis Equipment and Techniques:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Salinity samples were drawn into 200 mL Kimax high-alumina borosilicate bottles, which were rinsed three times with sample prior to filling to the shoulder. The bottles were sealed with plastic insert thimbles to reduce evaporation. PSS78 salinity (UNESCO 1981) was calculated for each sample from the measured conductivity ratios.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;A Guildline Autosal 8400B salinometer (S/N 65715) was used for salinity/conductivity measurements. The salinity analyses were performed after samples had equilibrated to laboratory temperature, usually within 6 weeks after collection. The salinometer was standardized for each group of analyses using OSIL standard seawater, with frequent use of a secondary deep water standard to check for drift during runs.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/715171.rdf" xlink:title="OCE-1760620" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1760620 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1760620</gmx:Anchor>
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Hurricane Harvey is the strongest hurricane to hit the Texas coast in decades and the resulting tidal surges, flooding and terrestrial runoff have had a severe impact on the coastal ocean. The effects on the phytoplankton, the first link in the food chain, may be unprecedented. To determine how the phytoplankton community will respond to such drastic changes in salinity, nutrient inputs, and potential toxins, immediate and continuous sampling is the only way to fully capture the effects and to identify when conditions return to &quot;normal&quot;. An automated, continuous phytoplankton imaging instrument that is deployed on the Texas coast records images of the phytoplankton and permits calculation of the abundance of different species. Together with molecular information on the genes that have been &quot;turned on&quot;, or expressed, outcomes of this project will help determine the responses of individual types of phytoplankton. Extreme storms are expected to increase in frequency with future climate change, so the responses identified now will be valuable in predicting how such events will affect these primary producers, which in turn support most of the food webs in marine ecosystems, in the future.&lt;/p&gt;
&lt;p&gt;High temporal resolution observations from the Imaging FlowCytobot (IFCB) have revealed that hurricanes in the Gulf of Mexico cause drastic changes in the phytoplankton community structure. The objectives of this RAPID project are: 1) to characterize the dynamics of the phytoplankton species in relation to the environmental variables along the Texas coast; 2) to assess the short and long-term changes in the phytoplankton community; and 3) to identify the strategies of the phytoplankton community for resource acquisition. To accomplish these objectives, this project will utilize IFCB time series to follow phytoplankton community structure during the recovery period from Hurricane Harvey. In addition, two RAPID response cruises (in late September and early October) to sample at 5 sites along a transect from Galveston to Port Aransas, TX. At each station, CTD profiles and water samples from surface and the chlorophyll maximum will be collected for nutrients, carbonate chemistry, and RNA sequencing for metatranscriptomic analysis. Metatranscriptomics can provide an indication of the metabolic strategies employed and functional relationships within the plankton community in response to changes in the environment. The advantage of a metatranscriptomic approach is that the entire molecular response to the environment is captured. So, while the response of phytoplankton to increased nutrient inputs from floodwater runoff is targeted, the responses to other environmental stresses (toxics, hypoxia, acidification) are also captured. Analyses of this time series using multivariate statistical techniques, such as principal component analysis (PCA), and network analysis, a powerful technique for identifying potential interactions among taxa, will provide insights on the environmental factors and metabolic responses structuring the community during the aftermath of the hurricane.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Related data from the The Texas Observatory for Algal Succession Time-Series (TOAST) can be found at the following: &lt;/strong&gt;&lt;a href=&quot;https://toast.tamu.edu/timeline?dataset=HRR_Cruise&quot; target=&quot;_blank&quot;&gt;https://toast.tamu.edu/timeline?dataset=HRR_Cruise&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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	Name: cruise_id
	Units: unitless
	Description: &lt;p&gt;official cruise identifier (R2R)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784551.rdf
	Name: leg_name
	Units: unitless
	Description: &lt;p&gt;cruise leg name given by participants&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784552.rdf
	Name: HRR_Leg
	Units: unitless
	Description: &lt;p&gt;Leg of cruise (HRR1; HRR2; HRR3)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784553.rdf
	Name: chf_sci
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	Description: &lt;p&gt;chief scientist&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784554.rdf
	Name: Sta_Sequence
	Units: unitless
	Description: &lt;p&gt;Order of stations&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784555.rdf
	Name: Station
	Units: unitless
	Description: &lt;p&gt;Name of sampling station&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784556.rdf
	Name: Latitude
	Units: decimal degrees
	Description: &lt;p&gt;Latitude of sampling station&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784557.rdf
	Name: Longitude
	Units: decimal degrees
	Description: &lt;p&gt;Longitude of sampling station&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784558.rdf
	Name: Water_Depth
	Units: meters
	Description: &lt;p&gt;Maximum depth of bathymetry at station&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784559.rdf
	Name: ISO_DateTime_UTC
	Units: unitless
	Description: &lt;p&gt;Date and time; ISO formatted: yyyy-mm-ddTHH:MMZ&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784560.rdf
	Name: Year
	Units: unitless
	Description: &lt;p&gt;Year water samples were taken in the format yyyy&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784561.rdf
	Name: Month
	Units: mm
	Description: &lt;p&gt;Month water samples were taken&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784562.rdf
	Name: Day
	Units: dd
	Description: &lt;p&gt;Day water samples were taken&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784563.rdf
	Name: Time
	Units: unitless
	Description: &lt;p&gt;Time water samples were taken; HH:MM UTC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784564.rdf
	Name: Niskin_Bottle_id
	Units: unitless
	Description: &lt;p&gt;Niskin bottle identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784565.rdf
	Name: Bottle_Depth
	Units: meters
	Description: &lt;p&gt;Depth at which Niskin bottle was closed&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784566.rdf
	Name: Nutrient_Bottle_id
	Units: unitless
	Description: &lt;p&gt;Sample bottle number containing nutrient water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784567.rdf
	Name: NO3_umol_L
	Units: micromol/liter
	Description: &lt;p&gt;Nutrient analysis of nitrate content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784568.rdf
	Name: NO3_mg_L_N
	Units: milligrams/liter
	Description: &lt;p&gt;Nutrient analysis of nitrate content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784569.rdf
	Name: HPO4_umol_L
	Units: micromol/liter
	Description: &lt;p&gt;Nutrient analysis of hydrogen phosphate content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784570.rdf
	Name: HPO4_mg_L_P
	Units: milligrams/liter
	Description: &lt;p&gt;Nutrient analysis of hydrogen phosphate content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784571.rdf
	Name: HSIO3_umol_L
	Units: micromol/liter
	Description: &lt;p&gt;Nutrient analysis of hydrogen silicate content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784572.rdf
	Name: HSIO3_mg_L_SiO3
	Units: milligrams/liter
	Description: &lt;p&gt;Nutrient analysis of hydrogen silicate content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784573.rdf
	Name: NH4__umol_L
	Units: micromol/liter
	Description: &lt;p&gt;Nutrient analysis of ammonium content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784574.rdf
	Name: NH4_mg_L_N
	Units: milligrams/liter
	Description: &lt;p&gt;Nutrient analysis of ammonium content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784575.rdf
	Name: NO2_umol_L
	Units: micromol/liter
	Description: &lt;p&gt;Nutrient analysis of nitrogen dioxide content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784576.rdf
	Name: NO2_mg_L_N
	Units: milligrams/liter
	Description: &lt;p&gt;Nutrient analysis of nitrogen dioxide content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784577.rdf
	Name: Urea_umol_L
	Units: micromol/liter
	Description: &lt;p&gt;Nutrient analysis of urea content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784578.rdf
	Name: Urea_mg_L_N
	Units: milligrams/liter
	Description: &lt;p&gt;Nutrient analysis of urea content&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784579.rdf
	Name: NO3_NO2_uM
	Units: microMolar
	Description: &lt;p&gt;Total nitrogen present in water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784580.rdf
	Name: Salinity_Bottle_id
	Units: unitless
	Description: &lt;p&gt;Sample bottle number containing salinity water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784581.rdf
	Name: Sample_Salinity
	Units: practical salinity units
	Description: &lt;p&gt;Salinity of collected water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784582.rdf
	Name: CTD_Salinity
	Units: practical salinity units
	Description: &lt;p&gt;Salinity recorded from CTD&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784583.rdf
	Name: Oxygen_Bottle_id
	Units: unitless
	Description: &lt;p&gt;Sample bottle number containing oxygen water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784584.rdf
	Name: Burrette_Reading
	Units: unitless
	Description: &lt;p&gt;Burrette reading of oxygen water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784585.rdf
	Name: DO_mL_L
	Units: milliliters/liter
	Description: &lt;p&gt;Calculated dissolved oxygen content in water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784586.rdf
	Name: DO_mg_L
	Units: milligrams/liter
	Description: &lt;p&gt;Calculated dissolved oxygen content in water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784587.rdf
	Name: DO_mM_L
	Units: millimol/liter
	Description: &lt;p&gt;Calculated dissolved oxygen content in water sample&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784588.rdf
	Name: Salinity_derived
	Units: practical salinity units
	Description: &lt;p&gt;Derived salinity from BTL file&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784589.rdf
	Name: Potl_Temp_derived
	Units: degrees Celsius
	Description: &lt;p&gt;Derived potential temperature from BTL file&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784590.rdf
	Name: DO_derived
	Units: milliliters/liter
	Description: &lt;p&gt;Derived dissolved oxygen content from BTL file&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784591.rdf
	Name: Density_derived
	Units: kilograms/meter^3
	Description: &lt;p&gt;Derived density from BTL file&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784592.rdf
	Name: Conductivity
	Units: Siemans/meter
	Description: &lt;p&gt;Conductivity from BTL file&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784593.rdf
	Name: Beam_Transmission
	Units: unitless
	Description: &lt;p&gt;Beam transmission from BTL file (percent)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/784594.rdf
	Name: PAR_Irradiance
	Units: micromol /meter^2/second
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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Dissolved Oxygen Analysis Equipment and Techniques:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Samples were collected for dissolved oxygen analyses soon after the rosette was brought on board. Using a Tygon or silicone drawing tube, nominal 125 ml volume-calibrated iodine flasks were rinsed 3 times with minimal agitation, then filled and allowed to overflow for at least 3 flask volumes. Reagents (MnCl2 then NaI/NaOH) were added to fix the oxygen before stoppering. The flasks were shaken twice (&amp;amp;gt; 1-minute inversions) to assure thorough dispersion of the precipitate. The lip of the flask stopper was filled with ultrapure water to prevent access to atmospheric oxygen during the up to 3 hours between sample collection and analysis.&amp;lt;/p&amp;gt;

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&amp;lt;p&amp;gt;Dissolved oxygen analyses were performed with an automated Winkler oxygen titrator (Langdon Enterprises, Miami) using amperometric end-point detection. Thiosulfate (nominally 0.01 N) was standardized against 0.01 N potassium iodate prior to sample analysis.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Salinity Analysis Equipment and Techniques:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

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&amp;lt;p&amp;gt;A Guildline Autosal 8400B salinometer (S/N 65715) was used for salinity/conductivity measurements. The salinity analyses were performed after samples had equilibrated to laboratory temperature, usually within 6 weeks after collection. The salinometer was standardized for each group of analyses using OSIL standard seawater, with frequent use of a secondary deep water standard to check for drift during runs.&amp;lt;/p&amp;gt;</gco:CharacterString>
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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BCO-DMO Processing Notes:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
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