http://lod.bco-dmo.org/id/dataset/784377
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-12-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Lab study on the effect of dissolved oxygen, salinity, and temperature on mussel adhesive plaques with mussels collected from Penn Cove Shellfish in Coupeville, Washington.
2019-12-13
publication
2019-12-13
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-12
publication
https://doi.org/10.26008/1912/bco-dmo.784377.1
Emily Carrington
University of Washington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Carrington, E. (2020) Lab study on the effect of dissolved oxygen, salinity, and temperature on mussel adhesive plaques with mussels collected from Penn Cove Shellfish in Coupeville, Washington. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-12-13 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.784377.1 [access date]
Dataset Description: <p>Data generated from laboratory experiments that investigated the influence of the dissolved oxygen concentration, salinity, and temperature of seawater on plaque attachment as the material aged. Mussels (<em>M. trossulus</em>) were collected from Penn Cove Shellfish, Quilcene Bay, Quilcene, Washington, USA [47°47’42.0” N, 122°51”10.8” W] and held experimental aquaria at the University of Washington in Seattle, Washington, USA for up to 14 days. Mussels produced threads over the course of 4 hrs that were incubated in a range of dissolved oxygen, temperature, and salinity conditions for up to 12 days. Adhesive plaques were then pulled to failure to determine adhesion strength. These data accompany the manuscript (George, M.N., Pedigo, B., and Carrington, E. 2018).</p> Methods and Sampling: <p>Adult mussels (Mytilus trossulus, Gould 1850; ~4-6 cm shell length) were collected from aquaculture lines at Penn Cove Shellfish’s mussel aquaculture operation located in Quilcene Bay, Quilcene, Washington, USA (47°47’42.0” N, 122°51”10.8” W) during the winter of 2016 (December-February). Mussel were kept in 50 L aquaria for up to two weeks, filled with 0.2 µm filtered seawater and fed Shellfish Diet 1800 (Reed Mariculture, Campbell, CA) up to 5% of wet tissue mass day-1 at an algal concentration of 2000 cells ml-1. Mussels were allowed to attach to mica sheets over the course of 4 hours. Byssal threads were cut away from the animal at the proximal region’s interface with the shell. Threads from individuals that made less than three attachments were not included in a treatment group. Mica sheets with plaque attachments were stored dry at room temperature (~21°C, ~30-40% RH) for up to two weeks and then moved into treatment conditions.</p>
<p>Mussels were secured to mica plates with rubber bands and allowed to produce byssal threads for up to four hours in typical open-ocean seawater conditions (pH ~ 8.1, T ~ 10ᵒC, Sal ~ 31 PSU, O2 ~ 8 mg L-1), after which threads were cut away from the animal in the proximal region of the thread (at the shell margin). Only mussels that produced three or more attachments were included in a treatment group. A subset of threads was tested immediately, serving as a 4 hour, ‘freshly made’ control. The remainder of mica plates with attached threads were placed in one of four treatments (‘control’, ‘oxygen’, ‘temperature’, or ‘salinity’) and allowed to mature for 12 days, removing a subset of plates at 3, 5, 8, and 12 days in some cases.</p>
<p>Seawater treatments were designed to mimic open-ocean conditions in all ways but one, pushing either temperature, dissolved oxygen, or salinity to the most extreme values seen in estuarine systems that are metabolically driven by the local biota (Lowe et al. 2019). A hypoxia treatment (‘oxygen’; O2 &lt;2 mg L-1) was achieved through the injection of N2 gas into a 3 L container, using an aerator. The dissolved oxygen concentration of seawater treatments was monitored in real-time with a DirectLine DL5000 equilibrium probe (accuracy ± 1%) attached to a UDA2182 analyzer (Honeywell, Fort Washington, PA), which controlled the injection of N2 by dynamically opening a solenoid valve in-line with a nitrogen gas cylinder. A high temperature treatment (‘temperature’; T = 30°C) was achieved using a 500-Watt titanium aquarium heater and accompanying PID controller (Aquatop Aquatic Supplies, Brea, CA). A low salinity treatment (‘salinity’; &lt;1 PSU) was achieved by placing plaques in deionized water. Seawater pH and temperature were monitored in each treatment with a Honeywell Durafet III pH electrode (Martz et al. 2010; accuracy ± 0.01), while salinity was monitored with a DL4000 conductivity cell (accuracy ± 1 PSU).</p>
<p>The adhesion strength of individual plaques was determined by gripping each byssal thread in the distal region, 1 mm away from the adhesive plaque, and pulling perpendicular to the substrate until failure using a tensometer (George and Carrington, 2018). Adhesion strength (kPa) was calculated as the maximum of the force extension curve (N), normalized by the planform area of the attachment plaque measured in mm2 (Burkett et al. 2009). The adhesion strength for 3-5 plaques were averaged and reported as a single value for each mussel. During mechanical testing, the failure mode of each plaque was also visually scored as an adhesive, peeling, or tearing failure, as outlined by Young and Crisp (1982). Adhesive failure occurred when a plaque disengaged from a surface uniformly at the adhesive-substrate interface, while a peeling failure characteristically began at a single point along the outer edge of the plaque, propagating to the rest of the structure. A tearing failure was evident when a portion of the adhesive remained attached to the surface after the test had completed.</p>
<p>The stiffness of the plaque cuticle was determined by following the protocol outlined by George and Carrington (2018). Briefly, stiffness (DMT modulus) was measured using a Dimension ICON atomic force microscope (AFM), fitted with a ScanAsyst-Air probe with a silicon-nitride tip (Bruker, Billerica, MA). Prior to testing, plaques were rinsed with DI water and allowed to dry for 5 minutes. Efforts were taken to probe smooth patches away from the thread-plaque junction, avoiding the innervating roots of the thread. DMT modulus (GPa) was calculated as the slope of the force curve during tip-sample separation. To obtain a representative stiffness of the cuticle, DMT modulus was averaged over a 10 nm2 scan area, with a sampling rate of 512 per line. DMT Modulus was calibrated against a fused silica standard (Veeco, Plainview, NY). Multiple locations (3-5) were scanned for each plaque and then averaged.</p>
<p>In preparation for amino acid (AA) analysis, adhesive plaques were collected from three different seawater treatments (4 hours and 12 days in open-ocean conditions; 12 days in nitrogen infused seawater) and stored in nitrogen flushed microfuge tubes at -80ᵒC for up to 4 weeks. Acid hydrolysis was then performed in vacuo at 110°C for 48 hours in 6M HCl, with 5% phenol added to preserve DOPA residues. The hydrolysate of each plaque was flash evaporated against DI water and methanol, dissolving the precipitate in 0.02 M HCl. 100 µl of the mixture was then analyzed using an amino acid analyzer system based on ninhydrin-based chemistry (Hitachi L-8900; Tokyo, Japan). A typical spectrum obtained from the analyzer with identified peaks is presented in Figure 6a. The integral of each amino acid peak was divided by the integral of all peaks to determine the relative molar concentration of each amino acid, normalizing against a background of 0.02 M HCl and subtracting the ammonia peak.</p>
<p>Detailed methods and results are provided in George et al., 2018.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1041213 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1041213
completed
Emily Carrington
University of Washington
206-221-4676
UW Friday Harbor Laboratories 620 University Road
Friday Harbor
WA
98250
USA
ecarring@uw.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
exp_group
mussel_ID
adhesive_age
temp
oxygen
salinity
shell_length
GI
CI
failure_mode
plaque_area
max_force
adhesion_strength
DMT_loc
DMT_modulus
Asx
Thr
Ser
Glx
Gly
Ala
Cys
Val
Met
lle
Leu
DOPA
Tyr
Phe
His
Lys
Arg
Pro
theme
None, User defined
experiment id
sample identification
age
treatment
length
No BCO-DMO term
flag
aspartic acid
threonine
serine
glutamic acid
glycine
alanine
amino acid name
valine
methionine
isoleucine
leucine
tyrosine
phenylalanine
histidine
lysine
arginine
featureType
BCO-DMO Standard Parameters
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective
http://depts.washington.edu/fhl/oael.html
Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective
<p><strong>Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective</strong><br />
This award will support researchers based at the University of Washington's Friday Harbor Laboratories. The overall focus of the project is to determine how ocean acidification affects the integrity of biomaterials and how these effects in turn alter interactions among members of marine communities. The research plan emphasizes an ecomaterial approach; a team of biomaterials and ecomechanics experts will apply their unique perspective to detail how different combinations of environmental conditions affect the structural integrity and ecological performance of organisms. The study targets a diversity of ecologically important taxa, including bivalves, snails, crustaceans, and seaweeds, thereby providing insight into the range of possible biological responses to future changes in climate conditions. The proposal will enhance our understanding of the ecological consequences of climate change, a significant societal problem.</p>
<p>Each of the study systems has broader impacts in fields beyond ecomechanics. Engineers are particularly interested in biomaterials and in each system there are materials with commercial potential. The project will integrate research and education by supporting doctoral student dissertation research, providing undergraduate research opportunities via three training programs at FHL, and summer internships for talented high school students, recruited from the FHL Science Outreach Program. The participation of underrepresented groups will be broadened by actively recruiting URM and female students. Results will be disseminated in a variety of forums, including peer-reviewed scientific publications, undergraduate and graduate course material, service learning activities in K-8 classrooms, demonstrations at FHL's annual Open House, and columns for a popular science magazine.</p>
OA - Ecomaterials Perspective
largerWorkCitation
project
eng; USA
oceans
-122.8527
-122.8527
47.795
47.795
2015-12-01
2016-02-29
Friday Harbor, WA
0
BCO-DMO catalogue of parameters from Lab study on the effect of dissolved oxygen, salinity, and temperature on mussel adhesive plaques with mussels collected from Penn Cove Shellfish in Coupeville, Washington.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/785579.rdf
Name: exp_group
Units: Unitless
Description: <p>Indication of whether plaque were part of the control, hypoxia (oxygen), salinity, or temperature experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/785580.rdf
Name: mussel_ID
Units: Unitless
Description: <p>Mussel sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/785581.rdf
Name: adhesive_age
Units: Days
Description: <p>Age of adhesive plaque (time after deposition)</p>
http://lod.bco-dmo.org/id/dataset-parameter/785582.rdf
Name: temp
Units: Unitless
Description: <p>Text identifier: high or low treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/785583.rdf
Name: oxygen
Units: Unitless
Description: <p>Text identifier: high or low treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/785584.rdf
Name: salinity
Units: Unitless
Description: <p>Text identifier: high or low treatment</p>
http://lod.bco-dmo.org/id/dataset-parameter/785585.rdf
Name: shell_length
Units: cm
Description: <p>Length of major shell axis</p>
http://lod.bco-dmo.org/id/dataset-parameter/785586.rdf
Name: GI
Units: Unitless
Description: <p>Gonad Index</p>
http://lod.bco-dmo.org/id/dataset-parameter/785587.rdf
Name: CI
Units: x10^-3 g cm^-3
Description: <p>Condition Index</p>
http://lod.bco-dmo.org/id/dataset-parameter/785588.rdf
Name: failure_mode
Units: Unitless
Description: <p>Plaque failure mode. 1 = adhesive failure, 2 = peeling failure, 3 = tearing failure</p>
http://lod.bco-dmo.org/id/dataset-parameter/785589.rdf
Name: plaque_area
Units: mm^2
Description: <p>Adhesive plaque cross-sectional area</p>
http://lod.bco-dmo.org/id/dataset-parameter/785590.rdf
Name: max_force
Units: N
Description: <p>Maximum force required to dislodge plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785591.rdf
Name: adhesion_strength
Units: kPa
Description: <p>Maximum adhesion strength required to dislodge plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785592.rdf
Name: DMT_loc
Units: Unitless
Description: <p>Location of DMT modulus measurement, if applicable.</p>
http://lod.bco-dmo.org/id/dataset-parameter/785593.rdf
Name: DMT_modulus
Units: GPa
Description: <p>Stiffness of plaque cuticle as determined by AFM</p>
http://lod.bco-dmo.org/id/dataset-parameter/785594.rdf
Name: Asx
Units: Mol%
Description: <p>Asparagine or Aspartic in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785595.rdf
Name: Thr
Units: Mol%
Description: <p>Threonine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785596.rdf
Name: Ser
Units: Mol%
Description: <p>Serine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785597.rdf
Name: Glx
Units: Mol%
Description: <p>Glutamine or Glutamic acid in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785598.rdf
Name: Gly
Units: Mol%
Description: <p>Glycine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785599.rdf
Name: Ala
Units: Mol%
Description: <p>Alanine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785600.rdf
Name: Cys
Units: Mol%
Description: <p>Cysteine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785601.rdf
Name: Val
Units: Mol%
Description: <p>Valine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785602.rdf
Name: Met
Units: Mol%
Description: <p>Methionine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785603.rdf
Name: lle
Units: Mol%
Description: <p>Isoleucine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785604.rdf
Name: Leu
Units: Mol%
Description: <p>Leucine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785605.rdf
Name: DOPA
Units: Mol%
Description: <p>Dopamine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785606.rdf
Name: Tyr
Units: Mol%
Description: <p>Tyrosine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785607.rdf
Name: Phe
Units: Mol%
Description: <p>Phenylalanine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785608.rdf
Name: His
Units: Mol%
Description: <p>Histidine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785609.rdf
Name: Lys
Units: Mol%
Description: <p>Lysine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785610.rdf
Name: Arg
Units: Mol%
Description: <p>Arginine in plaque</p>
http://lod.bco-dmo.org/id/dataset-parameter/785611.rdf
Name: Pro
Units: Mol%
Description: <p>Proline in plaque</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
29824
https://darchive.mblwhoilibrary.org/bitstream/1912/26060/1/dataset-784377_mussel-adhesive-plaques-dissolved-oxygen-salinity-and-temperature__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.784377.1
download
onLine
dataset
<p>Adult mussels (Mytilus trossulus, Gould 1850; ~4-6 cm shell length) were collected from aquaculture lines at Penn Cove Shellfish’s mussel aquaculture operation located in Quilcene Bay, Quilcene, Washington, USA (47°47’42.0” N, 122°51”10.8” W) during the winter of 2016 (December-February). Mussel were kept in 50 L aquaria for up to two weeks, filled with 0.2 µm filtered seawater and fed Shellfish Diet 1800 (Reed Mariculture, Campbell, CA) up to 5% of wet tissue mass day-1 at an algal concentration of 2000 cells ml-1. Mussels were allowed to attach to mica sheets over the course of 4 hours. Byssal threads were cut away from the animal at the proximal region’s interface with the shell. Threads from individuals that made less than three attachments were not included in a treatment group. Mica sheets with plaque attachments were stored dry at room temperature (~21°C, ~30-40% RH) for up to two weeks and then moved into treatment conditions.</p>
<p>Mussels were secured to mica plates with rubber bands and allowed to produce byssal threads for up to four hours in typical open-ocean seawater conditions (pH ~ 8.1, T ~ 10ᵒC, Sal ~ 31 PSU, O2 ~ 8 mg L-1), after which threads were cut away from the animal in the proximal region of the thread (at the shell margin). Only mussels that produced three or more attachments were included in a treatment group. A subset of threads was tested immediately, serving as a 4 hour, ‘freshly made’ control. The remainder of mica plates with attached threads were placed in one of four treatments (‘control’, ‘oxygen’, ‘temperature’, or ‘salinity’) and allowed to mature for 12 days, removing a subset of plates at 3, 5, 8, and 12 days in some cases.</p>
<p>Seawater treatments were designed to mimic open-ocean conditions in all ways but one, pushing either temperature, dissolved oxygen, or salinity to the most extreme values seen in estuarine systems that are metabolically driven by the local biota (Lowe et al. 2019). A hypoxia treatment (‘oxygen’; O2 &lt;2 mg L-1) was achieved through the injection of N2 gas into a 3 L container, using an aerator. The dissolved oxygen concentration of seawater treatments was monitored in real-time with a DirectLine DL5000 equilibrium probe (accuracy ± 1%) attached to a UDA2182 analyzer (Honeywell, Fort Washington, PA), which controlled the injection of N2 by dynamically opening a solenoid valve in-line with a nitrogen gas cylinder. A high temperature treatment (‘temperature’; T = 30°C) was achieved using a 500-Watt titanium aquarium heater and accompanying PID controller (Aquatop Aquatic Supplies, Brea, CA). A low salinity treatment (‘salinity’; &lt;1 PSU) was achieved by placing plaques in deionized water. Seawater pH and temperature were monitored in each treatment with a Honeywell Durafet III pH electrode (Martz et al. 2010; accuracy ± 0.01), while salinity was monitored with a DL4000 conductivity cell (accuracy ± 1 PSU).</p>
<p>The adhesion strength of individual plaques was determined by gripping each byssal thread in the distal region, 1 mm away from the adhesive plaque, and pulling perpendicular to the substrate until failure using a tensometer (George and Carrington, 2018). Adhesion strength (kPa) was calculated as the maximum of the force extension curve (N), normalized by the planform area of the attachment plaque measured in mm2 (Burkett et al. 2009). The adhesion strength for 3-5 plaques were averaged and reported as a single value for each mussel. During mechanical testing, the failure mode of each plaque was also visually scored as an adhesive, peeling, or tearing failure, as outlined by Young and Crisp (1982). Adhesive failure occurred when a plaque disengaged from a surface uniformly at the adhesive-substrate interface, while a peeling failure characteristically began at a single point along the outer edge of the plaque, propagating to the rest of the structure. A tearing failure was evident when a portion of the adhesive remained attached to the surface after the test had completed.</p>
<p>The stiffness of the plaque cuticle was determined by following the protocol outlined by George and Carrington (2018). Briefly, stiffness (DMT modulus) was measured using a Dimension ICON atomic force microscope (AFM), fitted with a ScanAsyst-Air probe with a silicon-nitride tip (Bruker, Billerica, MA). Prior to testing, plaques were rinsed with DI water and allowed to dry for 5 minutes. Efforts were taken to probe smooth patches away from the thread-plaque junction, avoiding the innervating roots of the thread. DMT modulus (GPa) was calculated as the slope of the force curve during tip-sample separation. To obtain a representative stiffness of the cuticle, DMT modulus was averaged over a 10 nm2 scan area, with a sampling rate of 512 per line. DMT Modulus was calibrated against a fused silica standard (Veeco, Plainview, NY). Multiple locations (3-5) were scanned for each plaque and then averaged.</p>
<p>In preparation for amino acid (AA) analysis, adhesive plaques were collected from three different seawater treatments (4 hours and 12 days in open-ocean conditions; 12 days in nitrogen infused seawater) and stored in nitrogen flushed microfuge tubes at -80ᵒC for up to 4 weeks. Acid hydrolysis was then performed in vacuo at 110°C for 48 hours in 6M HCl, with 5% phenol added to preserve DOPA residues. The hydrolysate of each plaque was flash evaporated against DI water and methanol, dissolving the precipitate in 0.02 M HCl. 100 µl of the mixture was then analyzed using an amino acid analyzer system based on ninhydrin-based chemistry (Hitachi L-8900; Tokyo, Japan). A typical spectrum obtained from the analyzer with identified peaks is presented in Figure 6a. The integral of each amino acid peak was divided by the integral of all peaks to determine the relative molar concentration of each amino acid, normalizing against a background of 0.02 M HCl and subtracting the ammonia peak.</p>
<p>Detailed methods and results are provided in George et al., 2018.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Data Manager Processing Notes:</p>
<p>-&nbsp;converted lat/lon listed in the description to decimal degrees for Osprey page.</p>
<p>- added a conventional header with dataset name, PI name, version date</p>
<p>- blank values in this dataset are displayed as "nd" for "no data." nd is the default missing data identifier in the BCO-DMO system.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact