<div><div>
<div><span style="font-size:13px"> A 200um plankton net net was deployed to ~20m depth, then recovered to just below surface, repeating five times. Trichodesmium colonies were hand picked into 0.2um filtered surface seawater, rinsed twice in 0.2um filtered surface seawater, and decanted onto a 0.2-4um Supor filter (indicated in sample provenance table, see dataset <a href="https://www.bco-dmo.org/dataset/787093">https://www.bco-dmo.org/dataset/787093</a>). </span><br />
</div>
<div><span style="font-size:13px">Proteins were extracted and trypsin digested in-gel following Saito et al., 2014 (Science). Peptides were analyzed by LC-MS/MS in data discovery mode on a Thermo Orbitrap Fusion.</span></div>
</div></div>
<div><p>FASTA file of sequences in Trichodesmium field metaproteomes analyzed by 2D LC-MS/MS mapped to a Trichodesmium metagenome (IMG ID 2821474806) plus cyanoGEBA species genomes (Shih et al, 2013). Samples were collected in North Atlantic surface waters, at station BATS (Bermuda Atlantic Time-series Study), and station ALOHA (A Long-Term Oligotrophic Habitat Assessment) between 2000 and 2018.</p>
<p>Related datasets:<br />
Trichodesmium field metaproteomes - peptide spectral counts: <a href="https://www.bco-dmo.org/dataset/787168">https://www.bco-dmo.org/dataset/787168</a><br />
Trichodesmium field metaproteomes - protein spectral counts: <a href="https://www.bco-dmo.org/dataset/787147">https://www.bco-dmo.org/dataset/787147</a><br />
Trichodesmium sample provenance: <a href="https://www.bco-dmo.org/dataset/787093">https://www.bco-dmo.org/dataset/787093</a> - Sample provenance file, which includes sample locations, filter sizes</p></div>
Trichodesmium field metaproteomes - sequence fasta
<div><p>Thermo proteome discoverer 2.2 was used to search the data with the SEQUEST algorithm. Statistical validation was performed in Scaffold (Proteome Software) at the 1% protein and peptide false discovery rate (FDR) levels.t</p>
<p>A publicly available <em>Trichodesium</em> metagenome was used in this search (IMG ID 2821474806) as well as the contents of the CyanoGEBA project (Shih et al, 2013). This fasta file contains the sequences that were identified in the metaproteome analysis.</p></div>
787181
Trichodesmium field metaproteomes - sequence fasta
2020-01-10T16:39:11-05:00
2020-01-10T16:39:11-05:00
2020-04-30T18:17:24-04:00
urn:bcodmo:dataset:787181
FASTA file of sequences in Trichodesmium field metaproteomes mapped to a Trichodesmium metagenome plus cyanoGEBA species genomes from samples collected in the Atlantic and Pacific Ocean between 2000 and 2018
FASTA file of sequences in Trichodesmium field metaproteomes analyzed by 2D LC-MS/MS mapped to a Trichodesmium metagenome (IMG ID 2821474806) plus cyanoGEBA species genomes (Shih et al, 2013). Samples were collected in North Atlantic surface waters, at station BATS (Bermuda Atlantic Time-series Study), and station ALOHA (A Long-Term Oligotrophic Habitat Assessment) between 2000 and 2018.
Related datasets:
Trichodesmium field metaproteomes - peptide spectral counts: https://www.bco-dmo.org/dataset/787168
Trichodesmium field metaproteomes - protein spectral counts: https://www.bco-dmo.org/dataset/787147
Trichodesmium sample provenance: https://www.bco-dmo.org/dataset/787093 - Sample provenance file, which includes sample locations, filter sizes
false
Saito, M. (2020) FASTA file of sequences in Trichodesmium field metaproteomes mapped to a Trichodesmium metagenome plus cyanoGEBA species genomes from samples collected in the Atlantic and Pacific Ocean between 2000 and 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-01-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.787181.1 [access date]
true
1
10.26008/1912/bco-dmo.787181.1
false
Trichodesmium
metaproteomics
2020-01-14
Datapackage.json
Frictionless Data Package
https://www.bco-dmo.org/dataset/787181/datapackage.json
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PDF
https://www.bco-dmo.org/dataset/787181/Dataset_description.pdf
application/pdf
ISO 19115-2 (NOAA Profile)
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application/xml
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application/xml
http://purl.org/dc/elements/1.1/
787181
http://lod.bco-dmo.org/id/dataset/787181
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