High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico

Website: https://www.bco-dmo.org/dataset/789061
Data Type: Cruise Results
Version: 1
Version Date: 2020-02-03

Project
» Collaborative Research: A RAPID response to Hurricane Harvey's impacts on coastal carbon cycle, metabolic balance and ocean acidification (HarveyCarbonCycle)
ContributorsAffiliationRole
Lohrenz, StevenUniversity of Massachusetts Dartmouth (UMass Dartmouth)Principal Investigator
Cai, Wei-JunUniversity of DelawareCo-Principal Investigator
Chakraborty, SumitMote Marine Laboratory (Mote)Co-Principal Investigator
Soenen, KarenWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager

Abstract
High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico.


Coverage

Spatial Extent: N:29.4883 E:-90.2333 S:28.375 W:-93.4167
Temporal Extent: 2017-09-18 - 2017-10-10

Dataset Description

High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico.

These data have been published in NASA SeaBASS Data Archive:


Acquisition Description

Seawater samples for pigment analyses were immediately filtered (2 to 5 l volume) onto Whatman 47 mm GF/F filters under low vacuum (<0.5 atm). The filters were blotted dry, and stored in 2 ml cryotubes (Fisher) in liquid nitrogen until analysis. Analyses were performed within 6 months to a year of sampling.

Extraction procedures and HPLC analytical methods follow those described in Thomas (2012) and HPLC_Method_Summary.pdf (see related files and publications). 


Processing Description

HPLC samples were analyzed during August-September 2018 at the Horn Point Analytical Laboratory by Meg Maddox (mmaddox@umces.edu). Values of -8888 indicate concentrations that were below detection levels. Values of -9999 indicate missing data. Coefficient of variation (replicate filter precision) for all NSF_Gulf_Rapid_HPLC samples was TChl a = 3.09%; Ppig = 4.28%.

The HPLC was controlled by Agilent Chemstation software.


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Related Publications

Thomas, C. S. (2012), The HPL Method. In S.B. Hooker (Ed), The Fifth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-5) (pp. 63-72). Greenbelt, MD: National Aeronautics and Space Administration. [details]

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Parameters

ParameterDescriptionUnits
Hpl_idLab Sample ID unitless
SampleCruise Sample ID unitless
StationStation unitless
VolfiltVolume Filtered liter (L)
DepthSample Depth meter (m)
DateDate (yyyymmdd) in UTC unitless
TimeTime (hh:mm:ss) unitless
LatLatitude (N) decimal degrees
LonLongitude (E) decimal degrees
Tot_Chl_aDV_Chl_a + MV_Chl_a + Chlide_a + Chl_a_allom + Chl_a_prime milligram per cubic meter (mg/m^3)
Tot_Chl_bDV_Chl_b + MV_Chl_b milligram per cubic meter (mg/m^3)
Tot_Chl_cchl_c1 + chl_c2 (i.e.: chl_c1c2) + chl_c3 milligram per cubic meter (mg/m^3)
alpha_beta_CarAlpha (Beta;epsilon) + Beta (Beta;beta) Carotenes milligram per cubic meter (mg/m^3)
But_fuco19'-Butanoyloxyfucoxanthin milligram per cubic meter (mg/m^3)
Hex_fuco19'-Hexanoyloxyfucoxanthin milligram per cubic meter (mg/m^3)
AlloAlloxanthin milligram per cubic meter (mg/m^3)
DiadinoDiadinoxanthin milligram per cubic meter (mg/m^3)
DiatoDiatoxanthin milligram per cubic meter (mg/m^3)
FucoFucoxanthin milligram per cubic meter (mg/m^3)
PeridPeridin milligram per cubic meter (mg/m^3)
ZeaZeaxanthin milligram per cubic meter (mg/m^3)
MV_Chl_aMonovinyl Chorophyll a milligram per cubic meter (mg/m^3)
DV_Chl_aDivinyl Chorophyll a milligram per cubic meter (mg/m^3)
Chlide_aChlorophyllide a milligram per cubic meter (mg/m^3)
MV_Chl_bMonovinyl Chorophyll b milligram per cubic meter (mg/m^3)
DV_Chl_bDivinyl Chorophyll b milligram per cubic meter (mg/m^3)
Chl_c1c2Chlorophyll c1 + c2 + Mg 2;4 divinyl pheoporphyrin a5 monomethyl ester milligram per cubic meter (mg/m^3)
Chl_c3Chlorophyll c3 milligram per cubic meter (mg/m^3)
LutLutein milligram per cubic meter (mg/m^3)
NeoNeoxanthin milligram per cubic meter (mg/m^3)
ViolaViolaxanthin milligram per cubic meter (mg/m^3)
Phytin_aPheophytin a milligram per cubic meter (mg/m^3)
Phide_aPheophorbide a milligram per cubic meter (mg/m^3)
PrasPrasinoxanthin milligram per cubic meter (mg/m^3)
GyroGyroxanthin milligram per cubic meter (mg/m^3)
TChlTot_Chl_a + Tot_Chl_b + Tot_Chl_c milligram per cubic meter (mg/m^3)
PPCPhotoprotective carotenoids (Allo + Diadino + Diato + Zeo + alpha-beta-Car) milligram per cubic meter (mg/m^3)
PSCPhotosynthetic carotenoids (But-fuco + Fuco + Hex-fuco + Perid) milligram per cubic meter (mg/m^3)
PSPPhotosynthetic pigments (PSC + Tchl) milligram per cubic meter (mg/m^3)
TcarTotal Carotenoids (PPC + PSC) milligram per cubic meter (mg/m^3)
TaccTotal accessory pigments (PPC + PSC + Tot_Chl_b + Tot_Chl_c) milligram per cubic meter (mg/m^3)
TpgTotal pigment concentration milligram per cubic meter (mg/m^3)
DPTotal diagnostic pigments (PSC + Allo + Zea + Tot_Chl_b) milligram per cubic meter (mg/m^3)
Tacc_TchlaRatio of Tacc to Tot_Chl_a unitless
PSC_TcarRatio of PSC to Tcar unitless
PPC_TcarRatio of PPC to Tcar unitless
TChl_TcarRatio of Tchl to Tcar unitless
PPC_TpgRatio of PPC to Tpg unitless
PSP_TpgRatio of PSC to Tpg unitless
TChla_TpgRatio of Tot_chl_a to Tpg unitless
CruiseCruise ID (AC18_12_Roberts or PE18-11) unitless
ISO_DateTime_UTCDate and time (UTC) in ISO format (yyyy-mm-ddThh:mm:ss)   unitless


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Instruments

Dataset-specific Instrument Name
Agilent RR1200 HPLC
Generic Instrument Name
High Performance Liquid Chromatograph
Dataset-specific Description
Agilent RR1200 HPLC controlled bu Agilent Chemstation software
Generic Instrument Description
A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.


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Deployments

AC18_12_Roberts

Website
Platform
R/V Acadiana
Start Date
2017-09-17
End Date
2017-09-21

PE18-11

Website
Platform
R/V Pelican
Start Date
2017-09-28
End Date
2017-10-11


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Project Information

Collaborative Research: A RAPID response to Hurricane Harvey's impacts on coastal carbon cycle, metabolic balance and ocean acidification (HarveyCarbonCycle)

Coverage: Northwestern Gulf of Mexico


NSF Award Abstract: Understanding how extreme events, like hurricanes, impact coastal ecosystems and the cycling of elements like carbon and oxygen, is important for improving our ability to predict how the global carbon cycle will respond to climate. This team of investigators, who have already been working together on understanding the carbon cycle in the Gulf of Mexico continental shelves, have important recent data against which to measure the effects of the passage of Hurricane Harvey in August, 2017. They will sample the waters and sediments of the northwestern Gulf of Mexico in September, October, and January to assess Harvey's impacts on a timescale of weeks to months. The researchers pose three specific questions: 1. Will the region become a major source of carbon dioxide to the atmosphere, releasing carbon accumulated in the bottom water and sediments, and will this potential impact be faster and greater than during normal fall and winter mixing events? Will this process acidify the surface water and for how long? 2. Will the metabolic balance be substantially pushed toward net heterotrophy as a result of the storm in comparison to other years? 3. Can the amount of material delivered or redeposited across the continental shelf by a tropical cyclone be considerably larger than that related to winter storm systems? The PIs will measure water column nutrients, oxygen, organic carbon, and inorganic carbon system parameters; determine water column and benthic metabolic and nutrient flux rates; and sediment organic matter deposition rates. They will also collect end member river samples. They will compare the immediate (mid-Sept) but limited post-hurricane data and one-month post-hurricane, more detailed data with those collected in July and April to study the impacts of the storms. they will also compare 2017-2018 seasonal data to seasonal data over the same region collected in the past (2006-2008 and 2009-2010). They will also compare the impacts of Hurricane Harvey to those of Hurricanes Katrina and Rita (2005) and Tropical Storm Cindy (June 2017). The project will involve graduate and postdoctoral research and work to communicate results to the public.


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Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)
NSF Division of Ocean Sciences (NSF OCE)

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