http://lod.bco-dmo.org/id/dataset/792478
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-02-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Grazing rates of Euphausia crystallorophias from RVIB Nathaniel B. Palmer NBP1801 in the Ross Sea, Jan.-Feb. 2018
2020-02-10
publication
2020-02-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-02-13
publication
https://doi.org/10.1575/1912/bco-dmo.792478.1
Grace Saba
Rutgers University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Saba, G. (2020) Grazing rates of Euphausia crystallorophias from RVIB Nathaniel B. Palmer NBP1801 in the Ross Sea, Jan.-Feb. 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-02-10 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.792478.1 [access date]
2018 Ross Sea E. crystallorophias grazing rates Dataset Description: <p>Four experiments using the gut fluorescence/gut evacuation technique were conducted during the R/V Nathaniel B. Palmer cruise NBP1801 in Jan-Feb 2018 to determine grazing rates of crystal krill, Euphausia crystallorophias.</p> Methods and Sampling: <p><strong>Sample collection:</strong> Mid-water trawls were conducted in the western Ross Sea, Antarctica on board the R/V Nathaniel B. Palmer in January-February 2018 (cruise number 18-01). The Issacs-Kidd Midwater Trawl (IKMT, 1.8 m frame, 500 um mesh, non-filtering cod end) was fitted with a calibrated General Oceanics flow meter. Four trawls were completed, each used to conduct a grazing experiment using the gut fluorescence/gut evacuation technique. Sampling stations for experiments 1-3 were located on the Continental Shelf Adjacent to the Ross Ice Shelf barrier near 76ºS, and experiment 4 was located on the continental shelf near 74ºS.</p>
<p>Experiment 1 date and location: 1/16/2018; -76 00.044S, 176 59.909E<br />
Experiment 2 date and location: 1/18/2018; -76 45.024S, 172 01.733E<br />
Experiment 3 date and location: 2/6/2018; -76 27.063S, 168 07.142E<br />
Experiment 4 date and location: 2/16/2018; -74 49.955S, 164 34.177E</p>
<p><strong>Experimental Procedure:</strong> Immediately after each trawl (for each experiment), a subset of crystal krill, Euphausia crystallorophias, were processed for initial gut fluorescence and the other krill were sorted into experimental buckets for a time series sample collection. The krill collected for initial gut fluorescence were immediately measured for length, wrapped in foil, flash frozen in liquid nitrogen, and placed in a -80ºC freezer for later chlorophyll gut content analysis (see below). To determine gut evacuation rates, an additional 5 krill were processed immediately after collection for the initial time point, T0. The remaining krill were quickly sorted into five buckets to achieve a total of 12 krill per bucket. Each bucket was filled with 15L of 0.2µm filtered seawater, and 4.0 mg of activated granular charcoal powder was added at a final concentration of 265 µg L-1. The filtered seawater was maintained at in situ temperature in a cold incubator room for the duration of the 24-hr experiment. One krill per bucket was removed at time points of 5, 10, 15, 20, 25, 30, 40, 50, 60, and 120 minutes, yielding a sample size of five krill per time point. Finally, 1-2 krill were removed after 24 hours to obtain background gut fluorescence which is to be subtracted from all the other krill fluorescence samples. All krill removed at each time point were measured for standard length, wrapped in foil, flash frozen in liquid nitrogen, and placed in the -80ºC freezer.</p>
<p>&nbsp;<strong>Analyses</strong>: Fluorometric analysis was then conducted on the krill samples using a Turner 10-AU Fluorometer before and after acidification. The analysis was performed in the dark to ensure that chlorophyll did not degrade during sample preparation and processing. Each krill was placed without homogenization into a 20mL glass scintillation vial where 5ml of 90% acetone was added to extract chlorophyll pigments. The samples were covered in foil and placed into the -20 ºC freezer for a 48-hr extraction. After this extraction period, the samples along with 3 prepared blanks of 5ml acetone were transferred to individual sample cuvettes. Each sample was placed in the fluorometer to obtain fluorescence values before acidification. The sample was then removed from the fluorometer, 2 drops of 1.2M hydrochloric acid was added, and the sample was placed back into the fluorometer to obtain values after acidification.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1743035 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1743035
completed
Grace Saba
Rutgers University
848-932-3466
71 Dudley Rd Room 316
New Brunswick
NJ
08901
US
saba@marine.rutgers.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Measurement
Krill_number
Time_Interval_minutes
Bucket_number
E1_Krill_length_mm
E1_Chlorophyll_a_ug_L
E1_Phaeopigment_ug_L
E2_Krill_length_mm
E2_Chlorophyll_a_ug_L
E2_Phaeopigment_ug_L
E3_Krill_length_mm
E3_Chlorophyll_a_ug_L
E3_Phaeopigment_ug_L
E4_Krill_length_mm
E4_Chlorophyll_a_ug_L
E4_Phaeopigment_ug_L
Turner 10-AU fluorometer
Isaacs-Kid Midwater Trawl
theme
None, User defined
brief description
replicate
time_elapsed
tank
length
chlorophyll a
total phaeopigment
featureType
BCO-DMO Standard Parameters
Fluorometer
Isaacs-Kidd Midwater Trawl
instrument
BCO-DMO Standard Instruments
NBP1801
service
Deployment Activity
Western Ross Sea, Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Using Bio-acoustics on an Autonomous Surveying Platform for the Examination of Phytoplankton-zooplankton and Fish Interactions in the Western Ross Sea
https://osprey.bco-dmo.org/project/788723
Using Bio-acoustics on an Autonomous Surveying Platform for the Examination of Phytoplankton-zooplankton and Fish Interactions in the Western Ross Sea
<p>NSF Award Abstract:</p>
<p>The Ross Sea is the one of the most productive regions in Antarctica and supports large populations of several key species in the Ross Sea food web, including copepods, crystal krill (Euphausia crystallorophias), and Antarctic silverfish (Pleuragramma antarcticum). Copepods and crystal krill dominate the diets of Antarctic silverfish, the dominant fish species in the high Antarctic zone, and silverfish are a major link between lower (copepods, krill) and higher (fishes, marine mammals, flighted birds, Adélie and Emperor penguins) trophic levels. Despite the significance of these key species, there is limited understanding of copepod, krill, and silverfish mesoscale distribution, spatial structure of age/maturity classes, and their interactions with physical drivers within the Ross Sea. Autonomous underwater profiling gliders are a developing technology that offers the potential for providing high spatial, temporal, and depth resolution data on regional scales. The project will test the capability of a multi-frequency echo sounder integrated into a Slocum Webb glider with the aim of providing the first glider-based acoustic assessment of simultaneous distributions of three trophic levels in the Ross Sea. Complementary glider sensors measuring physical, chemical, and biological parameters will provide mesoscale and sub-mesoscale hydrographic information from which phytoplankton-zooplankton-fish interactions and the relationships between these organisms and physics drivers (sea ice, circulation features) will be investigated. The approach proposed here, glider acoustics, is relatively new and has the potential to be transformational for investigating food webs and the Ross Sea ecosystem.</p>
<p>Researchers will modify and integrate an Acoustic Zooplankton and Fish Profiler (AZFP) multi-frequency echo sounder into a Slocum Webb G2 glider with the capability to differentiate between krill and other types of zooplankton, including copepods, and different sizes of krill and silverfish. The AZFP will be complemented with the existing glider sensors including a CTD, a WET Labs BB2FL ECO puck configured for simultaneous chlorophyll fluorescence (phytoplankton biomass) and optical backscatter measurements, and an Aanderaa Optode for measuring dissolved oxygen. The new sensor suite will be tested during a four-week glider deployment, where it will conduct acoustic surveys to map distribution and abundance of multiple zooplankton taxa and silverfish during the austral summer along the Terra Nova Bay polynya ice shelf and in adjacent continental shelf waters. The relationships between phytoplankton-zooplankton-fish distributions and the physical drivers of zooplankton and silverfish species and size distributions will be investigated. Coordinated ship-based acoustic sampling and net tows/trawls will be conducted multiple times during the glider deployment to validate glider acoustic-based species, size, and abundance measurements. Open accessible, automated data produced during this project will be made available through RUCOOL (Rutgers University Center for Ocean Observing Leadership) and THREDDS (Thematic Real-time Environmental Data Distribution System). The production of consistent, vertically-resolved, high resolution glider-based acoustic measurements will define a successful outcome of this project that should help in identifying the challenges in their use as a potentially cost-effective, automated examination of food webs in the Antarctic.</p>
bio-acoustic plankton surveys
largerWorkCitation
project
eng; USA
biota
oceans
Western Ross Sea, Southern Ocean
164.1588
172.9985
-76.7504
-74.7903
2018-01-10
2018-02-16
Terra Nova Bay, Western Ross Sea, Antarctica
0
BCO-DMO catalogue of parameters from Grazing rates of Euphausia crystallorophias from RVIB Nathaniel B. Palmer NBP1801 in the Ross Sea, Jan.-Feb. 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/792493.rdf
Name: Measurement
Units: unitless
Description: <p>The measurement as part of the gut fluorescence/gut evacuation technique that the final chlorophyll and phaeopigment data will be used for in calculations of krill grazing</p>
http://lod.bco-dmo.org/id/dataset-parameter/792494.rdf
Name: Krill_number
Units: unitless
Description: <p>Sequential number of krill used for each measurement component of the gut fluorescence/gut evacuation technique</p>
http://lod.bco-dmo.org/id/dataset-parameter/792495.rdf
Name: Time_Interval_minutes
Units: minutes
Description: <p>Time interval during each grazing experiment whereby krill were removed from replicate buckets and processed for gut fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/792496.rdf
Name: Bucket_number
Units: unitless
Description: <p>Bucket number that indicates where krill were removed at specific intervals during each grazing experiment. There were five replicate buckets; each with 12 krill during each experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/792497.rdf
Name: E1_Krill_length_mm
Units: mm = millimeters
Description: <p>Experiment 1: standard length of individual krill (anterior tip of the rostrum to posterior tip of the uropod) measured with digital calipers from the experiments that were ultimately processed for gut fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/792498.rdf
Name: E1_Chlorophyll_a_ug_L
Units: ug/L = micrograms per liter
Description: <p>Experiment 1: chlorophyll a concentration of individual krill measured with a Turner 10-AU fluorometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/792499.rdf
Name: E1_Phaeopigment_ug_L
Units: ug/L = micrograms per liter
Description: <p>Experiment 1: phaeopigment concentration of individual krill measured with a Turner 10-AU fluorometer after addition of hydrochloric acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/792500.rdf
Name: E2_Krill_length_mm
Units: mm = millimeters
Description: <p>Experiment 2: standard length of individual krill (anterior tip of the rostrum to posterior tip of the uropod) measured with digital calipers from the experiments that were ultimately processed for gut fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/792501.rdf
Name: E2_Chlorophyll_a_ug_L
Units: ug/L = micrograms per liter
Description: <p>Experiment 2: chlorophyll a concentration of individual krill measured with a Turner 10-AU fluorometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/792502.rdf
Name: E2_Phaeopigment_ug_L
Units: ug/L = micrograms per liter
Description: <p>Experiment 2: phaeopigment concentration of individual krill measured with a Turner 10-AU fluorometer after addition of hydrochloric acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/792503.rdf
Name: E3_Krill_length_mm
Units: mm = millimeters
Description: <p>Experiment 3: standard length of individual krill (anterior tip of the rostrum to posterior tip of the uropod) measured with digital calipers from the experiments that were ultimately processed for gut fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/792504.rdf
Name: E3_Chlorophyll_a_ug_L
Units: ug/L = micrograms per liter
Description: <p>Experiment 3: chlorophyll a concentration of individual krill measured with a Turner 10-AU fluorometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/792505.rdf
Name: E3_Phaeopigment_ug_L
Units: ug/L = micrograms per liter
Description: <p>Experiment 3: phaeopigment concentration of individual krill measured with a Turner 10-AU fluorometer after addition of hydrochloric acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/792506.rdf
Name: E4_Krill_length_mm
Units: mm = millimeters
Description: <p>Experiment 4: standard length of individual krill (anterior tip of the rostrum to posterior tip of the uropod) measured with digital calipers from the experiments that were ultimately processed for gut fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/792507.rdf
Name: E4_Chlorophyll_a_ug_L
Units: ug/L = micrograms per liter
Description: <p>Experiment 4: chlorophyll a concentration of individual krill measured with a Turner 10-AU fluorometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/792508.rdf
Name: E4_Phaeopigment_ug_L
Units: ug/L = micrograms per liter
Description: <p>Experiment 4: phaeopigment concentration of individual krill measured with a Turner 10-AU fluorometer after addition of hydrochloric acid</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7073
https://darchive.mblwhoilibrary.org/bitstream/1912/25373/1/dataset-792478_2018-ross-sea-crystal-krill-grazing__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.792478.1
download
onLine
dataset
<p><strong>Sample collection:</strong> Mid-water trawls were conducted in the western Ross Sea, Antarctica on board the R/V Nathaniel B. Palmer in January-February 2018 (cruise number 18-01). The Issacs-Kidd Midwater Trawl (IKMT, 1.8 m frame, 500 um mesh, non-filtering cod end) was fitted with a calibrated General Oceanics flow meter. Four trawls were completed, each used to conduct a grazing experiment using the gut fluorescence/gut evacuation technique. Sampling stations for experiments 1-3 were located on the Continental Shelf Adjacent to the Ross Ice Shelf barrier near 76ºS, and experiment 4 was located on the continental shelf near 74ºS.</p>
<p>Experiment 1 date and location: 1/16/2018; -76 00.044S, 176 59.909E<br />
Experiment 2 date and location: 1/18/2018; -76 45.024S, 172 01.733E<br />
Experiment 3 date and location: 2/6/2018; -76 27.063S, 168 07.142E<br />
Experiment 4 date and location: 2/16/2018; -74 49.955S, 164 34.177E</p>
<p><strong>Experimental Procedure:</strong> Immediately after each trawl (for each experiment), a subset of crystal krill, Euphausia crystallorophias, were processed for initial gut fluorescence and the other krill were sorted into experimental buckets for a time series sample collection. The krill collected for initial gut fluorescence were immediately measured for length, wrapped in foil, flash frozen in liquid nitrogen, and placed in a -80ºC freezer for later chlorophyll gut content analysis (see below). To determine gut evacuation rates, an additional 5 krill were processed immediately after collection for the initial time point, T0. The remaining krill were quickly sorted into five buckets to achieve a total of 12 krill per bucket. Each bucket was filled with 15L of 0.2µm filtered seawater, and 4.0 mg of activated granular charcoal powder was added at a final concentration of 265 µg L-1. The filtered seawater was maintained at in situ temperature in a cold incubator room for the duration of the 24-hr experiment. One krill per bucket was removed at time points of 5, 10, 15, 20, 25, 30, 40, 50, 60, and 120 minutes, yielding a sample size of five krill per time point. Finally, 1-2 krill were removed after 24 hours to obtain background gut fluorescence which is to be subtracted from all the other krill fluorescence samples. All krill removed at each time point were measured for standard length, wrapped in foil, flash frozen in liquid nitrogen, and placed in the -80ºC freezer.</p>
<p>&nbsp;<strong>Analyses</strong>: Fluorometric analysis was then conducted on the krill samples using a Turner 10-AU Fluorometer before and after acidification. The analysis was performed in the dark to ensure that chlorophyll did not degrade during sample preparation and processing. Each krill was placed without homogenization into a 20mL glass scintillation vial where 5ml of 90% acetone was added to extract chlorophyll pigments. The samples were covered in foil and placed into the -20 ºC freezer for a 48-hr extraction. After this extraction period, the samples along with 3 prepared blanks of 5ml acetone were transferred to individual sample cuvettes. Each sample was placed in the fluorometer to obtain fluorescence values before acidification. The sample was then removed from the fluorometer, 2 drops of 1.2M hydrochloric acid was added, and the sample was placed back into the fluorometer to obtain values after acidification.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- changed negative value for krill#49 chl-a to 0 (was -0.003, rounded to -0.0)</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner 10-AU fluorometer
Turner 10-AU fluorometer
PI Supplied Instrument Name: Turner 10-AU fluorometer PI Supplied Instrument Description:Device used specifically to detect chlorophyll fluorescence emission from each krill sample. Used to calculate krill gut fluorescence. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Isaacs-Kid Midwater Trawl
Isaacs-Kid Midwater Trawl
PI Supplied Instrument Name: Isaacs-Kid Midwater Trawl PI Supplied Instrument Description:Rectangle framed net (1.75 m x 1.44 m) with 500 um mesh and a non-filtering cod end. Used to collected zooplankton during the Ross Sea cruise. Instrument Name: Isaacs-Kidd Midwater Trawl Instrument Short Name:IKMT Instrument Description: A trawl with a pentagonal mouth opening and a dihedral depressor vane as part of the mouth opening. IKMTs come in various dimensions (refer to individual dataset documentation).
The original IKMTs were 10 foot (304 cm) and 15 foot (457 cm) at the mouth. The 10 foot IKMT net was 31 feet (9.45 m) in length (Wiebe and Benfield 2003). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/NETT0071/
Cruise: NBP1801
NBP1801
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
NBP1801
Giacomo DiTullio
College of Charleston
https://service.rvdata.us/data/cruise/NBP1801/doc/NBP1801DATA.pdf
Report describing NBP1801
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel