http://lod.bco-dmo.org/id/dataset/793501
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-02-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chlorophyll d15N in Lake Erie collected on NOAA GLERL weekly monitoring cruises from June to October 2017
2020-02-14
publication
2020-02-14
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-01-19
publication
https://doi.org/10.26008/1912/bco-dmo.793501.1
Gregory J. Dick
University of Michigan
principalInvestigator
Jenan Kharbush
Harvard University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Dick, G. J., Kharbush, J. (2021) Chlorophyll d15N in Lake Erie collected on NOAA GLERL weekly monitoring cruises from June to October 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-02-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.793501.1 [access date]
Chlorophyll d15N in Lake Erie Dataset Description: Methods and Sampling: <p><strong>Methodology:</strong><br />
Detailed sampling and analytical procedures can be found in the published paper (Kharbush et al. 2019). Briefly, nitrogen isotopes were measured in bulk organic matter and chlorophyll collected on 142mm GF/F filters (0.7 µm pore size), over the course of the summer harmful algal bloom in Lake Erie. δ<sup>15</sup>N values were corrected for a N blank originating from the HPLC solvent and from the oxidizing reagent, according to Higgins et al. (2009). Phytoplankton community composition was determined using a submersible FluoroProbe (bbe Moldaenke GmbH, Germany), which monitors in situ chlorophyll fluorescence. Isotope values were then compared with phytoplankton community composition.</p>
<p><strong>Instrumentation details:&nbsp;</strong><br />
A submersible FluoroProbe, manufactured by bbe Moldaenke GmbH, Germany, was used to monitor in situ chlorophyll fluorescence and to estimate algal community composition. Used with factory calibration settings.&nbsp;</p>
<p>Chlorophyll was purified using an Agilent 1200 series HPLC equipped with multi-wavelength UV/Vis detector and two Zorbax C18 columns (dimensions 4.6 × 250 mm, 5 μm).</p>
<p>δ15N of chlorophyll was measured using the denitrifier method (Sigman et al., 2001), on a Delta V Advantage isotope ratio mass spectrometer with a custom built purge and trap system. Isotopic measurements were standardized to the N2 reference scale using standard reference materials IAEA N3 and USGS 34.&nbsp;</p>
<p>δ15N of biomass collected on GF/Fs was analyzed on a Thermo Scientific Flash IRMS Elemental Analyzer with EA Isolink, coupled to a Delta V Advantage IRMS through a Conflo IV universal interface. Sample δ15N values were calculated using in-house laboratory standards as well as standard reference materials USGS40 and USGS41a.</p>
<p><strong>Problems reported:&nbsp;</strong>For samples 6/17/2017, 6/27/2017, and 7/6/2017 no Fluoroprobe community data are available because of an issue with calibration.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1736629 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1736629
completed
Gregory J. Dick
University of Michigan
734-763-3228
Department of Earth and Environmental Sciences 1100 N. University Ave., 2534 CC Little Building
Ann Arbor
MI
48109-1005
USA
gdick@umich.edu
pointOfContact
Jenan Kharbush
Harvard University
608-843-8517
2534 North University Building 1100 North University Avenue
Ann Arbor
MI
48109
USA
jenanjk@umich.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_date_collected
Station
Lat
Long
Bulk_biomass_d15N
Bulk_uncertainty
Corr_Chl_d15N
Corr_Chl_uncert
Epor
Epor_uncert
pcnt_Cyanos
pcnt_Greens
pcnt_Diatoms
pcnt_Crypto
FluoroProbe
Agilent 1200
Delta V Advantage
Thermo Scientific Flash IRMS Elemental Analyzer with EA Isolink
theme
None, User defined
date
station
latitude
longitude
d15N measured in biota
relative abundance
featureType
BCO-DMO Standard Parameters
Fluorometer
High-Performance Liquid Chromatograph
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
GLERL_2017
service
Deployment Activity
Western Lake Erie
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The role of heterotrophic bacteria in protecting cyanobacteria from hydrogen peroxide in coastal ecosystems
https://osprey.bco-dmo.org/project/700767
The role of heterotrophic bacteria in protecting cyanobacteria from hydrogen peroxide in coastal ecosystems
<p><em>NSF Award Abstract:</em><br />
Toxic cyanobacterial harmful algal blooms (CHABs) are now a worldwide problem that poses dangers for humans and aquatic organisms including life-threatening sickness, beach closures, health alerts, and drinking water treatment plant closures. This project focuses on the basic science needed to understand interactions between the microorganisms present in CHABs and the chemistry of the lakes they inhabit. In particular, it will study the sources, fate, and effects of hydrogen peroxide, which is a potentially important control on the toxicity and species present within these blooms. This research will be conducted in Lake Erie, a source of drinking water for 11 million people that is threatened by CHABs annually. Results will be directly integrated into two water quality models that are widely used by water managers and other stakeholders. This project will support the training of two PhD students, including a first-generation college attendee, and undergraduate students from backgrounds that are underrepresented in the earth sciences. Research will also be integrated into outreach aimed at increasing diversity in the earth sciences by involving women and underrepresented minorities in K-12 as well as college and adult educational settings.</p>
<p>The overall goal of this project is to determine the influence of hydrogen peroxide (H2O2) on cyanobacterial community composition and function in nearshore ecosystems. Preliminary results from Lake Erie show that dominant primary producers rely on heterotrophic bacteria to draw down H2O2 from transiently high environmental levels that are likely inhibitory to members of the cyanobacterial community. This suggests that H2O2 plays important and still poorly understood roles in aquatic microbial ecology. A combination of field sampling, experiments, and state-of-the art "-omics" will be used to test the overall hypothesis that H2O2 decomposition by heterotrophic "helpers" is an important determinant of microbial interactions and community structure and function. Lake Erie will be studied because (i) it is a model system for shallow coastal areas receiving high terrestrial nutrient runoff, (ii) it offers strong inshore-offshore gradients of light and nutrients for comparative studies, and (iii) existing sampling infrastructure, archived samples, and preliminary data can be leveraged. Field and laboratory experiments and measurements will be integrated to answer the following questions: Q1: What drives the temporal dynamics of H2O2 concentrations? Q2: Which enzymes and organisms are responsible for protecting the community via biological H2O2 decay? Q3: How does protection from H2O2 by helpers influence the composition and function of the community? The study will perform controlled lab experiments on cultures and on natural waters during different points of the bloom. Measures of H2O2 concentrations and rates of production and decay, along with supporting chemical and biological measurements, will be used to assess the major sources and sinks of H2O2. Molecular tools will be used to determine the pathways underpinning H2O2 decay and the effect of H2O2 on cyanobacterial community composition function. In parallel, impacts of varying H2O2 concentrations on growth rates of major cyanobacteria will be assessed experimentally. These experimental results will be placed into context through comparisons with the structure and function of microbial communities from field samples across spatial, temporal, and chemical gradients in this coastal ecosystem. The approach of integrating studies of H2O2 with "-omics" in natural systems is novel, and will advance our fundamental knowledge and understanding of the relationship between microbial community composition and function.</p>
Lake Erie H2O2
largerWorkCitation
project
eng; USA
oceans
Western Lake Erie
-83
-83
41.77
41.77
2017-06-17
2017-10-17
Western Basin of Lake Erie (41N, 83W)
0
BCO-DMO catalogue of parameters from Chlorophyll d15N in Lake Erie collected on NOAA GLERL weekly monitoring cruises from June to October 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/793649.rdf
Name: Sample_date_collected
Units: unitless
Description: <p>Date sample was collected; format: yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/793650.rdf
Name: Station
Units: unitless
Description: <p>GLERL Lake Erie Master Station name</p>
http://lod.bco-dmo.org/id/dataset-parameter/793651.rdf
Name: Lat
Units: Decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/793652.rdf
Name: Long
Units: Decimal degrees
Description: <p>Longitude; west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/793653.rdf
Name: Bulk_biomass_d15N
Units: permil (‰)
Description: <p>Average d15N value of bulk biomass collected on GF/F filters, 3 replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/793654.rdf
Name: Bulk_uncertainty
Units: 1 standard devation (‰)
Description: <p>Uncertainty in bulk d15N measurement, 3 replicates</p>
http://lod.bco-dmo.org/id/dataset-parameter/793655.rdf
Name: Corr_Chl_d15N
Units: permil (‰)
Description: <p>Chlorophyll isotope values obtained after correcting for blank and Rayleigh distillation effects</p>
http://lod.bco-dmo.org/id/dataset-parameter/793656.rdf
Name: Corr_Chl_uncert
Units: 1 standard devation (‰)
Description: <p>Uncertainty in chlorophyll values, after error propagation</p>
http://lod.bco-dmo.org/id/dataset-parameter/793657.rdf
Name: Epor
Units: permil (‰)
Description: <p>Isotopic offset between bulk and chlorophyll d15N (d15Nbulk biomass - d15Nchloropigment)</p>
http://lod.bco-dmo.org/id/dataset-parameter/793658.rdf
Name: Epor_uncert
Units: 1 standard devation (‰)
Description: <p>Uncertainty in Epor after error propagation</p>
http://lod.bco-dmo.org/id/dataset-parameter/793659.rdf
Name: pcnt_Cyanos
Units: Percent (%)
Description: <p>Depth-integrated percentage of algal community composed of cyanobacteria</p>
http://lod.bco-dmo.org/id/dataset-parameter/793660.rdf
Name: pcnt_Greens
Units: Percent (%)
Description: <p>Depth-integrated percentage of algal community composed of green algae</p>
http://lod.bco-dmo.org/id/dataset-parameter/793661.rdf
Name: pcnt_Diatoms
Units: Percent (%)
Description: <p>Depth-integrated percentage of algal community composed of diatoms</p>
http://lod.bco-dmo.org/id/dataset-parameter/793662.rdf
Name: pcnt_Crypto
Units: Percent (%)
Description: <p>Depth-integrated percentage of algal community composed of cryptophytes</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1570
https://darchive.mblwhoilibrary.org/bitstream/1912/26585/1/dataset-793501_chlorophyll-d15n-lake-erie__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.793501.1
download
onLine
dataset
<p><strong>Methodology:</strong><br />
Detailed sampling and analytical procedures can be found in the published paper (Kharbush et al. 2019). Briefly, nitrogen isotopes were measured in bulk organic matter and chlorophyll collected on 142mm GF/F filters (0.7 µm pore size), over the course of the summer harmful algal bloom in Lake Erie. δ<sup>15</sup>N values were corrected for a N blank originating from the HPLC solvent and from the oxidizing reagent, according to Higgins et al. (2009). Phytoplankton community composition was determined using a submersible FluoroProbe (bbe Moldaenke GmbH, Germany), which monitors in situ chlorophyll fluorescence. Isotope values were then compared with phytoplankton community composition.</p>
<p><strong>Instrumentation details:&nbsp;</strong><br />
A submersible FluoroProbe, manufactured by bbe Moldaenke GmbH, Germany, was used to monitor in situ chlorophyll fluorescence and to estimate algal community composition. Used with factory calibration settings.&nbsp;</p>
<p>Chlorophyll was purified using an Agilent 1200 series HPLC equipped with multi-wavelength UV/Vis detector and two Zorbax C18 columns (dimensions 4.6 × 250 mm, 5 μm).</p>
<p>δ15N of chlorophyll was measured using the denitrifier method (Sigman et al., 2001), on a Delta V Advantage isotope ratio mass spectrometer with a custom built purge and trap system. Isotopic measurements were standardized to the N2 reference scale using standard reference materials IAEA N3 and USGS 34.&nbsp;</p>
<p>δ15N of biomass collected on GF/Fs was analyzed on a Thermo Scientific Flash IRMS Elemental Analyzer with EA Isolink, coupled to a Delta V Advantage IRMS through a Conflo IV universal interface. Sample δ15N values were calculated using in-house laboratory standards as well as standard reference materials USGS40 and USGS41a.</p>
<p><strong>Problems reported:&nbsp;</strong>For samples 6/17/2017, 6/27/2017, and 7/6/2017 no Fluoroprobe community data are available because of an issue with calibration.</p>
Specified by the Principal Investigator(s)
<p>Data were&nbsp;analyzed in Microsoft Office Excel and in R Studio.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- formatted date to yyyy-mm-dd;<br />
- renamed column headers&nbsp;to conform with BCO-DMO naming conventions.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
FluoroProbe
FluoroProbe
PI Supplied Instrument Name: FluoroProbe PI Supplied Instrument Description:A submersible FluoroProbe, manufactured by bbe Moldaenke GmbH, Germany, was used to monitor in situ chlorophyll fluorescence and to estimate algal community composition. Used with factory calibration settings. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Agilent 1200
Agilent 1200
PI Supplied Instrument Name: Agilent 1200 PI Supplied Instrument Description:Chlorophyll was purified using an Agilent 1200 series HPLC equipped with multi-wavelength UV/Vis detector and two Zorbax C18 columns (dimensions 4.6 × 250 mm, 5 μm). Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Delta V Advantage
Delta V Advantage
PI Supplied Instrument Name: Delta V Advantage PI Supplied Instrument Description:δ15N of chlorophyll was measured using the denitrifier method (Sigman et al., 2001), on a Delta V Advantage isotope ratio mass spectrometer with a custom built purge and trap system. Isotopic measurements were standardized to the N2 reference scale using standard reference materials IAEA N3 and USGS 34. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Thermo Scientific Flash IRMS Elemental Analyzer with EA Isolink
Thermo Scientific Flash IRMS Elemental Analyzer with EA Isolink
PI Supplied Instrument Name: Thermo Scientific Flash IRMS Elemental Analyzer with EA Isolink PI Supplied Instrument Description:δ15N of biomass collected on GF/Fs was analyzed on a Thermo Scientific Flash IRMS Elemental Analyzer with EA Isolink, coupled to a Delta V Advantage IRMS through a Conflo IV universal interface. Sample δ15N values were calculated using in-house laboratory standards as well as standard reference materials USGS40 and USGS41a. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cruise: GLERL_2017
GLERL_2017
NOAA R4108
NOAA R4108
vessel
GLERL_2017
Gregory J. Dick
University of Michigan
NOAA R4108
NOAA R4108
vessel