19:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1a:T14c4,

Time-series bottle samples were collected from the Sage Lot Pond salt marsh tidal creek at approx. 41.5546N, 70.5071W. Samples were collected at ~0.2 m below the surface of tidal creek water every 1-2 h at the sampling site using a peristaltic or diaphragm pump for periods of a full tidal cycle (~12–14 h).Two coastal water samples were collected from the Woods Hole Oceanographic Institution Environmental Systems Laboratory intake, located about 1.6 km offshore in Vineyard Sound. Three goundwater samples were collected at the edge of Sage Lot Pond salt marsh at the elevation of 0.42 m, 0.75 m, and 1.66 m (NAVD88), corresponding to 0.56 m, 0.89 m, and 1.8 m below the land surface, respectively.

The DIC, TA, and pH collection followed standard best practice procedures outlined by Dickson et al. (2007). OrgAlk sample collection followed the TA sampling protocol. Samples were collected through purgeable capsule filters with 0.45 um pore size (Farrwest Environmental Supply, Texas, USA) into 250 mL borosilicate bottles, poisoned with 100 uL saturated mercuric chloride, sealed with a HDPE screw top or a glass stopper coated with APIEZON® – L grease, and se- cured with a rubber band. Samples for DOC analysis were filtered through 0.45 um pore size polyethersulfone cartridge filters into combusted borosilicate glass vials with Teflon-lined silicone septa caps, acidified to pH < 2 with hydrochloric acid and refrigerated until analysis.

DIC was analyzed using an Apollo SciTech DIC auto-analyzer (Model AS-C3), which uses a nondispersive infrared (NDIR) method. The sample is acidified with a 10% phosphoric acid in 10% sodium chloride solution, and CO₂ is purged with high purity nitrogen gas and measured by a LI-COR 7000 infrared analyzer (LI-COR Environmental, Nebraska, USA). Certified Reference Material (CRM) from Dr. A. Dickson at Scripps Institution of Oceanography was used to calibrate the DIC auto-analyzer at least once daily. In addition, CRM was measured as a sample every few hours to gauge and correct any potential drift. The precision and accuracy of the instrument was ~ ± 2.0 μmol kg⁻¹.

TA was measured with a Ross combination pH electrode and a pH meter (ORION 3 Star) to perform a modified Gran titration (Wang and Cai, 2004). The electrode and concentration of hydrochloric acid was calibrated every day. The CRMs were also measured as samples every few hours to correct any potential small drift. The accuracy and precision of the measurement was about 2.5±1.9 μmol kg⁻¹.

The pH samples were measured with a UV-visible spectrophotometer (Agilent 8454, Agilent Technologies, USA) at 25 ± 0.1℃ using purified meta-cresol purple (mcp) as an indicator. The pH values are reported on the total proton concentration scale and converted from 25 ± 0.1℃ to in-situ temperature using measured DIC and the CO2SYS program (van Heuven et al., 2011). The mean uncertainty of the pH measurement was ± 0.006 (range 0.0003 ‒ 0.017), calculated as the mean difference between duplicate samples.

OrgAlk concentration was determined with a digital syringe pump, a Ross combination pH electrode and a pH meter (ORION 3 Star), based on the procedure reported in Cai et al. (1998). OrgAlk sample was titrated with a calibrated HCl solution (~ 0.1 M) until the sample pH was below 3.0 (first titration). CO₂ in the sample was then removed by bubbling with high purity N₂ gas (99.999%) for ~ 10 minutes. The acidified sample was then titrated with 0.1 M NaOH solution back to its initial pH (back titration). The NaOH solution was prepared in DI water bubbled with high purity N₂ gas to prevent CO₂ dissolution into the solution. Finally, the sample was titrated with HCl again until its pH was below 3.0 (second titration). OrgAlk was calculated as the TA from the second titration minus the borate alkalinity. The mean difference of OrgAlk concentrations between duplicate samples was 2.8 ± 2.1 µmol kg⁻¹. Due to the existence of a small amount of CO₂ in the NaOH solution, the OrgAlk results were corrected by subtracting introduced carbonate alkalinity based on the volume of NaOH solution added during the back titration.

DOC samples were analyzed on an O. I. Analytical Aurora 1030C Autoanalyzer by high-temperature catalytic oxidation followed by nondispersive infrared detection (HTCO-NDIR). Concentrations are reported relative to a potassium hydrogen phthalate (KHP) standard. Hansell deep seawater (University of Miami Hansell Laboratory, Lot# 01-14), and Suwannee River NOM (IHSS, Lot# 2R101N) reference materials were analyzed daily as additional checks on precision and accuracy of the analyses. Standards and reference materials typically vary by < 5%.

Tidal water samples for practical salinity were analyzed with a Guideline AutoSal instrument at Woods Hole Oceanographic Institution. A YSI EXO2 Sonde (YSI Inc., Ohio, USA) was submerged in the tidal creek to measure temperature and water depth. Groundwater salinity and temperature were measured with a YSI Pro30 (YSI Inc., Ohio, USA) during collection.The YSI EXO2 recorded at intervals ranging from 2 min to 8 min. Reported YSI EXO2 sensor accuracy specifications are: 1% of the reading for salinity and 0.05 °C for temperature.

1b:T424,Comprehensive multi-parameter, water-quality monitoring sondes designed for long-term monitoring, profiling and spot sampling. The EXO sondes are split into several categories: EXO1 Sonde, EXO2 Sonde, EXO3 Sonde. Each category has a slightly different design purpose with the EXO2 and EXO3 containing more sensor ports than the EXO1. Data are collected using up to four user-replaceable sensors and an integral pressure transducer. Users communicate with the sonde via a field cable to an EXO Handheld, via Bluetooth wireless connection to a PC, or a USB connection to a PC. Typical parameter specifications for relevant sensors include dissolved oxygen with ranges of 0-50 mg/l, with a resolution of +/- 0.1 mg/l, an accuracy of 1 percent of reading for values between 0-20 mg/l and an accuracy of +/- 5 percent of reading for values 20-50 mg/l. Temp ranges are from-5 to +50 degC, with an accuracy of +/- 0.001 degC. Conductivity has a range of 0-200 mS/cm, with an accuracy of +/-0.5 percent of reading + 0.001 mS/cm and a resolution of 0.0001 - 0.01 mS/cm.1c:Ta73,NSF Award Abstract: Carbon production in vegetated coastal systems such as marshes is among the highest in the biosphere. Resolving carbon production from marshes and assessing their impacts on coastal carbon cycling are critical to determining the long-term impacts of global change such as ocean acidification and eutrophication. In this project, researchers will use new methods to improve the assessment of carbon production from salt marshes. The overarching goals are to understand the role of coastal wetlands in altering carbonate chemistry, alkalinity, and carbon budgets of the coastal ocean, as well as their capacity to buffer against anthropogenically driven chemical changes, such as ocean acidification. This project will involve training for undergraduate, graduate, and postdoctoral researchers, and will provide educational opportunities for students from a local Native American tribe. Tidal water, after exchange with intertidal salt marshes, contains higher total alkalinity (TA), higher carbon dioxide, but lower pH. These highly productive, vegetated wetlands are deemed to export both alkalinity and dissolved inorganic carbon (DIC) to the ocean. This creates an apppartent paradox in that salt marshes are both an acidifying and alkalizing source to the ocean. Limited studies suggest that marsh DIC and alkalinity export fluxes may be a significant player in regional and global carbon budgets, but the current estimates are still far too uncertain to be conclusive. Unfortunately, tidal marsh ecosystems have dramatically diminished in the recent past, and are likely to diminish further due to sea level rise, land development, eutrophication, and other anthropogenic pressures. To assess the potential impacts of this future change, it is imperative to understand its current status and accurately evaluate its significatce to other parts of the carbon cycle. Similarly, little is know about the distinct sources of DIC and alkalinity being exported from marshes via tidal exchange, although aerobic and various anaerobic respiration processes have been indicated. In this study, researchers will undertake an in-depth study using new methods to vastly improve export fluxes from intertidal salt marshes through tidal exchange over minutes to annual scales, characterize and evaluate the compostiion (carbonate versus non-carbonate alkalinity) of marsh exported TA, the role and significance of the DOC pool in altering carbonate chemistry and export fluxes, identify sources of DIC being exported in tidal water, and investigate how marsh export of TA and DIC impacts carbonate chemistry and the carbon and alkalinity budgets in coastal waters.6:["$","$L19",null,{"dataset":{"_id":"794163","description":"","problem":"","acquisition":"$1a","processing":"

All EXO2 sensors were cleaned and calibrated regularly according to manufacturer recommended methods to maintain performance, and antifouling measures were deployed including copper and automated wiping. After a deployment period of 2–4 weeks, YSI EXO2 data were evaluated for fouling and calibration drift. The YSI EXO2 was recalibrated and a correction factor based on calibration standards was applied linearly across the deployment as needed. A maximum correction up to ± 30% of the calibration value was allowed or otherwise discarded (Wagner et al., 2006). Salinity and temperature values were interpolated to match the same time as the bottle sample collection.

BCO-DMO Processing:
\n- added columns for sample_descrip, lat, and lon from header rows in original Excel file;
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Latitude of sample collection

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latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

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Longitude of sample collection

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Total alkalinity

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Organic alkalinity

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Dissolved organic carbon

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dissolved organic Carbon

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water temperature at measurement depth

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Water salinity

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Salinity: The amount (mass or quantity of matter) of salt per amount (volume or mass) of a body of water.

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Water elevation (North American Vertical Datum of 1988 or NAVD88)

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Sea surface vertical distance above a named surface.

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\n2009-08-30T14:05:00[.xx]Z (UTC time)
\n2009-08-30T14:05:00[.xx]-05:00
\nThe dashes and the colons can be dropped.
\nThe T can also be dropped \"by mutual agreement\", but one needs the trailing Z if the time is UTC.

Sample ISO_datetime_utc:
\n2009-08-30T14:05:00[.xx]Z (UTC time)

Format: YYYY-mm-ddTHH:MM:SS[.xx]Z (UTC time)

"}}],"related_publications":[{"id":"794173","citation":"Cai, W.-J., Wang, Y., & Hodson, R. E. (1998). Acid-Base Properties of Dissolved Organic Matter in the Estuarine Waters of Georgia, USA. Geochimica et Cosmochimica Acta, 62(3), 473–483. doi:10.1016/s0016-7037(97)00363-3\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"737040","citation":"Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. ISBN: 1-897176-07-4. URL: https://www.nodc.noaa.gov/ocads/oceans/Handbook_2007.html","relation_type":["Methods"],"relation_desc":""},{"id":"794171","citation":"Song, S., Wang, Z. A., Gonneea, M. E., Kroeger, K. D., Chu, S. N., Li, D., & Liang, H. (2020). An Important Biogeochemical Link between Organic and Inorganic Carbon Cycling: Effects of Organic Alkalinity on Carbonate Chemistry in Coastal Waters Influenced by Intertidal Salt Marshes. Geochimica et Cosmochimica Acta. doi:10.1016/j.gca.2020.02.013","relation_type":["Results"],"relation_desc":""},{"id":"794172","citation":"Van Heuven, S., Pierrot, D., Rae, J. W. B., Lewis, E., & Wallace, D. W. R. (2011). MATLAB Program Developed for CO2 System Calculations. ORNL/CDIAC-105b. Carbon Dioxide Information Analysis Center (CDIAC). https://doi.org/10.3334/CDIAC/OTG.CO2SYS_MATLAB_V1.1","relation_type":["Software"],"relation_desc":""},{"id":"768585","citation":"Wagner, R. J., Boulger, R. W., Oblinger, C. J., & Smith, B. A. (2006). Guidelines and standard procedures for continuous water-quality monitors: Station operation, record computation, and data reporting. Techniques and Methods. doi:10.3133/tm1d3\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"768626","citation":"Wang, Z. A., & Cai, W.-J. (2004). Carbon dioxide degassing and inorganic carbon export from a marsh-dominated estuary (the Duplin River): A marsh CO2pump. 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