http://lod.bco-dmo.org/id/dataset/794698 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2020-02-28 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Solution 31P NMR spectra files from sediment samples collected during cruises in the Arctic Ocean, California Margin, and Equatorial Pacific from 1992-1998 2020-02-28 publication 2020-02-28 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2020-07-02 publication https://doi.org/10.26008/1912/bco-dmo.794698.1 Adina Paytan University of California-Santa Cruz principalInvestigator Dr Delphine Defforey University of California-Santa Cruz principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Paytan, A., Defforey, D. (2020) Solution 31P NMR spectra files from sediment samples collected during cruises in the Arctic Ocean, California Margin, and Equatorial Pacific from 1992-1998. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-02-28 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.794698.1 [access date] Dataset Description: Solution 31P nuclear magnetic resonance (NMR) spectra files from sediment samples collected during cruises in the Arctic Ocean, California Margin, and Equatorial Pacific from 1992-1998. The spectra are contained in free induction decay (FID) files. These data were published in Defforey et al. (2017). See the related-resource page https://www.bco-dmo.org/related-resource/794727 for other datasets related to this publication. Sediment sample information for this dataset is available as a supplemental document (Sediment_Sample_Info.csv) which contains collection date, water depth, sediment depth, latitude, and longitude. Additional award information: * NSF C-DEBI subaward # 156246 to Adina Paytan * NSF C-DEBI subaward # 157598 to Delphine Defforey Methods and Sampling: Locations: Arctic Ocean: P-1-94-AR P21, 84°5' N, 174°58' W California margin: W-2-98-NC TF1, 41°5' N, 125°1' W Equatorial Pacific: TT013-06MC, 12°00' S, 134°56' W These procedures apply to all NMR spectra files. Prior to the extraction, we freeze-dried, ground and sieved sediment samples to less than 125 μm (Ruttenberg 1992). For a given sample, we weighed four sample replicates (2 g) and placed each in 250 mL HDPE bottles. Sodium dithionite (F.W. 147.12 g/mol; 7.4 g) was added to each sample split, followed by 200 mL of citrate-bicarbonate solution (pH 7.6). This step produces effervescence, so the solution should be added slowly to the sample. We shook samples for 8 h and then centrifuged them at 3,700 rpm for 15 min. We filtered the supernatants with a 0.4 μm polycarbonate filter. We took 20 mL aliquots from the filtrate for each sample split for MRP and total P analyses, and kept them refrigerated until analysis within 24 h. We added 200 mL of ultrapure water to the solid residue for each sample split as a wash step after the above reductive step, shook samples for 2 h, and then centrifuged them at 3,700 rpm for 15 min. We filtered the supernatants with 0.4 μm polycarbonate filters and set aside 20 mL of filtrate from each sample split for MRP and total P analyses. We then extracted the solid sample residues in 200 mL of sodium acetate buffer (pH 4.0) for 6 h. At the end of this extraction step, we centrifuged the bottles at 3,700 rpm for 15 min, filtered the supernatants with 0.4 μm polycarbonate filters and took a 20 mL aliquot of filtrate from each sample split for MRP and total P analyses. We added 200 mL of ultrapure water to the solid residue for each sample split as a wash step, shook samples for 2 h, and then centrifuged them at 3,700 rpm for 15 min. We filtered the supernatants with 0.4 μm polycarbonate filters and set aside 20 mL of filtrate from each sample split for MRP and total P analyses. We repeated the water rinse step, and collected aliquots for MRP and total P analyses as in the previous steps. The concentrations of TP were determined as described below. Solid sediment sample residues following the pretreatment described above were transferred to two 50 mL centrifuge tubes (2 sample replicates combined per tube). We added 20 mL of 0.25 M NaOH + 0.05 M Na2EDTA solution to each tube, vortexed until all sediment was resuspended and then shook samples for 6 h at room temperature (Cade-Menun et al. 2005). We used a solid to solution ratio of 1:5 for this step to minimize the amount of freeze-dried material that will need to be dissolved for the 31P NMR experiments. Large amounts of salts from the NaOH-EDTA concentrated in NMR samples lead to higher viscosity and increase line broadening on NMR spectra (Cade-Menun and Liu 2014). We chose an extraction time of 6 h to improve total P recovery while limiting the degradation of natural P compounds in the sample. At the end of the extraction, samples were centrifuged at 3,700 rpm for 15 min and supernatants decanted into 50 mL centrifuge tubes. We collected a 500 μL aliquot from each sample, which we diluted with 4.5 mL of ultrapure water. These were refrigerated until analysis for total P content on the ICP-OES. The sample residues and supernatants were frozen on a slant to maximize the exposed surface area during the lyophilization step; this was done immediately after the removal of the 500 μL aliquot. Once completely frozen, the uncapped tubes containing supernatants and residues were freeze-dried over the course of 48 h. Each tube was covered with parafilm with small holes from a tack to minimize contamination. Freeze-dried supernatants from identical sample splits were combined and dissolved in 500 μL each of ultrapure water, D2O, NaOH-EDTA and 10 M NaOH prior to 31P NMR analysis. The D2O is required as signal lock in the spectrometer (Cade-Menun and Liu 2014). Sample pH was maintained at a pH &gt; 12 to optimize peak separation (Cade-Menun 2005; Cade-Menun and Liu 2014). Sample pH was assessed with a glass electrode, and verified with pH paper to account for the alkaline error caused by the high salt content of our samples (Covington 1985). Spectra were acquired immediately following sample preparation on a Varian Unity INOVA 600 MHz spectrometer equipped with a 10-mm broadband probe [operated by the Stanford Magnetic Resonance Laboratory at Stanford University]. We used a 10-mm rather than a 5-mm probe because larger tubes contain a greater concentration of P and thus require fewer scans to achieve similar signal to noise ratios (Cade-Menun and Liu 2014). The analytical parameters used were: 20oC, 90o pulse, 0.48 s acquisition time, 4.52 s delay time, 5600 scans (8 h experiments), no spin and an external H3PO4 standard. We maintained samples at a temperature of 20oC during experiments to achieve optimal spectral resolution (Crouse et al. 2000) and to minimize sample degradation. No proton decoupling was used out of concern for sample degradation (Cade-Menun and Liu 2014). The ratio of P to Fe and manganese (Mn) was used as a proxy for spin-lattice relaxation times (T1) to ensure adequate delays between pulses and thus quantitative spectra (McDowell et al. 2006). We used 5 s recycle delays, which correspond to three to five times the calculated T1 values, as recommended by McDowell et al. (2006). Peak identification was based on literature (Turner et al. 2003; Cade-Menun 2015). 31P NMR data were processed using the NMR Utility Transform software (NUTS, Acorn NMR). Peak areas were calculated by integration of spectra processed with a 7 Hz line broadening following baseline correction, peak picking and phasing. We accepted peaks that (1) represented at least 1% of the tallest peak in the total integrated area, (2) were identified by the NUTS software and (3) were confirmed as signal by visual inspection. Freeze-dried sample residues were ashed in crucibles at 550°C for 2 h and then extracted in 25 mL of 0.5 M sulfuric acid for 16 h (Olsen and Sommers 1982; Cade-Menun and Lavkulich 1997). We centrifuged samples at 3,700 rpm for 15 min, filtered supernatants with 0.4 μm polycarbonate filters, and measured P content on an ICP-OES. Total P concentrations in sediment extracts were measured using inductively coupled plasma optical emission spectroscopy (ICP-OES). Standards were prepared with the same solutions as those used for the extraction procedure in order to minimize matrix effects on P measurements. Sediment extracts and standards (0 μM, 3.2 μM, 32 μM and 320 μM) were diluted to lower salt content to prevent salt buildup on the nebulizer (1:20 dilution for step 1, 1:10 for steps 2 4). Concentration data from both wavelengths (213 nm and 214 nm) were averaged to obtain extract concentrations for each sample. The detection limit for P on this instrument for both wavelengths is 0.4 μM. The MRP concentrations were measured on a QuikChem 8000 automated ion analyzer. Standards were prepared with the same solutions used for the extraction step to minimize matrix effects on P measurements. Sediment extracts and standards (0 30 μM PO4) were diluted ten-fold to prevent matrix interference with color development. The detection limit for P on this instrument is 0.2 μM. We derived MUP concentrations by subtracting MRP from total P concentrations. Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0939564 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0939564 completed Adina Paytan University of California-Santa Cruz 831-459-1437 Earth & Marine Sciences C308 1156 High St Santa Cruz CA 95064 USA apaytan@ucsc.edu pointOfContact Dr Delphine Defforey University of California-Santa Cruz Department of Earth and Planetary Sciences 1156 High Street Santa Cruz CA 95060 USA ddeffore@ucsc.edu pointOfContact asNeeded Dataset Version: 1 Unknown QuikChem 8000 automated ion analyzer theme None, User defined Flow Injection Analyzer instrument BCO-DMO Standard Instruments P194AR W9807A TT013 service Deployment Activity Arctic Ocean Eureka, California, Northern California U.S. JGOFS Equatorial Pacific place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Center for Dark Energy Biosphere Investigations http://www.darkenergybiosphere.org Center for Dark Energy Biosphere Investigations The mission of the Center for Dark Energy Biosphere Investigations (C-DEBI) is to explore life beneath the seafloor and make transformative discoveries that advance science, benefit society, and inspire people of all ages and origins. C-DEBI provides a framework for a large, multi-disciplinary group of scientists to pursue fundamental questions about life deep in the sub-surface environment of Earth. The fundamental science questions of C-DEBI involve exploration and discovery, uncovering the processes that constrain the sub-surface biosphere below the oceans, and implications to the Earth system. What type of life exists in this deep biosphere, how much, and how is it distributed and dispersed? What are the physical-chemical conditions that promote or limit life? What are the important oxidation-reduction processes and are they unique or important to humankind? How does this biosphere influence global energy and material cycles, particularly the carbon cycle? Finally, can we discern how such life evolved in geological settings beneath the ocean floor, and how this might relate to ideas about the origin of life on our planet? C-DEBI's scientific goals are pursued with a combination of approaches: (1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites; (2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems; (3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and (4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists. Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI. Data Management: C-DEBI is committed to ensuring all the data generated are publically available and deposited in a data repository for long-term storage as stated in their Data Management Plan (PDF) and in compliance with the NSF Ocean Sciences Sample and Data Policy. The data types and products resulting from C-DEBI-supported research include a wide variety of geophysical, geological, geochemical, and biological information, in addition to education and outreach materials, technical documents, and samples. All data and information generated by C-DEBI-supported research projects are required to be made publically available either following publication of research results or within two (2) years of data generation. To ensure preservation and dissemination of the diverse data-types generated, C-DEBI researchers are working with BCO-DMO Data Managers make data publicly available online. The partnership with BCO-DMO helps ensure that the C-DEBI data are discoverable and available for reuse. Some C-DEBI data is better served by specialized repositories (NCBI's GenBank for sequence data, for example) and, in those cases, BCO-DMO provides dataset documentation (metadata) that includes links to those external repositories. C-DEBI largerWorkCitation program A new marine sediment sample preparation scheme for solution 31P NMR analysis https://osprey.bco-dmo.org/project/664054 A new marine sediment sample preparation scheme for solution 31P NMR analysis <p>We developed and tested a new approach to prepare marine sediment samples for solution 31P nuclear magnetic resonance spectroscopy (31P NMR). This approach addresses the effects of sample pretreatment on sedimentary P composition and increases the signal of low abundance P species in 31P NMR spectra by removing up the majority inorganic P  from sediment samples while causing minimal alteration of the chemical structure of organic P compounds. The method was tested on natural marine sediment samples from different localities (Equatorial Pacific, California Margin and Arctic Ocean) with high inorganic P content, and allowed for the detection of low abundance P forms in samples for which only an orthophosphate signal could be resolved with an NaOH-EDTA extraction alone. This new approach will allow the use of 31P NMR on samples for which low organic P concentrations previously hindered the use of this tool, and will help answer longstanding question regarding the fate of organic P in marine sediments. We developed and tested a new approach to prepare marine sediment samples for solution 31P nuclear magnetic resonance spectroscopy (31P NMR). This approach addresses the effects of sample pretreatment on sedimentary P composition and increases the signal of low abundance P species in 31P NMR spectra by removing up the majority inorganic P  from sediment samples while causing minimal alteration of the chemical structure of organic P compounds. The method was tested on natural marine sediment samples from different localities (Equatorial Pacific, California Margin and Arctic Ocean) with high inorganic P content, and allowed for the detection of low abundance P forms in samples for which only an orthophosphate signal could be resolved with an NaOH-EDTA extraction alone. This new approach will allow the use of 31P NMR on samples for which low organic P concentrations previously hindered the use of this tool, and will help answer longstanding question regarding the fate of organic P in marine sediments. </p> <p>NSF C-DEBI Award #156246 to Dr. Adina Paytan</p> <p>NSF C-DEBI Award #157598 to Dr. Delphine Defforey </p> Marine Sediment Analysis 31P NMR largerWorkCitation project eng; USA oceans Arctic Ocean; Eureka, California, Northern California; U.S. JGOFS Equatorial Pacific -174.967 -125.017 -12 84.083 1992-10-30 1998-07-24 Equatorial Pacific, California Margin, Arctic Ocean 0 BCO-DMO catalogue of parameters from Solution 31P NMR spectra files from sediment samples collected during cruises in the Arctic Ocean, California Margin, and Equatorial Pacific from 1992-1998 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2331903 https://darchive.mblwhoilibrary.org/bitstream/1912/25934/1/NMR_FID_files.zip download https://doi.org/10.26008/1912/bco-dmo.794698.1 download onLine dataset Locations: Arctic Ocean: P-1-94-AR P21, 84°5' N, 174°58' W California margin: W-2-98-NC TF1, 41°5' N, 125°1' W Equatorial Pacific: TT013-06MC, 12°00' S, 134°56' W These procedures apply to all NMR spectra files. Prior to the extraction, we freeze-dried, ground and sieved sediment samples to less than 125 μm (Ruttenberg 1992). For a given sample, we weighed four sample replicates (2 g) and placed each in 250 mL HDPE bottles. Sodium dithionite (F.W. 147.12 g/mol; 7.4 g) was added to each sample split, followed by 200 mL of citrate-bicarbonate solution (pH 7.6). This step produces effervescence, so the solution should be added slowly to the sample. We shook samples for 8 h and then centrifuged them at 3,700 rpm for 15 min. We filtered the supernatants with a 0.4 μm polycarbonate filter. We took 20 mL aliquots from the filtrate for each sample split for MRP and total P analyses, and kept them refrigerated until analysis within 24 h. We added 200 mL of ultrapure water to the solid residue for each sample split as a wash step after the above reductive step, shook samples for 2 h, and then centrifuged them at 3,700 rpm for 15 min. We filtered the supernatants with 0.4 μm polycarbonate filters and set aside 20 mL of filtrate from each sample split for MRP and total P analyses. We then extracted the solid sample residues in 200 mL of sodium acetate buffer (pH 4.0) for 6 h. At the end of this extraction step, we centrifuged the bottles at 3,700 rpm for 15 min, filtered the supernatants with 0.4 μm polycarbonate filters and took a 20 mL aliquot of filtrate from each sample split for MRP and total P analyses. We added 200 mL of ultrapure water to the solid residue for each sample split as a wash step, shook samples for 2 h, and then centrifuged them at 3,700 rpm for 15 min. We filtered the supernatants with 0.4 μm polycarbonate filters and set aside 20 mL of filtrate from each sample split for MRP and total P analyses. We repeated the water rinse step, and collected aliquots for MRP and total P analyses as in the previous steps. The concentrations of TP were determined as described below. Solid sediment sample residues following the pretreatment described above were transferred to two 50 mL centrifuge tubes (2 sample replicates combined per tube). We added 20 mL of 0.25 M NaOH + 0.05 M Na2EDTA solution to each tube, vortexed until all sediment was resuspended and then shook samples for 6 h at room temperature (Cade-Menun et al. 2005). We used a solid to solution ratio of 1:5 for this step to minimize the amount of freeze-dried material that will need to be dissolved for the 31P NMR experiments. Large amounts of salts from the NaOH-EDTA concentrated in NMR samples lead to higher viscosity and increase line broadening on NMR spectra (Cade-Menun and Liu 2014). We chose an extraction time of 6 h to improve total P recovery while limiting the degradation of natural P compounds in the sample. At the end of the extraction, samples were centrifuged at 3,700 rpm for 15 min and supernatants decanted into 50 mL centrifuge tubes. We collected a 500 μL aliquot from each sample, which we diluted with 4.5 mL of ultrapure water. These were refrigerated until analysis for total P content on the ICP-OES. The sample residues and supernatants were frozen on a slant to maximize the exposed surface area during the lyophilization step; this was done immediately after the removal of the 500 μL aliquot. Once completely frozen, the uncapped tubes containing supernatants and residues were freeze-dried over the course of 48 h. Each tube was covered with parafilm with small holes from a tack to minimize contamination. Freeze-dried supernatants from identical sample splits were combined and dissolved in 500 μL each of ultrapure water, D2O, NaOH-EDTA and 10 M NaOH prior to 31P NMR analysis. The D2O is required as signal lock in the spectrometer (Cade-Menun and Liu 2014). Sample pH was maintained at a pH &gt; 12 to optimize peak separation (Cade-Menun 2005; Cade-Menun and Liu 2014). Sample pH was assessed with a glass electrode, and verified with pH paper to account for the alkaline error caused by the high salt content of our samples (Covington 1985). Spectra were acquired immediately following sample preparation on a Varian Unity INOVA 600 MHz spectrometer equipped with a 10-mm broadband probe [operated by the Stanford Magnetic Resonance Laboratory at Stanford University]. We used a 10-mm rather than a 5-mm probe because larger tubes contain a greater concentration of P and thus require fewer scans to achieve similar signal to noise ratios (Cade-Menun and Liu 2014). The analytical parameters used were: 20oC, 90o pulse, 0.48 s acquisition time, 4.52 s delay time, 5600 scans (8 h experiments), no spin and an external H3PO4 standard. We maintained samples at a temperature of 20oC during experiments to achieve optimal spectral resolution (Crouse et al. 2000) and to minimize sample degradation. No proton decoupling was used out of concern for sample degradation (Cade-Menun and Liu 2014). The ratio of P to Fe and manganese (Mn) was used as a proxy for spin-lattice relaxation times (T1) to ensure adequate delays between pulses and thus quantitative spectra (McDowell et al. 2006). We used 5 s recycle delays, which correspond to three to five times the calculated T1 values, as recommended by McDowell et al. (2006). Peak identification was based on literature (Turner et al. 2003; Cade-Menun 2015). 31P NMR data were processed using the NMR Utility Transform software (NUTS, Acorn NMR). Peak areas were calculated by integration of spectra processed with a 7 Hz line broadening following baseline correction, peak picking and phasing. We accepted peaks that (1) represented at least 1% of the tallest peak in the total integrated area, (2) were identified by the NUTS software and (3) were confirmed as signal by visual inspection. Freeze-dried sample residues were ashed in crucibles at 550°C for 2 h and then extracted in 25 mL of 0.5 M sulfuric acid for 16 h (Olsen and Sommers 1982; Cade-Menun and Lavkulich 1997). We centrifuged samples at 3,700 rpm for 15 min, filtered supernatants with 0.4 μm polycarbonate filters, and measured P content on an ICP-OES. Total P concentrations in sediment extracts were measured using inductively coupled plasma optical emission spectroscopy (ICP-OES). Standards were prepared with the same solutions as those used for the extraction procedure in order to minimize matrix effects on P measurements. Sediment extracts and standards (0 μM, 3.2 μM, 32 μM and 320 μM) were diluted to lower salt content to prevent salt buildup on the nebulizer (1:20 dilution for step 1, 1:10 for steps 2 4). Concentration data from both wavelengths (213 nm and 214 nm) were averaged to obtain extract concentrations for each sample. The detection limit for P on this instrument for both wavelengths is 0.4 μM. The MRP concentrations were measured on a QuikChem 8000 automated ion analyzer. Standards were prepared with the same solutions used for the extraction step to minimize matrix effects on P measurements. Sediment extracts and standards (0 30 μM PO4) were diluted ten-fold to prevent matrix interference with color development. The detection limit for P on this instrument is 0.2 μM. We derived MUP concentrations by subtracting MRP from total P concentrations. from Cruise: TT013 <p><span style="font-size:13px">TT013-06MC</span></p> Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact QuikChem 8000 automated ion analyzer QuikChem 8000 automated ion analyzer PI Supplied Instrument Name: QuikChem 8000 automated ion analyzer Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/ Cruise: P194AR P194AR Community Standard Description NERC Vocabulary Server USCGC Polar Sea vessel Cruise: W9807A W9807A R/V Wecoma Community Standard Description NERC Vocabulary Server R/V Wecoma vessel W9807A Homa Lee United States Geological Survey Cruise: TT013 TT013 R/V Thomas G. Thompson Community Standard Description International Council for the Exploration of the Sea R/V Thomas G. Thompson vessel TT013 Margaret Leinen University of Rhode Island Community Standard Description NERC Vocabulary Server USCGC Polar Sea vessel R/V Wecoma Community Standard Description NERC Vocabulary Server R/V Wecoma vessel R/V Thomas G. Thompson Community Standard Description International Council for the Exploration of the Sea R/V Thomas G. Thompson vessel