http://lod.bco-dmo.org/id/dataset/805206 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2020-03-02 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Total molybdate reactive and unreactive phosphorus concentrations from sediment extracts from sediment samples collected during cruises in the Arctic Ocean, California Margin, and Equatorial Pacific from 1992-1998 2020-06-23 publication 2020-06-23 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2020-07-02 publication https://doi.org/10.26008/1912/bco-dmo.805206.1 Adina Paytan University of California-Santa Cruz principalInvestigator Dr Delphine Defforey University of California-Santa Cruz principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Paytan, A., Defforey, D. (2020) Total molybdate reactive and unreactive phosphorus concentrations from sediment extracts from sediment samples collected during cruises in the Arctic Ocean, California Margin, and Equatorial Pacific from 1992-1998. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-06-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.805206.1 [access date] Dataset Description: Total molybdate reactive and unreactive phosphorus concentrations from sediment extracts from sediment samples collected during cruises in the Arctic Ocean, California Margin, and Equatorial Pacific from 1992-1998. These data were published in Defforey et al. (2017). See the related-resource page https://www.bco-dmo.org/related-resource/794727 for other datasets related to this publication. Sediment sample information for this dataset is available as a supplemental document (Sediment_Sample_Info.csv) which contains collection date, water depth, sediment depth, latitude, and longitude. Additional award information: * NSF C-DEBI subaward # 156246 to Adina Paytan * NSF C-DEBI subaward # 157598 to Delphine Defforey Methods and Sampling: Locations: Arctic Ocean: P-1-94-AR P21, 84°5' N, 174°58' W California margin: W-2-98-NC TF1, 41°5' N, 125°1' W Equatorial Pacific: TT013-06MC, 12°00' S, 134°56' W Methodology: We used the ignition method to determine total P and molybdate-reactive P concentrations (MRP, which includes primarily free orthophosphate) for each sediment sample used for this study. Samples for total P analyses were ashed in crucibles at 550oC for 2 h and then extracted in 25 mL of 0.5 M sulfuric acid for 16 h. Samples for MRP analyses were extracted in the same manner, without the ashing step (Olsen and Sommers 1982; Cade-Menun and Lavkulich 1997). We derived molybdate-unreactive P concentrations (MUP, which includes primarily organic P and polyphosphates) in supernatants by subtracting MRP from total P concentrations. For ashed and unashed extracts, MRP was determined as described below. Total P concentrations in sediment extracts were measured using inductively coupled plasma optical emission spectroscopy (ICP-OES). Standards were prepared with the same solutions as those used for the extraction procedure in order to minimize matrix effects on P measurements. Sediment extracts and standards (0 μM, 3.2 μM, 32 μM and 320 μM) were diluted to lower salt content to prevent salt buildup on the nebulizer. Concentration data from both wavelengths (213 nm and 214 nm) were averaged to obtain extract concentrations for each sample. The detection limit for P on this instrument for both wavelengths is 0.4 μM. The MRP concentrations were measured on a QuikChem 8000 automated ion analyzer. Standards were prepared with the same solutions used for the extraction step to minimize matrix effects on P measurements. Sediment extracts and standards (0 – 30 μM PO4) were diluted ten-fold to prevent matrix interference with color development. The detection limit for P on this instrument is 0.2 μM. We derived MUP concentrations by subtracting MRP from total P concentrations. Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0939564 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0939564 completed Adina Paytan University of California-Santa Cruz 831-459-1437 Earth & Marine Sciences C308 1156 High St Santa Cruz CA 95064 USA apaytan@ucsc.edu pointOfContact Dr Delphine Defforey University of California-Santa Cruz Department of Earth and Planetary Sciences 1156 High Street Santa Cruz CA 95060 USA ddeffore@ucsc.edu pointOfContact asNeeded Dataset Version: 1 Unknown Replicate Sample_Name Peak_Concentration Actual_PO4 umol_PO4 Sediment_mass umol_PO4_per_g QuikChem 8000 automated ion analyzer theme None, User defined replicate sample identification reactive phosphorus (PO4) Phosphorus weight featureType BCO-DMO Standard Parameters Flow Injection Analyzer instrument BCO-DMO Standard Instruments P194AR W9807A TT013 service Deployment Activity Arctic Ocean Eureka, California, Northern California U.S. JGOFS Equatorial Pacific place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Center for Dark Energy Biosphere Investigations http://www.darkenergybiosphere.org Center for Dark Energy Biosphere Investigations The mission of the Center for Dark Energy Biosphere Investigations (C-DEBI) is to explore life beneath the seafloor and make transformative discoveries that advance science, benefit society, and inspire people of all ages and origins. C-DEBI provides a framework for a large, multi-disciplinary group of scientists to pursue fundamental questions about life deep in the sub-surface environment of Earth. The fundamental science questions of C-DEBI involve exploration and discovery, uncovering the processes that constrain the sub-surface biosphere below the oceans, and implications to the Earth system. What type of life exists in this deep biosphere, how much, and how is it distributed and dispersed? What are the physical-chemical conditions that promote or limit life? What are the important oxidation-reduction processes and are they unique or important to humankind? How does this biosphere influence global energy and material cycles, particularly the carbon cycle? Finally, can we discern how such life evolved in geological settings beneath the ocean floor, and how this might relate to ideas about the origin of life on our planet? C-DEBI's scientific goals are pursued with a combination of approaches: (1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites; (2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems; (3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and (4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists. Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI. Data Management: C-DEBI is committed to ensuring all the data generated are publically available and deposited in a data repository for long-term storage as stated in their Data Management Plan (PDF) and in compliance with the NSF Ocean Sciences Sample and Data Policy. The data types and products resulting from C-DEBI-supported research include a wide variety of geophysical, geological, geochemical, and biological information, in addition to education and outreach materials, technical documents, and samples. All data and information generated by C-DEBI-supported research projects are required to be made publically available either following publication of research results or within two (2) years of data generation. To ensure preservation and dissemination of the diverse data-types generated, C-DEBI researchers are working with BCO-DMO Data Managers make data publicly available online. The partnership with BCO-DMO helps ensure that the C-DEBI data are discoverable and available for reuse. Some C-DEBI data is better served by specialized repositories (NCBI's GenBank for sequence data, for example) and, in those cases, BCO-DMO provides dataset documentation (metadata) that includes links to those external repositories. C-DEBI largerWorkCitation program A new marine sediment sample preparation scheme for solution 31P NMR analysis https://osprey.bco-dmo.org/project/664054 A new marine sediment sample preparation scheme for solution 31P NMR analysis <p>We developed and tested a new approach to prepare marine sediment samples for solution 31P nuclear magnetic resonance spectroscopy (31P NMR). This approach addresses the effects of sample pretreatment on sedimentary P composition and increases the signal of low abundance P species in 31P NMR spectra by removing up the majority inorganic P  from sediment samples while causing minimal alteration of the chemical structure of organic P compounds. The method was tested on natural marine sediment samples from different localities (Equatorial Pacific, California Margin and Arctic Ocean) with high inorganic P content, and allowed for the detection of low abundance P forms in samples for which only an orthophosphate signal could be resolved with an NaOH-EDTA extraction alone. This new approach will allow the use of 31P NMR on samples for which low organic P concentrations previously hindered the use of this tool, and will help answer longstanding question regarding the fate of organic P in marine sediments. We developed and tested a new approach to prepare marine sediment samples for solution 31P nuclear magnetic resonance spectroscopy (31P NMR). This approach addresses the effects of sample pretreatment on sedimentary P composition and increases the signal of low abundance P species in 31P NMR spectra by removing up the majority inorganic P  from sediment samples while causing minimal alteration of the chemical structure of organic P compounds. The method was tested on natural marine sediment samples from different localities (Equatorial Pacific, California Margin and Arctic Ocean) with high inorganic P content, and allowed for the detection of low abundance P forms in samples for which only an orthophosphate signal could be resolved with an NaOH-EDTA extraction alone. This new approach will allow the use of 31P NMR on samples for which low organic P concentrations previously hindered the use of this tool, and will help answer longstanding question regarding the fate of organic P in marine sediments. </p> <p>NSF C-DEBI Award #156246 to Dr. Adina Paytan</p> <p>NSF C-DEBI Award #157598 to Dr. Delphine Defforey </p> Marine Sediment Analysis 31P NMR largerWorkCitation project eng; USA oceans Arctic Ocean; Eureka, California, Northern California; U.S. JGOFS Equatorial Pacific -174.967 -125.017 -12 84.083 1992-10-30 1998-07-24 Equatorial Pacific, California Margin, Arctic Ocean 0 BCO-DMO catalogue of parameters from Total molybdate reactive and unreactive phosphorus concentrations from sediment extracts from sediment samples collected during cruises in the Arctic Ocean, California Margin, and Equatorial Pacific from 1992-1998 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/809929.rdf Name: Replicate Units: unitless Description: <p>Replicate of an individual sample</p> http://lod.bco-dmo.org/id/dataset-parameter/809930.rdf Name: Sample_Name Units: unitless Description: <p>Sample name</p> http://lod.bco-dmo.org/id/dataset-parameter/809931.rdf Name: Peak_Concentration Units: micromolar (uM) Description: <p>Phosphate concentration (uncorrected)</p> http://lod.bco-dmo.org/id/dataset-parameter/809932.rdf Name: Actual_PO4 Units: micromolar (uM) Description: <p>Phosphate concentration corrected for dilution</p> http://lod.bco-dmo.org/id/dataset-parameter/809933.rdf Name: umol_PO4 Units: micromoles (umol) Description: <p>Amount of phosphorus extracted</p> http://lod.bco-dmo.org/id/dataset-parameter/809934.rdf Name: Sediment_mass Units: grams (g) Description: <p>Dried sediment mass</p> http://lod.bco-dmo.org/id/dataset-parameter/809935.rdf Name: umol_PO4_per_g Units: micromoles per gram (umol/g) Description: <p>Micromoles of phosphorus per gram of sediment (ground dry weight)</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2444 https://darchive.mblwhoilibrary.org/bitstream/1912/25936/1/dataset-805206_sediment-tp-mrp-mup__v1.tsv download https://doi.org/10.26008/1912/bco-dmo.805206.1 download onLine dataset Locations: Arctic Ocean: P-1-94-AR P21, 84°5' N, 174°58' W California margin: W-2-98-NC TF1, 41°5' N, 125°1' W Equatorial Pacific: TT013-06MC, 12°00' S, 134°56' W Methodology: We used the ignition method to determine total P and molybdate-reactive P concentrations (MRP, which includes primarily free orthophosphate) for each sediment sample used for this study. Samples for total P analyses were ashed in crucibles at 550oC for 2 h and then extracted in 25 mL of 0.5 M sulfuric acid for 16 h. Samples for MRP analyses were extracted in the same manner, without the ashing step (Olsen and Sommers 1982; Cade-Menun and Lavkulich 1997). We derived molybdate-unreactive P concentrations (MUP, which includes primarily organic P and polyphosphates) in supernatants by subtracting MRP from total P concentrations. For ashed and unashed extracts, MRP was determined as described below. Total P concentrations in sediment extracts were measured using inductively coupled plasma optical emission spectroscopy (ICP-OES). Standards were prepared with the same solutions as those used for the extraction procedure in order to minimize matrix effects on P measurements. Sediment extracts and standards (0 μM, 3.2 μM, 32 μM and 320 μM) were diluted to lower salt content to prevent salt buildup on the nebulizer. Concentration data from both wavelengths (213 nm and 214 nm) were averaged to obtain extract concentrations for each sample. The detection limit for P on this instrument for both wavelengths is 0.4 μM. The MRP concentrations were measured on a QuikChem 8000 automated ion analyzer. Standards were prepared with the same solutions used for the extraction step to minimize matrix effects on P measurements. Sediment extracts and standards (0 – 30 μM PO4) were diluted ten-fold to prevent matrix interference with color development. The detection limit for P on this instrument is 0.2 μM. We derived MUP concentrations by subtracting MRP from total P concentrations. from Cruise: TT013 <p><span style="font-size:13px">TT013-06MC</span></p> Specified by the Principal Investigator(s) Data were processed in Excel. BCO-DMO Data Manager Processing Notes: * Data from originally submitted Excel file Data_sediment_TP_MRP_MUP_v2.xlsx exported as csv. * added a conventional header with dataset name, PI name, version date * modified parameter names to conform with BCO-DMO naming conventions * blank values in this dataset are displayed as &quot;nd&quot; for &quot;no data.&quot; nd is the default missing data identifier in the BCO-DMO system. Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact QuikChem 8000 automated ion analyzer QuikChem 8000 automated ion analyzer PI Supplied Instrument Name: QuikChem 8000 automated ion analyzer Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/ Cruise: P194AR P194AR Community Standard Description NERC Vocabulary Server USCGC Polar Sea vessel Cruise: W9807A W9807A R/V Wecoma Community Standard Description NERC Vocabulary Server R/V Wecoma vessel W9807A Homa Lee United States Geological Survey Cruise: TT013 TT013 R/V Thomas G. Thompson Community Standard Description International Council for the Exploration of the Sea R/V Thomas G. Thompson vessel TT013 Margaret Leinen University of Rhode Island Community Standard Description NERC Vocabulary Server USCGC Polar Sea vessel R/V Wecoma Community Standard Description NERC Vocabulary Server R/V Wecoma vessel R/V Thomas G. Thompson Community Standard Description International Council for the Exploration of the Sea R/V Thomas G. Thompson vessel