http://lod.bco-dmo.org/id/dataset/805612
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-03-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Environmental observations and infected host and dinospore counts in Salt Pond, Falmouth, Massachusetts, USA from 2018 through 2019
2020-03-10
publication
2020-03-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-03-20
publication
https://doi.org/10.26008/1912/bco-dmo.805612.1
Rebecca J. Gast
Woods Hole Oceanographic Institution
principalInvestigator
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
principalInvestigator
Maria G. Pachiadaki
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gast, R. J., Edgcomb, V. P., Pachiadaki, M. G. (2023) Environmental observations and infected host and dinospore counts in Salt Pond, Falmouth, Massachusetts, USA from 2018 through 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-03-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.805612.1 [access date]
Site and sampling information, infected host and dinospore count data. Dataset Description: <p>Salt Pond is a shallow, seasonally stratified coastal salt pond located in Falmouth, MA and it is proximal to the labs at Woods Hole Oceanographic Institution (WHOI). This estuarine system is tidally influenced by Vineyard Sound and receives inputs of freshwater primarily from groundwater. Seasonal abiotic factors and nutrient availability in the seasonally stratified water column influence the growth and abundance of diverse microbial taxa within this system, providing an ideal setting to study syndinian parasites and host diversity temporally and across oxygen gradients. Salt Pond has anoxic waters that come within 2-3 meters of the water surface in summer and bottom waters that become increasingly sulfidic due to bacterial sulfate reduction as seasonal stratification intensifies.</p> Methods and Sampling: <p>For the field sampling protocol, please see the attached Supplemental File&nbsp;<a href="https://datadocs.bco-dmo.org/docs/302/Coastal_Parasites/data_docs/805612/1/FieldSamplingProtocol_2018_BCO-DMO.docx" target="_blank">FieldSamplingProtocol_2018_BCO-DMO.docx</a>.</p>
<p>For information concerning the in-situ hybridization, please see the Supplemental Files&nbsp;<a href="https://datadocs.bco-dmo.org/docs/302/Coastal_Parasites/data_docs/805612/1/CARD-FISH_Protocol.pdf" target="_blank">CARD-FISH Protocol.pdf</a> and <a href="https://datadocs.bco-dmo.org/docs/302/Coastal_Parasites/data_docs/805612/1/Reagents_for_CARD-FISH.pdf" target="_blank">Reagents for CARD-FISH .pdf</a>.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851012 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851012
completed
Rebecca J. Gast
Woods Hole Oceanographic Institution
508-289-3209
266 Woods Hole Rd. MS #32
Woods Hole
MA
02543
USA
rgast@whoi.edu
pointOfContact
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
508-289-3734
Department of Geology and Geophysics 220 McLean Lab, MS #8
Woods Hole
MA
02543
USA
vedgcomb@whoi.edu
pointOfContact
Maria G. Pachiadaki
Woods Hole Oceanographic Institution
508 289 2943
Redfield Lab
Woods Hole
MA
02543
mpachiadaki@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
Sampling_Date
Depth
Month
Temperature
Salinity
Dissolved_Oxygen
NH4
NO2_NO3
Moon_Phase_Illumination
Day_Length
Avg_wind
Max_Gust
Wind_Direction
Tide
Tide_text
Vol_Filtered_mL
Infection_Count
No_of_grids_counted
No_of_cells
Total_euk_count
Percent_infected
Cast
No_Spores
Vol_Filtered
spores_per_L
lat
lon
Go-Flo bottle
YSI datasonde
theme
None, User defined
sample identification
date
depth
month of year
water temperature at measurement depth
salinity
dissolved Oxygen
Ammonium
nitrate plus nitrite
No BCO-DMO term
length_day
wind_speed
wind_dir
tide
volume of water filtered
count
abundance
cast
latitude
longitude
featureType
BCO-DMO Standard Parameters
GO-FLO Bottle
YSI Sonde 6-Series
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Trojan Horses in the Marine Realm: Protist Parasite-host Dynamics in Coastal Waters
https://osprey.bco-dmo.org/project/786261
Trojan Horses in the Marine Realm: Protist Parasite-host Dynamics in Coastal Waters
<p><em>NSF Award Abstract:</em><br />
The ecological importance of parasitic dinoflagellates has been recognized for some time, particularly during epidemic outbreaks that cause mass mortality of their hosts, damage to aquaculture, and render commercially valuable Crustacea unpalatable. The dominate parasitic dinoflagellate group found in international global ocean surveys is referred to as MALV II Syndiniales. In the planktonic environment, the MALV II Syndiniales group not only exerts top-down controls on their prey populations, but based on their apparent ubiquity and abundance, they likely shape the pools of nutrients in marine water columns. Data on cultured samples reveals this hyper-diverse group can infect a wide range of protist hosts, as well as copepods, and fish larvae. Gaps in knowledge of the specificity and dynamics of the host-parasite interactions contribute to difficulties in estimating the impacts on the coastal ecosystems. In this project, researchers combine novel methods in microscopy, genomics, and chemistry to track host-parasite dynamics at a coastal site over an annual cycle followed by modeling to assess the impacts on microbial ecosystem dynamics. The researchers will engage undergraduate and high school students in field and laboratory research activities. In addition, support for a graduate student is included along with plans to disseminate the research results more broadly through publications and presentations.</p>
<p>Syndiniales parasitism is a widespread, albeit under-studied symbiotic interaction in the marine environment and little is known about regulation of protist populations by these parasites. In spite of their cosmopolitan distribution in the global ocean and their apparent abundance in molecular datasets of protist marker genes, little is known about the ecology of these parasites and almost no genomic data exists for them. In this project, the researchers combine high-resolution sampling, water chemistry (including nutrients) analyses, molecular marker gene analyses, fluorescence in situ hybridization, single cell genomics, and modeling to produce the first focused assessment of host-MALVII parasite dynamics and ecology at the community level in a coastal marine ecosystem. The researchers will evaluate temporal dynamics of host and parasite diversity and will examine temporal variation in levels of infection of the protist community and host-parasite specificity using high-resolution sampling in Salt Pond, Falmouth, MA, and in situ hybridization microscopy. Molecular approaches include amplicon tag high throughput sequencing, leveraging the emerging third generation sequencing technology, Oxford Nanopore's MinION to elucidate host-parasite identities. The researchers will also apply advances in single-cell genome sequencing to inform on strain-specific genome content, including the molecular mechanisms underpinning protist parasitism. Contributions to pools of particulate and dissolved organic matter will be estimated for several of the most commonly infected host taxa in Salt Pond using laboratory experiments, providing the first set of values for modeling impacts of Syndiniales parasitism on pools of organic and inorganic nutrients.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Coastal Parasites
largerWorkCitation
project
eng; USA
oceans
-70.62723
-70.62723
41.54482
41.54482
2018-03-19
2019-08-09
Salt Pond, Falmouth, MA
0
BCO-DMO catalogue of parameters from Environmental observations and infected host and dinospore counts in Salt Pond, Falmouth, Massachusetts, USA from 2018 through 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/805620.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample_ID C = cast (replicates 1 and 2) D = depth (1=2m; 2=3m; 3=4m)</p>
http://lod.bco-dmo.org/id/dataset-parameter/805621.rdf
Name: Sampling_Date
Units: unitless
Description: <p>date of sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/805622.rdf
Name: Depth
Units: meters (m)
Description: <p>depth of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/805623.rdf
Name: Month
Units: unitless
Description: <p>Month of sample as text</p>
http://lod.bco-dmo.org/id/dataset-parameter/805624.rdf
Name: Temperature
Units: degrees Celsius
Description: <p>Water temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/805625.rdf
Name: Salinity
Units: parts per thousand (ppt)
Description: <p>Water salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/805626.rdf
Name: Dissolved_Oxygen
Units: miligrams per liter (mg/L)
Description: <p>Dissolved Oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/805627.rdf
Name: NH4
Units: micromolar
Description: <p>NH4</p>
http://lod.bco-dmo.org/id/dataset-parameter/805628.rdf
Name: NO2_NO3
Units: micromolar
Description: <p>NO2_NO3</p>
http://lod.bco-dmo.org/id/dataset-parameter/805629.rdf
Name: Moon_Phase_Illumination
Units: percent illumination
Description: <p>Moon phase</p>
http://lod.bco-dmo.org/id/dataset-parameter/805630.rdf
Name: Day_Length
Units: decimal hours
Description: <p>length of day as hours:minutes converted to a decimal</p>
http://lod.bco-dmo.org/id/dataset-parameter/805631.rdf
Name: Avg_wind
Units: miles per hour (mph)
Description: <p>Average wind speed measured at the Coast Guard Station at Woods Hole</p>
http://lod.bco-dmo.org/id/dataset-parameter/805632.rdf
Name: Max_Gust
Units: miles per hour (mph)
Description: <p>Maximum wind gust speed measured at the Coast Guard Station at Woods Hole</p>
http://lod.bco-dmo.org/id/dataset-parameter/805633.rdf
Name: Wind_Direction
Units: degrees
Description: <p>Direction of wind as compass direction converted to degrees</p>
http://lod.bco-dmo.org/id/dataset-parameter/805634.rdf
Name: Tide
Units: unitless
Description: <p>tide level at sampling (low=1;slack=2;high=3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/805635.rdf
Name: Tide_text
Units: unitless
Description: <p>Textual representation of tide level</p>
http://lod.bco-dmo.org/id/dataset-parameter/805636.rdf
Name: Vol_Filtered_mL
Units: milliliter (mL)
Description: <p>amount of water filteres</p>
http://lod.bco-dmo.org/id/dataset-parameter/805637.rdf
Name: Infection_Count
Units: count
Description: <p>number of cells exhibiting infection by CARD-FISH</p>
http://lod.bco-dmo.org/id/dataset-parameter/805638.rdf
Name: No_of_grids_counted
Units: count
Description: <p>number of grids counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/805639.rdf
Name: No_of_cells
Units: count
Description: <p>total number of cells counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/805640.rdf
Name: Total_euk_count
Units: unknown
Description: <p># of cells divided by # of grids counted then multiplied by microscope correction factor 7693</p>
http://lod.bco-dmo.org/id/dataset-parameter/805641.rdf
Name: Percent_infected
Units: unitless (percent)
Description: <p>Infection_Count divided by Total_euk_count</p>
http://lod.bco-dmo.org/id/dataset-parameter/805642.rdf
Name: Cast
Units: unitless
Description: <p>replicates (1 or 2)</p>
http://lod.bco-dmo.org/id/dataset-parameter/805643.rdf
Name: No_Spores
Units: count
Description: <p>dinospores counted by CARD-FISH</p>
http://lod.bco-dmo.org/id/dataset-parameter/805644.rdf
Name: Vol_Filtered
Units: milliliter (mL)
Description: <p>amount of water filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/805645.rdf
Name: spores_per_L
Units: count per liter (count/L)
Description: <p>spore count multiplied by 8; multiplied by 50</p>
http://lod.bco-dmo.org/id/dataset-parameter/805646.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude of sampling location with positive values indicating North</p>
http://lod.bco-dmo.org/id/dataset-parameter/805647.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude of sampling location with negative values indicating West</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
51031
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/91a735ca-ccf9-4109-84fb-b68baca2f136/content
download
https://doi.org/10.26008/1912/bco-dmo.805612.1
download
onLine
dataset
<p>For the field sampling protocol, please see the attached Supplemental File&nbsp;<a href="https://datadocs.bco-dmo.org/docs/302/Coastal_Parasites/data_docs/805612/1/FieldSamplingProtocol_2018_BCO-DMO.docx" target="_blank">FieldSamplingProtocol_2018_BCO-DMO.docx</a>.</p>
<p>For information concerning the in-situ hybridization, please see the Supplemental Files&nbsp;<a href="https://datadocs.bco-dmo.org/docs/302/Coastal_Parasites/data_docs/805612/1/CARD-FISH_Protocol.pdf" target="_blank">CARD-FISH Protocol.pdf</a> and <a href="https://datadocs.bco-dmo.org/docs/302/Coastal_Parasites/data_docs/805612/1/Reagents_for_CARD-FISH.pdf" target="_blank">Reagents for CARD-FISH .pdf</a>.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
-&nbsp;added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- added lat and&nbsp;lon&nbsp;fields<br />
- reformatted&nbsp;date to yyyy-mm-dd<br />
-&nbsp;&nbsp;joined environmental, infection, and spore count datasets on the sample date, cast number, and depth fields.</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Go-Flo bottle
Go-Flo bottle
PI Supplied Instrument Name: Go-Flo bottle PI Supplied Instrument Description:~2L Go-Flo bottle for sampling water Instrument Name: GO-FLO Bottle Instrument Short Name:GO-FLO Instrument Description: GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/
YSI datasonde
YSI datasonde
PI Supplied Instrument Name: YSI datasonde PI Supplied Instrument Description:YSI datasonde to record temperature, salinity and oxygen Instrument Name: YSI Sonde 6-Series Instrument Short Name:YSI Sonde 6-Series Instrument Description: YSI 6-Series water quality sondes and sensors are instruments for environmental monitoring and long-term deployments. YSI datasondes accept multiple water quality sensors (i.e., they are multiparameter sondes). Sondes can measure temperature, conductivity, dissolved oxygen, depth, turbidity, and other water quality parameters. The 6-Series includes several models. More from YSI. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0737/