http://lod.bco-dmo.org/id/dataset/805653
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-03-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Planktic foraminifer tissue and shell d15N from net tows on R/V S.A. Agulhas II cruises VOY016 and VOY019 in the Southern Ocean south of Africa during 2015-2016
2020-03-10
publication
2020-03-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-03-13
publication
https://doi.org/10.1575/1912/bco-dmo.805653.1
Daniel M. Sigman
Princeton University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sigman, D. (2020) Planktic foraminifer tissue and shell d15N from net tows on R/V S.A. Agulhas II cruises VOY016 and VOY019 in the Southern Ocean south of Africa during 2015-2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-03-10 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.805653.1 [access date]
Planktic foraminifer tissue and shell d15N from net tows in the African sector Dataset Description: <p>This dataset synthesizes net-tow data (foraminifera and zooplankton measurements) with tow-depth seawater measurements (physical and chemical properties) and surface measurements of bulk suspended particulate organic nitrogen (PON) collected from the underway intake (7 m depth).</p> Methods and Sampling: <p>Planktic foraminifera were collected by towing a 200-μm-mesh plankton net for 90 min at a target depth within the upper mixed layer. Approximately 90% of the tow material was preserved in a 5-10% pH-buffered formalin solution and stored at 4°C until sorting for foraminifera (Ren et al., 2012). The remaining 10% was size fractionated and frozen at −20°C for element and isotope analysis of zooplankton. During each tow, bulk (&gt;0.3 µm) suspended PON and nitrate samples were collected from the underway intake (at 7 m depth) and frozen at −80°C and −20°C, respectively, until isotope analysis. <em>In situ</em> (i.e., tow-depth) nitrate samples and auxiliary hydrographic data come from CTD casts performed on station before the start of each net tow.</p>
<p>Foraminifera were separated by species, rinsed briefly with Milli-Q and crushed open to expose (non-shell-bound) tissue. Tissue N was converted to nitrate using the persulfate oxidation method (Nydahl, 1978; Knapp et al., 2005). Remnant shell material (having been oxidatively cleaned) was transferred, rinsed six times with Milli-Q and oven dried at 50°C. Shell samples were then dissolved with hydrochloric acid to release shell-bound organics for persulfate oxidation (Nydahl, 1978; Knapp et al., 2005). For both tissue- and shell-derived nitrate (from persulfate oxidation) as well as seawater nitrate, samples were measured for concentration by chemiluminescence (Braman &amp; Hendrix, 1989) and N isotope composition using the denitrifier method and gas chromatography−isotope ratio mass spectrometry (GC-IRMS) (Sigman et al., 2001; Casciotti et al., 2002; Weigand et al., 2016). The N isotope composition of bulk PON and size-fractionated zooplankton were measured by elemental analyser-IRMS.</p>
<p>The isotopic composition of N₂O was measured either at Princeton University (USA) or the Max Planck Institute for Chemistry (MPIC; Germany) by GC-IRMS using a Thermo MAT 253 mass spectrometer with a purpose-built on-line N₂O extraction and purification system. Bulk PON and size-fractionated zooplankton samples were measured at the University of Cape Town using a Thermo Scientific FLASH 2000 elemental analyzer coupled to a Thermo Scientific Delta V Plus mass spectrometer. Hydrographic data were acquired by a Sea-Bird conductivity-temperature depth sensor during each cast.</p>
<p>See methods section of Smart et al. (2020) for more detail.</p>
Funding provided by NSF Division of Polar Programs (NSF PLR) Award Number: PLR-1401489 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1401489
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1060947 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1060947
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0922345 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0922345
completed
Daniel M. Sigman
Princeton University
609-258-2194
Department of Geosciences M52 Guyot Hall
Princeton
NJ
08544
USA
sigman@princeton.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
cruise
ship
tow
date
time_starttow
time_endtow
species
type
analysis_year
latitude
longitude
depth_tow
d15N_foram_avg
d15N_foram_sd
d15N_foram_n
indiv_avg
indiv_sd
indiv_n
Ni_avg
Ni_sd
Ni_n
Nw_avg
Nw_sd
Nw_n
d15N_avg_nitrate
conc_avg_nitrate
d15N_avg_nitrate_nitrite
conc_avg_nitrate_nitrite
temperature_ctd
salinity_ctd
oxygen_ctd
fluorescence_ctd
potdens_calc
MLD_calc
conc_PON_avg
conc_PON_sd
conc_PON_n
d15N_PON_avg
d15N_PON_sd
d15N_PON_n
d15N_zoop_avg
d15N_zoop_se
d15N_zoop_n
ISO_DateTime_UTC_starttow
ISO_DateTime_UTC_endtow
Sea-Bird conductivity-temperature depth sensor
Thermo MAT 253 mass spectrometer
Thermo Scientific Delta V Plus
200-um-mesh plankton net
Thermo Scientific FLASH 2000
theme
None, User defined
cruise id
ship
tow
date
time of day
taxon_code
sample description
year
latitude
longitude
depth
d15N measured in biota
number of replicates
Nitrogen
dN15_NO3
Nitrate
delta 15N NO3+NO2
nitrate plus nitrite
water temperature
salinity
dissolved Oxygen
fluorescence
density
mixed layer depth
particulate organic nitrogen
d15N
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
Plankton Net
Elemental Analyzer
instrument
BCO-DMO Standard Instruments
VOY016
VOY019
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
High-resolution, Assemblage-specific Records of Diatom-bound N Isotopes from the Indian Sector of the Antarctic Ocean
https://www.bco-dmo.org/project/557250
High-resolution, Assemblage-specific Records of Diatom-bound N Isotopes from the Indian Sector of the Antarctic Ocean
<p><em>Description from NSF award abstract:</em><br />
The high concentration of the major nutrients nitrate and phosphate is a fundamental characteristic of the Antarctic Zone in the Southern Ocean and is central to its role in global ocean fertility and the global carbon cycle. The isotopic composition of diatom-bound organic nitrogen is one of the best hopes for reconstructing the nutrient status of polar surface waters over glacial cycles, which in turn may hold the explanation for the decline in atmospheric carbon dioxide during ice ages. The PIs propose to generate detailed diatom-bound nitrogen isotope (δ15Ndb) records from high sedimentation rate cores from the Kerguelen Plateau. Because the cores were collected at relatively shallow seafloor depths, they have adequate planktonic and benthic foraminifera to develop accurate age models. The resulting data could be compared with climate records from Antarctic ice cores and other archives to investigate climate-related changes, including the major steps into and out of ice ages and the millennial-scale events that occur during ice ages and at their ends. The records generated in this project will provide a critical test of hypotheses for the cause of lower ice age CO2.</p>
<p>This study will contribute to the goal of understanding ice ages and past CO2 changes, which both have broad implications for future climate. Undergraduates will undertake summer internships, with the possibility of extending their work into junior year projects and senior theses. In addition, the PI will lead modules for two Princeton programs for middle school teachers and will host a teacher for a six-week summer research project.</p>
Diatom-bound_N_Isotopes
largerWorkCitation
project
Understanding the nitrogen isotopes of planktonic foraminifera: A modern Sargasso Sea study
https://www.bco-dmo.org/project/743583
Understanding the nitrogen isotopes of planktonic foraminifera: A modern Sargasso Sea study
<p><em>NSF Award Abstract:</em><br />
Nitrogen (N) and phosphorus are the two nutrients required in large quantity by phytoplankton in the ocean, and together they limit productivity throughout most of the tropical, subtropical, and temperate ocean. Both the cycling of N and its input/output budget have been argued to control the fertility of the ocean and the ocean's role in setting atmospheric CO2. The CaCO3 tests of foraminifera can represent a substantial fraction of marine sediments and have been used extensively in paleoceanography; they are an obvious target for isotopic analysis of microfossil-bound organic matter.</p>
<p>In recent years, researchers at Princeton have developed a protocol for the isotopic analysis of foraminiferal shell-bound N. The current protocol is at least 100 times more sensitive than typical on-line combustion, allowing for rapid progress with a N isotope archive that was previously not feasible to measure. Measurements on surface sediments and a downcore record from the Caribbean show the promise of foraminifera-bound del15N (fb-del15N) to provide both a robust N isotope archive for paleoceanography, and one with a unique potential of richness, given the existence of multiple foraminiferal species with different depth habitats and behaviors. Moreover, the finding from the Caribbean Sea record -- reduced N fixation in ice age Atlantic -- has changed the scientific conversation about the nature of the input/output budget of oceanic fixed N and its potential to change ocean fertility and atmospheric CO2. However, the controls on fb-del15N have not yet been adequately studied.</p>
<p>In this project, as a first major step in developing a foundation for the paleoceanographic application of fb-del15N, the same Princeton University team will study its genesis in the water column, transport to the seafloor, and early diagenesis. They will undertake this study in the Sargasso Sea south of Bermuda. This is one of the best studied regions of the ocean, in general and with respect to foraminifera, and a region that has been has been a focus of the N isotope research of the PI for the last decade and others previously. Moreover, its significant seasonality -- in physical oceanography, biogeochemistry, and foraminiferal species abundance -- will facilitate the effort to understand the controls on fb-del15N at a mechanistic level. The research team will participate in six Bermuda Atlantic Time-series Study (BATS) cruises over two years, collecting foraminifera and other N forms likely to provide insight into the controls on fb-del15N. From the nearby Oceanic Flux Program (OFP) moored sediment traps and from shallow sediments collected in the region, they will pick foraminifera shells and again make relevant ancillary measurements. This work will establish the relationship of foraminiferal biomass to shell-bound del15N for different species, and comparison of the foraminiferal isotope data with the upper ocean N pools will yield empirical isotopic relationships and work toward a mechanistic insight of fb-del15N (e.g., the importance of different N pools to the diets of different foraminifera; the role of algal symbionts). The sediment trap and surface sediment data will support the plankton tow data by integrating over longer time scales and will also address questions regarding late stage (e.g., gametogenic) calcification and the early diagenesis of fb-del15N and fb-N content.</p>
<p>Broader Impacts: This study will yield an improved understanding of the nutrient dynamics of foraminifera, a class of organisms whose shells are a central tool in micropaleontology and paleoclimatology. The project will also build on the principal investigator's involvement in the Bermuda Institute of Ocean Sciences as an asset for integrating ocean-related education and research at both the undergraduate and graduate levels.</p>
N Isotopes Foraminifera
largerWorkCitation
project
MRI: Acquisition of Stable Isotope Instrumentation for the Biogeosciences at Princeton University
https://www.bco-dmo.org/project/788982
MRI: Acquisition of Stable Isotope Instrumentation for the Biogeosciences at Princeton University
<p>NSF Award Abstract:</p>
<p>Intellectual Merit:<br />
The PIs are requesting funds to acquire a suite of instruments for stable isotope (N) research, including a denitrifier-based, natural abundance isotope system, a tracer isotope system, and general purpose natural abundance peripherals for an existing mass spectrometer. Specific instruments requested include a Thermo Electron MAT 253 stable isotope mass spectrometer, a Thermo DeltaVAdvantage stable isotope mass spectrometer with peripherals, an EA peripheral for combustion-based isotope analysis, and a GasBench II carbon dioxide preparation and purification system. The proposed instrumentation would enable research efforts examining natural stable isotope abundances that include ocean biogeochemistry and paleoceanography, terrestrial biogeochemistry, carbon isotopes in Precambrian Earth history. Stable isotope tracer research would include nitrification, nitrogen fixation, and carbon partitioning during biostimulation.</p>
<p>Broader Impacts:<br />
The PIs state that the proposed instrumentation would expand the research capabilities of the institution and specifically the Departments of Geosciences and Ecology and Evolutionary Biology. The improvements to the denitrifier method will also serve to expand the capabilities of the ocean research community at large. Additionally, the proposed instrumentation will enable method development and provide training for undergraduate students at the institution and those participating in a collaborative effort with the University of Cape Town.</p>
stable isotope instrumentation
largerWorkCitation
project
eng; USA
oceans
0.0483
38.4761
-53.9942
-40.9588
2015-07-27
2016-05-09
From projects that focused on the following 2 locations: 1. Kerguelen Plateau 2. Sargasso Sea
0
BCO-DMO catalogue of parameters from Planktic foraminifer tissue and shell d15N from net tows on R/V S.A. Agulhas II cruises VOY016 and VOY019 in the Southern Ocean south of Africa during 2015-2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/805672.rdf
Name: cruise
Units: unitless
Description: Cruise identifier
http://lod.bco-dmo.org/id/dataset-parameter/805673.rdf
Name: ship
Units: unitless
Description: Ship name
http://lod.bco-dmo.org/id/dataset-parameter/805674.rdf
Name: tow
Units: unitless
Description: Tow number
http://lod.bco-dmo.org/id/dataset-parameter/805675.rdf
Name: date
Units: unitless
Description: Date; format: yyyy-mm-dd
http://lod.bco-dmo.org/id/dataset-parameter/805676.rdf
Name: time_starttow
Units: unitless
Description: Time at start of tow (UTC); format: HH:MM
http://lod.bco-dmo.org/id/dataset-parameter/805677.rdf
Name: time_endtow
Units: unitless
Description: Time at end of tow (UTC); format: HH:MM
http://lod.bco-dmo.org/id/dataset-parameter/805678.rdf
Name: species
Units: unitless
Description: The planktic foraminifer species measured with the following abbreviations: bul = G. bulloides; glu = G. glutinata; hir = G. hirsuta; inc = N. incompta; inf = G. inflata; pac = N. pachyderma; qui = T. quinqueloba; tru = G. truncatulinoides; uni = O. universa
http://lod.bco-dmo.org/id/dataset-parameter/805679.rdf
Name: type
Units: unitless
Description: The kind of foraminiferal organic matter (i.e., tissue or shell-bound) measured for N isotope composition
http://lod.bco-dmo.org/id/dataset-parameter/805680.rdf
Name: analysis_year
Units: unitless
Description: The year in which foraminifer samples were measured for N isotope composition. The measurement protocol differed slightly between the three analysis sessions (see Methods Section and Supplemental Text S1 of Smart et al. (2020) for more detail).
http://lod.bco-dmo.org/id/dataset-parameter/805681.rdf
Name: latitude
Units: decimal degrees
Description: Latitude; positive values = North
http://lod.bco-dmo.org/id/dataset-parameter/805682.rdf
Name: longitude
Units: decimal degrees
Description: Longitude; positive values = East
http://lod.bco-dmo.org/id/dataset-parameter/805683.rdf
Name: depth_tow
Units: meters (m)
Description: Depth of the tow
http://lod.bco-dmo.org/id/dataset-parameter/805684.rdf
Name: d15N_foram_avg
Units: per mil vs AIR
Description: Average d15N. d15N is the nitrogen isotopic composition of a sample expressed in delta notation (d15N in units of per mil, ‰) relative to atmospheric N2, where d15N = {[(15N/14N)sample/(15N/14N)atmN2] – 1} × 1000
http://lod.bco-dmo.org/id/dataset-parameter/805685.rdf
Name: d15N_foram_sd
Units: per mil vs AIR
Description: Standard deviation of d15N_foram_avg
http://lod.bco-dmo.org/id/dataset-parameter/805686.rdf
Name: d15N_foram_n
Units: unitless
Description: Number of replicate measurements of d15N_foram_avg
http://lod.bco-dmo.org/id/dataset-parameter/805687.rdf
Name: indiv_avg
Units: unitless
Description: Average number of individual foraminifer specimens combined to make a measurement
http://lod.bco-dmo.org/id/dataset-parameter/805688.rdf
Name: indiv_sd
Units: unitless
Description: Standard deviation of indiv_avg
http://lod.bco-dmo.org/id/dataset-parameter/805689.rdf
Name: indiv_n
Units: unitless
Description: Number of replicate measurements of indiv_avg
http://lod.bco-dmo.org/id/dataset-parameter/805690.rdf
Name: Ni_avg
Units: nanomoles per individual (nmol/indiv)
Description: Average nitrogen contents of foraminifera per individual
http://lod.bco-dmo.org/id/dataset-parameter/805691.rdf
Name: Ni_sd
Units: nanomoles per individual (nmol/indiv)
Description: Standard deviation of Ni_avg
http://lod.bco-dmo.org/id/dataset-parameter/805692.rdf
Name: Ni_n
Units: unitless
Description: Number of replicate measurements of Ni_avg
http://lod.bco-dmo.org/id/dataset-parameter/805693.rdf
Name: Nw_avg
Units: nanomoles per milligram (nmol/mg)
Description: Average nitrogen contents of foraminifera per milligram
http://lod.bco-dmo.org/id/dataset-parameter/805694.rdf
Name: Nw_sd
Units: nanomoles per milligram (nmol/mg)
Description: Standard deviation of Nw_avg
http://lod.bco-dmo.org/id/dataset-parameter/805695.rdf
Name: Nw_n
Units: unitless
Description: Number of replicate measurements of Nw_avg
http://lod.bco-dmo.org/id/dataset-parameter/805696.rdf
Name: d15N_avg_nitrate
Units: per mil vs AIR
Description: Average d15N. d15N is the nitrogen isotopic composition of a sample (nitrate only) expressed in delta notation (d15N in units of per mil, ‰) relative to atmospheric N2, where d15N = {[(15N/14N)sample/(15N/14N)atmN2] – 1} × 1000
http://lod.bco-dmo.org/id/dataset-parameter/805697.rdf
Name: conc_avg_nitrate
Units: micromolar (uM)
Description: Concentration of nitrate in a seawater sample
http://lod.bco-dmo.org/id/dataset-parameter/805698.rdf
Name: d15N_avg_nitrate_nitrite
Units: per mil vs AIR
Description: Average d15N. d15N is the nitrogen isotopic composition of a sample (nitrate+nitrite) expressed in delta notation (d15N in units of per mil, ‰) relative to atmospheric N2, where d15N = {[(15N/14N)sample/(15N/14N)atmN2] – 1} × 1000
http://lod.bco-dmo.org/id/dataset-parameter/805699.rdf
Name: conc_avg_nitrate_nitrite
Units: micromolar (uM)
Description: Concentration of nitrate+nitrite in a seawater sample
http://lod.bco-dmo.org/id/dataset-parameter/805700.rdf
Name: temperature_ctd
Units: degrees Celsius
Description: Temperature measured by CTD
http://lod.bco-dmo.org/id/dataset-parameter/805701.rdf
Name: salinity_ctd
Units: psu
Description: Salinity measured by CTD
http://lod.bco-dmo.org/id/dataset-parameter/805702.rdf
Name: oxygen_ctd
Units: milliliters per liter (ml/l)
Description: Oxygen measured by CTD
http://lod.bco-dmo.org/id/dataset-parameter/805703.rdf
Name: fluorescence_ctd
Units: milligrams per cubic meter (mg/m3)
Description: Fluorescence measured by CTD
http://lod.bco-dmo.org/id/dataset-parameter/805704.rdf
Name: potdens_calc
Units: kilograms per cubic meter (kg/m3)
Description: Potential density, calculated from temperature and salinity
http://lod.bco-dmo.org/id/dataset-parameter/805705.rdf
Name: MLD_calc
Units: meters (m)
Description: Calculated mixed layer depth at each profile station, defined as the closest depth to the surface at which potential density is greater by greater than or equal to 0.03 kg/m3 than the value at a reference depth of 11 m (the shallowest depth common to every CTD station), based on the criterion of de Boyer Montégut et al. (2004).
http://lod.bco-dmo.org/id/dataset-parameter/805706.rdf
Name: conc_PON_avg
Units: micromolar (uM)
Description: Average concentration of PON. PON is the bulk (> 0.3 um) particulate organic nitrogen in surface waters filtered from the ship’s underway intake during the net tow.
http://lod.bco-dmo.org/id/dataset-parameter/805707.rdf
Name: conc_PON_sd
Units: micromolar (uM)
Description: Standard deviation of PON concentration
http://lod.bco-dmo.org/id/dataset-parameter/805708.rdf
Name: conc_PON_n
Units: unitless
Description: Number of replicate measurements of PON concentration
http://lod.bco-dmo.org/id/dataset-parameter/805709.rdf
Name: d15N_PON_avg
Units: per mil vs AIR
Description: Average d15N of PON. d15N is the nitrogen isotopic composition of a sample (nitrate only) expressed in delta notation (d15N in units of per mil, ‰) relative to atmospheric N2, where d15N = {[(15N/14N)sample/(15N/14N)atmN2] – 1} × 1000
http://lod.bco-dmo.org/id/dataset-parameter/805710.rdf
Name: d15N_PON_sd
Units: per mil vs AIR
Description: Standard deviation of d15N of PON
http://lod.bco-dmo.org/id/dataset-parameter/805711.rdf
Name: d15N_PON_n
Units: unitless
Description: Number of replicate measurements of d15N of PON
http://lod.bco-dmo.org/id/dataset-parameter/805712.rdf
Name: d15N_zoop_avg
Units: per mil vs AIR
Description: Average d15N of zooplankton. zoop refers to the bulk (i.e.,
http://lod.bco-dmo.org/id/dataset-parameter/805713.rdf
Name: d15N_zoop_se
Units: per mil vs AIR
Description: Standard error of the d15N of zooplankton
http://lod.bco-dmo.org/id/dataset-parameter/805714.rdf
Name: d15N_zoop_n
Units: unitless
Description: Number of replicate measurements of the d15N of zooplankton
http://lod.bco-dmo.org/id/dataset-parameter/805715.rdf
Name: ISO_DateTime_UTC_starttow
Units: yyyy-MM-dd'T'HH:mm:ss'Z'
Description: Date and time (UTC) of start of tow formatted to ISO 8601 standard; format: yyyy-mm-ddTHH:MM:SSZ
http://lod.bco-dmo.org/id/dataset-parameter/805720.rdf
Name: ISO_DateTime_UTC_endtow
Units: unitless
Description: Date and time (UTC) of end of tow formatted to ISO 8601 standard; format: yyyy-mm-ddTHH:MM:SSZ
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
100468
https://darchive.mblwhoilibrary.org/bitstream/1912/25520/1/dataset-805653_southern-ocean-foraminifer-n-isotopes__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.805653.1
download
onLine
dataset
<p>Planktic foraminifera were collected by towing a 200-μm-mesh plankton net for 90 min at a target depth within the upper mixed layer. Approximately 90% of the tow material was preserved in a 5-10% pH-buffered formalin solution and stored at 4°C until sorting for foraminifera (Ren et al., 2012). The remaining 10% was size fractionated and frozen at −20°C for element and isotope analysis of zooplankton. During each tow, bulk (&gt;0.3 µm) suspended PON and nitrate samples were collected from the underway intake (at 7 m depth) and frozen at −80°C and −20°C, respectively, until isotope analysis. <em>In situ</em> (i.e., tow-depth) nitrate samples and auxiliary hydrographic data come from CTD casts performed on station before the start of each net tow.</p>
<p>Foraminifera were separated by species, rinsed briefly with Milli-Q and crushed open to expose (non-shell-bound) tissue. Tissue N was converted to nitrate using the persulfate oxidation method (Nydahl, 1978; Knapp et al., 2005). Remnant shell material (having been oxidatively cleaned) was transferred, rinsed six times with Milli-Q and oven dried at 50°C. Shell samples were then dissolved with hydrochloric acid to release shell-bound organics for persulfate oxidation (Nydahl, 1978; Knapp et al., 2005). For both tissue- and shell-derived nitrate (from persulfate oxidation) as well as seawater nitrate, samples were measured for concentration by chemiluminescence (Braman &amp; Hendrix, 1989) and N isotope composition using the denitrifier method and gas chromatography−isotope ratio mass spectrometry (GC-IRMS) (Sigman et al., 2001; Casciotti et al., 2002; Weigand et al., 2016). The N isotope composition of bulk PON and size-fractionated zooplankton were measured by elemental analyser-IRMS.</p>
<p>The isotopic composition of N₂O was measured either at Princeton University (USA) or the Max Planck Institute for Chemistry (MPIC; Germany) by GC-IRMS using a Thermo MAT 253 mass spectrometer with a purpose-built on-line N₂O extraction and purification system. Bulk PON and size-fractionated zooplankton samples were measured at the University of Cape Town using a Thermo Scientific FLASH 2000 elemental analyzer coupled to a Thermo Scientific Delta V Plus mass spectrometer. Hydrographic data were acquired by a Sea-Bird conductivity-temperature depth sensor during each cast.</p>
<p>See methods section of Smart et al. (2020) for more detail.</p>
Specified by the Principal Investigator(s)
<p>At Princeton and MPIC, N isotope measurements were referenced against automated injections of N₂O from a gas cylinder, and then calibrated to N₂ in air using international reference materials: IAEA-N3 and USGS-34 nitrate standards in the case of seawater samples, or USGS-40 and USGS-41 amino acid standards in the case of foraminifer (oxidized) samples. Oxidized samples were additionally corrected for the contribution of the N blank associated with the persulfate oxidizing reagent.</p>
<p>At the University of Cape Town, N isotope measurements were referenced to atmospheric N₂ using three in-house organic standards: Choc, Merck Gel and Valine.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Sea-Bird conductivity-temperature depth sensor
Sea-Bird conductivity-temperature depth sensor
PI Supplied Instrument Name: Sea-Bird conductivity-temperature depth sensor Instrument Name: CTD Sea-Bird Instrument Short Name:CTD Sea-Bird Instrument Description: Conductivity, Temperature, Depth (CTD) sensor package from SeaBird Electronics, no specific unit identified. This instrument designation is used when specific make and model are not known. See also other SeaBird instruments listed under CTD. More information from Sea-Bird Electronics. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Thermo MAT 253 mass spectrometer
Thermo MAT 253 mass spectrometer
PI Supplied Instrument Name: Thermo MAT 253 mass spectrometer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Thermo Scientific Delta V Plus
Thermo Scientific Delta V Plus
PI Supplied Instrument Name: Thermo Scientific Delta V Plus Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
200-um-mesh plankton net
200-um-mesh plankton net
PI Supplied Instrument Name: 200-um-mesh plankton net Instrument Name: Plankton Net Instrument Short Name:Plankton Net Instrument Description: A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/22/
Thermo Scientific FLASH 2000
Thermo Scientific FLASH 2000
PI Supplied Instrument Name: Thermo Scientific FLASH 2000 Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Cruise: VOY016
VOY016
Community Standard Description
International Council for the Exploration of the Sea
R/V S.A. Agulhas II
vessel
Cruise: VOY019
VOY019
Community Standard Description
International Council for the Exploration of the Sea
R/V S.A. Agulhas II
vessel
Community Standard Description
International Council for the Exploration of the Sea
R/V S.A. Agulhas II
vessel