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Location: Cultures collected from Northeast Pacific Line P Transect 48.8167 N 128.667 W <\/p>\n
Metal quotas
\nCellular metal quotas were measured by inductively coupled plasma mass spectrometry (ICP-MS). Biomass from replicate 25 mL matrix cultures of P. tricornutum CCMP632, P. delicatissima UNC1205, and Thalassiosira UNC1203 were pooled upon entering stationary phase and were centrifuged at 11,000 RPM (14,610 x g) for 40 minutes at 4\u00b0C. The cell pellet was resuspended in ~1 mL media and transferred to an acid-cleaned microcentrifuge tube. Cultures were centrifuged again for 30 min at 14,100 RPM (13,336 x g) at 4\u00b0C before the supernatant was discarded. The remaining cell pellet was acidified in 800 L of 5% nitric acid (Optima) containing 1 ppb indium for at least seven days. Solids were removed by centrifugation. No attempt was made to remove extracellular metals by washing cells with additional metal chelators in order to minimize processing blanks. Quota determinations therefore include contributions from both intracellular and extracellular pools. Process blank digestions containing acid but no cells were performed in parallel. Digests were diluted by a factor of 9 with 5% nitric acid 1 ppb indium solution before being analyzed in duplicate on a Thermo ICAP-Q plasma mass spectrometer calibrated to a multi-element standard curve (Spex Certiprep) over a range of 1 \u2013 20 ppb. Samples were analyzed in KED mode after an 85s sample uptake window and element mass windows were scanned 3 times during measurements. The 1 ppb indium internal standard was used to correct for variation in sample delivery and plasma suppression between samples. Process blanks were subtracted from measured concentrations. Phosphorus concentrations were also measured by ICP-MS simultaneously and were calibrated to a standard curve ranging from 100 \u2013 3,200 ppb using a 1 ppm certified P stock (Alfa Aesar Specpure). The seawater media base used for all growth experiments was similarly analyzed via ICP-MS using a 1:10 dilution of media base into 5% nitric acid 1 ppb indium and analyzed as above to determine background media concentrations of total Zn and Co (0.9 nmol L-1 and 0.1 nmol L-1, respectively).<\/p>\n
Missing data identifiers in this dataset include:
\n* "nd" indicating no data
\n* Below Detection
\n* "contam" indicating the sample was contaminated<\/p>\n
Isolation sources and locations
\n* Pseudonitzschia delicatissima UNC1205 and Thalassiosira UNC1203 were isolated from
\nstation P8 of the Line P transect, 48.817\uf0b0N 128.666\uf0b0W
\n* Phaeodactylum tricornutum CCMP632 was ordered from Bigelow, the strains original
\nlocation of isolation was 54\uf0b0N 4\uf0b0W
\n* Thalassiosira pseudonana CCMP1335 was also from Bigelow, original location of
\nisolation was 40.756\u00b0 N 72.82\u00b0 W<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
Metal quotas of two NE Pacific Line P diatom isolates and one Atlantic diatom isolate measured via ICP-MS after growth in zinc and cobalt media amendments.\u00a0 Experiments with cultures collected from the GeoMICS expedition on the R\/V Thomas G. Thompson (cruise TN280), along Line P in the NE Pacific, in May of 2012.<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/www.w3.org\/2000\/01\/rdf-schema#label":[{"@value":"Metal quotas of Line P diatoms in added Zn and Co incubation studies","@type":"xsd:string"}],"http:\/\/ocean-data.org\/schema\/hasProcessingDescription":[{"@value":"
BCO-DMO Data Manager Processing Notes:
\n* Combined three tables in sheet \"Metal Quotas\"\u00a0 in originally submitted excel file \"BCO-DMO LineP Metal Quotas.xlsx\" and extracted to csv format.
\n* Column \"culture\" added to capture information in the title of each of the three original tables (P. delicatissima UNC1205,\u00a0Thalassiosira UNC1203,\u00a0P. tricornutum CCMP632)
\n* added a conventional header with dataset name, PI name, version date
\n* Missing data identifier in original data \"NA\" is displayed by default as \"nd\" in the bco-dmo data system.
\n* modified parameter names to conform with BCO-DMO naming conventions (only letters, numbers, and underscores)<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/purl.org\/dc\/terms\/identifier":[{"@value":"807299","@type":"xsd:int"}],"http:\/\/purl.org\/dc\/terms\/title":[{"@value":"Metal quotas of Line P diatoms in added Zn and Co incubation studies"}],"http:\/\/purl.org\/dc\/terms\/date":[{"@value":"2020-03-31T16:11:49-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/created":[{"@value":"2020-03-31T16:11:49-04:00","@type":"xsd:dateTime"}],"http:\/\/purl.org\/dc\/terms\/modified":[{"@value":"2021-08-31T11:30:31-04:00","@type":"xsd:dateTime"}],"http:\/\/rdfs.org\/ns\/void#inDataset":[{"@id":"http:\/\/www.bco-dmo.org\/"}],"http:\/\/ocean-data.org\/schema\/namedGraph":[{"@value":"urn:bcodmo:dataset:807299","@type":"xsd:token"}],"http:\/\/ocean-data.org\/schema\/osprey_page":[{"@id":"https:\/\/www.bco-dmo.org\/dataset\/807299"}],"http:\/\/ocean-data.org\/schema\/identifier":[{"@value":"_:Identifier807299"}],"http:\/\/ocean-data.org\/schema\/datasetTitle":[{"@value":"Metal quotas of NE Pacific and Altantic diatom isolates measured via ICP-MS after growth in zinc and cobalt media amendments in experiments with cultures collected from R\/V Thomas G. 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(2020) Metal quotas of NE Pacific and Altantic diatom isolates measured via ICP-MS after growth in zinc and cobalt media amendments in experiments with cultures collected from R\/V Thomas G. Thompson cruise TN280, along Line P in the NE Pacific, in May of 2012. Biological and Chemical Oceanography Data Management Office (BCO-DMO). 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