http://lod.bco-dmo.org/id/dataset/807402
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-04-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - Chlorophyll, particulate organic carbon and particulate organic nitrogen.
2020-04-01
publication
2020-04-01
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-31
publication
https://doi.org/10.26008/1912/bco-dmo.807402.1
Uta Passow
University of California-Santa Barbara
principalInvestigator
Edward Laws
Louisiana State University College of the Coast and Environment
principalInvestigator
Nigel D'Souza
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Passow, U., Laws, E., D'Souza, N. (2020) Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - Chlorophyll, particulate organic carbon and particulate organic nitrogen. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-04-01 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.807402.1 [access date]
Series 4A: POC, PON, chl-a Dataset Description: <p>The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria <em>Synechococcus elongatus</em> CCMP1629 in a multifactorial design. This dataset contains measurements of extracted chlorophyll, particulate organic carbon (POC), and particulate organic nitrogen (PON) made over the course of the experiments.</p> Methods and Sampling: <p><strong>Experimental setup:</strong></p>
<p>The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacterium S. elongatus CCMP1629 in a multifactorial design. Two CO2 concentrations were tested: 410 ppm, and 1000 ppm. For each CO2 concentration, four temperatures were tested: 20°C, 28°C, 36°C, and 44°C. Within each temperature, three light levels were tested: sub-optimum irradiance (SOI) intensity of 50 umol photons · m-2 · s-1, optimum irradiance (OI) intensity of 230 umol photons · m-2 · s-1 and extreme Irradiance (EI) intensity of 600 umol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at all four temperatures running simultaneously. This was repeated for each CO2 concentration.</p>
<p>Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from &lt;0.04 Liters per minute (LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days. For each CO2 and temperature level, replication was achieved by incubating three tubes at sub-optimum light intensities, two tubes at optimum light intensity, and three tubes at extreme light intensities. Each experiment was split into two phases: An acclimation phase spanning 3 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 3-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 5 hours into the daily light cycle to minimize effects of diurnal cycles.</p>
<p>Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), and phosphate (PO4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson &amp; Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol · kg−1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with the S. elongatus CCMP 1629 (SE1629) stock culture at the start of the experiments.</p>
<p><strong>Organic Carbon and Nitrogen concentrations</strong></p>
<p>Samples were filtered onto pre-combusted GF/F filters, dried at 60°C, and stored at room temperature until analyses of particulate organic carbon (POC), and particulate organic nitrogen (PON). Between 3 and &gt; 10 mL were filtered, with larger filtration volumes used on the final day of the experiment. Samples were analyzed using an elemental analyzer (CEC 44OHA; Control Equipment). Samples where C or N concentrations were below instrument detection limits were flagged.</p>
<p><strong>Chlorophyll</strong></p>
<p>Daily subsamples from each treatment were filtered onto 0.45 um polycarbonate filters and stored at -20°C. Filters were placed in 90% acetone (v/v) overnight at -20°C, and the extracted chlorophyll was measured fluorometrically on a Turner 700 fluorometer (Strickland 1972). Chlorophyll-a liquid standards in 90% acetone (Turner Designs Inc.), and adjustable solid secondary standards (Turner Designs Inc. P/N 8000-952) were used for calibrations, and to calculate the chlorophyll content of the samples.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538602 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538602
completed
Uta Passow
University of California-Santa Barbara
709-864-8010
Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay
St. John's
Newfoundland
A1C 5S7
Canada
uta.passow@mun.ca
pointOfContact
Edward Laws
Louisiana State University College of the Coast and Environment
225 578-3334
Louisiana State University School of the Coast and Environment 1002R Energy, Coast and Environment Building
Baton Rouge
LA
70803
USA
edlaws@lsu.edu
pointOfContact
Nigel D'Souza
University of California-Santa Barbara
419-819-9039
Marine Science Institute
nigel.dsouza@lifesci.ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Phase
CO2
Temp
Day
Irradiance
Tube
Vol_filtered_CHN
C_ug
N_ug
C_N
C_detect_lim
N_detect_lim
Vol_filtered_Chl
Chl_ug_L
Multicultivator MC-1000 OD (Qubit Systems)
Elemental analyzer (CEC 44OHA; Control Equipment)
Turner 700 fluorometer
theme
None, User defined
No BCO-DMO term
Partial pressure of CO2
water temperature at measurement depth
days
irradiance
sample identification
volume of water filtered
Elemental carbon (C)
Nitrogen
Carbon to Nitrogen ratio (C:N)
chlorophyll a
featureType
BCO-DMO Standard Parameters
Cell Cultivator
CHN Elemental Analyzer
Fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
https://osprey.bco-dmo.org/project/654347
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
<p>The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.</p>
<p>Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.</p>
Stressors on Marine Phytoplankton
largerWorkCitation
project
eng; USA
biota
oceans
2019-07-01
2019-08-31
0
BCO-DMO catalogue of parameters from Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - Chlorophyll, particulate organic carbon and particulate organic nitrogen.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/808192.rdf
Name: Phase
Units: unitless
Description: <p>Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808193.rdf
Name: CO2
Units: parts per million (ppm)
Description: <p>Indicates the concentration of CO2 in the CO2-Air mix that was bubbled through the samples over the course of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/808194.rdf
Name: Temp
Units: degrees Celsius
Description: <p>Indicates the temperature at which the samples were incubated.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808195.rdf
Name: Day
Units: day
Description: <p>Indicates the timepoint (day) of sampling. 0 = day 0; 1 = day 1; etc.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808196.rdf
Name: Irradiance
Units: micromol photons/meter^2/second
Description: <p>Indicates the irradiance at which the samples were incubated: SOI = sub-optimum irradiance intensity of 50 umol photons · m-2 · s-1; OI = optimum irradiance intensity of 230 umol photons · m-2 · s-1; and EI = extreme irradiance intensity of 600 umol photons · m-2 · s-1.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808197.rdf
Name: Tube
Units: unitless
Description: <p>Indicates the tube number in the multicultivator. The tube numbers indicate replication within a treatment: T1-T3 = suboptimum irradiance; T4-T5 = optimum irradiance; T6-T8 = extreme irradiance</p>
http://lod.bco-dmo.org/id/dataset-parameter/808198.rdf
Name: Vol_filtered_CHN
Units: milliliters
Description: <p>Indicates the volume of sample (in ml) filtered for CHN analyses</p>
http://lod.bco-dmo.org/id/dataset-parameter/808199.rdf
Name: C_ug
Units: micrograms
Description: <p>Organic carbon concentration in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/808200.rdf
Name: N_ug
Units: micrograms
Description: <p>Organic Nitrogen concentration in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/808201.rdf
Name: C_N
Units: unitless
Description: <p>Ratio of organic carbon to organic nitrogen in a sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/808202.rdf
Name: C_detect_lim
Units: micrograms
Description: <p>Indicates the organic carbon detection limit (DL) of the instrument at the time each sample was analyzed</p>
http://lod.bco-dmo.org/id/dataset-parameter/808203.rdf
Name: N_detect_lim
Units: micrograms
Description: <p>Indicates the organic carbon detection limit (DL) of the instrument at the time each sample was analyzed</p>
http://lod.bco-dmo.org/id/dataset-parameter/808204.rdf
Name: Vol_filtered_Chl
Units: milliliters
Description: <p>Volume of the sample that was filtered for chlorophyll analyses</p>
http://lod.bco-dmo.org/id/dataset-parameter/808205.rdf
Name: Chl_ug_L
Units: micrograms/liter
Description: <p>Chlorophyll concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
16545
https://darchive.mblwhoilibrary.org/bitstream/1912/26150/1/dataset-807402_series-4a-poc-pon-chl__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.807402.1
download
onLine
dataset
<p><strong>Experimental setup:</strong></p>
<p>The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacterium S. elongatus CCMP1629 in a multifactorial design. Two CO2 concentrations were tested: 410 ppm, and 1000 ppm. For each CO2 concentration, four temperatures were tested: 20°C, 28°C, 36°C, and 44°C. Within each temperature, three light levels were tested: sub-optimum irradiance (SOI) intensity of 50 umol photons · m-2 · s-1, optimum irradiance (OI) intensity of 230 umol photons · m-2 · s-1 and extreme Irradiance (EI) intensity of 600 umol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at all four temperatures running simultaneously. This was repeated for each CO2 concentration.</p>
<p>Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from &lt;0.04 Liters per minute (LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days. For each CO2 and temperature level, replication was achieved by incubating three tubes at sub-optimum light intensities, two tubes at optimum light intensity, and three tubes at extreme light intensities. Each experiment was split into two phases: An acclimation phase spanning 3 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 3-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 5 hours into the daily light cycle to minimize effects of diurnal cycles.</p>
<p>Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), and phosphate (PO4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson &amp; Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol · kg−1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with the S. elongatus CCMP 1629 (SE1629) stock culture at the start of the experiments.</p>
<p><strong>Organic Carbon and Nitrogen concentrations</strong></p>
<p>Samples were filtered onto pre-combusted GF/F filters, dried at 60°C, and stored at room temperature until analyses of particulate organic carbon (POC), and particulate organic nitrogen (PON). Between 3 and &gt; 10 mL were filtered, with larger filtration volumes used on the final day of the experiment. Samples were analyzed using an elemental analyzer (CEC 44OHA; Control Equipment). Samples where C or N concentrations were below instrument detection limits were flagged.</p>
<p><strong>Chlorophyll</strong></p>
<p>Daily subsamples from each treatment were filtered onto 0.45 um polycarbonate filters and stored at -20°C. Filters were placed in 90% acetone (v/v) overnight at -20°C, and the extracted chlorophyll was measured fluorometrically on a Turner 700 fluorometer (Strickland 1972). Chlorophyll-a liquid standards in 90% acetone (Turner Designs Inc.), and adjustable solid secondary standards (Turner Designs Inc. P/N 8000-952) were used for calibrations, and to calculate the chlorophyll content of the samples.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- changed "- NA -" to "nd", no data</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Multicultivator MC-1000 OD (Qubit Systems)
Multicultivator MC-1000 OD (Qubit Systems)
PI Supplied Instrument Name: Multicultivator MC-1000 OD (Qubit Systems) PI Supplied Instrument Description:Used for incubation of TP1014 cultures. Instrument Name: Cell Cultivator Instrument Short Name: Instrument Description: An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
Elemental analyzer (CEC 44OHA; Control Equipment)
Elemental analyzer (CEC 44OHA; Control Equipment)
PI Supplied Instrument Name: Elemental analyzer (CEC 44OHA; Control Equipment) PI Supplied Instrument Description:Used for analysis of total organic carbon content. Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
Turner 700 fluorometer
Turner 700 fluorometer
PI Supplied Instrument Name: Turner 700 fluorometer PI Supplied Instrument Description:Used for fluorometric analyses of extracted chlorophyll. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/