http://lod.bco-dmo.org/id/dataset/808215
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-04-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - computed photophysiology parameters
2020-04-03
publication
2020-04-03
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-31
publication
https://doi.org/10.26008/1912/bco-dmo.808215.1
Uta Passow
University of California-Santa Barbara
principalInvestigator
Edward Laws
Louisiana State University College of the Coast and Environment
principalInvestigator
Nigel D'Souza
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Passow, U., Laws, E., D'Souza, N. (2020) Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - computed photophysiology parameters. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-04-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.808215.1 [access date]
Series 4A: photophysiology COMPUTED Dataset Description: <p>The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria S. elongatus CCMP1629 in a multifactorial design. This dataset contains measurements of photophysiology using the Light curve (LC3) protocol of the Aquapen-C AP-C 100 fluorometer. They include the minimum fluorescence in dark-adapted state (Fo), the maximum fluorescence in dark-adapted state (Fm), the maximum Quantum yield (QY_max),&nbsp;measurements of the maximum fluorescence following exposure to actinic light (Fm_L#), the instantaneous fluorescence during light adaptation (Ft_L#), and measurements of the instantaneous photosystem II quantum yield following exposure to actinic light (QY_L#).</p>
<p>&nbsp;</p> Methods and Sampling: <p><strong>Experimental setup:</strong></p>
<p>The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacterium S. elongatus CCMP1629 in a multifactorial design. Two CO2 concentrations were tested: 410 ppm, and 1000 ppm. For each CO2 concentration, four temperatures were tested: 20°C, 28°C, 36°C, and 44°C. Within each temperature, three light levels were tested: sub-optimum irradiance (SOI) intensity of 50 umol photons · m-2 · s-1, optimum irradiance (OI) intensity of 230 umol photons · m-2 · s-1 and extreme Irradiance (EI) intensity of 600 umol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at all four temperatures running simultaneously. This was repeated for each CO2 concentration.</p>
<p>Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from &lt;0.04 Liters per minute (LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days. For each CO2 and temperature level, replication was achieved by incubating three tubes at sub-optimum light intensities, two tubes at optimum light intensity, and three tubes at extreme light intensities. Each experiment was split into two phases: An acclimation phase spanning 3 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 3-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 5 hours into the daily light cycle to minimize effects of diurnal cycles.</p>
<p>Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), and phosphate (PO4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration, and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson &amp; Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol · kg−1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with the S. elongatus CCMP 1629 (SE1629) stock culture at the start of the experiments.</p>
<p><strong>Photophysiology</strong></p>
<p>Photophysiology was assessed daily using a handheld Pulse Amplitude Modulated (PAM) fluorometer (AquaPen-C AP-C 100, Photon System Instruments, Czech Republic). A sample was collected from each tube, 5 hours after the start of the daily light cycle, and placed in the dark for a minimum of 30 minutes prior to measurements. The dark-adapted sample was used to generate light curves that provide measurements of in-vivo chlorophyll autofluorescence (F0), the maximum quantum yield (QYmax or Fv/Fm), and relative photosynthesis rates based on PSII quantum yields at varying light intensities - using the instrument’s LC3 protocol. The LC3 protocol involves measurements of baseline and maximal fluorescence over seven 60-second phases, with each phase representing a light intensity from 10 to 1000 umol photons m-2 · s-1.&nbsp; Red light (620 nm) was used as actinic light in these experiments, and measurements were made at measuring illumination (f-pulse) intensity of 0.03 umol photons m-2 · s-1, and saturating (F-pulse) illumination of 2700 umol photons m-2 · s-1, and actinic illumination (A-pulse) controlled by the instrument’s protocol were set at 10, 20, 50, 100, 300, 500,and 1000&nbsp; umol photons m-2 · s-1 (for each 60-second phase).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538602 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538602
completed
Uta Passow
University of California-Santa Barbara
709-864-8010
Ocean Sciences Centre, Memorial University Marine Lab Road, Logy Bay
St. John's
Newfoundland
A1C 5S7
Canada
uta.passow@mun.ca
pointOfContact
Edward Laws
Louisiana State University College of the Coast and Environment
225 578-3334
Louisiana State University School of the Coast and Environment 1002R Energy, Coast and Environment Building
Baton Rouge
LA
70803
USA
edlaws@lsu.edu
pointOfContact
Nigel D'Souza
University of California-Santa Barbara
419-819-9039
Marine Science Institute
nigel.dsouza@lifesci.ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Phase
CO2
Temperature
Day
Irradiance
Fo
Fm
QY_max
Fm_L1
Fm_L2
Fm_L3
Fm_L4
Fm_L5
Fm_L6
Fm_L7
Ft_L1
Ft_L2
Ft_L3
Ft_L4
Ft_L5
Ft_L6
Ft_L7
QY_L1
QY_L2
QY_L3
QY_L4
QY_L5
QY_L6
QY_L7
Tube
Multicultivator MC-1000 OD (Qubit Systems)
Aquapen-C AP-C 100 (Photon Systems Instruments)
Genesys 10SVIS
theme
None, User defined
sample description
Partial pressure of CO2
water temperature at measurement depth
days
treatment
fluorescence
Fv to Fm ratio
replicate
featureType
BCO-DMO Standard Parameters
Cell Cultivator
Fluorometer
Spectrophotometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
https://osprey.bco-dmo.org/project/654347
Collaborative Research: Effects of multiple stressors on Marine Phytoplankton
<p>The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.</p>
<p>Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.</p>
Stressors on Marine Phytoplankton
largerWorkCitation
project
eng; USA
biota
oceans
2019-07-01
2019-08-31
0
BCO-DMO catalogue of parameters from Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - computed photophysiology parameters
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/808229.rdf
Name: Phase
Units: unitless
Description: <p>Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808230.rdf
Name: CO2
Units: parts per million (ppm)
Description: <p>Indicates the concentration of CO2 in the CO2-Air mix that was bubbled through the samples over the course of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/808231.rdf
Name: Temperature
Units: degrees Celsius
Description: <p>Indicates the temperature at which the samples were incubated.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808232.rdf
Name: Day
Units: unitless
Description: <p>Indicates the timepoint (day) of sampling. D0 = day 0; D1 = day 1; etc.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808234.rdf
Name: Irradiance
Units: unitless
Description: <p>Irradiance level: SOL = sub-optimum light; OL = optimum light; EL = extreme light</p>
http://lod.bco-dmo.org/id/dataset-parameter/808235.rdf
Name: Fo
Units: RFU (Relative Fluorscence Units)
Description: <p>minimum fluorescence in dark-adapted state.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808236.rdf
Name: Fm
Units: RFU (Relative Fluorscence Units)
Description: <p>the maximum fluorescence in dark-adapted state; measured during the first saturation flash after dark adaptation</p>
http://lod.bco-dmo.org/id/dataset-parameter/808237.rdf
Name: QY_max
Units: unitless
Description: <p>The maximum Quantum yield. A measure of the Photosystem II efficiency. In a dark-adapted sample this is equivalent to Fv/Fm. In a light-adapted sample it is equivalent to Fv’/Fm’.</p>
http://lod.bco-dmo.org/id/dataset-parameter/808238.rdf
Name: Fm_L1
Units: RFU (Relative Fluorscence Units)
Description: <p>The first measurement of the maximum fluorescence following exposure to actinic light at 10 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808239.rdf
Name: Fm_L2
Units: RFU (Relative Fluorscence Units)
Description: <p>The second measurement of the maximum fluorescence following exposure to actinic light at 20 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808240.rdf
Name: Fm_L3
Units: RFU (Relative Fluorscence Units)
Description: <p>The third measurement of the maximum fluorescence following exposure to actinic light at 50 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808241.rdf
Name: Fm_L4
Units: RFU (Relative Fluorscence Units)
Description: <p>The fourth measurement of the maximum fluorescence following exposure to actinic light at 100 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808242.rdf
Name: Fm_L5
Units: RFU (Relative Fluorscence Units)
Description: <p>The fifth measurement of the maximum fluorescence following exposure to actinic light at 300 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808243.rdf
Name: Fm_L6
Units: RFU (Relative Fluorscence Units)
Description: <p>The sixth measurement of the maximum fluorescence following exposure to actinic light at 500 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808244.rdf
Name: Fm_L7
Units: RFU (Relative Fluorscence Units)
Description: <p>The seventh measurement of the maximum fluorescence following exposure to actinic light at 1000 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808245.rdf
Name: Ft_L1
Units: RFU (Relative Fluorscence Units)
Description: <p>The first measurement of the instantaneous fluorescence during light adaptation following exposure to actinic light at 10 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808246.rdf
Name: Ft_L2
Units: RFU (Relative Fluorscence Units)
Description: <p>The second measurement of the instantaneous fluorescence during light adaptation following exposure to actinic light at 20 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808247.rdf
Name: Ft_L3
Units: RFU (Relative Fluorscence Units)
Description: <p>The third measurement of the instantaneous fluorescence during light adaptation following exposure to actinic light at 50 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808248.rdf
Name: Ft_L4
Units: RFU (Relative Fluorscence Units)
Description: <p>The fourth measurement of the instantaneous fluorescence during light adaptation following exposure to actinic light at 100 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808249.rdf
Name: Ft_L5
Units: RFU (Relative Fluorscence Units)
Description: <p>The fifth measurement of the instantaneous fluorescence during light adaptation following exposure to actinic light at 300 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808250.rdf
Name: Ft_L6
Units: RFU (Relative Fluorscence Units)
Description: <p>The sixth measurement of the instantaneous fluorescence during light adaptation following exposure to actinic light at 500 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808251.rdf
Name: Ft_L7
Units: RFU (Relative Fluorscence Units)
Description: <p>The seventh measurement of the instantaneous fluorescence during light adaptation following exposure to actinic light at 1000 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808252.rdf
Name: QY_L1
Units: unitless
Description: <p>The first measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 10 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808253.rdf
Name: QY_L2
Units: unitless
Description: <p>The second measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 20 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808254.rdf
Name: QY_L3
Units: unitless
Description: <p>The third measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 50 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808255.rdf
Name: QY_L4
Units: unitless
Description: <p>The fourth measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 100 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808256.rdf
Name: QY_L5
Units: unitless
Description: <p>The fifth measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 300 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808257.rdf
Name: QY_L6
Units: unitless
Description: <p>The sixth measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 500 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/808258.rdf
Name: QY_L7
Units: unitless
Description: <p>The seventh measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 1000 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the "light" phase)</p>
http://lod.bco-dmo.org/id/dataset-parameter/822897.rdf
Name: Tube
Units: unitless
Description: <p>Indicates the tube number in the multicultivator. The tube numbers Indicate replication within a treatment: T1-T3 = suboptimum irradiance; T4-T5 = optimum irradiance; and T6-T8 = extreme irradiance.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
38468
https://darchive.mblwhoilibrary.org/bitstream/1912/26153/1/dataset-808215_series-4a-photophysiology-computed__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.808215.1
download
onLine
dataset
<p><strong>Experimental setup:</strong></p>
<p>The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacterium S. elongatus CCMP1629 in a multifactorial design. Two CO2 concentrations were tested: 410 ppm, and 1000 ppm. For each CO2 concentration, four temperatures were tested: 20°C, 28°C, 36°C, and 44°C. Within each temperature, three light levels were tested: sub-optimum irradiance (SOI) intensity of 50 umol photons · m-2 · s-1, optimum irradiance (OI) intensity of 230 umol photons · m-2 · s-1 and extreme Irradiance (EI) intensity of 600 umol photons · m-2 · s-1. All lights were set at a 12 h day: 12 h dark cycle. For logistical reasons, experiments were partially conducted in series, with all light treatments at all four temperatures running simultaneously. This was repeated for each CO2 concentration.</p>
<p>Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-tubes immersed in a thermostated water bath, each independently illuminated by an array of cool white LEDs set at specific intensity and timing. A 0.2um filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile artificial seawater, and the humidified gas mix was supplied to each tube via gentle sparging through a 2um stainless steel diffuser. Flow rates were gradually increased over the course of the incubation to compensate for the DIC uptake of actively growing cells, and ranged from &lt;0.04 Liters per minute (LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days. For each CO2 and temperature level, replication was achieved by incubating three tubes at sub-optimum light intensities, two tubes at optimum light intensity, and three tubes at extreme light intensities. Each experiment was split into two phases: An acclimation phase spanning 3 days, was used to acclimate cultures to their new environment. Pre-acclimated, exponentially-growing cultures were then inoculated into fresh media and incubated through a 3-day experimental phase during which assessments of growth, photophysiology, and nutrient cycling were carried out daily. All sampling started 5 hours into the daily light cycle to minimize effects of diurnal cycles.</p>
<p>Experiments were conducted with artificial seawater (ASW) prepared using previously described methods (Kester et. al 1967), and enriched with nitrate (NO3), and phosphate (PO4), at levels ensuring that the cultures would remain nutrient-replete over the course of the experiment. Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected DIC concentration, and pH of the growth media was determined for the different pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with constants from Mehrbach et al. (1973, refit by Dickson &amp; Millero 1987), and inputs of temperature, salinity, total alkalinity (2376.5 umol · kg−1), pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each phase of the experiments were manipulated by the addition of NaHCO3, and was then maintained by bubbling a CO2-Air mix through the cultures over the course of the experiments. The pH of the growth media was measured spectrophometrically using the m-cresol purple method (Dickson 1993), and adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml aliquots and each aliquot was inoculated with the S. elongatus CCMP 1629 (SE1629) stock culture at the start of the experiments.</p>
<p><strong>Photophysiology</strong></p>
<p>Photophysiology was assessed daily using a handheld Pulse Amplitude Modulated (PAM) fluorometer (AquaPen-C AP-C 100, Photon System Instruments, Czech Republic). A sample was collected from each tube, 5 hours after the start of the daily light cycle, and placed in the dark for a minimum of 30 minutes prior to measurements. The dark-adapted sample was used to generate light curves that provide measurements of in-vivo chlorophyll autofluorescence (F0), the maximum quantum yield (QYmax or Fv/Fm), and relative photosynthesis rates based on PSII quantum yields at varying light intensities - using the instrument’s LC3 protocol. The LC3 protocol involves measurements of baseline and maximal fluorescence over seven 60-second phases, with each phase representing a light intensity from 10 to 1000 umol photons m-2 · s-1.&nbsp; Red light (620 nm) was used as actinic light in these experiments, and measurements were made at measuring illumination (f-pulse) intensity of 0.03 umol photons m-2 · s-1, and saturating (F-pulse) illumination of 2700 umol photons m-2 · s-1, and actinic illumination (A-pulse) controlled by the instrument’s protocol were set at 10, 20, 50, 100, 300, 500,and 1000&nbsp; umol photons m-2 · s-1 (for each 60-second phase).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- changed "NA" to "nd", no data<br />
- unpivoted the top 6 header rows to create a flat table<br />
- concatenated the 410 and 1000 pCO2 tables</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Multicultivator MC-1000 OD (Qubit Systems)
Multicultivator MC-1000 OD (Qubit Systems)
PI Supplied Instrument Name: Multicultivator MC-1000 OD (Qubit Systems) PI Supplied Instrument Description:Used for incubation of TP1014 cultures. Instrument Name: Cell Cultivator Instrument Short Name: Instrument Description: An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.
Aquapen-C AP-C 100 (Photon Systems Instruments)
Aquapen-C AP-C 100 (Photon Systems Instruments)
PI Supplied Instrument Name: Aquapen-C AP-C 100 (Photon Systems Instruments) PI Supplied Instrument Description:For assessment of photochemistry. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Genesys 10SVIS
Genesys 10SVIS
PI Supplied Instrument Name: Genesys 10SVIS PI Supplied Instrument Description:For measurement of pH. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/