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(1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites;
(2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems;
(3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and
(4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists.
Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI.
Data Management:
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Despite being the largest ecosystem on earth, the deep biosphere is considered to be energy limited. Chemoautotrophy is an important source of organic carbon in the deep biosphere, and significantly contributes to the deep carbon cycle. We investigated the carbon fixation strategies in the model organism Themovibrio ammonificans in relationship to the presence of different terminal electron acceptor. T. ammonicans uses the reverse Tricarboxylic Acid cycle (rTCA) as carbon fixation pathways, however our comparative genomic analysis reveals the presence of an incomplete Wood-Ljungdahl (WL) pathway. Carbon isotopic fractionation value support the rTCA cycle as carbon fixation pathway, however a difference in carbon fractionation is present when growing T. ammonificans with sulfur instead of nitrate as terminal electron acceptor. Transcriptomic analysis showed that the putative carbon monoxide dehydrogenase (type V) is expressed under both conditions. We also identified a putative oxidoreductase involved in the respiration of elemental sulfur, and propose a new pathway of sulfur reduction. The presence of incomplete yet functional alternative pathways of carbon fixation in subsurface organisms may be more widespread that previously thought, and may provide an evolutionary advantage in surviving under energy limiting conditions.&lt;/p&gt;
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                <gco:CharacterString>&amp;lt;p&amp;gt;Manual curation of the genome was performed using blastn and blastp against the non-redundant database, the conserved domain database, the Kyoto Encyclopedia of Genes and Genomes, and the PFAM database. Coding sequence similarities were compared using translated protein sequence. Metabolic pathways were reconstructed on the basis of available genomic, physiologic, and biochemical information and using Kyoto Encyclopedia of Genes and Genomes and SEED pathways as template.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Plots generated using this dataset can be found at:&amp;lt;br /&amp;gt;
&amp;lt;a href=&amp;quot;http://genomevolution.org/r/9vb3&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;http://genomevolution.org/r/9vb3&amp;lt;/a&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;a href=&amp;quot;http://genomevolution.org/r/9vb4&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;http://genomevolution.org/r/9vb4&amp;lt;/a&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;a href=&amp;quot;http://genomevolution.org/r/9vb8&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;http://genomevolution.org/r/9vb8&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;</gco:CharacterString>
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    <gmi:instrument gco:nilReason="unknown"/></gmi:MI_AcquisitionInformation>
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