http://lod.bco-dmo.org/id/dataset/809388
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-04-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius
2021-11-16
publication
2021-11-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-11-16
publication
https://doi.org/10.26008/1912/bco-dmo.809388.1
Casey terHorst
California State University Northridge
principalInvestigator
Mary Alice Coffroth
State University of New York at Buffalo
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Coffroth, M. A., terHorst, C. (2021) Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.809388.1 [access date]
Cell counts in coral polyps Dataset Description: <p>Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees C.</p> Methods and Sampling: <p>Larvae were collected from <em>Antillogorgia bipinnata </em>colonies that were maintained in seawater tables at the Keys Marine Lab on Long Key, FL.&nbsp; <em>Antillogorgia bipinnata </em>colonies were initially collected from Tennessee Reef (N 24° 45.150'&nbsp; W 81° 45.275')</p>
<p><em>Breviolum antillogorgium </em>cultures, representing five genotypes, were used to infect polyps reared from larvae collected from <em>A. bipinnata</em>. Three cultures had been grown at 26-degree C since isolation in 2016 (G1 [16-0590F], G2 [16-0875] and G3 [16-1631]) and two had been grown at 30-degree C since isolation in 2016 (G4 [16-0587] and G5 [16-0763]).&nbsp; Polyps from all treatments were reared at both 26 and 30 degree C in the lab under 12:12 light dark cycles. When the experiment was terminated (69 days after initial inoculations), polyps were sampled to determine cell density.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559286 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559286
completed
Casey terHorst
California State University Northridge
818-677-3352
Department of Biology 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
casey.terhorst@csun.edu
pointOfContact
Mary Alice Coffroth
State University of New York at Buffalo
716-645-4871
Department of Geology 126 Cooke Hall
Buffalo
NY
14260
USA
Coffroth@buffalo.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Container
Polyp_ID
Culture
Polyp_age
Temperature
Count_1
Count_2
Mean
Cells_ml
Cells_polyp
Reichert Brightline hemocytometer
Zeiss
theme
None, User defined
sample description
sample identification
age
water temperature at measurement depth
count
cell_concentration
featureType
BCO-DMO Standard Parameters
Hemocytometer
Microscope - Optical
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
https://osprey.bco-dmo.org/project/632538
RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs
<p><em>Description from NSF award abstract:</em><br />
On coral reefs, mutualisms with single celled algae (Symbiodinium) and reef species literally and figuratively form the foundation of reef ecosystems. Coral reefs are among the most threatened ecosystems under a changing climate and are rapidly declining due to increasing levels of environmental stress, namely increased temperatures. Climate change is resulting in even warmer ocean temperatures that threaten associations between Symbiodinium and their hosts. In this project the investigators examine the genetic diversity of Symbiodinium and the potential for this important species to evolve in response to temperature. The project will also address whether the ecological and evolutionary dynamics of the Symbiodinium population affect the performance of their host. If so, this suggests that the evolution of microscopic organisms with short generation times could confer adaptation to longer-lived host species on ecologically and economically vital coral reefs. Given that diversity is already being lost on many reefs, considering how evolutionary changes in Symbiodinium will affect reef species is crucial for predicting the responses of reefs to future climate change. This project provides training for two graduate students and several undergraduates at a Hispanic-serving institution. This work includes outreach to the students and the general public through the Aquarium of Niagara, local K-12 schools, and web-based education modules.</p>
<p>The effects of evolution on contemporary ecological processes are at the forefront of research in evolutionary ecology. This project will answer the call for experiments elucidating the effects of genetic variation in Symbiodinium performance and the effect on the response of the holobiont (host and symbiont) to increased temperature. These experiments examine the effects of temperature through both ecological and evolutionary mechanisms and will determine the relative importance of adaptation and acclimatization in replicated experimental populations. The investigators will examine how genetic variation within a species (Symbiodinium antillogorgium) affects symbiont performance in culture and in the host and how this affects the response of the holobiont to increased temperature. Further, the project examines whether holobiont response to increased temperature associated with climate change depends on particular GxG host-symbiont combinations. Moreover, the investigators will examine the effects of symbiont history on mutualist hosts, which have been largely ignored in eco-evolutionary studies. These experiments provide a first step in predicting whether invertebrate hosts on coral reefs will respond to global change via adaptation of their symbionts.</p>
Host Symbiont Temp Response
largerWorkCitation
project
eng; USA
biota
oceans
-81.754583
-81.754583
24.7525
24.7525
2018-01-01
2018-12-31
Florida Keys, Caribbean
0
BCO-DMO catalogue of parameters from Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees Celsius
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/855208.rdf
Name: Container
Units: unitless
Description: <p>Identification of container replicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/855209.rdf
Name: Polyp_ID
Units: unitless
Description: <p>Identification of polyp that was sampled given as container and mapped polyp number (eg. 2.06 would be container 2; mapped polyp 6)</p>
http://lod.bco-dmo.org/id/dataset-parameter/855210.rdf
Name: Culture
Units: unitless
Description: <p>Culture used to inoculate the polyps</p>
http://lod.bco-dmo.org/id/dataset-parameter/855211.rdf
Name: Polyp_age
Units: days
Description: <p>Age of the polyp</p>
http://lod.bco-dmo.org/id/dataset-parameter/855212.rdf
Name: Temperature
Units: Degrees C
Description: <p>Temperature at which the polyp was reared</p>
http://lod.bco-dmo.org/id/dataset-parameter/855213.rdf
Name: Count_1
Units: unitless
Description: <p>First replicate of cells counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/855214.rdf
Name: Count_2
Units: unitless
Description: <p>Second replicate of cells counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/855215.rdf
Name: Mean
Units: unitless
Description: <p>Mean of replicate counts</p>
http://lod.bco-dmo.org/id/dataset-parameter/855216.rdf
Name: Cells_ml
Units: Cells ml-1
Description: <p>Cells in one ml</p>
http://lod.bco-dmo.org/id/dataset-parameter/855217.rdf
Name: Cells_polyp
Units: Cells_polyp
Description: <p>Cells in the sampled polyp</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10606
https://darchive.mblwhoilibrary.org/bitstream/1912/27766/1/dataset-809388_cell-counts-polyps-___-fall-2018-experiment__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.809388.1
download
onLine
dataset
<p>Larvae were collected from <em>Antillogorgia bipinnata </em>colonies that were maintained in seawater tables at the Keys Marine Lab on Long Key, FL.&nbsp; <em>Antillogorgia bipinnata </em>colonies were initially collected from Tennessee Reef (N 24° 45.150'&nbsp; W 81° 45.275')</p>
<p><em>Breviolum antillogorgium </em>cultures, representing five genotypes, were used to infect polyps reared from larvae collected from <em>A. bipinnata</em>. Three cultures had been grown at 26-degree C since isolation in 2016 (G1 [16-0590F], G2 [16-0875] and G3 [16-1631]) and two had been grown at 30-degree C since isolation in 2016 (G4 [16-0587] and G5 [16-0763]).&nbsp; Polyps from all treatments were reared at both 26 and 30 degree C in the lab under 12:12 light dark cycles. When the experiment was terminated (69 days after initial inoculations), polyps were sampled to determine cell density.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Polyps (aged 92-115 days) were removed from incubation containers and homogenized in 0.1 ml filtered seawater. Two replicate cell counts of each polyp was made using light microscopy and a hemocytometer. Mean symbiont density per ml was calculated.</p>
<p>Mean x 10,000 were calculated from the counts to give cells/ml and then divided by 10 to give cells/polyp</p>
<p>&nbsp;</p>
<p><strong>BCO-DMO Processing notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Reichert Brightline hemocytometer
Reichert Brightline hemocytometer
PI Supplied Instrument Name: Reichert Brightline hemocytometer PI Supplied Instrument Description:Used to make cell counts. Instrument Name: Hemocytometer Instrument Short Name:Hemocytometer Instrument Description: A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
Zeiss
Zeiss
PI Supplied Instrument Name: Zeiss Instrument Name: Microscope - Optical Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope". Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/