http://lod.bco-dmo.org/id/dataset/810902
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-05-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Supplementary Table 1: Results of CHNS analyses in rock samples at different depths and sample images.
2020-05-11
publication
2020-05-11
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-07-08
publication
https://doi.org/10.26008/1912/bco-dmo.810902.1
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Edgcomb, V. (2020) Supplementary Table 1: Results of CHNS analyses in rock samples at different depths and sample images. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-05-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.810902.1 [access date]
Dataset Description: <p>Results of CHNS analyses in rock samples at different depths and sample images taken on board of the R/V JOIDES Resolution between November 30, 2015 and January 30, 2016.</p> Methods and Sampling: <p>Powderized rock material 462 from each sample (produced in the laminar flow hood on the <em>JOIDES</em> Resolution using a sterile mortar and pestle) was immediately transferred to sterile, muffled glass containers and stored in a desiccator until analysis of carbon and nitrogen according to established methods. Briefly, samples were weighed into methanol rinsed silver boats (4 x 6 mm, Costech). 96-well glass plates (combusted 4 hrs at 450°C) holding these samples were placed in a vacuum desiccator that also contained an open dish with about 50 mL fresh, concentrated (12N) HCl. An inverted crystallization dish was placed over the samplesto protect them from water condensation. The desiccator was closed and pumped out with an airdriven aspirator, to a reading of about ~ 0.5 atm and the desiccator was placed in an oven kept between 60 and 65 °C. Acidification was allowed to run for 60 to 72 hours. When acidification was complete, the samples were removed and set in the oven to dry (60-65 °C), and then placed in a vacuum desiccator charged with indicating silica gel (Fisher S162-500, activated by heating above 220 °C for several hours) and pumped down again and dried for about 24 hours.<br />
<br />
&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1658031 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1658031
completed
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
508-289-3734
Department of Geology and Geophysics 220 McLean Lab, MS #8
Woods Hole
MA
02543
USA
vedgcomb@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
Depth
TOC
TON
Total_C_Total_N
Ratio_13C_12C
Clay_OH
Observations
Reference_Picture
Latitude
Longitude
DeltaPlus isotope ratio mass spectrometer
Costech 4010 Elemental Analyzer
theme
None, User defined
sample identification
depth
total organic Carbon
total organic Nitrogen
Carbon to Nitrogen ratio
13C to 12C ratio in DIC
percent composition
sample description
latitude
longitude
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
Costech International Elemental Combustion System (ECS) 4010
instrument
BCO-DMO Standard Instruments
IODP-360
service
Deployment Activity
SW Indian Ridge, Indian Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
International Ocean Discovery Program
http://www.iodp.org/index.php
International Ocean Discovery Program
The International Ocean Discovery Program (IODP) is an international marine research collaboration that explores Earth's history and dynamics using ocean-going research platforms to recover data recorded in seafloor sediments and rocks and to monitor subseafloor environments. IODP depends on facilities funded by three platform providers with financial contributions from five additional partner agencies. Together, these entities represent 26 nations whose scientists are selected to staff IODP research expeditions conducted throughout the world's oceans.
IODP expeditions are developed from hypothesis-driven science proposals aligned with the program's science plan Illuminating Earth's Past, Present, and Future. The science plan identifies 14 challenge questions in the four areas of climate change, deep life, planetary dynamics, and geohazards.
IODP's three platform providers include:
The U.S. National Science Foundation (NSF)
Japan's Ministry of Education, Culture, Sports, Science and Technology (MEXT)
The European Consortium for Ocean Research Drilling (ECORD)
More information on IODP, including the Science Plan and Policies/Procedures, can be found on their website at http://www.iodp.org/program-documents.
IODP
largerWorkCitation
program
Collaborative Research: Delineating The Microbial Diversity and Cross-domain Interactions in The Uncharted Subseafloor Lower Crust Using Meta-omics and Culturing Approaches
https://www.bco-dmo.org/project/709556
Collaborative Research: Delineating The Microbial Diversity and Cross-domain Interactions in The Uncharted Subseafloor Lower Crust Using Meta-omics and Culturing Approaches
<p><strong>NSF abstract:</strong></p>
<p>The lower ocean crust has remained largely unexplored and represents one of the last frontiers for biological exploration on Earth. Preliminary data indicate an active subsurface biosphere in samples of the lower oceanic crust collected from Atlantis Bank in the SW Indian Ocean as deep as 790 m below the seafloor. Even if life exists in only a fraction of the habitable volume where temperatures permit and fluid flow can deliver carbon and energy sources, an active lower oceanic crust biosphere would have implications for deep carbon budgets and yield insights into microbiota that may have existed on early Earth. This is all of great interest to other research disciplines, educators, and students alike. A K-12 education program will capitalize on groundwork laid by outreach collaborator, A. Martinez, a 7th grade teacher in Eagle Pass, TX, who sailed as outreach expert on Drilling Expedition 360. Martinez works at a Title 1 school with ~98% Hispanic and ~2% Native American students and a high number of English Language Learners and migrants. Annual school visits occur during which the project investigators present hands on-activities introducing students to microbiology, and talks on marine microbiology, the project, and how to pursue science related careers. In addition, monthly Skype meetings with students and PIs update them on project progress. Students travel to the University of Texas Marine Science Institute annually, where they get a campus tour and a 3-hour cruise on the R/V Katy, during which they learn about and help with different oceanographic sampling approaches. The project partially supports two graduate students, a Woods Hole undergraduate summer student, the participation of multiple Texas A+M undergraduate students, and 3 principal investigators at two institutions, including one early career researcher who has not previously received NSF support of his own.</p>
<p>Given the dearth of knowledge of the lower oceanic crust, this project is poised to transform our understanding of life in this vast environment. The project assesses metabolic functions within all three domains of life in this crustal biosphere, with a focus on nutrient cycling and evaluation of connections to other deep marine microbial habitats. The lower ocean crust represents a potentially vast biosphere whose microbial constituents and the biogeochemical cycles they mediate are likely linked to deep ocean processes through faulting and subsurface fluid flow. Atlantis Bank represents a tectonic window that exposes lower oceanic crust directly at the seafloor. This enables seafloor drilling and research on an environment that can transform our understanding of connections between the deep subseafloor biosphere and the rest of the ocean. Preliminary analysis of recovered rocks from Expedition 360 suggests the interaction of seawater with the lower oceanic crust creates varied geochemical conditions capable of supporting diverse microbial life by providing nutrients and chemical energy. This project is the first interdisciplinary investigation of the microbiology of all 3 domains of life in basement samples that combines diversity and "meta-omics" analyses, analysis of nutrient addition experiments, high-throughput culturing and physiological analyses of isolates, including evaluation of their ability to utilize specific carbon sources, Raman spectroscopy, and lipid biomarker analyses. Comparative genomics are used to compare genes and pathways relevant to carbon cycling in these samples to data from published studies of other deep-sea environments. The collected samples present a rare and time-sensitive opportunity to gain detailed insights into microbial life, available carbon and energy sources for this life, and of dispersal of microbiota and connections in biogeochemical processes between the lower oceanic crust and the overlying aphotic water column.</p>
<p><strong>About the study area:</strong></p>
<p>The International Ocean Discovery Program (<a href="https://www.iodp.org/" target="_blank">IODP</a>) Expedition 360 explored the lower crust at Atlantis Bank, a 12 Ma oceanic core complex on the ultraslow-spreading SW Indian Ridge. This oceanic core complex represents a tectonic window that exposes lower oceanic crust and mantle directly at the seafloor, and the expedition provided an unprecedented opportunity to access this habitat in the Indian Ocean.</p>
Subseafloor Lower Crust Microbiology
largerWorkCitation
project
eng; USA
oceans
SW Indian Ridge, Indian Ocean
57.278183
57.278183
-32.70567
-32.70567
2015-11-30
2016-01-30
SW Indian Ridge, Indian Ocean
0
BCO-DMO catalogue of parameters from Supplementary Table 1: Results of CHNS analyses in rock samples at different depths and sample images.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/810933.rdf
Name: Sample_ID
Units: unitless
Description: Sample ID
http://lod.bco-dmo.org/id/dataset-parameter/810934.rdf
Name: Depth
Units: meters (m)
Description: Depth below sea floor
http://lod.bco-dmo.org/id/dataset-parameter/810935.rdf
Name: TOC
Units: percentage (%)
Description: Total Organic Carbon
http://lod.bco-dmo.org/id/dataset-parameter/810936.rdf
Name: TON
Units: percentage (%)
Description: Total Organic Nitrogen - bd = below detection limit
http://lod.bco-dmo.org/id/dataset-parameter/810937.rdf
Name: Total_C_Total_N
Units: percentage (%)
Description: Total Carbon/Total Nitrogen
http://lod.bco-dmo.org/id/dataset-parameter/810938.rdf
Name: Ratio_13C_12C
Units: percentage (%)
Description: Ratio of stable isotopes 13C/12C
http://lod.bco-dmo.org/id/dataset-parameter/810939.rdf
Name: Clay_OH
Units: percentage (%)
Description: wt% OH from clays (150-300°C)
http://lod.bco-dmo.org/id/dataset-parameter/810940.rdf
Name: Observations
Units: unitless
Description: Lithology and alteration observations
http://lod.bco-dmo.org/id/dataset-parameter/810941.rdf
Name: Reference_Picture
Units: unitless
Description: Sample image. See "Data Files" section of metadata for .zip file of images.
http://lod.bco-dmo.org/id/dataset-parameter/813436.rdf
Name: Latitude
Units: decimal degrees
Description: Latitude, south is negative
http://lod.bco-dmo.org/id/dataset-parameter/813438.rdf
Name: Longitude
Units: decimal degrees
Description: Longitude, west is negative
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
666824
https://darchive.mblwhoilibrary.org/bitstream/1912/25947/1/810902_images.zip
download
1471
https://darchive.mblwhoilibrary.org/bitstream/1912/25947/2/dataset-810902_iodp360-chns-analyses__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.810902.1
download
onLine
dataset
<p>Powderized rock material 462 from each sample (produced in the laminar flow hood on the <em>JOIDES</em> Resolution using a sterile mortar and pestle) was immediately transferred to sterile, muffled glass containers and stored in a desiccator until analysis of carbon and nitrogen according to established methods. Briefly, samples were weighed into methanol rinsed silver boats (4 x 6 mm, Costech). 96-well glass plates (combusted 4 hrs at 450°C) holding these samples were placed in a vacuum desiccator that also contained an open dish with about 50 mL fresh, concentrated (12N) HCl. An inverted crystallization dish was placed over the samplesto protect them from water condensation. The desiccator was closed and pumped out with an airdriven aspirator, to a reading of about ~ 0.5 atm and the desiccator was placed in an oven kept between 60 and 65 °C. Acidification was allowed to run for 60 to 72 hours. When acidification was complete, the samples were removed and set in the oven to dry (60-65 °C), and then placed in a vacuum desiccator charged with indicating silica gel (Fisher S162-500, activated by heating above 220 °C for several hours) and pumped down again and dried for about 24 hours.<br />
<br />
&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Samples were then analyzed on a Costech 4010 Elemental Analyzer connected via a Finningan-MAT Conflo-II interface to a DeltaPlus isotope ratio mass spectrometer.</p>
<p>BCO-DMO processing notes:</p>
<ul>
<li>Adjusted column headers to comply with database requirements</li>
<li>Changed , to ; in observations to comply with database requirements</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
DeltaPlus isotope ratio mass spectrometer
DeltaPlus isotope ratio mass spectrometer
PI Supplied Instrument Name: DeltaPlus isotope ratio mass spectrometer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Costech 4010 Elemental Analyzer
Costech 4010 Elemental Analyzer
PI Supplied Instrument Name: Costech 4010 Elemental Analyzer Instrument Name: Costech International Elemental Combustion System (ECS) 4010 Instrument Short Name:Costech ECS 4010 Instrument Description: The ECS 4010 Nitrogen / Protein Analyzer is an elemental combustion analyser for CHNSO elemental analysis and Nitrogen / Protein determination. The GC oven and separation column have a temperature range of 30-110 degC, with control of +/- 0.1 degC.
Cruise: IODP-360
IODP-360
R/V JOIDES Resolution
Community Standard Description
International Council for the Exploration of the Sea
R/V JOIDES Resolution
vessel
IODP-360
Henry J.B. Dick
Woods Hole Oceanographic Institution
http://publications.iodp.org/scientific_prospectus/360/index.html
Report describing IODP-360
R/V JOIDES Resolution
Community Standard Description
International Council for the Exploration of the Sea
R/V JOIDES Resolution
vessel