http://lod.bco-dmo.org/id/dataset/811896
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-05-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Imaging pulse amplitude modulator fluorometer data collected from Acropora cervicornis under different pH and temperature treatments from experiments at Summerland Key, Florida from July to September 2017
2020-05-19
publication
2020-05-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-05-20
publication
https://doi.org/10.26008/1912/bco-dmo.811896.1
Erinn M. Muller
Mote Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Muller, E. (2020) Imaging pulse amplitude modulator fluorometer data collected from Acropora cervicornis under different pH and temperature treatments from experiments at Summerland Key, Florida from July to September 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-05-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.811896.1 [access date]
Dataset Description: <p>Imaging pulse amplitude modulator fluorometer data collected from <em>Acropora cervicornis</em> under different pH and temperature treatments from experiments at Summerland Key, Florida&nbsp;&nbsp;(24.6616,-81.4538) from July to September 2017.</p> Methods and Sampling: <p>Corals from different treatment scenarios were IPAMed every month for the duration of the two-month long experiment. All fragments were subjected to a light curve where the initial pulse represents the max Yield after dark acclimation and the subsequent electron transport rate (ETR) values are recorded after the corals were exposed to increasing light intensities over time.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1452538 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1452538
completed
Erinn M. Muller
Mote Marine Laboratory
941-388-4441 x 310
1600 Ken Thompson Parkway
Sarasota
FL
34241
USA
emuller@mote.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Tank
Genotype
pH
Temp
mYield
mETR
alpha
Imaging pulse amplitude modulator (IPAM) fluorometer
theme
None, User defined
tank
sample type
treatment
photosynthetic rate
Electron Transport Rate (ETR)
alpha
featureType
BCO-DMO Standard Parameters
Fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
https://osprey.bco-dmo.org/project/642850
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
<p><em>NSF Award Abstract:</em><br />
Caribbean staghorn coral was one of the most common corals within reefs of the Florida Keys several decades ago. Over the last 40 years disease, bleaching, overfishing and habitat degradation caused a 95% reduction of the population. Staghorn coral is now listed as threatened under the U.S. Endangered Species Act of 1973. Within the past few years, millions of dollars have been invested for the purpose of restoring the population of staghorn coral within Florida and the U.S. Virgin Islands. Significant effort has been placed on maintaining and propagating corals of known genotypes within coral nurseries for the purpose of outplanting. However, little is known about the individual genotypes that are currently being outplanted from nurseries onto coral reefs. Are the genotypes being used for outplanting resilient enough to survive the three major stressors affecting the population in the Florida Keys: disease, high water temperatures, and ocean acidification? The research within the present study will be the first step in answering this critically important question. The funded project will additionally develop a research-based afterschool program with K-12 students in the Florida Keys and U.S. Virgin Islands that emphasizes an inquiry-based curriculum, STEM research activities, and peer-to-peer mentoring. The information from the present study will help scientists predict the likelihood of species persistence within the lower Florida Keys under future climate-change and ocean-acidification scenarios. Results of this research will also help guide restoration efforts throughout Florida and the Caribbean, and lead to more informative, science-based restoration activities.</p>
<p><em>Acropora cervicornis</em> dominated shallow-water reefs within the Florida Keys for at least the last half a million years, but the population has recently declined due to multiple stressors. Understanding the current population level of resilience to three major threats - disease outbreaks, high water temperatures, and ocean acidification conditions - is critical for the preservation of this threatened species. Results from the present study will answer the primary research question: will representative genotypes from the lower Florida Keys provide enough phenotypic variation for this threatened species to survive in the future? The present proposal will couple controlled laboratory challenge experiments with field data and modeling applications, and collaborate with local educators to fulfill five objectives: 1) identify <em>A. cervicornis</em> genotypes resistant to disease, 2) identify <em>A. cervicornis</em> genotypes resilient to high water temperature and ocean acidification conditions, 3) quantify how high water temperature and ocean acidification conditions impact disease dynamics on <em>A. cervicornis</em>; 4) determine tradeoffs in life-history traits because of resilience factors; and 5) apply a trait-based model, which will predict genotypic structure of a population under different environmental scenarios.</p>
Resilient Acerv
largerWorkCitation
project
eng; USA
oceans
2020-05-19
Florida Keys, Summerland Key, FL 24.563595°, -81.278572°
0
BCO-DMO catalogue of parameters from Imaging pulse amplitude modulator fluorometer data collected from Acropora cervicornis under different pH and temperature treatments from experiments at Summerland Key, Florida from July to September 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/811905.rdf
Name: Tank
Units: unitless
Description: <p>identifies the tank number that held the particular coral fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/811906.rdf
Name: Genotype
Units: unitless
Description: <p>identifies the genotype number of the coral animal for each fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/811907.rdf
Name: pH
Units: unitless
Description: <p>identifies the treatment pH level: Ambient = 8.1 pH; High CO2 = 7.7 pH</p>
http://lod.bco-dmo.org/id/dataset-parameter/811908.rdf
Name: Temp
Units: unitless
Description: <p>identifies the treatment temperature level: High = 31.5C; Ambient = 27C</p>
http://lod.bco-dmo.org/id/dataset-parameter/811909.rdf
Name: mYield
Units: unitless
Description: <p>change in photosynthetic yield between July and September 2017 after dark acclimation (unitless, but represents the proportion of electrons being used for photosynthesis, potential values range from 0 - 1)</p>
http://lod.bco-dmo.org/id/dataset-parameter/811910.rdf
Name: mETR
Units: umol electrons m–2 s–1
Description: <p>change in the maximum level of electron transport rate between July and September 2017</p>
http://lod.bco-dmo.org/id/dataset-parameter/811911.rdf
Name: alpha
Units: change in ETR over increasing values of PAR
Description: <p>change in the slope of the electron transport rate between July and September 2017; units represent the change in ETR (umol electrons m–2 s–1 ) over increasing values of PAR</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
21114
https://darchive.mblwhoilibrary.org/bitstream/1912/25789/1/dataset-811896_imaging-pulse-amplitude-modulator-fluorometer-2017__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.811896.1
download
onLine
dataset
<p>Corals from different treatment scenarios were IPAMed every month for the duration of the two-month long experiment. All fragments were subjected to a light curve where the initial pulse represents the max Yield after dark acclimation and the subsequent electron transport rate (ETR) values are recorded after the corals were exposed to increasing light intensities over time.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>We derived three key parameters from the PAM photophysiology data set: maximum yield (mY), maximum electron transport rate (mETR), and the initial slope of the ETR curve (alpha).</p>
<p>PAM dataset parameters: Maximum electron transport rate (mETR) was estimated using a linear search algorithm that used the slopes between adjacent (PAR, ETR) data points to determine the first local critical point (maximum or plateau). The algorithm first searches for three data points between which the slope changes sign. If no sign change is detected, the algorithm then looks for the largest set of two data points between which the slope is less than a user-defined threshold (default is 0.1). In the former case, the middle point is chosen as the (mETR) coordinate, while in the later case the second endpoint is used. If neither search yields a solution, the threshold is increased by 0.01 and the two linear searches are repeated until a solution is found. The slope alpha is calculated with a similar algorithm that searches all PAR levels x such that min(PAR) = 55 &lt; x &lt; mPAR for pairs of points between which the ratio of the local slope to the initial slope (slope between (0,0) and first non-zero datapoint) is less than a user-defined threshold (default is 0.1). If no solution is found, the threshold is increased by 0.01 and the search is repeated. Once a pair of points <em>i</em> and <em>i+1</em> have been identified, all data points with PAR levels less than that of point <em>i</em> are used to calculate the slope.&nbsp; If the threshold increases to greater than 0.25, the algorithm terminates by returning the slope of all points with PAR &lt; mPAR. Both algorithms return NA when given zero vectors and are both implemented in R.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Imaging pulse amplitude modulator (IPAM) fluorometer
Imaging pulse amplitude modulator (IPAM) fluorometer
PI Supplied Instrument Name: Imaging pulse amplitude modulator (IPAM) fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/