http://lod.bco-dmo.org/id/dataset/812240
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-05-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbon isotopic compositions and fractionation factors of M. jannaschii in high and low hydrogen (H2) environments
2020-05-25
publication
2020-05-25
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-07-09
publication
https://doi.org/10.26008/1912/bco-dmo.812240.1
Susan Q. Lang
University of South Carolina at Columbia
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lang, S. (2020) Carbon isotopic compositions and fractionation factors of M. jannaschii in high and low hydrogen (H2) environments. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-05-25 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.812240.1 [access date]
M. jannaschii high/low H2: isotopes Dataset Description: <p>Carbon isotopic compositions and fractionation factors of amino acids and squalenoid lipids in Methanocaldococcus&nbsp; jannaschii in high and low hydrogen (H2) environments.</p> Methods and Sampling: <p><strong>Isolation and isotopic analysis of squalenoids:</strong><br />
Cell pellets were freeze-dried overnight, ground with a clean spatula, and extracted three times by sonication in a centrifuge tube filled with 50 mL of 3:1 dichloromethane:methanol (DCM:MeOH). All glassware was combusted overnight at 500°C to remove organics prior to use. After sonication, the extracts were spun in a centrifuge at 125 g for 15 min and the supernatant was decanted to a separate vial. All extracts were combined and the solvent was evaporated to dryness in a rotary evaporator.&nbsp; A maximum of 2 mL of 9:1 DCM:MeOH was added to dissolve the total extract that was then passed over Na2SO4 to remove water. The water-free extract was then separated into different fractions over SepraTM NH2 bulk-packing (P/N 1001711653 572122 – U) silica column by eluting with solvents of increasing polarity (F1 = 5 mL of hexane, F2 = 6 mL of 3:1 hexane:DCM, F3 = 7 mL of 9:1 DCM:acetone, F4 = 8 mL of 4% formic acid in DCM). The apolar fraction (F1) was dried under N2, then re-dissolved in 50 µL of hexane for identification.</p>
<p>The lipids in the apolar fraction were identified and quantified using an Agilent Technologies 5975 inert XL Mass Selective Detector after separation on an Agilent J&amp;W GC HP-5MS UI capillary column (30 m × 0.25 mm i.d., 0.25 µm film thickness, P/N 19091S – 433UIE) using He as the carrier gas. Samples were injected in pulse splitless mode. The GC oven was from an initial temperature of 70°C, then heated to 150°C at 15°C per min, then to 300°C at 5°C per min. Peaks were quantified by comparison to a 5-point standard curve of a C7-C30 alkane series (P/N 49451 – U, Sigma Aldrich). The isotopic composition of biomarkers in the apolar fraction was determined on a Thermo Scientific Gas Chromatograph-IsolinkII-Isotope Ratio Mass Spectrometer (GC-IsolinkII-IRMS) equipped with an Agilent DB-5 fused silica column (30 m × 0.25 mm i.d., 0.25 µm film thickness) with He as the carrier gas.</p>
<p><strong>Isolation, characterization, and isotopic analysis of amino acids:</strong><br />
Pelleted cells were hydrolyzed with 6 M HCl (Ultrapure grade) with 1% of 11 mM ascorbic acid under N2 at 110°C for 20 h (Henrichs, 1991). After cooling, hydrolyzed amino acids were spiked with internal standard norvaline and derivatized with acidified isopropanol and acetyl chloride for 1 h at 110°C (Silfer et al., 1991). The samples then reacted at 110°C for 1 h on a hot plate. They were then esterified with trifluoroacetic anhydride (TFAA) for 10 min at 110°C for 10 min. The resulting derivatives were dissolved in dichloromethane. The isotopic signatures of derivatized amino acids were determined by GC-IsolinkII-IRMS</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0939564 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0939564
completed
Susan Q. Lang
University of South Carolina at Columbia
803-777-8832
School of the Earth Ocean and Environment 701 Sumter St. EWS 617
Columbia
SC
29208
USA
slang@geol.sc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Experiment
Culture_ID
d13C_ppt_Ala
d13C_ppt_Gly
d13C_ppt_Thr
d13C_ppt_Ser
d13C_ppt_Val
d13C_ppt_Leu
d13C_ppt_Iso
d13C_ppt_Pro
d13C_ppt_Glu
d13C_ppt_Phe
Weighted_Avg_d13C_THAA_ppt
d13C_ppt_Sq_3
d13C_ppt_Sq_4
d13C_ppt_Sq_5
d13C_ppt_Sq_6
Weighted_Avg_ppt_d13C_Sq
DIC_mM_To
DIC_mM_Tf
TFAA_uM_To
TFAA_uM_Tf
THAA_uM_To
THAA_uM_Tf
Fractionation_factor_ppt_eCO2_CH4
Fractionation_factor_ppt_eCO2_B
Fractionation_factor_ppt_eCO2_AA
Fractionation_factor_ppt_eCO2_squalenoids
Gas Chromatograph Mass Spectrometer
Thermo Scientific Trace 1310 Gas Chromatograph-IsolinkII-Delta V Isotope Ratio Mass Spectrometer
Thermo Scientific GasBench- Delta V Isotope Radio Mass Spectrometer
theme
None, User defined
treatment
sample identification
amino acid concentration
lipids in biota
dissolved inorganic Carbon
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Gas Chromatograph Mass Spectrometer
Gas Chromatograph Mass Spectrometer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Center for Dark Energy Biosphere Investigations
http://www.darkenergybiosphere.org
Center for Dark Energy Biosphere Investigations
The mission of the Center for Dark Energy Biosphere Investigations (C-DEBI) is to explore life beneath the seafloor and make transformative discoveries that advance science, benefit society, and inspire people of all ages and origins.
C-DEBI provides a framework for a large, multi-disciplinary group of scientists to pursue fundamental questions about life deep in the sub-surface environment of Earth. The fundamental science questions of C-DEBI involve exploration and discovery, uncovering the processes that constrain the sub-surface biosphere below the oceans, and implications to the Earth system. What type of life exists in this deep biosphere, how much, and how is it distributed and dispersed? What are the physical-chemical conditions that promote or limit life? What are the important oxidation-reduction processes and are they unique or important to humankind? How does this biosphere influence global energy and material cycles, particularly the carbon cycle? Finally, can we discern how such life evolved in geological settings beneath the ocean floor, and how this might relate to ideas about the origin of life on our planet?
C-DEBI's scientific goals are pursued with a combination of approaches:
(1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites;
(2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems;
(3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and
(4) entrain, educate, inspire, and mentor an interdisciplinary community of researchers and educators, with an emphasis on undergraduate and graduate students and early-career scientists.
Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI.
Data Management:
C-DEBI is committed to ensuring all the data generated are publically available and deposited in a data repository for long-term storage as stated in their Data Management Plan (PDF) and in compliance with the NSF Ocean Sciences Sample and Data Policy. The data types and products resulting from C-DEBI-supported research include a wide variety of geophysical, geological, geochemical, and biological information, in addition to education and outreach materials, technical documents, and samples. All data and information generated by C-DEBI-supported research projects are required to be made publically available either following publication of research results or within two (2) years of data generation.
To ensure preservation and dissemination of the diverse data-types generated, C-DEBI researchers are working with BCO-DMO Data Managers make data publicly available online. The partnership with BCO-DMO helps ensure that the C-DEBI data are discoverable and available for reuse. Some C-DEBI data is better served by specialized repositories (NCBI's GenBank for sequence data, for example) and, in those cases, BCO-DMO provides dataset documentation (metadata) that includes links to those external repositories.
C-DEBI
largerWorkCitation
program
Bioenergetic influences upon carbon flow in alkaliphilic sulfate-reducing microbial populations with relevance to the subsurface biosphere at the Lost City Hydrothermal Field
https://osprey.bco-dmo.org/project/661802
Bioenergetic influences upon carbon flow in alkaliphilic sulfate-reducing microbial populations with relevance to the subsurface biosphere at the Lost City Hydrothermal Field
<p>Project description from <a href="http://www.darkenergybiosphere.org/research-activities/research-support/funded-projects/" target="_blank">C-DEBI</a>:<br />
The microbial biosphere in serpentinizing subseafloor rocks is globally significant. Tantalizing evidence from studies of the Lost City Hydrothermal Field and continental ophiolites indicates that hydrogendriven microbial metabolisms prevails under the highly reducing, high pH conditions that characterize these environments. Interest in these processes is evident from an upcoming cruise to the Atlantis Massif in Fall 2015 to obtain drill cores in the vicinity of the Lost City Hydrothermal Field (IODP Expedition #357; both PIs were proponents of the IODP proposal and have applied as shipboard scientists). The PIs and colleagues have made headway over the last decade in identifying the key organisms and metabolisms present at the LCHF, and in constraining the sources and fates of carbon compounds. The linkages between geology and biology remain enigmatic, however, because of the precipitation of inorganic carbon at high pHs and overlapping biogenic and abiogenic carbon sources. We propose here to investigate the influence of free energy availability by sulfate reduction in resource utilization and carbon flow by model alkaliphilic prokaryotes. The laboratory approach using a model system will inform shipboard experiments with fresh samples from the AM, and the potential characterization of new organisms from serpentinizing terrains.</p>
<p>This project was funded by a <a href="http://www.darkenergybiosphere.org/research-activities/research-support/research-grants/" target="_blank">C-DEBI Research Grant</a></p>
Carbon flow through SRB
largerWorkCitation
project
eng; USA
oceans
-42.11667
-42.11667
30.11667
30.11667
2020-05-25
Atlantis Massif, 30 8'N, 42 8'W
0
BCO-DMO catalogue of parameters from Carbon isotopic compositions and fractionation factors of M. jannaschii in high and low hydrogen (H2) environments
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/812634.rdf
Name: Experiment
Units: unitless
Description: <p>Experiment description: either high (abundant) or low (limited) hydrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/812635.rdf
Name: Culture_ID
Units: unitless
Description: <p>culture identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/812636.rdf
Name: d13C_ppt_Ala
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of alanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/812637.rdf
Name: d13C_ppt_Gly
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of glycine</p>
http://lod.bco-dmo.org/id/dataset-parameter/812638.rdf
Name: d13C_ppt_Thr
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of threonine</p>
http://lod.bco-dmo.org/id/dataset-parameter/812639.rdf
Name: d13C_ppt_Ser
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of serine</p>
http://lod.bco-dmo.org/id/dataset-parameter/812640.rdf
Name: d13C_ppt_Val
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of valine</p>
http://lod.bco-dmo.org/id/dataset-parameter/812641.rdf
Name: d13C_ppt_Leu
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of leucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/812642.rdf
Name: d13C_ppt_Iso
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of isoleucine</p>
http://lod.bco-dmo.org/id/dataset-parameter/812643.rdf
Name: d13C_ppt_Pro
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of proline</p>
http://lod.bco-dmo.org/id/dataset-parameter/812644.rdf
Name: d13C_ppt_Glu
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of glu</p>
http://lod.bco-dmo.org/id/dataset-parameter/812645.rdf
Name: d13C_ppt_Phe
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of phenylalanine</p>
http://lod.bco-dmo.org/id/dataset-parameter/812646.rdf
Name: Weighted_Avg_d13C_THAA_ppt
Units: parts per thousand (ppt)
Description: <p>Weighted isotopic ratio of each amino acid as a toal</p>
http://lod.bco-dmo.org/id/dataset-parameter/812647.rdf
Name: d13C_ppt_Sq_3
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of squalenoid with three double bonds</p>
http://lod.bco-dmo.org/id/dataset-parameter/812648.rdf
Name: d13C_ppt_Sq_4
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of squalenoid with four double bonds</p>
http://lod.bco-dmo.org/id/dataset-parameter/812649.rdf
Name: d13C_ppt_Sq_5
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of squalenoid with five double bonds</p>
http://lod.bco-dmo.org/id/dataset-parameter/812650.rdf
Name: d13C_ppt_Sq_6
Units: parts per thousand (ppt)
Description: <p>13C isotopic ratio of squalene</p>
http://lod.bco-dmo.org/id/dataset-parameter/812651.rdf
Name: Weighted_Avg_ppt_d13C_Sq
Units: parts per thousand (ppt)
Description: <p>weighted average of the isotopic composition of squalenoids</p>
http://lod.bco-dmo.org/id/dataset-parameter/812652.rdf
Name: DIC_mM_To
Units: milliMolar (mM)
Description: <p>Concentration of dissolved inorganic carbon at the start of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/812653.rdf
Name: DIC_mM_Tf
Units: milliMolar (mM)
Description: <p>Concentration of dissolved inorganic carbon at the end of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/812654.rdf
Name: TFAA_uM_To
Units: microMolar (uM)
Description: <p>Concentration of total free amino acids at the start of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/812655.rdf
Name: TFAA_uM_Tf
Units: microMolar (uM)
Description: <p>Concentration of total free amino acids at the end of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/812656.rdf
Name: THAA_uM_To
Units: microMolar (uM)
Description: <p>Concentration of total hydrolizable amino acids at the start of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/812657.rdf
Name: THAA_uM_Tf
Units: microMolar (uM)
Description: <p>Concentration of total hydrolizable amino acids at the end of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/812658.rdf
Name: Fractionation_factor_ppt_eCO2_CH4
Units: parts per thousand (ppt)
Description: <p>Fractionation factor between CO2-and methane</p>
http://lod.bco-dmo.org/id/dataset-parameter/812659.rdf
Name: Fractionation_factor_ppt_eCO2_B
Units: parts per thousand (ppt)
Description: <p>Fractionation factor between CO2-and biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/812660.rdf
Name: Fractionation_factor_ppt_eCO2_AA
Units: parts per thousand (ppt)
Description: <p>Fractionation factor between CO2-and amino acids</p>
http://lod.bco-dmo.org/id/dataset-parameter/812661.rdf
Name: Fractionation_factor_ppt_eCO2_squalenoids
Units: parts per thousand (ppt)
Description: <p>Fractionation factor between CO2-and squalenoids</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1323
https://darchive.mblwhoilibrary.org/bitstream/1912/25953/1/dataset-812240_m-jannaschii-highlow-h2-isotopes-and-fractionation-factors__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.812240.1
download
onLine
dataset
<p><strong>Isolation and isotopic analysis of squalenoids:</strong><br />
Cell pellets were freeze-dried overnight, ground with a clean spatula, and extracted three times by sonication in a centrifuge tube filled with 50 mL of 3:1 dichloromethane:methanol (DCM:MeOH). All glassware was combusted overnight at 500°C to remove organics prior to use. After sonication, the extracts were spun in a centrifuge at 125 g for 15 min and the supernatant was decanted to a separate vial. All extracts were combined and the solvent was evaporated to dryness in a rotary evaporator.&nbsp; A maximum of 2 mL of 9:1 DCM:MeOH was added to dissolve the total extract that was then passed over Na2SO4 to remove water. The water-free extract was then separated into different fractions over SepraTM NH2 bulk-packing (P/N 1001711653 572122 – U) silica column by eluting with solvents of increasing polarity (F1 = 5 mL of hexane, F2 = 6 mL of 3:1 hexane:DCM, F3 = 7 mL of 9:1 DCM:acetone, F4 = 8 mL of 4% formic acid in DCM). The apolar fraction (F1) was dried under N2, then re-dissolved in 50 µL of hexane for identification.</p>
<p>The lipids in the apolar fraction were identified and quantified using an Agilent Technologies 5975 inert XL Mass Selective Detector after separation on an Agilent J&amp;W GC HP-5MS UI capillary column (30 m × 0.25 mm i.d., 0.25 µm film thickness, P/N 19091S – 433UIE) using He as the carrier gas. Samples were injected in pulse splitless mode. The GC oven was from an initial temperature of 70°C, then heated to 150°C at 15°C per min, then to 300°C at 5°C per min. Peaks were quantified by comparison to a 5-point standard curve of a C7-C30 alkane series (P/N 49451 – U, Sigma Aldrich). The isotopic composition of biomarkers in the apolar fraction was determined on a Thermo Scientific Gas Chromatograph-IsolinkII-Isotope Ratio Mass Spectrometer (GC-IsolinkII-IRMS) equipped with an Agilent DB-5 fused silica column (30 m × 0.25 mm i.d., 0.25 µm film thickness) with He as the carrier gas.</p>
<p><strong>Isolation, characterization, and isotopic analysis of amino acids:</strong><br />
Pelleted cells were hydrolyzed with 6 M HCl (Ultrapure grade) with 1% of 11 mM ascorbic acid under N2 at 110°C for 20 h (Henrichs, 1991). After cooling, hydrolyzed amino acids were spiked with internal standard norvaline and derivatized with acidified isopropanol and acetyl chloride for 1 h at 110°C (Silfer et al., 1991). The samples then reacted at 110°C for 1 h on a hot plate. They were then esterified with trifluoroacetic anhydride (TFAA) for 10 min at 110°C for 10 min. The resulting derivatives were dissolved in dichloromethane. The isotopic signatures of derivatized amino acids were determined by GC-IsolinkII-IRMS</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Gas Chromatograph Mass Spectrometer
Gas Chromatograph Mass Spectrometer
PI Supplied Instrument Name: Gas Chromatograph Mass Spectrometer PI Supplied Instrument Description:For or identification and abundance of carbon isotopes. Agilent Technologies 5975 inert XL Mass Selective Detector after separation on an Agilent J&W GC HP-5MS UI capillary column (30 m × 0.25 mm i.d., 0.25 µm film thickness, P/N 19091S – 433UIE) Instrument Name: Gas Chromatograph Mass Spectrometer Instrument Short Name: Instrument Description: Instruments separating gases, volatile substances or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay by a mass spectrometer.
Thermo Scientific Trace 1310 Gas Chromatograph-IsolinkII-Delta V Isotope Ratio Mass Spectrometer
Thermo Scientific Trace 1310 Gas Chromatograph-IsolinkII-Delta V Isotope Ratio Mass Spectrometer
PI Supplied Instrument Name: Thermo Scientific Trace 1310 Gas Chromatograph-IsolinkII-Delta V Isotope Ratio Mass Spectrometer PI Supplied Instrument Description:For measuring isotopic composition of biomarkers, including amino acid and lipids: Thermo Scientific Trace 1310 Gas Chromatograph-IsolinkII-Delta V Isotope Ratio Mass Spectrometer equipped with an Agilent DB-5 fused silica column (30 m × 0.25 mm i.d., 0.25 µm film thickness) and a Gerstel CIS – 6 inlet. Instrument Name: Gas Chromatograph Mass Spectrometer Instrument Short Name: Instrument Description: Instruments separating gases, volatile substances or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay by a mass spectrometer.
Thermo Scientific GasBench- Delta V Isotope Radio Mass Spectrometer
Thermo Scientific GasBench- Delta V Isotope Radio Mass Spectrometer
PI Supplied Instrument Name: Thermo Scientific GasBench- Delta V Isotope Radio Mass Spectrometer PI Supplied Instrument Description:For measuring isotopic composition of dissolved inorganic carbon. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/