http://lod.bco-dmo.org/id/dataset/816216
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-06-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
HPLC pigment data from samples collected during R/V Savannah cruises conducted in the South Atlantic Bight off the coast of Georgia from 2015-2017
2020-06-19
publication
2020-06-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-06-23
publication
https://doi.org/10.26008/1912/bco-dmo.816216.1
Peter Lee
College of Charleston - Hollings Marine Lab
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lee, P. (2020) HPLC pigment data from samples collected during R/V Savannah cruises conducted in the South Atlantic Bight off the coast of Georgia from 2015-2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-06-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.816216.1 [access date]
HPLC pigment data from samples collected during SAV cruises 2015-2017 Dataset Description: <p>HPLC pigment data collected during several R/V Savannah cruises conducted from 2015 to 2017 along a transect from shelf waters to oligotrophic waters in the South Atlantic Bight off the coast of Georgia (Navy Op Area NA06).</p> Methods and Sampling: <p>Algal HPLC samples were collected by filtration under low vacuum through GF/F filters and frozen at -80C for on-shore analysis. Samples were extracted in acetone and analyzed using an Agilent 1100 HPLC system equipped with autosampler, photodiode array, and fluorescence detectors. The gradient elution program utilized was a slight modification of the Zapata et al. method (2000). Complete details of the HPLC method are described in DiTullio and Geesey 2003.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1436458 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1436458
completed
Peter Lee
College of Charleston - Hollings Marine Lab
843-460-9785
331 Fort Johnson Rd.
Charleston
SC
29412
USA
leep@cofc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise_ID
Station
UTC_Date
UTC_Time
ISO_DateTime_UTC
Latitude
Longitude
Bottom_Depth
CTD_bottle
Depth
Chl_c3
Chl_ide
MgDVP
Chl_c2
Chl_c1
Peridinin
But_19
Fuco
Neo
Prasino
Viola
Hex_19
DD
cis_fuco
Allo
Diato
Monad
Zeax
Lutein
Croco
Chl_b
Chl_c2_like
Chl_a_like
DV_Chl_a
Chl_a
Ph_tin
a_Car
b_Car
Sea-Bird Scientific SBE 911 CTD carousel
High Performance Liquid Chromatograph (HPLC) Agilent 1100
Niskin bottle
SBE-25 CTD
theme
None, User defined
Cruise identfier
station
date
time of day
ISO_DateTime_UTC
latitude
longitude
depth_w
bottle
depth
chlorophyll c3
chlorophyllide
mg_dvp_a5
chlorophyll c2
chlorophyll c1
peridinin
19-prime-butanoyloxyfucoxanthin
fucoxanthin
neoxanthin
prasinoxanthin
violaxanthin
19-prime-hexanoyloxyfucoxanthin
diadinoxanthin
cis-fucoxanthin
alloxanthin
diatoxanthin
monad
zeaxanthin
lutein
crocoxanthin pigment
chlorophyll b
pigment concentration
divinyl chlorophyll a
chlorophyll a
p_phytin_a
carotene-alpha
carotene-beta
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird 911
High-Performance Liquid Chromatograph
Niskin bottle
Sea-Bird SBE 25 Sealogger CTD
instrument
BCO-DMO Standard Instruments
SAV-15-04
SAV-15-16
SAV-15-20
SAV-15-26
SAV-16-06
SAV-16-22
SAV-16-28
SAV-17-02
service
Deployment Activity
South Atlantic Bight off the coast of Georgia
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Vitamin B12 and nitrogen regulation of oceanic dimethylsulfoniopropionate and dimethylsulfide
https://osprey.bco-dmo.org/project/516651
RUI: Vitamin B12 and nitrogen regulation of oceanic dimethylsulfoniopropionate and dimethylsulfide
<p><em>Description from NSF award abstract:</em><br />
Vitamin B12 and nitrogen are nutrients critical to phytoplankton growth. Since B12 is produced solely by bacteria, phytoplankton must acquire their B12 from bacteria. Nitrogen is used to produce the amino acid methionine and B12 is required by the enzymes that form methionine. Methionine is the precursor to the algal metabolite dimethylsulfoniopropionate (DMSP). Bacteria degrade this compound to the climatically-active compound dimethylsulfide (DMS). Subsequent DMS transfer into the atmosphere is considered a significant driver of cloud formation and a possible climate feedback mechanism. DMSP can also be degraded via a secondary pathway to form methylmercaptopropionate (MMPA), which is not released to the atmosphere. Consequently, DMSP formation and the extent of DMSP degradation to DMS or MMPA are susceptible to B12 availability. Nitrogen availability influences this effect by controlling methionine production. Thus, the overarching premise for this study is that B12 availability regulates oceanic DMSP and DMS formation, and is synergistically impacted by nitrogen limitation. By providing a mechanistic understanding of relevant biogeochemical parameters this study will significantly improve the incorporation of sulfur-related microbial processes into climate models.</p>
<p>This project will combine established biogeochemistry-based measurements with cutting-edge metabolomics, transcriptomics and proteomics techniques in laboratory and field studies. Culture experiments will examine the interactive effect of B12 and nitrogen availability on DMSP formation in several ecologically-relevant phytoplankton taxa. Second, the microbial degradation of DMSP and DMS in relation to B12 availability will be examined using several environmentally-important bacteria and archaea. Finally, field studies will examine the seasonal variability of B12, DMSP and DMS, and the relative importance of DMS and MMPA formation in the South Atlantic Bight. Gene and protein expression will be assessed at each level of this study to identify gene products, metabolic pathways, and cellular mechanisms underlying the interconnections between B12, sulfur, and nitrogen cycles. The results generated will have a major impact on current understanding of the role of B12 and nitrogen on the DMSP and DMS cycling, as well as the potential role of these stressors in global climate change. In addition to providing evidence for microbe-based mechanisms behind the modulation of oceanic DMS, this project will (1) furnish an explanation for "summer DMS paradox", thus having significant implications for the development of future DMS models, (2) assess the interactive impact of B12 and nitrogen availability on intracellular DMSP production and (3) provide insight as to whether B12 may play a far more critical role in modulating climate feedback mechanisms on phytoplankton productivity.</p>
B12 Impacts on DMSP
largerWorkCitation
project
eng; USA
oceans
South Atlantic Bight off the coast of Georgia
-81.121513
-76.343633
31.031829
31.42113
2015-03-16
2017-01-27
North Atlantic Ocean in the South Atlantic Bight off the coast of Georgia; Navy Op Area NA06
0
BCO-DMO catalogue of parameters from HPLC pigment data from samples collected during R/V Savannah cruises conducted in the South Atlantic Bight off the coast of Georgia from 2015-2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/816236.rdf
Name: Cruise_ID
Units: unitless
Description: <p>Cruise Identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/816237.rdf
Name: Station
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/816238.rdf
Name: UTC_Date
Units: unitless
Description: <p>Start date for sample collection; Coordinated Universal Time; Format: MM/DD/YYYY</p>
http://lod.bco-dmo.org/id/dataset-parameter/816239.rdf
Name: UTC_Time
Units: unitless
Description: <p>Start time for sample collection; Coordinated Universal Time; Format: hh:mm:ss</p>
http://lod.bco-dmo.org/id/dataset-parameter/816240.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Start date and time of sample collection formatted to ISO8601 standard: YYYY-MM-DDThh:mm:ssZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/816241.rdf
Name: Latitude
Units: decimal degrees North
Description: <p>Start latitude for sample collection; Negative = South</p>
http://lod.bco-dmo.org/id/dataset-parameter/816242.rdf
Name: Longitude
Units: decimal degrees East
Description: <p>Start longitude for sample collection; Negative = West</p>
http://lod.bco-dmo.org/id/dataset-parameter/816243.rdf
Name: Bottom_Depth
Units: meters (m)
Description: <p>Bottom depth from ship's sonar</p>
http://lod.bco-dmo.org/id/dataset-parameter/816244.rdf
Name: CTD_bottle
Units: unitless
Description: <p>Niskin bottle number from CTD rosette</p>
http://lod.bco-dmo.org/id/dataset-parameter/816245.rdf
Name: Depth
Units: meters (m)
Description: <p>Sample depth from CTD readout</p>
http://lod.bco-dmo.org/id/dataset-parameter/816246.rdf
Name: Chl_c3
Units: nanograms per liter (ng/L)
Description: <p>Chlorophyll c3 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816247.rdf
Name: Chl_ide
Units: ng/L
Description: <p>Chlorophyllide concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816248.rdf
Name: MgDVP
Units: ng/L
Description: <p>Magnesium-2,4-divinyl phaeoporphyrin a5 monomethyl ester concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816249.rdf
Name: Chl_c2
Units: ng/L
Description: <p>Chlorophyll c2 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816250.rdf
Name: Chl_c1
Units: ng/L
Description: <p>Chlorophyll c1 concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816251.rdf
Name: Peridinin
Units: ng/L
Description: <p>Peridinin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816252.rdf
Name: But_19
Units: ng/L
Description: <p>19-prime butanoyloxyfucoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816253.rdf
Name: Fuco
Units: ng/L
Description: <p>Fucoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816254.rdf
Name: Neo
Units: ng/L
Description: <p>Neoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816255.rdf
Name: Prasino
Units: ng/L
Description: <p>Prasinoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816256.rdf
Name: Viola
Units: ng/L
Description: <p>Violaxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816257.rdf
Name: Hex_19
Units: ng/L
Description: <p>19-prime hexanoyloxyfucoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816258.rdf
Name: DD
Units: ng/L
Description: <p>Diadinoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816259.rdf
Name: cis_fuco
Units: ng/L
Description: <p>Cis-fucoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816260.rdf
Name: Allo
Units: ng/L
Description: <p>Alloxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816261.rdf
Name: Diato
Units: ng/L
Description: <p>Diatoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816262.rdf
Name: Monad
Units: ng/L
Description: <p>Monadoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816263.rdf
Name: Zeax
Units: ng/L
Description: <p>Zeaxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816264.rdf
Name: Lutein
Units: ng/L
Description: <p>Lutein concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816265.rdf
Name: Croco
Units: ng/L
Description: <p>Crocoxanthin concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816266.rdf
Name: Chl_b
Units: ng/L
Description: <p>Chlorophyll b concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816267.rdf
Name: Chl_c2_like
Units: ng/L
Description: <p>Chlorophyll c2-like concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816268.rdf
Name: Chl_a_like
Units: ng/L
Description: <p>Chlorophyll a-like concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816269.rdf
Name: DV_Chl_a
Units: ng/L
Description: <p>Divinyl-chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816270.rdf
Name: Chl_a
Units: ng/L
Description: <p>Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816271.rdf
Name: Ph_tin
Units: ng/L
Description: <p>Pheophytin a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816272.rdf
Name: a_Car
Units: ng/L
Description: <p>Carotene-alpha concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816273.rdf
Name: b_Car
Units: ng/L
Description: <p>Carotene-beta concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
159768
https://darchive.mblwhoilibrary.org/bitstream/1912/25889/1/dataset-816216_hplc-pigments__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.816216.1
download
onLine
dataset
<p>Algal HPLC samples were collected by filtration under low vacuum through GF/F filters and frozen at -80C for on-shore analysis. Samples were extracted in acetone and analyzed using an Agilent 1100 HPLC system equipped with autosampler, photodiode array, and fluorescence detectors. The gradient elution program utilized was a slight modification of the Zapata et al. method (2000). Complete details of the HPLC method are described in DiTullio and Geesey 2003.</p>
Specified by the Principal Investigator(s)
<p>Pigment concentrations were determined using standard peak integration procedures with Agilent's ChemStation (version B.03.02), and entered into Microsoft Excel spreadsheets for submission to BCO-DMO. Pigment concentrations (ng/L) reported are: chlorophyll c3, chlorophyllide, magnesium-2,4-divinyl phaeoporphyrin a5 monomethyl ester, chlorophyll c2, chlorophyll c1, peridinin, 19-prime butanoyloxyfucoxanthin, fucoxanthin, neoxanthin, prasinoxanthin, violaxanthin, 19-prime hexanoyloxyfucoxanthin, diadinoxanthin, cis-fucoxanthin, alloxanthin, diatoxanthin, monadoxanthin, zeaxanthin, lutein, crocoxanthin, chlorophyll b, chlorophyll c2-like, chlorophyll a-like, divinyl chlorophyll a, chlorophyll a, pheophytin a, carotene-alpha and carotene-beta.</p>
<p>BCO-DMO Processing:<br />
- renamed fields;<br />
- added date/time field in ISO8601 format.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Sea-Bird Scientific SBE 911 CTD carousel
Sea-Bird Scientific SBE 911 CTD carousel
PI Supplied Instrument Name: Sea-Bird Scientific SBE 911 CTD carousel Instrument Name: CTD Sea-Bird 911 Instrument Short Name:CTD SBE 911 Instrument Description: The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/
High Performance Liquid Chromatograph (HPLC) Agilent 1100
High Performance Liquid Chromatograph (HPLC) Agilent 1100
PI Supplied Instrument Name: High Performance Liquid Chromatograph (HPLC) Agilent 1100 PI Supplied Instrument Description:High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array, and fluorescence detectors. Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Niskin bottle
Niskin bottle
PI Supplied Instrument Name: Niskin bottle Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
SBE-25 CTD
SBE-25 CTD
PI Supplied Instrument Name: SBE-25 CTD Instrument Name: Sea-Bird SBE 25 Sealogger CTD Instrument Short Name:CTD SBE 25 Instrument Description: The Sea-Bird SBE 25 SEALOGGER CTD is battery powered and is typically used to record data in memory, eliminating the need for a large vessel, electrical sea cable, and on-board computer. All SBE 25s can also operate in real-time, transmitting data via an opto-isolated RS-232 serial port. Temperature and conductivity are measured by the SBE 3F Temperature sensor and SBE 4 Conductivity sensor (same as those used on the premium SBE 9plus CTD). The SBE 25 also includes the SBE 5P (plastic) or 5T (titanium) Submersible Pump and TC Duct. The pump-controlled, TC-ducted flow configuration significantly reduces salinity spiking caused by ship heave, and in calm waters allows slower descent rates for improved resolution of water column features. Pressure is measured by the modular SBE 29 Temperature Compensated Strain-Gauge Pressure sensor (available in eight depth ranges to suit the operating depth requirement). The SBE 25's modular design makes it easy to configure in the field for a wide range of auxiliary sensors, including optional dissolved oxygen (SBE 43), pH (SBE 18 or SBE 27), fluorescence, transmissivity, PAR, and optical backscatter sensors. More information from Sea-Bird Electronics: http:www.seabird.com. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0040/
Cruise: SAV-15-04
SAV-15-04
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-04
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-15-16
SAV-15-16
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-16
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-15-20
SAV-15-20
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-20
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-15-26
SAV-15-26
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-26
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-16-06
SAV-16-06
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-06
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-16-22
SAV-16-22
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-22
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-16-28
SAV-16-28
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-28
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-17-02
SAV-17-02
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-02
Peter Lee
College of Charleston - Hollings Marine Lab
R/V Savannah
Community Standard Description
R/V Savannah
vessel