http://lod.bco-dmo.org/id/dataset/816278
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-06-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Size-fractionated chlorophyll-a data from samples collected during R/V Savannah cruises conducted in the South Atlantic Bight off the coast of Georgia from 2015-2017
2020-06-19
publication
2020-06-19
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-06-23
publication
https://doi.org/10.26008/1912/bco-dmo.816278.1
Peter Lee
College of Charleston - Hollings Marine Lab
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lee, P. (2020) Size-fractionated chlorophyll-a data from samples collected during R/V Savannah cruises conducted in the South Atlantic Bight off the coast of Georgia from 2015-2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-06-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.816278.1 [access date]
Size-fractionated chlorophyll-a data from samples collected during SAV cruises 2015-2017 Dataset Description: <p>Size-fractionated chlorophyll-a data collected during several R/V Savannah cruises conducted from 2015 to 2017 along a transect from shelf waters to oligotrophic waters in the South Atlantic Bight off the coast of Georgia (Navy Op Area NA06).</p> Methods and Sampling: <p>All samples were pre-screened through a 202 µm Nitex mesh. Size-fractionated Chlorophyll a (Chl-a) samples were collected by passing discrete 1 L aliquots of seawater through Nitex with mesh sizes of 63 and 20 µm, and collecting the filtrate. The filtrate was then filtered under low vacuum through GF/F (Sterlitech) filters. An additional sample was directly filtered onto a GF/F filter for total Chl-a. The filters were extracted in 90% acetone overnight at -20°C and analyzed according to the protocol described by Welschmeyer (1994). The size classes were calculated by difference (total, &gt; 63 µm, 63-20 µm, &lt; 20 µm). nd = no data</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1436458 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1436458
completed
Peter Lee
College of Charleston - Hollings Marine Lab
843-460-9785
331 Fort Johnson Rd.
Charleston
SC
29412
USA
leep@cofc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise_ID
Station_ID
UTC_Date
UTC_Time
ISO_DateTime_UTC
Latitude
Longitude
Bottom_Depth
Depth
Total_Chl_a
gt_63_um
bw_63_20_um
lt_20_um
Sea-Bird Scientific SBE 911 CTD carousel
Niskin bottle
SBE-25 CTD
Turner Designs 10AU Fluorometer
theme
None, User defined
Cruise identfier
station
date
time of day
ISO_DateTime_UTC
latitude
longitude
depth_w
depth
sum of chlorophyll a like compounds
chlorophyll-a; fluorometric method
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird 911
Niskin bottle
Sea-Bird SBE 25 Sealogger CTD
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
SAV-15-04
SAV-15-16
SAV-15-20
SAV-15-26
SAV-16-06
SAV-16-22
SAV-16-28
SAV-17-02
service
Deployment Activity
South Atlantic Bight off the coast of Georgia
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RUI: Vitamin B12 and nitrogen regulation of oceanic dimethylsulfoniopropionate and dimethylsulfide
https://osprey.bco-dmo.org/project/516651
RUI: Vitamin B12 and nitrogen regulation of oceanic dimethylsulfoniopropionate and dimethylsulfide
<p><em>Description from NSF award abstract:</em><br />
Vitamin B12 and nitrogen are nutrients critical to phytoplankton growth. Since B12 is produced solely by bacteria, phytoplankton must acquire their B12 from bacteria. Nitrogen is used to produce the amino acid methionine and B12 is required by the enzymes that form methionine. Methionine is the precursor to the algal metabolite dimethylsulfoniopropionate (DMSP). Bacteria degrade this compound to the climatically-active compound dimethylsulfide (DMS). Subsequent DMS transfer into the atmosphere is considered a significant driver of cloud formation and a possible climate feedback mechanism. DMSP can also be degraded via a secondary pathway to form methylmercaptopropionate (MMPA), which is not released to the atmosphere. Consequently, DMSP formation and the extent of DMSP degradation to DMS or MMPA are susceptible to B12 availability. Nitrogen availability influences this effect by controlling methionine production. Thus, the overarching premise for this study is that B12 availability regulates oceanic DMSP and DMS formation, and is synergistically impacted by nitrogen limitation. By providing a mechanistic understanding of relevant biogeochemical parameters this study will significantly improve the incorporation of sulfur-related microbial processes into climate models.</p>
<p>This project will combine established biogeochemistry-based measurements with cutting-edge metabolomics, transcriptomics and proteomics techniques in laboratory and field studies. Culture experiments will examine the interactive effect of B12 and nitrogen availability on DMSP formation in several ecologically-relevant phytoplankton taxa. Second, the microbial degradation of DMSP and DMS in relation to B12 availability will be examined using several environmentally-important bacteria and archaea. Finally, field studies will examine the seasonal variability of B12, DMSP and DMS, and the relative importance of DMS and MMPA formation in the South Atlantic Bight. Gene and protein expression will be assessed at each level of this study to identify gene products, metabolic pathways, and cellular mechanisms underlying the interconnections between B12, sulfur, and nitrogen cycles. The results generated will have a major impact on current understanding of the role of B12 and nitrogen on the DMSP and DMS cycling, as well as the potential role of these stressors in global climate change. In addition to providing evidence for microbe-based mechanisms behind the modulation of oceanic DMS, this project will (1) furnish an explanation for "summer DMS paradox", thus having significant implications for the development of future DMS models, (2) assess the interactive impact of B12 and nitrogen availability on intracellular DMSP production and (3) provide insight as to whether B12 may play a far more critical role in modulating climate feedback mechanisms on phytoplankton productivity.</p>
B12 Impacts on DMSP
largerWorkCitation
project
eng; USA
oceans
South Atlantic Bight off the coast of Georgia
-81.121513
-76.343633
31.031829
31.42113
2015-03-16
2017-01-27
North Atlantic Ocean in the South Atlantic Bight off the coast of Georgia; Navy Op Area NA06
0
BCO-DMO catalogue of parameters from Size-fractionated chlorophyll-a data from samples collected during R/V Savannah cruises conducted in the South Atlantic Bight off the coast of Georgia from 2015-2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/816298.rdf
Name: Cruise_ID
Units: unitless
Description: <p>Cruise Identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/816299.rdf
Name: Station_ID
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/816300.rdf
Name: UTC_Date
Units: unitless
Description: <p>Start date for sample collection; Coordinated Universal Time; Format: MM/DD/YYYY</p>
http://lod.bco-dmo.org/id/dataset-parameter/816301.rdf
Name: UTC_Time
Units: unitless
Description: <p>Start time for sample collection; Coordinated Universal Time; Format: hh:mm:ss</p>
http://lod.bco-dmo.org/id/dataset-parameter/816302.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Start date and time of sample collection formatted to ISO8601 standard: YYYY-MM-DDThh:mm:ssZ</p>
http://lod.bco-dmo.org/id/dataset-parameter/816303.rdf
Name: Latitude
Units: decimal degrees North
Description: <p>Start latitude for sample collection; Negative = South</p>
http://lod.bco-dmo.org/id/dataset-parameter/816304.rdf
Name: Longitude
Units: decimal degrees East
Description: <p>Start longitude for sample collection; Negative = West</p>
http://lod.bco-dmo.org/id/dataset-parameter/816305.rdf
Name: Bottom_Depth
Units: meters (m)
Description: <p>Bottom depth from ship's sonar</p>
http://lod.bco-dmo.org/id/dataset-parameter/816306.rdf
Name: Depth
Units: meters (m)
Description: <p>Sample depth from CTD readout</p>
http://lod.bco-dmo.org/id/dataset-parameter/816307.rdf
Name: Total_Chl_a
Units: micrograms per liter (ug/L)
Description: <p>Total chlorophyll-a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/816308.rdf
Name: gt_63_um
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyll-a concentration in plankton greater than 63 micrometers</p>
http://lod.bco-dmo.org/id/dataset-parameter/816309.rdf
Name: bw_63_20_um
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyll-a concentration in plankton in size range 20-63 micrometers</p>
http://lod.bco-dmo.org/id/dataset-parameter/816310.rdf
Name: lt_20_um
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyll-a concentration in plankton smaller than 20 micrometers</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
72303
https://darchive.mblwhoilibrary.org/bitstream/1912/25888/1/dataset-816278_chlorophyll__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.816278.1
download
onLine
dataset
<p>All samples were pre-screened through a 202 µm Nitex mesh. Size-fractionated Chlorophyll a (Chl-a) samples were collected by passing discrete 1 L aliquots of seawater through Nitex with mesh sizes of 63 and 20 µm, and collecting the filtrate. The filtrate was then filtered under low vacuum through GF/F (Sterlitech) filters. An additional sample was directly filtered onto a GF/F filter for total Chl-a. The filters were extracted in 90% acetone overnight at -20°C and analyzed according to the protocol described by Welschmeyer (1994). The size classes were calculated by difference (total, &gt; 63 µm, 63-20 µm, &lt; 20 µm). nd = no data</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- renamed fields;<br />
- added date/time field in ISO8601 format.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Sea-Bird Scientific SBE 911 CTD carousel
Sea-Bird Scientific SBE 911 CTD carousel
PI Supplied Instrument Name: Sea-Bird Scientific SBE 911 CTD carousel Instrument Name: CTD Sea-Bird 911 Instrument Short Name:CTD SBE 911 Instrument Description: The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/
Niskin bottle
Niskin bottle
PI Supplied Instrument Name: Niskin bottle Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
SBE-25 CTD
SBE-25 CTD
PI Supplied Instrument Name: SBE-25 CTD Instrument Name: Sea-Bird SBE 25 Sealogger CTD Instrument Short Name:CTD SBE 25 Instrument Description: The Sea-Bird SBE 25 SEALOGGER CTD is battery powered and is typically used to record data in memory, eliminating the need for a large vessel, electrical sea cable, and on-board computer. All SBE 25s can also operate in real-time, transmitting data via an opto-isolated RS-232 serial port. Temperature and conductivity are measured by the SBE 3F Temperature sensor and SBE 4 Conductivity sensor (same as those used on the premium SBE 9plus CTD). The SBE 25 also includes the SBE 5P (plastic) or 5T (titanium) Submersible Pump and TC Duct. The pump-controlled, TC-ducted flow configuration significantly reduces salinity spiking caused by ship heave, and in calm waters allows slower descent rates for improved resolution of water column features. Pressure is measured by the modular SBE 29 Temperature Compensated Strain-Gauge Pressure sensor (available in eight depth ranges to suit the operating depth requirement). The SBE 25's modular design makes it easy to configure in the field for a wide range of auxiliary sensors, including optional dissolved oxygen (SBE 43), pH (SBE 18 or SBE 27), fluorescence, transmissivity, PAR, and optical backscatter sensors. More information from Sea-Bird Electronics: http:www.seabird.com. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0040/
Turner Designs 10AU Fluorometer
Turner Designs 10AU Fluorometer
PI Supplied Instrument Name: Turner Designs 10AU Fluorometer Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Cruise: SAV-15-04
SAV-15-04
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-04
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-15-16
SAV-15-16
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-16
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-15-20
SAV-15-20
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-20
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-15-26
SAV-15-26
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-26
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-16-06
SAV-16-06
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-06
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-16-22
SAV-16-22
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-22
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-16-28
SAV-16-28
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-28
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-17-02
SAV-17-02
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-02
Peter Lee
College of Charleston - Hollings Marine Lab
R/V Savannah
Community Standard Description
R/V Savannah
vessel