http://lod.bco-dmo.org/id/dataset/816549
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-06-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Porewater nutrient, sulfide, and sulfate concentrations in Alvin pushcore samples collected from Guaymas Basin hydrothermal vents during R/V Atlantis cruise AT42-05 in November 2018 and analyzed at the Max Planck Institute for Marine Microbiology
2020-08-21
publication
2020-08-21
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-24
publication
https://doi.org/10.26008/1912/bco-dmo.816549.1
Andreas Teske
University of North Carolina at Chapel Hill
principalInvestigator
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Wegener, G., Teske, A., Edgcomb, V. (2020) Porewater nutrient, sulfide, and sulfate concentrations in Alvin pushcore samples collected from Guaymas Basin hydrothermal vents during R/V Atlantis cruise AT42-05 in November 2018 and analyzed at the Max Planck Institute for Marine Microbiology. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.816549.1 [access date]
Guaymas nutrients (MPI) Dataset Description: <p>Porewater nutrient, sulfide, and sulfate (NH4+, NO2-, NO3-, PO43-, SiO42-, Cl-, S2-, SO24-) concentrations in Alvin pushcore samples from Guaymas Basin hydrothermal vents, R/V Atlantis cruise AT42-05, Nov. 2018. These samples were analyzed&nbsp;at the Max Planck Institute for Marine Microbiology in Bremen, Germany.</p>
<p>Porewater samples from this cruise were also reported by&nbsp;Woods Hole Oceanographic Institution (NO3+NO2, NH4, PO4) and the University of North Carolina at Chapel Hill (sulfate and sulfide). See related datasets:<br />
<a href="https://www.bco-dmo.org/dataset/773129" target="_blank">https://www.bco-dmo.org/dataset/773129</a>&nbsp;(WHOI)<br />
<a href="https://www.bco-dmo.org/dataset/816852" target="_blank">https://www.bco-dmo.org/dataset/816852</a>&nbsp;(UNC)</p> Methods and Sampling: <p>This porewater dataset was obtained at the Max-Planck-Institute for Marine Microbiology in Bremen, Germany. The sampling site names in the data are based on Teske et al. 2016.</p>
<p>For analyses at the Max-Planck Institute for Marine Microbiology (Gunter Wegener), intact sediment cores were sampled using the Rhizons (Rhizosphere Research Products, Wageningen, NL) as described previously (Seeberg-Elversfeldt et al., 2005). The overlying water was removed from the cores and holes were drilled into designated sediment sampling depths. Pretreated Rhizons were injected and suction was applied with syringes for approx. 30 min. The rhizome depths are given in the dataset (depth_bsf).</p>
<p>For sulfide analysis, 1 ml porewater subsamples were fixed with 0.1 ml of 0.1 M zinc acetate solution to preserve the sulfide as zinc sulfide until analysis by the methylene blue method (Cline 1969). The same fixed porewater sample was used for measuring sulfate concentrations using ion chromatography (Metrohm 930 Compact IC flex oven, Metrosep A PCC HC/4.0 preconcentration column, and Metrosep A Supp 5 Guard/4.0 chromatography column).</p>
<p>The concentrations of ammonia, nitrite, nitrate, phosphate, and silicate were determined from porewater using a continuous flow nutrient analyzer (QuAAtro39; Seal Analytical) as published previously (Grasshoff et al., 2009).</p>
<p>Problem report: Transport problems have caused sample losses among the porewater samples, which are evident in occasional gaps in porewater profiles.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829680 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1829680
completed
Andreas Teske
University of North Carolina at Chapel Hill
508-287-1648
Department of Marine Sciences 3117B Venable Hall, CB 3300
Chapel Hill
NC
27599
USA
teske@email.unc.edu
pointOfContact
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
508-289-3734
Department of Geology and Geophysics 220 McLean Lab, MS #8
Woods Hole
MA
02543
USA
vedgcomb@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Site
Sample_type
Lat
Lon
Water_depth
date
gear
Dive
Core
depth_bsf
Ammonia
Nitrite
Nitrite_Nitrate
Phosphate
Silicate
Nitrate
Chloride
Sulfate
Sulfide
QuAAtro39; Seal Analytical continuous flow nutrient analyzer
Alivn pushcore
ion chromatography
Rhizons
theme
None, User defined
site
sample type
latitude
longitude
depth_w
date
instrument
dive_id
core id
depth below seafloor
Ammonia
Nitrite
Nitrate plus nitrite concentrations in sediment pore water
reactive phosphorus (PO4)
Silicate
Nitrate
chloride
sulfate
sulfide
featureType
BCO-DMO Standard Parameters
Nutrient Autoanalyzer
Alvin tube core
Ion Chromatograph
Sediment Porewater Sampler
instrument
BCO-DMO Standard Instruments
AT42-05
AT42-05_Alvin_Dives
service
Deployment Activity
Guaymas Basin, Gulf of California, Mexico
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Hydrothermal Fungi in the Guaymas Basin Hydrocarbon Ecosystem
https://www.bco-dmo.org/project/770633
Collaborative Research: Hydrothermal Fungi in the Guaymas Basin Hydrocarbon Ecosystem
<p>NSF Award Abstract:<br />
Fungi that can derive energy from chemicals, yet consume other organisms or organic material to obtain carbon have been reported from diverse marine subsurface samples, including from hundreds of meters below the seafloor. Evidence exists that Fungi are active in subsurface marine sediments globally, yet there is a dearth of knowledge on their role in the marine subsurface, and specifically on their role(s) in hydrocarbon degradation within deep-sea sediments. This team is isolating a broad collection of environmentally relevant filamentous Fungi and yeasts from hydrothermally-influenced and hydrocarbon-rich seep sediments of Guaymas Basin using high-throughput culture-based approaches. They aim to reveal the diversity of Fungi and Bacteria in these hydrothermal sediments, how temperature and hydrocarbon composition shape their distribution, and how Fungi cooperate to enhance the degradation of hydrocarbons by Bacteria. By hosting six undergraduates through the WHOI Summer Student Fellows program and the Woods Hole Partnership Education Program, the project contributes to increasing diversity in marine science by offering opportunities for promising undergraduates from disadvantaged populations. High school students are involved in summer projects and in intensive summer workshops. One postdoc, a graduate student, and two Research Associates are supported, and international collaborations are strengthened. The postdoc and graduate student are gaining valuable cruise-based experience. An e-lecture on Fungi and their role(s) in biodegradation of hydrocarbons will be made publicly available by the end of the project. Fungal isolates with accompanying information will be secured in a reference culture collection for long-term storage and are available to any interested researcher throughout the project.</p>
<p>The PIs are isolating a broad collection of environmentally relevant filamentous Fungi and yeasts from hydrothermally-influenced and hydrocarbon-rich seep sediments of Guaymas Basin using high-throughput culture-based approaches, with the aim to reveal their ability to degrade individual hydrocarbons under in situ pressures and temperatures. Culture independent methods marker gene analyses are used to characterize in situ fungal and bacterial diversity and to examine how temperature and hydrocarbon composition shape fungal community composition and distribution. Traditional and comprehensive two-dimensional gas chromatographic analyses are used to examine the complexities and subtle changes in inventories of hydrocarbons within sediment cores, and provide evidence for in situ microbial alteration of individual hydrocarbons. Incubation experiments are used to test the ability of fungal isolates to utilize different hydrocarbons as a sole or auxiliary carbon source under in situ pressures and temperatures and their ability to stimulate biodegradation of hydrocarbons by hydrocarbon-degrading bacteria. Expressed genes within these incubation studies tell us how Fungi and Bacteria couple metabolisms to increase overall specificity and extent of biodegradation of hydrocarbons.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
HOTFUN
largerWorkCitation
project
eng; USA
oceans
Guaymas Basin, Gulf of California, Mexico
-111.4071
-111.4039
27.0069
27.0118
2018-11-17
2018-11-25
Guaymas Basin, Gulf of CA, Mexico
0
BCO-DMO catalogue of parameters from Porewater nutrient, sulfide, and sulfate concentrations in Alvin pushcore samples collected from Guaymas Basin hydrothermal vents during R/V Atlantis cruise AT42-05 in November 2018 and analyzed at the Max Planck Institute for Marine Microbiology
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/816828.rdf
Name: Site
Units: unitless
Description: Site name
http://lod.bco-dmo.org/id/dataset-parameter/816829.rdf
Name: Sample_type
Units: unitless
Description: Description of sample
http://lod.bco-dmo.org/id/dataset-parameter/816830.rdf
Name: Lat
Units: decimal degrees North
Description: Site latitude; positive values = North
http://lod.bco-dmo.org/id/dataset-parameter/816831.rdf
Name: Lon
Units: decimal degrees East
Description: Site longitude; positive values = East
http://lod.bco-dmo.org/id/dataset-parameter/816832.rdf
Name: Water_depth
Units: meters
Description: Depth of the water
http://lod.bco-dmo.org/id/dataset-parameter/816833.rdf
Name: date
Units: unitless
Description: Date of sampling; format: YYYY-MM-DD
http://lod.bco-dmo.org/id/dataset-parameter/816834.rdf
Name: gear
Units: unitless
Description: Sampling gear
http://lod.bco-dmo.org/id/dataset-parameter/816835.rdf
Name: Dive
Units: unitless
Description: Dive ID number
http://lod.bco-dmo.org/id/dataset-parameter/816836.rdf
Name: Core
Units: unitless
Description: Core ID number
http://lod.bco-dmo.org/id/dataset-parameter/816837.rdf
Name: depth_bsf
Units: centimeters (cm)
Description: Depth below sea floor
http://lod.bco-dmo.org/id/dataset-parameter/816838.rdf
Name: Ammonia
Units: micromoles per liter (umol/L)
Description: Ammonia
http://lod.bco-dmo.org/id/dataset-parameter/816839.rdf
Name: Nitrite
Units: micromoles per liter (umol/L)
Description: Nitrite
http://lod.bco-dmo.org/id/dataset-parameter/816840.rdf
Name: Nitrite_Nitrate
Units: micromoles per liter (umol/L)
Description: Nitrite+Nitrate
http://lod.bco-dmo.org/id/dataset-parameter/816841.rdf
Name: Phosphate
Units: micromoles per liter (umol/L)
Description: Phosphate
http://lod.bco-dmo.org/id/dataset-parameter/816842.rdf
Name: Silicate
Units: micromoles per liter (umol/L)
Description: Silicate
http://lod.bco-dmo.org/id/dataset-parameter/816843.rdf
Name: Nitrate
Units: micromoles per liter (umol/L)
Description: Nitrate
http://lod.bco-dmo.org/id/dataset-parameter/816844.rdf
Name: Chloride
Units: millimolar (mM)
Description: Chloride
http://lod.bco-dmo.org/id/dataset-parameter/816846.rdf
Name: Sulfate
Units: millimolar (mM)
Description: Sulfate
http://lod.bco-dmo.org/id/dataset-parameter/816849.rdf
Name: Sulfide
Units: millimolar (mM)
Description: Sulfide
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
12797
https://darchive.mblwhoilibrary.org/bitstream/1912/26113/1/dataset-816549_hydrothermal-vent-porewater-nutrients-mpi__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.816549.1
download
onLine
dataset
<p>This porewater dataset was obtained at the Max-Planck-Institute for Marine Microbiology in Bremen, Germany. The sampling site names in the data are based on Teske et al. 2016.</p>
<p>For analyses at the Max-Planck Institute for Marine Microbiology (Gunter Wegener), intact sediment cores were sampled using the Rhizons (Rhizosphere Research Products, Wageningen, NL) as described previously (Seeberg-Elversfeldt et al., 2005). The overlying water was removed from the cores and holes were drilled into designated sediment sampling depths. Pretreated Rhizons were injected and suction was applied with syringes for approx. 30 min. The rhizome depths are given in the dataset (depth_bsf).</p>
<p>For sulfide analysis, 1 ml porewater subsamples were fixed with 0.1 ml of 0.1 M zinc acetate solution to preserve the sulfide as zinc sulfide until analysis by the methylene blue method (Cline 1969). The same fixed porewater sample was used for measuring sulfate concentrations using ion chromatography (Metrohm 930 Compact IC flex oven, Metrosep A PCC HC/4.0 preconcentration column, and Metrosep A Supp 5 Guard/4.0 chromatography column).</p>
<p>The concentrations of ammonia, nitrite, nitrate, phosphate, and silicate were determined from porewater using a continuous flow nutrient analyzer (QuAAtro39; Seal Analytical) as published previously (Grasshoff et al., 2009).</p>
<p>Problem report: Transport problems have caused sample losses among the porewater samples, which are evident in occasional gaps in porewater profiles.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
&nbsp;- renamed fields;<br />
&nbsp;- removed empty columns;<br />
&nbsp;- changed date format from DD.MM.YYYY to YYYY-MM-DD;<br />
&nbsp;- added Site, Sample_type, Lat, Lon, and Water_depth fields from information originally provided in Word;<br />
&nbsp;- removed commas in Site and&nbsp;Sample_type columns;<br />
- joined data columns from separate spreadsheets into one data by matching on key Dive+Core+depth_bsf.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
QuAAtro39; Seal Analytical continuous flow nutrient analyzer
QuAAtro39; Seal Analytical continuous flow nutrient analyzer
PI Supplied Instrument Name: QuAAtro39; Seal Analytical continuous flow nutrient analyzer Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Alivn pushcore
Alivn pushcore
PI Supplied Instrument Name: Alivn pushcore Instrument Name: Alvin tube core Instrument Short Name:Tube Core Instrument Description: A plastic tube, about 40 cm (16 inches) long, is pushed into the sediment by Alvin's manipulator arm to collect a sediment core.
ion chromatography
ion chromatography
PI Supplied Instrument Name: ion chromatography PI Supplied Instrument Description:Sulfate concentrations were measured using ion chromatography (Metrohm 930 Compact IC flex oven, Metrosep A PCC HC/4.0 preconcentration column, and Metrosep A Supp 5 Guard/4.0 chromatography column). Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)
Rhizons
Rhizons
PI Supplied Instrument Name: Rhizons Instrument Name: Sediment Porewater Sampler Instrument Short Name: Instrument Description: A device that collects samples of pore water from various horizons below the seabed.
Cruise: AT42-05
AT42-05
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT42-05
Andreas Teske
University of North Carolina at Chapel Hill
Deployment: AT42-05_Alvin_Dives
AT42-05_Alvin_Dives
Alvin
Community Standard Description
International Council for the Exploration of the Sea
Alvin
submarine-manned
AT42-05_Alvin_Dives
Andreas Teske
University of North Carolina at Chapel Hill
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
Alvin
Community Standard Description
International Council for the Exploration of the Sea
Alvin
submarine-manned