http://lod.bco-dmo.org/id/dataset/816852
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-06-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Porewater sulfate and sulfide concentrations in Alvin pushcore samples collected from Guaymas Basin hydrothermal vents during R/V Atlantis cruise AT42-05 in November 2018 and analyzed at UNC Chapel Hill
2020-06-26
publication
2020-06-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-21
publication
https://doi.org/10.26008/1912/bco-dmo.816852.1
Andreas Teske
University of North Carolina at Chapel Hill
principalInvestigator
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Chambers, C., Teske, A., Edgcomb, V. (2020) Porewater sulfate and sulfide concentrations in Alvin pushcore samples collected from Guaymas Basin hydrothermal vents during R/V Atlantis cruise AT42-05 in November 2018 and analyzed at UNC Chapel Hill. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-06-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.816852.1 [access date]
Guaymas sulfate & sulfide (UNC Chapel Hill) Dataset Description: <p>Porewater sulfate and sulfide concentrations in Alvin pushcore samples collected from Guaymas Basin hydrothermal vents during R/V Atlantis cruise AT42-05 in November 2018. This dataset was obtained at the University of North Carolina Chapel Hill.</p>
<p>Porewater sulfate and sulfide samples from this cruise were also analyzed at the Max Planck Institute for Marine Microbiology. See related dataset: <a href="https://www.bco-dmo.org/dataset/816549" target="_blank">https://www.bco-dmo.org/dataset/816549</a> (MPI)</p> Methods and Sampling: <p>This porewater dataset was obtained by Chris Chambers at the University of North Carolina Chapel Hill. The sampling site names in the data are based on Teske et al. 2016.</p>
<p>For analysis at UNC (Chris Chambers), porewater was obtained from freshly collected sediment push cores on R/V Atlantis. The sediment cores were sampled in 3 cm intervals; ca. 40 ml sediment samples were centrifuged in 50 ml conical Falcon tubes for 5 to 10 minutes at approx. 1000 g, until the sediment had settled and produced ca. 8 to 10 ml of porewater. The sediment interval depths are given in the dataset (Sediment_depth). For porewater sulfide analysis, 1 ml porewater subsamples were drawn into syringes, filtered immediately through 0.45 μm filters, and placed in Eppendorf sample vials each containing 0.1 ml of 0.1 M zinc acetate solution to preserve the sulfide as zinc sulfide until analyzed. Sulfide was quantified spectrophotometrically at UNC-Chapel Hill using the methylene blue method (Cline 1969). For sulfate analysis, 1 ml porewater samples were acidified with 50 microliters of 1 N HCl, and bubbled with N2 for 1 minute to remove hydrogen sulfide. After returning the samples to the home laboratory, sulfate concentrations were determined using the ion chromatograph of the UNC Environmental Program.</p>
<p>Problem report: Transport problems have caused sample losses among the porewater samples, which are evident in occasional gaps in porewater profiles.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829680 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829680
onGoing
Andreas Teske
University of North Carolina at Chapel Hill
508-287-1648
Department of Marine Sciences 3117B Venable Hall, CB 3300
Chapel Hill
NC
27599
USA
teske@email.unc.edu
pointOfContact
Virginia P. Edgcomb
Woods Hole Oceanographic Institution
508-289-3734
Department of Geology and Geophysics 220 McLean Lab, MS #8
Woods Hole
MA
02543
USA
vedgcomb@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Site
Sample_type
Lat
Lon
Water_depth
Date
Dive
Core
Sediment_depth
Sulfate
Sulfide
Alivn pushcore
theme
None, User defined
site
sample type
latitude
longitude
depth_w
date
dive_id
core id
depth core
sulfate
sulfide
featureType
BCO-DMO Standard Parameters
Alvin tube core
Ion Chromatograph
Spectrophotometer
instrument
BCO-DMO Standard Instruments
AT42-05
AT42-05_Alvin_Dives
service
Deployment Activity
Guaymas Basin, Gulf of California, Mexico
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Hydrothermal Fungi in the Guaymas Basin Hydrocarbon Ecosystem
https://osprey.bco-dmo.org/project/770633
Collaborative Research: Hydrothermal Fungi in the Guaymas Basin Hydrocarbon Ecosystem
<p>NSF Award Abstract:<br />
Fungi that can derive energy from chemicals, yet consume other organisms or organic material to obtain carbon have been reported from diverse marine subsurface samples, including from hundreds of meters below the seafloor. Evidence exists that Fungi are active in subsurface marine sediments globally, yet there is a dearth of knowledge on their role in the marine subsurface, and specifically on their role(s) in hydrocarbon degradation within deep-sea sediments. This team is isolating a broad collection of environmentally relevant filamentous Fungi and yeasts from hydrothermally-influenced and hydrocarbon-rich seep sediments of Guaymas Basin using high-throughput culture-based approaches. They aim to reveal the diversity of Fungi and Bacteria in these hydrothermal sediments, how temperature and hydrocarbon composition shape their distribution, and how Fungi cooperate to enhance the degradation of hydrocarbons by Bacteria. By hosting six undergraduates through the WHOI Summer Student Fellows program and the Woods Hole Partnership Education Program, the project contributes to increasing diversity in marine science by offering opportunities for promising undergraduates from disadvantaged populations. High school students are involved in summer projects and in intensive summer workshops. One postdoc, a graduate student, and two Research Associates are supported, and international collaborations are strengthened. The postdoc and graduate student are gaining valuable cruise-based experience. An e-lecture on Fungi and their role(s) in biodegradation of hydrocarbons will be made publicly available by the end of the project. Fungal isolates with accompanying information will be secured in a reference culture collection for long-term storage and are available to any interested researcher throughout the project.</p>
<p>The PIs are isolating a broad collection of environmentally relevant filamentous Fungi and yeasts from hydrothermally-influenced and hydrocarbon-rich seep sediments of Guaymas Basin using high-throughput culture-based approaches, with the aim to reveal their ability to degrade individual hydrocarbons under in situ pressures and temperatures. Culture independent methods marker gene analyses are used to characterize in situ fungal and bacterial diversity and to examine how temperature and hydrocarbon composition shape fungal community composition and distribution. Traditional and comprehensive two-dimensional gas chromatographic analyses are used to examine the complexities and subtle changes in inventories of hydrocarbons within sediment cores, and provide evidence for in situ microbial alteration of individual hydrocarbons. Incubation experiments are used to test the ability of fungal isolates to utilize different hydrocarbons as a sole or auxiliary carbon source under in situ pressures and temperatures and their ability to stimulate biodegradation of hydrocarbons by hydrocarbon-degrading bacteria. Expressed genes within these incubation studies tell us how Fungi and Bacteria couple metabolisms to increase overall specificity and extent of biodegradation of hydrocarbons.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
HOTFUN
largerWorkCitation
project
eng; USA
oceans
Guaymas Basin, Gulf of California, Mexico
-111.4073
-111.4039
27.0011
27.0118
2018-11-17
2018-11-26
Guaymas Basin, Gulf of CA, Mexico
0
BCO-DMO catalogue of parameters from Porewater sulfate and sulfide concentrations in Alvin pushcore samples collected from Guaymas Basin hydrothermal vents during R/V Atlantis cruise AT42-05 in November 2018 and analyzed at UNC Chapel Hill
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/816874.rdf
Name: Site
Units: unitless
Description: <p>Site name</p>
http://lod.bco-dmo.org/id/dataset-parameter/816875.rdf
Name: Sample_type
Units: unitless
Description: <p>Description of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/816876.rdf
Name: Lat
Units: decimal degrees North
Description: <p>Site latitude; positive values = North</p>
http://lod.bco-dmo.org/id/dataset-parameter/816877.rdf
Name: Lon
Units: decimal degrees East
Description: <p>Site longitude; positive values = East</p>
http://lod.bco-dmo.org/id/dataset-parameter/816878.rdf
Name: Water_depth
Units: meters
Description: <p>Depth of the water</p>
http://lod.bco-dmo.org/id/dataset-parameter/816879.rdf
Name: Date
Units: unitless
Description: <p>Date of sampling; format: YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/816880.rdf
Name: Dive
Units: unitless
Description: <p>Dive ID number</p>
http://lod.bco-dmo.org/id/dataset-parameter/816881.rdf
Name: Core
Units: unitless
Description: <p>Core ID number</p>
http://lod.bco-dmo.org/id/dataset-parameter/816882.rdf
Name: Sediment_depth
Units: centimeters
Description: <p>Sample depth within the core</p>
http://lod.bco-dmo.org/id/dataset-parameter/816883.rdf
Name: Sulfate
Units: millimolar (mM)
Description: <p>Sulfate</p>
http://lod.bco-dmo.org/id/dataset-parameter/816884.rdf
Name: Sulfide
Units: millimolar (mM)
Description: <p>Sulfide</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7096
https://darchive.mblwhoilibrary.org/bitstream/1912/26104/1/dataset-816852_hydrothermal-vent-porewater-sulfate-and-sulfide-unc__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.816852.1
download
onLine
dataset
<p>This porewater dataset was obtained by Chris Chambers at the University of North Carolina Chapel Hill. The sampling site names in the data are based on Teske et al. 2016.</p>
<p>For analysis at UNC (Chris Chambers), porewater was obtained from freshly collected sediment push cores on R/V Atlantis. The sediment cores were sampled in 3 cm intervals; ca. 40 ml sediment samples were centrifuged in 50 ml conical Falcon tubes for 5 to 10 minutes at approx. 1000 g, until the sediment had settled and produced ca. 8 to 10 ml of porewater. The sediment interval depths are given in the dataset (Sediment_depth). For porewater sulfide analysis, 1 ml porewater subsamples were drawn into syringes, filtered immediately through 0.45 μm filters, and placed in Eppendorf sample vials each containing 0.1 ml of 0.1 M zinc acetate solution to preserve the sulfide as zinc sulfide until analyzed. Sulfide was quantified spectrophotometrically at UNC-Chapel Hill using the methylene blue method (Cline 1969). For sulfate analysis, 1 ml porewater samples were acidified with 50 microliters of 1 N HCl, and bubbled with N2 for 1 minute to remove hydrogen sulfide. After returning the samples to the home laboratory, sulfate concentrations were determined using the ion chromatograph of the UNC Environmental Program.</p>
<p>Problem report: Transport problems have caused sample losses among the porewater samples, which are evident in occasional gaps in porewater profiles.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
&nbsp;- added columns for Site, Sample_type, Lat, Lon, Date, Dive, Core, and Water_depth fields from header rows;<br />
&nbsp;- renamed fields;<br />
&nbsp;- removed commas in Site and&nbsp;Sample_type columns.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Alivn pushcore
Alivn pushcore
PI Supplied Instrument Name: Alivn pushcore Instrument Name: Alvin tube core Instrument Short Name:Tube Core Instrument Description: A plastic tube, about 40 cm (16 inches) long, is pushed into the sediment by Alvin's manipulator arm to collect a sediment core.
PI Supplied Instrument Name: PI Supplied Instrument Description:Sulfate concentrations were determined using the ion chromatograph of the UNC Environmental Program. Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)
PI Supplied Instrument Name: PI Supplied Instrument Description:Sulfide was quantified spectrophotometrically at UNC-Chapel Hill. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: AT42-05
AT42-05
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT42-05
Andreas Teske
University of North Carolina at Chapel Hill
Deployment: AT42-05_Alvin_Dives
AT42-05_Alvin_Dives
HOV Alvin
Community Standard Description
International Council for the Exploration of the Sea
HOV Alvin
submarine-manned
AT42-05_Alvin_Dives
Andreas Teske
University of North Carolina at Chapel Hill
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
HOV Alvin
Community Standard Description
International Council for the Exploration of the Sea
HOV Alvin
submarine-manned