http://lod.bco-dmo.org/id/dataset/817454 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2020-07-01 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Unprocessed holographic data of cryopeg fluids viewed at sub-micron resolution from Alaskan Arctic Coast Permafrost Tunnel and landfast sea ice from May 2017 2020-07-01 publication 2020-07-01 revision Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA) 2020-07-16 publication https://doi.org/10.26008/1912/bco-dmo.817454.1 Jody W. Deming University of Washington principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Deming, J. (2020) Unprocessed holographic data of cryopeg fluids viewed at sub-micron resolution from Alaskan Arctic Coast Permafrost Tunnel and landfast sea ice from May 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-07-01 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.817454.1 [access date] Unprocessed holographic data of cryopeg fluids viewed at sub-micron resolution Dataset Description: &lt;p&gt;Brine samples were collected from both sea ice and cryopeg near Utqiaġvik, Alaska, USA. Snow and ice thickness along with sackhole core depth information are available for sea ice samples. Bacterial and viral abundances along with temperature, pH, salinity, inorganic nutrients, organic nutrients, EPS, and water isotopes were measured for select samples.&lt;/p&gt; Methods and Sampling: &lt;div&gt;Sackhole and cryopeg brines were collected according to Cooper et al., 2019 FEMS Environmental Microbiology and inserted into the holographic microscope using a syringe as described in Lindensmith et al., 2016 PLOS One.&lt;/div&gt; &lt;div&gt;&amp;nbsp;&lt;/div&gt; &lt;div&gt;Experiments were completed at the sample site or in the field-laboratory using a chemotactic chamber as described in Lindensmith, et al., 2016 PLOS One. Chemical gradients of serine (prepared to 1 molar concentration in self-same salinity solution) or gradients of salinity (established from sample salinity to seawater) were used to stimulate taxis across the chamber, or the a heating block was used to create temperature gradients in a cold room. Motility was observed in the microscope sub-sampling over periods of seconds or hours, according to timestamp data (available in the hologram folder).&lt;/div&gt; Funding provided by Gordon and Betty Moore Foundation: Marine Microbiology Initiative (MMI) Award Number: GBMF5488 Award URL: https://www.moore.org/grant-detail?grantId=GBMF5488 completed Jody W. Deming University of Washington 206-543-0845 School of Oceanography Box 357640, University of Washington Seattle WA 98195 USA jdeming@uw.edu pointOfContact asNeeded Dataset Version: 1 Unknown holographic microscope theme None, User defined Digital inline holographic microscope instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Marine Microbiology Initiative https://www.moore.org/initiative-strategy-detail?initiativeId=marine-microbiology-initiative Marine Microbiology Initiative A Gordon and Betty Moore Foundation Program. Forging a new paradigm in marine microbial ecology: Microbes in the ocean produce half of the oxygen on the planet and remove vast amounts of carbon dioxide, a greenhouse gas, from the atmosphere. Yet, we have known surprisingly little about these microscopic organisms. As we discover answers to some long-standing puzzles about the roles that marine microorganisms play in supporting the ocean’s food webs and driving global elemental cycles, we realized that we still need to learn much more about what these organisms do and how they do it—including how they evolved and contribute to our ocean's health and productivity. The Marine Microbiology Initiative seeks to gain a comprehensive understanding of marine microbial communities, including their diversity, functions and behaviors; their ecological roles; and their origins and evolution. Our focus has been to enable researchers to uncover the principles that govern the interactions among microbes and that govern microbially mediated nutrient flow in the sea. To address these opportunities, we support leaders in the field through investigator awards, multidisciplinary team research projects, and efforts to create resources of broad use to the research community. We also support development of new instrumentation, tools, technologies and genetic approaches. Through the efforts of many scientists from around the world, the initiative has been catalyzing new science through advances in methods and technology, and to reduce interdisciplinary barriers slowing progress. With our support, researchers are quantifying nutrient pools in the ocean, deciphering the genetic and biochemical bases of microbial metabolism, and understanding how microbes interact with one another. The initiative has five grant portfolios: Individual investigator awards for current and emerging leaders in the field. Multidisciplinary projects that support collaboration across disciplines. New instrumentation, tools and technology that enable scientists to ask new questions in ways previously not possible. Community resource efforts that fund the creation and sharing of data and the development of tools, methods and infrastructure of widespread utility. Projects that advance genetic tools to enable development of experimental model systems in marine microbial ecology. We also bring together scientists to discuss timely subjects and to facilitate scientific exchange. Our path to marine microbial ecology was a confluence of new technology that could accelerate science and an opportunity to support a field that was not well funded relative to potential impact. Around the time we began this work in 2004, the life sciences were entering a new era of DNA sequencing and genomics, expanding possibilities for scientific research – including the nascent field of marine microbial ecology. Through conversations with pioneers inside and outside the field, an opportunity was identified: to apply these new sequencing tools to advance knowledge of marine microbial communities and reveal how they support and influence ocean systems. After many years of success, we will wind down this effort and close the initiative in 2021. We will have invested more than $250 million over 17 years to deepen understanding of the diversity, ecological activities and evolution of marine microbial communities. Thanks to the work of hundreds of scientists and others involved with the initiative, the goals have been achieved and the field has been profoundly enriched; it is now positioned to address new scientific questions using innovative technologies and methods. MMI largerWorkCitation program Understanding How Virus Infection Affects Gene Flow and Microbial Evolution in Extreme Polar Environments https://osprey.bco-dmo.org/project/789280 Understanding How Virus Infection Affects Gene Flow and Microbial Evolution in Extreme Polar Environments <p>GBMF Summary:</p> <p>In support of developing a virus–bacterium–alga culture system and advancing methods to investigate how virus infection and stress impact gene flow and microbial evolution in cold, highly saline environments.</p> Arctic Subzero Brines largerWorkCitation project eng; USA oceans -156.7294 -156.5049 71.2944 71.473 2017-05-06 2017-05-10 0 BCO-DMO catalogue of parameters from Unprocessed holographic data of cryopeg fluids viewed at sub-micron resolution from Alaskan Arctic Coast Permafrost Tunnel and landfast sea ice from May 2017 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 32023865247 https://darchive.mblwhoilibrary.org/bitstream/1912/25977/1/images.tar.gz download https://doi.org/10.26008/1912/bco-dmo.817454.1 download onLine dataset &lt;div&gt;Sackhole and cryopeg brines were collected according to Cooper et al., 2019 FEMS Environmental Microbiology and inserted into the holographic microscope using a syringe as described in Lindensmith et al., 2016 PLOS One.&lt;/div&gt; &lt;div&gt;&amp;nbsp;&lt;/div&gt; &lt;div&gt;Experiments were completed at the sample site or in the field-laboratory using a chemotactic chamber as described in Lindensmith, et al., 2016 PLOS One. Chemical gradients of serine (prepared to 1 molar concentration in self-same salinity solution) or gradients of salinity (established from sample salinity to seawater) were used to stimulate taxis across the chamber, or the a heating block was used to create temperature gradients in a cold room. Motility was observed in the microscope sub-sampling over periods of seconds or hours, according to timestamp data (available in the hologram folder).&lt;/div&gt; Specified by the Principal Investigator(s) &lt;p&gt;BCO-DMO Processing Notes:&lt;br /&gt; - packaged all images into tar gzipped file.&lt;/p&gt; &lt;p&gt;&amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact holographic microscope holographic microscope PI Supplied Instrument Name: holographic microscope PI Supplied Instrument Description:Sackhole and cryopeg brines were collected according to Cooper et al., 2019 FEMS Environmental Microbiology and inserted into the holographic microscope using a syringe as described in Lindensmith et al., 2016 PLOS One. Instrument Name: Digital inline holographic microscope Instrument Short Name:DIHM Instrument Description: A Digital Inline Holographic Microscope (DIHM) uses coherent (laser) light and a digital camera to image objects with micrometer scale resolution. A portion of the light scattered by illuminated objects interferes with incident light in a predictable manner. The resulting interference patterns projected onto a two-dimensional plane (i.e. digital camera sensor) are recorded as holograms. These digital holograms are then numerically reconstructed to produce an in-focus image at a given distance from the recording plane. A relatively large illuminated volume (>100 mL) can be reconstructed in this manner to produce a single image with an extended depth of field.