<div><p>Methodology:</p>
<p>Ca. T. autotrophicus strain EF1 protein extracts were analyzed using a mass spectrometer. Protein identifications were determined using COMET and checked using PeptideProphet, ProteinProphet, and iProphet. The final list of protein identifications were then filtered to ensure that at least one peptide was observed consistently across individual injections and that the protein probability within each replicate was greater than 95%. A total of 802 proteins were observed, representing 53% of all predicted proteins in strain EF1.</p>
<p>Sampling and analytical procedures:</p>
<p>Protein samples for comparative analyses were extracted from axenic cultures grown under aerobic and anaerobic conditions.</p>
<p>Instruments:<br />
Waters ACQUITY M-class UPLC coupled to a Thermo QExactive HF Orbitrap high-resolution mass spectrometer (HRMS) equipped with a nano-electrospray (NSI) source.</p></div>
<div><p>The mass spectrometry proteomics data files have been deposited to the ProteomeXchange Consortium (<a href="http://proteomecentral.proteomexchange.org">http://proteomecentral.proteomexchange.org</a>) via the PRIDE partner repository with the data set identifier PXD013243 and title "Morphological plasticity in a sulfur-oxidizing bacterium from the SUP05 clade enhances dark carbon fixation"<br />
(see <a href="https://www.ebi.ac.uk/pride/archive/projects/PXD013243">https://www.ebi.ac.uk/pride/archive/projects/PXD013243</a>).</p>
<p>These data were published in Shah et al. (2019).</p></div>
Mass spectrometry proteomics data
819147
Mass spectrometry proteomics data
2020-07-21T15:59:54-04:00
2020-07-21T15:59:54-04:00
2020-07-21T16:24:40-04:00
urn:bcodmo:dataset:819147
Mass spectrometry proteomics data investigating morphological plasticity in a sulfur-oxidizing bacterium from the SUP05 clade enhances dark carbon fixation from cultures grown under under aerobic and anaerobic conditions
false
Morris, R., Ingalls, A. E. (2019) Mass spectrometry proteomics data investigating morphological plasticity in a sulfur-oxidizing bacterium from the SUP05 clade enhances dark carbon fixation from cultures grown under under aerobic and anaerobic conditions. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-12-17 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/819147 [access date]
false
1
false
2019-12-17
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