http://lod.bco-dmo.org/id/dataset/819601
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-07-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Dissolved Organic Carbon concentrations collected at the Mo’orea LTER forereef and back reef sites during the 2019 coral bleaching event.
2021-07-09
publication
2021-07-09
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-07-09
publication
https://doi.org/10.26008/1912/bco-dmo.819601.1
Andrew Thurber
Oregon State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Thurber, A. (2021) Dissolved Organic Carbon concentrations collected at the Mo’orea LTER forereef and back reef sites during the 2019 coral bleaching event. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-09 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.819601.1 [access date]
Methods and Sampling: <p>Samples were collected either via Niskin bottle or by hand on SCUBA at Mo’orea, French Polynesia during the 2019 coral bleaching event (April, May).&nbsp; Time is approximate (within + or - 2 hours) because the time reported is the dive time, not the exact time of sample collection.&nbsp;&nbsp;<br />
<br />
In all cases new Whirl Pak bags were used to collect the water samples, which were&nbsp;immediately put on ice and transported back to the lab.&nbsp;The water samples were filtered through precombusted Whatman GF/Fs and the water that passed through was collected in an acid washed container, and then frozen.&nbsp; All filtration equipment was acid washed and Milli-Q washed, and then&nbsp;flushed with sample water prior to sample filtration and water collection.</p>
<p>Samples were kept frozen at -20&nbsp;degrees&nbsp;C until analysis.&nbsp; All analyses were completed within&nbsp;6 months of collection by&nbsp;Nutrient Analytical Services at the Chesapeake Biological Laboratory of the University of Maryland Center for Environmental Science.</p>
<p>Samples were acidified and sparged with ultra-pure carrier-grade air to drive off inorganic carbon and analyzed with a high-temperature combustion (680 C) method on the Shimadzu TOC-L.&nbsp; Operating procedures followed those outlined for 'Total and Dissolved Organic Carbon Method' (https://www.umces.edu/nasl/methods). In short, all carbon compounds are broken down into CO2 which is carried by ultra-pure air to a non-dispersive infrared detector (NDIR) for detection. The system was calibrated before and after each run with potassium hydrogen phthalate standards (6-point curve, 0-20 mg C/L) and referenced against a certified reference control sample (Scp Science Accuspec Toc Standard), a spike (20 ppm) and a blank every 10 samples. The acceptance criteria used was 0.995.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1933165 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1933165
completed
Andrew Thurber
Oregon State University
541-737-4500
athurber@ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
ISO_DateTime_UTC
Latitude
Longitude
Unique_Sample_Identifier
Site_Name
Water_Depth
Reef_Type
DOC
Date
Local_Time
Niskin bottle
SCUBA
Shimadzu TOC-L
theme
None, User defined
ISO_DateTime_UTC
latitude
longitude
sample identification
site
depth
site description
dissolved organic Carbon
date
time of day
featureType
BCO-DMO Standard Parameters
Niskin bottle
Self-Contained Underwater Breathing Apparatus
Shimadzu TOC-L Analyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
RAPID: Ecosystem impact of a coral bleaching event: The role of coral exudates in shifting oligotrophic biogeochemistry and reef microbiomes.
https://osprey.bco-dmo.org/project/776864
RAPID: Ecosystem impact of a coral bleaching event: The role of coral exudates in shifting oligotrophic biogeochemistry and reef microbiomes.
<p>NSF Award Abstract:<br />
Shallow tropical reefs are biodiversity hotspots. Their ecosystem services make them key areas of economic, ecological, and cultural importance. Yet coral reefs are under significant threat due to both local and global stressors which can lead to coral bleaching, disease, and eventually coral death. When corals bleach, they release materials such as dead tissue, mucus, bacteria, and viruses that may affect the entire ecosystem. This project uses a wide-spread bleaching event at a Long Term Ecological Research site in French Polynesia to explore how these released materials impact the reef ecosystem The water chemistry and microbes associated with the corals and surrounding water are examined. This research aims to better understand how corals interact with their environment and how this interaction changes when corals are stressed. Throughout this project two female graduate students are being trained and interactive programs are used to communicate results to elementary, high school, and undergraduate students in Oregon. A 20-minute documentary for web release focusing on the reef scale impact of coral bleaching is in preparation together with film students.</p>
<p>Coral exudates include particulate and dissolved material (sloughed tissue, mucus, bacteria, viruses) that together add limiting nutrients and carbon compounds to the reef, fueling auto- and heterotrophic bacterial production. In recent experiments, coral-bleaching derived exudates were observed to themselves cause rapid bleaching, and often mortality, of previously healthy corals. Importantly, these negative impacts of coral exudate exposure were far greater than thermal stress. These experiments provided insight into a novel mechanism in which bleaching corals can adversely affect the health of adjacent corals. This project leverages these data and an extensive and ongoing bleaching event on the island of Mo?orea to quantify the cascading effects of coral exudates on reef ecosystems. It is hypothesized that during widespread bleaching: (1) DOC is significantly elevated across the reefscape; (2) coral holobiont components become enriched in the pelagic microbiome; (3) the water column microbial community shifts in function to increased heterotrophy and pathogenicity; and (4) coral holobionts diverge from their previous stable state leading to coral reef dysbiosis and/or disease and mortality. Sampling throughout the course of an ongoing bleaching event in Mo?orea is used to quantify the effects of this bleaching event on DOM dynamics and reef health. Mo'orea is situated in the MCR Long Term Ecological Research (MCR LTER) site. The rare reef-scale bleaching event at this well-studied location provides the unusual opportunity to quantify the impact of coral exudates on reef health and to better understand the temporal and spatial impacts of an island-wide bleaching event in an oligotrophic ecosystem. Measurement of the amount of organic matter released per unit area of coral on an island where the reef ecosystem is well parameterized over time and space allows development of a model of the impact of bleaching events on the island carbon budget.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Ecosystem Impacts of Coral Bleaching
largerWorkCitation
project
eng; USA
oceans
-149.8496067
-149.8023833
-17.48237222
-17.46983333
2019-04-30
2019-05-10
Mo’orea, French Polynesia
0
BCO-DMO catalogue of parameters from Dissolved Organic Carbon concentrations collected at the Mo’orea LTER forereef and back reef sites during the 2019 coral bleaching event.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/855420.rdf
Name: ISO_DateTime_UTC
Units: units
Description: <p>Collection and/or dive time in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/855421.rdf
Name: Latitude
Units: units
Description: <p>Latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/855422.rdf
Name: Longitude
Units: units
Description: <p>Longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/855423.rdf
Name: Unique_Sample_Identifier
Units: units
Description: <p>Unique sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/855424.rdf
Name: Site_Name
Units: unitless
Description: <p>Refers to the Mo'orea Long Term Ecological Research site where the sample was collected. Offshore sites are due north of the LTER sites.</p>
http://lod.bco-dmo.org/id/dataset-parameter/855425.rdf
Name: Water_Depth
Units: units
Description: <p>Depth at which the water sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/855426.rdf
Name: Reef_Type
Units: units
Description: <p>Type of reef over which the water sample was collected. Back_Reef = island side of the reef crest, Forereef = ocean side of the reef crest, Offshore = away from the reef</p>
http://lod.bco-dmo.org/id/dataset-parameter/855427.rdf
Name: DOC
Units: units
Description: <p>Dissolved Organic Carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/855428.rdf
Name: Date
Units: units
Description: <p>Date of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/855429.rdf
Name: Local_Time
Units: units
Description: <p>Local time of sample collection and/or dive time</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6765
https://darchive.mblwhoilibrary.org/bitstream/1912/27323/1/dataset-819601_dom-during-coral-bleaching__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.819601.1
download
onLine
dataset
<p>Samples were collected either via Niskin bottle or by hand on SCUBA at Mo’orea, French Polynesia during the 2019 coral bleaching event (April, May).&nbsp; Time is approximate (within + or - 2 hours) because the time reported is the dive time, not the exact time of sample collection.&nbsp;&nbsp;<br />
<br />
In all cases new Whirl Pak bags were used to collect the water samples, which were&nbsp;immediately put on ice and transported back to the lab.&nbsp;The water samples were filtered through precombusted Whatman GF/Fs and the water that passed through was collected in an acid washed container, and then frozen.&nbsp; All filtration equipment was acid washed and Milli-Q washed, and then&nbsp;flushed with sample water prior to sample filtration and water collection.</p>
<p>Samples were kept frozen at -20&nbsp;degrees&nbsp;C until analysis.&nbsp; All analyses were completed within&nbsp;6 months of collection by&nbsp;Nutrient Analytical Services at the Chesapeake Biological Laboratory of the University of Maryland Center for Environmental Science.</p>
<p>Samples were acidified and sparged with ultra-pure carrier-grade air to drive off inorganic carbon and analyzed with a high-temperature combustion (680 C) method on the Shimadzu TOC-L.&nbsp; Operating procedures followed those outlined for 'Total and Dissolved Organic Carbon Method' (https://www.umces.edu/nasl/methods). In short, all carbon compounds are broken down into CO2 which is carried by ultra-pure air to a non-dispersive infrared detector (NDIR) for detection. The system was calibrated before and after each run with potassium hydrogen phthalate standards (6-point curve, 0-20 mg C/L) and referenced against a certified reference control sample (Scp Science Accuspec Toc Standard), a spike (20 ppm) and a blank every 10 samples. The acceptance criteria used was 0.995.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO processing notes:<br />
-&nbsp;Renamed column headers to comply with database requirements<br />
-&nbsp;Rounded latitude and longitude fields to 7 decimal places<br />
- Combined date and local time fields, then&nbsp;converted to ISO 8601 format (UTC)</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Niskin bottle
Niskin bottle
PI Supplied Instrument Name: Niskin bottle PI Supplied Instrument Description:Samples were collected either via Niskin bottle or by hand on SCUBA at Mo’orea, French Polynesia Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
SCUBA
SCUBA
PI Supplied Instrument Name: SCUBA PI Supplied Instrument Description:Samples were collected either via Niskin bottle or by hand on SCUBA at Mo’orea, French Polynesia Instrument Name: Self-Contained Underwater Breathing Apparatus Instrument Short Name:SCUBA Instrument Description: The self-contained underwater breathing apparatus or scuba diving system is the result of technological developments and innovations that began almost 300 years ago. Scuba diving is the most extensively used system for breathing underwater by recreational divers throughout the world and in various forms is also widely used to perform underwater work for military, scientific, and commercial purposes.
Reference: https://oceanexplorer.noaa.gov/technology/technical/technical.html
Shimadzu TOC-L
Shimadzu TOC-L
PI Supplied Instrument Name: Shimadzu TOC-L PI Supplied Instrument Description:A high-temperature combustion (680C) method on the Shimadzu TOC-L following operating procedures outlined in: Total and Dissolved Organic Carbon Method: https://www.umces.edu/nasl/methods was used to analyse the samples. Instrument Name: Shimadzu TOC-L Analyzer Instrument Short Name:Shimadzu TOC-L Instrument Description: A Shimadzu TOC-L Analyzer measures DOC by high temperature combustion method.
Developed by Shimadzu, the 680 degree C combustion catalytic oxidation method is now used worldwide. One of its most important features is the capacity to efficiently oxidize hard-to-decompose organic compounds, including insoluble and macromolecular organic compounds. The 680 degree C combustion catalytic oxidation method has been adopted for the TOC-L series.
http://www.shimadzu.com/an/toc/lab/toc-l2.html Community Standard Description: http://onto.nerc.ac.uk/CAST/124.html