http://lod.bco-dmo.org/id/dataset/819975
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Silica and nitrogen analyses from incubation experiments conducted using seagrass cores from 1m depth in Grand Bay in 2017.
2020-08-10
publication
2020-08-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-11
publication
https://doi.org/10.26008/1912/bco-dmo.819975.1
Jeffrey W. Krause
Dauphin Island Sea Lab
principalInvestigator
Just Cebrian
Mississippi State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Krause, J. (2020) Silica and nitrogen analyses from incubation experiments conducted using seagrass cores from 1m depth in Grand Bay in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.819975.1 [access date]
Methods and Sampling: <p>Three repeated experimental trials were done in summer months. Thirty-two cores (27 cm diameter, 14 cm depth) were collected from 50 m2 area of seagrass bed at 1 m depth on: June 28, July 12 and July 26, 2017 for trials 1-3, respectively. On each date, 16 cores were collected from seagrass habitat in pairs. Another 16 cores were collected from open sediment (OS) habitat. Extracted, paired cores were placed upright into an open-top plastic tub (49 x 33 x 42 cm) to produce eight tubs of each habitat.</p>
<p>Tubs were transported to Dauphin Island Sea Lab (~30-minute drive) filled with seawater (to core depth of 16 cm) pumped from Mobile Bay (20 km, east of site) and arranged in four blocks within an outdoor mesocosm. Each block contained two tubs of each habitat. After two days, a diatom-specific inhibitor (3 µM solution of germanic acid, i.e. Ge treatment) was randomly added to water, i.e. two tubs per block, one of each habitat type. Germanium (Ge) at high Ge/Si ratios (&gt; 0.01) prevents formation of siliceous cell wall (Azam and Chisholm 1976). We added 3 µM solution and allowed two days for Ge incorporation.</p>
<p>Metabolism measurements:</p>
<p>Two days after, we quantified productivity and respiration from changes in oxygen content within 2-3 hour incubations of chambers and bottles following methods in Anton et al. (2009).</p>
<p>Nitrogen and Silica:</p>
<p>At end of incubation, 100 mL of water from each clear chamber was filtered through 47 mm Whatman glass fiber filter. Filtered water was analyzed for total dissolved nitrogen (TDN) and nitrate+nitrite (NO3-+NO2-) colorimetrically using Skalar autoanalyzer (Dzwonkowski et al. 2017), and for dissolved silicic acid (Si(OH)4) using a manual colorimetric method (Krause et al. 2009).</p>
<p>Statistical analyses:</p>
<p>A series of two-way ANOVAs with trial and treatment as fixed factors were used to test for differences in environment in both habitats.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1558957 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1558957
completed
Jeffrey W. Krause
Dauphin Island Sea Lab
251-861-2141 x2289
101 Bienville Blvd
Dauphin Island
AL
36528
USA
jkrause@disl.edu
pointOfContact
Just Cebrian
Mississippi State University
251-423-4994
jcebrian@ngi.msstate.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Analysis_ID
Sample_ID
Collection_Date
Ge_Addition_Date
Experimental_Trial_Date
Trial
Core_Taken_From
Ge_Control_Treatment
Seagrass_Sediment_Habitat
Light_Dark_Incubation
NO3_NO2
NO2
NH4
TDN
SRP
Dsi
HQ30d, Hach, Loveland, Colorado, USA
Skalar autoanalyzer
theme
None, User defined
sample identification
date
experiment type
treatment
nitrate plus nitrite
Nitrite
Ammonium
Total Dissolved Nitrogen
SRP
silica
featureType
BCO-DMO Standard Parameters
Multi Parameter Portable Meter
Nutrient Autoanalyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The biotic and abiotic controls on the Silicon cycle in the northern Gulf of Mexico
https://osprey.bco-dmo.org/project/712667
The biotic and abiotic controls on the Silicon cycle in the northern Gulf of Mexico
<p><em>NSF Award Abstract:</em><br />
The Louisiana Shelf system in the northern Gulf of Mexico is fed by the Mississippi River and its many tributaries which contribute large quantities of nutrients from agricultural fertilizer to the region. Input of these nutrients, especially nitrogen, has led to eutrophication. Eutrophication is the process wherein a body of water such as the Louisiana Shelf becomes enriched in dissolved nutrients that increase phytoplankton growth which eventually leads to decreased oxygen levels in bottom waters. This has certainly been observed in this area, and diatoms, a phytoplankton which represents the base of the food chain, have shown variable silicon/nitrogen (Si/N) ratios. Because diatoms create their shells from silicon, their growth is controlled not only by nitrogen inputs but the availability of silicon. Lower Si/N ratios are showing that silicon may be playing an increasingly important role in regulating diatom production in the system. For this reason, a scientist from the University of South Alabama will determine the biogeochemical processes controlling changes in Si/N ratios in the Louisiana Shelf system. One graduate student on their way to a doctorate degree and three undergraduate students will be supported and trained as part of this project. Also, four scholarships for low-income, high school students from Title 1 schools will get to participate in a month-long summer Marine Science course at the Dauphin Island Sea Laboratory and be included in the research project. The study has significant societal benefits given this is an area where $2.4 trillion gross domestic product revenue is tied up in coastal resources. Since diatoms are at the base of the food chain that is the biotic control on said coastal resources, the growth of diatoms in response to eutrophication is important to study.</p>
<p>Eutrophication of the Mississippi River and its tributaries has the potential to alter the biological landscape of the Louisiana Shelf system in the northern Gulf of Mexico by influencing the Si/N ratios below those that are optimal for diatom growth. A scientist from the University of South Alabama believes the observed changes in the Si/N ratio may indicate silicon now plays an important role in regulating diatom production in the system. As such, understanding the biotic and abiotic processes controlling the silicon cycle is crucial because diatoms dominate at the base of the food chain in this highly productive region. The study will focus on following issues: (1) the importance of recycled silicon sources on diatom production; (2) can heavily-silicified diatoms adapt to changing Si/N ratios more effectively than lightly-silicified diatoms; and (3) the role of reverse weathering in sequestering silicon thereby reducing diffusive pore-water transport. To attain these goals, a new analytical approach, the PDMPO method (compound 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylamino-carbamoyl)methoxy)phenyl)oxazole) that quantitatively measures taxa-specific silica production would be used.</p>
CLASiC
largerWorkCitation
project
eng; USA
oceans
-88.312561
-88.312561
30.383174
30.383174
2017-06-28
2017-07-31
Northern Gulf of Mexico, specifically the Louisiana Shelf region dominated by the discharge of the Mississippi River on the western side of the delta
0
BCO-DMO catalogue of parameters from Silica and nitrogen analyses from incubation experiments conducted using seagrass cores from 1m depth in Grand Bay in 2017.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/820345.rdf
Name: Analysis_ID
Units: unitless
Description: <p>name given to the water sample for analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/820346.rdf
Name: Sample_ID
Units: unitless
Description: <p>name of the core water came from</p>
http://lod.bco-dmo.org/id/dataset-parameter/820347.rdf
Name: Collection_Date
Units: yyyy-mm-dd
Description: <p>date the core was collected from field site</p>
http://lod.bco-dmo.org/id/dataset-parameter/820348.rdf
Name: Ge_Addition_Date
Units: yyyy-mm-dd
Description: <p>date Ge was added to cores</p>
http://lod.bco-dmo.org/id/dataset-parameter/820349.rdf
Name: Experimental_Trial_Date
Units: yyyy-mm-dd
Description: <p>date of the incubation</p>
http://lod.bco-dmo.org/id/dataset-parameter/820350.rdf
Name: Trial
Units: unitless
Description: <p>indicates whether it is trial 1, 2, or 3</p>
http://lod.bco-dmo.org/id/dataset-parameter/820351.rdf
Name: Core_Taken_From
Units: unitless
Description: <p>indicates where the core was taken from</p>
http://lod.bco-dmo.org/id/dataset-parameter/820352.rdf
Name: Ge_Control_Treatment
Units: unitless
Description: <p>indicates whether the sample came from a core given Ge or a control (no Ge added)</p>
http://lod.bco-dmo.org/id/dataset-parameter/820353.rdf
Name: Seagrass_Sediment_Habitat
Units: unitless
Description: <p>indicates whether the core the sample came from was collected from a seagrass or sediment habitat</p>
http://lod.bco-dmo.org/id/dataset-parameter/820354.rdf
Name: Light_Dark_Incubation
Units: unitless
Description: <p>indicates whether the core the sample came from was incubated in a clear or a darkened container</p>
http://lod.bco-dmo.org/id/dataset-parameter/820355.rdf
Name: NO3_NO2
Units: uM
Description: <p>NO3+NO2 value of the water sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/820356.rdf
Name: NO2
Units: uM
Description: <p>NO2 value of the water sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/820357.rdf
Name: NH4
Units: uM
Description: <p>NH4 value of the water sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/820358.rdf
Name: TDN
Units: uM
Description: <p>Total dissolved nitrogen in the water sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/820359.rdf
Name: SRP
Units: micromole per liter (umol/L)
Description: <p>soluble reactive phosphorous in the water sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/820360.rdf
Name: Dsi
Units: micromole per liter (umol/L)
Description: <p>dissolved silica in the water sample</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5452
https://darchive.mblwhoilibrary.org/bitstream/1912/26058/1/dataset-819975_diatom-contribution-benthic-gpp-silica-and-nitrogen-analyses__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.819975.1
download
onLine
dataset
<p>Three repeated experimental trials were done in summer months. Thirty-two cores (27 cm diameter, 14 cm depth) were collected from 50 m2 area of seagrass bed at 1 m depth on: June 28, July 12 and July 26, 2017 for trials 1-3, respectively. On each date, 16 cores were collected from seagrass habitat in pairs. Another 16 cores were collected from open sediment (OS) habitat. Extracted, paired cores were placed upright into an open-top plastic tub (49 x 33 x 42 cm) to produce eight tubs of each habitat.</p>
<p>Tubs were transported to Dauphin Island Sea Lab (~30-minute drive) filled with seawater (to core depth of 16 cm) pumped from Mobile Bay (20 km, east of site) and arranged in four blocks within an outdoor mesocosm. Each block contained two tubs of each habitat. After two days, a diatom-specific inhibitor (3 µM solution of germanic acid, i.e. Ge treatment) was randomly added to water, i.e. two tubs per block, one of each habitat type. Germanium (Ge) at high Ge/Si ratios (&gt; 0.01) prevents formation of siliceous cell wall (Azam and Chisholm 1976). We added 3 µM solution and allowed two days for Ge incorporation.</p>
<p>Metabolism measurements:</p>
<p>Two days after, we quantified productivity and respiration from changes in oxygen content within 2-3 hour incubations of chambers and bottles following methods in Anton et al. (2009).</p>
<p>Nitrogen and Silica:</p>
<p>At end of incubation, 100 mL of water from each clear chamber was filtered through 47 mm Whatman glass fiber filter. Filtered water was analyzed for total dissolved nitrogen (TDN) and nitrate+nitrite (NO3-+NO2-) colorimetrically using Skalar autoanalyzer (Dzwonkowski et al. 2017), and for dissolved silicic acid (Si(OH)4) using a manual colorimetric method (Krause et al. 2009).</p>
<p>Statistical analyses:</p>
<p>A series of two-way ANOVAs with trial and treatment as fixed factors were used to test for differences in environment in both habitats.</p>
Specified by the Principal Investigator(s)
<p>Excel, Sigma Plot</p>
<p>BCO-DMO Data Manager Processing Notes:<br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* blank values in this dataset are displayed as "nd" for "no data."&nbsp; nd is the default missing data identifier in the BCO-DMO system. Added ND as a missing data identifier.<br />
* removed all spaces in headers and replaced with underscores<br />
* removed all units from headers<br />
* converted dates to ISO Format yyyy-mm-dd<br />
* set Types for each data column&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
HQ30d, Hach, Loveland, Colorado, USA
HQ30d, Hach, Loveland, Colorado, USA
PI Supplied Instrument Name: HQ30d, Hach, Loveland, Colorado, USA Instrument Name: Multi Parameter Portable Meter Instrument Short Name: Instrument Description: An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held.
Skalar autoanalyzer
Skalar autoanalyzer
PI Supplied Instrument Name: Skalar autoanalyzer Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/