http://lod.bco-dmo.org/id/dataset/820462
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Growth of Antarctic krill (Euphausia superba) from an experiment at two temperatures and three pH levels, Feb-Mar. 2015
2020-08-10
publication
2020-08-10
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-24
publication
https://doi.org/10.26008/1912/bco-dmo.820462.1
Grace Saba
Rutgers University
principalInvestigator
Brad Seibel
University of South Florida
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Saba, G., Seibel, B. (2020) Growth of Antarctic krill (Euphausia superba) from an experiment at two temperatures and three pH levels, Feb-Mar. 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.820462.1 [access date]
2015 krill growth expt 2 Dataset Description: <p>Antarctic krill (Euphausia superba) growth data collected during an experiment where krill were allowed to molt once in ambient conditions then placed in individual bottles maintained at one of six target treatments: 1) Ambient temperature (0 degrees C) and ambient pH (8.00); 2) Ambient temperature (0 degrees C) and pH = 7.50; 3) Ambient temperature (0 degrees C) and pH = 7.10; 4) Elevated temperature (3 degrees C) and ambient pH (8.00); 5) Elevated temperature (3 degrees C) and pH = 7.50; 6) Elevated temperature (3&nbsp; degrees C) and pH = 7.10. The bottles were incubated until the either the krill molted a second time or the experiment ended (after 26 days).</p> Methods and Sampling: <p><strong>Sampling and analytical procedures:</strong></p>
<p><strong>Capture and husbandry:</strong> Antarctic krill (Euphausia superba) were captured during the austral summer of 2014/2015. Krill were collected by net tow (2 m diameter, 1000 m mesh, non-filtering cod end) off the R/V Laurence M. Gould near the Western Antarctic Peninsula and transported directly to the Palmer Station biological laboratory. One to two thousand krill were housed in one 4'w x 3'h circular holding tank and two 5’ x 2’ x 1’ rectangular tanks provided with aeration and flow-through seawater. Water was non-filtered and individuals were able to feed on plankton ad libitum throughout the season.</p>
<p><strong>Experimental treatments:</strong> Six experimental treatments were targeted in this study: 1) Ambient temperature (0 degrees C) and ambient pH (8.00); 2) Ambient temperature (0 degrees C) and pH = 7.50; 3) Ambient temperature (0 degrees C) and pH = 7.10; 4) Elevated temperature (3 degrees C) and ambient pH (8.00); 5) Elevated temperature (3 degrees C) and pH = 7.50; 6) Elevated temperature (3&nbsp; degrees C) and pH = 7.10. Temperature treatments were obtained using two separate recirculating systems. Three 800 L cylindrical polycarbonate carboys were attached to temperature controlled chillers (Delta Star) and inline pumps. The carboys were filled with non-filtered seawater acquired from the Palmer Station intake line, placed in a flow-through water bath, and maintained at 0&nbsp;degrees C. Three other 800 L carboys were set up without a chiller and placed in an environmental chamber at 3 degrees C. The systems were replaced with new water daily and allowed to acclimate to temperature for a minimum of 24 hours before the start of a trial or water change. High CO2 conditions were obtained using a peristaltic pump to inject straight CO2 into the propeller of a pump submerged in seawater. Treated water was then gently siphoned with minimal disturbance into treatment bottles.</p>
<p>Krill were picked and placed in individual 4 L wide-mouth polycarbonate bottles with airtight lids (n = 26 per experimental treatment). Bottles were filled with ambient seawater. Bottles were immediately closed and placed in environmental chambers at ambient temperature (0&nbsp;degrees C). Every 24-48 hours, 80% of the water was siphoned out of each bottle and replaced with new ambient seawater to minimize excretory and respiratory effect. Bottles were checked for molts twice per day. If a molt was observed, the molt was removed for processing (measured for krill uropod and total length), and the krill was placed in another individual 4 L wide-mouth polycarbonate bottle containing one of the six pre-acclimated treatment waters as described above to begin the second phase of the experiment. The bottle was then immediately closed and placed in environmental chambers at either 0&nbsp;or 3&nbsp;degrees C. This process was repeated for all the krill after each molted for the first time, enabling the experiment to continue with a total of 26 krill per treatment. Water changes every 24-48 hours with either ambient seawater (for krill awaiting first molt) or with pre-acclimated treatment water (for krill awaiting second molt) and twice daily checks for molts continued for the duration of the experiment. If a second molt was observed, that bottle was pulled from the experiment and the molt and live krill processed. The experiment ended on March 7 due to the end of the Palmer Station field season, and a majority of the krill did not molt a second time. However, the remaining krill were processed for uropod and total length and wet weight.</p>
<p><strong>Analyses:</strong> Upon the first molting event for each krill, the molt was gently removed from the bottle. The molt was measured for uropod and total length (mm) with digital calipers (Fowler).&nbsp;</p>
<p>Once a krill molted a second time, both the krill and molt were gently removed from the bottle. Both the molt and krill were measured for uropod and total length (mm) with digital calipers (Fowler), and the krill wet weight (mg) was measured. At the end of the experiment, the remaining krill that did not molt a second time were processed for uropod and total length (mm) and wet weight (mg).&nbsp;</p>
<p>pH was measured in each experimental bottle at the start of the experiment. Salinity, pH, and total alkalinity were measured during water changes. The water changes occurred every 24-48 hours, but we were unable to collect salinity, pH, and total alkalinity samples at every water change due to time constraints. Salinity was measured with a bench top conductivity meter (YSI 3100) calibrated daily with a conductivity standard (50,000 uS/cm; Ricca Chemical Company). pH was determined spectrophotometrically using the indicator dye thymol blue (Dickson et al. 2007; Zhang and Byrne 1996). Total alkalinity was determined on 100 ml subsamples with an open-cell, potentiometric titration of seawater (Metrohm 888 Titrando) with 0.1 M HCl following the potential of a pH electrode (Dickson et al. 2007). Tiamo software (version 2.3) was used to process the alkalinity data. Measurements of pH and TA were quality controlled using certified reference materials (CRMs) obtained from Andrew Dickson at UCSD Scripps Institute of Oceanography</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1641198 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1641198
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1246293 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1246293
completed
Grace Saba
Rutgers University
848-932-3466
71 Dudley Rd Room 316
New Brunswick
NJ
08901
US
saba@marine.rutgers.edu
pointOfContact
Brad Seibel
University of South Florida
401-744-4927
College of Marine Science 830 1st St SE
St. Petersburg
FL
33701
USA
seibel@usf.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Bottle_Krill
Treatment_temp
Treatment_Target_pH
Expt_Start_Date
First_Molt_Date
Duration_to_first_molt
First_molt_uropod_length
Total_krill_length_pre_molt
Second_Molt_Date
Duration_between_first_and_second_molt
Second_molt_uropod_length
Total_krill_length_after_first_molt
Live_krill_uropod_length
Live_krill_total_length
Krill_wet_weight
Notes
Delta Star chiller
Fowler digital calipers
YSI 3100 Conductivity Instrument
Shimadzu spectrophotometer
Metrohm 888 Titrando
theme
None, User defined
sample identification
water temperature at measurement depth
pH
date_start
date end
duration
length
date
weight
comments
featureType
BCO-DMO Standard Parameters
Aquarium chiller
calipers
Conductivity Meter
Plankton Net
Spectrophotometer
Titrator
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Synergistic effects of Elevated Carbon Dioxide (CO2) and Temperature on the Metabolism, Growth, and Reproduction of Antarctic Krill (Euphausia superba)
http://coseenow.net/project-parka/
Collaborative Research: Synergistic effects of Elevated Carbon Dioxide (CO2) and Temperature on the Metabolism, Growth, and Reproduction of Antarctic Krill (Euphausia superba)
<p><em>NSF Award Abstract:</em><br />
Climate change projections for this century suggest that the Southern Ocean will be the first region to be affected by seawater chemistry changes associated with enhanced carbon dioxide (CO2). Additionally, regions of the Southern Ocean are warming faster than any other locations on the planet. Ocean acidification and warming may act synergistically to impair the performance of different organisms by simultaneously increasing metabolic needs and reducing oxygen transport. However, no studies have measured krill acid-base regulation, metabolism, growth, or reproduction in the context of ocean acidification or synergistic ?greenhouse? conditions of elevated CO2 and temperature. In the present project, the investigators will conduct both short and prolonged exposure experiments at Palmer Station, Antarctica to determine the responses of Euphausia superba to elevated CO2 and temperature. The investigators will test hypotheses related to acid-base compensation and acclimation of various life stages of krill to elevated CO2 and temperature. Furthermore, they will determine these impacts on feeding, respiration, metabolism, growth, and reproduction.</p>
<p>The Antarctic krill, Euphausia superba, is a key species in Antarctic food webs as they are a primary food source for many of the top predators in the Southern Ocean including baleen whales, seals, penguins, and other sea birds. This project will determine the responses of Antarctic krill exposed to elevated CO2 and temperature and whether or not krill have the capacity to fully compensate under future ocean conditions. The proposed field effort will be complemented by an extensive broader impact effort focused on bringing marine science to both rural and urban high school students in the Midwest (Kansas). The core educational objectives of this proposal are to 1) instruct students about potential careers in marine science, 2) engage students and promote their interest in the scientific process, critical thinking, and applications of science, mathematics, and technology, and 3) and increase student and teacher awareness and understanding of the oceans and global climate change, with special focus on the Western Antarctic Peninsula region. Finally, this project will engage undergraduate and graduate students in the production, analysis, presentation and publication of datasets.</p>
OA Krill
largerWorkCitation
project
eng; USA
biota
oceans
-64.0526
-64.0526
-64.7741
-64.7741
2015-02-09
2015-03-05
Palmer Station, Antarctica
0
BCO-DMO catalogue of parameters from Growth of Antarctic krill (Euphausia superba) from an experiment at two temperatures and three pH levels, Feb-Mar. 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/820715.rdf
Name: Bottle_Krill
Units: unitless
Description: <p>bottle number</p>
http://lod.bco-dmo.org/id/dataset-parameter/820716.rdf
Name: Treatment_temp
Units: unitless
Description: <p>Temperature treatment; either Ambient (0 degrees C) or Elevated (3 degrees C)</p>
http://lod.bco-dmo.org/id/dataset-parameter/820717.rdf
Name: Treatment_Target_pH
Units: unitless
Description: <p>Targeted pH treatment; either 8.00; 7.50; or 7.10</p>
http://lod.bco-dmo.org/id/dataset-parameter/820718.rdf
Name: Expt_Start_Date
Units: unitless
Description: <p>Date experiment was begun; ISO formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/820719.rdf
Name: First_Molt_Date
Units: unitless
Description: <p>Date krill molted in bottle at ambient conditions; krill transferred to bottle under experimental treatments experiment on this date; ISO formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/820720.rdf
Name: Duration_to_first_molt
Units: days
Description: <p>Length of time between start of experiment and time of first molt</p>
http://lod.bco-dmo.org/id/dataset-parameter/820721.rdf
Name: First_molt_uropod_length
Units: mm
Description: <p>Krill uropod length determined from the first molt</p>
http://lod.bco-dmo.org/id/dataset-parameter/820722.rdf
Name: Total_krill_length_pre_molt
Units: mm
Description: <p>Krill length determined from the first molt</p>
http://lod.bco-dmo.org/id/dataset-parameter/820723.rdf
Name: Second_Molt_Date
Units: unitless
Description: <p>Date krill molted for second time; under treatment conditions; bottle pulled from experiment on this date; ISO formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/820724.rdf
Name: Duration_between_first_and_second_molt
Units: days
Description: <p>Length of time between first and second molts</p>
http://lod.bco-dmo.org/id/dataset-parameter/820725.rdf
Name: Second_molt_uropod_length
Units: mm
Description: <p>Krill uropod length determined from the second molt</p>
http://lod.bco-dmo.org/id/dataset-parameter/820726.rdf
Name: Total_krill_length_after_first_molt
Units: mm
Description: <p>Krill length determined from the second molt</p>
http://lod.bco-dmo.org/id/dataset-parameter/820727.rdf
Name: Live_krill_uropod_length
Units: mm
Description: <p>Krill uropod length determined from the live krill after either it molted a second time or at the end of the experiment; whichever came first</p>
http://lod.bco-dmo.org/id/dataset-parameter/820728.rdf
Name: Live_krill_total_length
Units: mm
Description: <p>Krill length determined from the live krill after either it molted a second time or at the end of the experiment; whichever came first</p>
http://lod.bco-dmo.org/id/dataset-parameter/820729.rdf
Name: Krill_wet_weight
Units: mg
Description: <p>Wet weight determined from the live krill after either it molted a second time or at the end of the experiment; whichever came first</p>
http://lod.bco-dmo.org/id/dataset-parameter/820730.rdf
Name: Notes
Units: unitless
Description: <p>comments on experiment</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2387
https://darchive.mblwhoilibrary.org/bitstream/1912/26112/1/2015-2_carbonate.csv
download
18349
https://darchive.mblwhoilibrary.org/bitstream/1912/26112/2/dataset-820462_2015-antarctic-krill-growth-experiment-2__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.820462.1
download
onLine
dataset
<p><strong>Sampling and analytical procedures:</strong></p>
<p><strong>Capture and husbandry:</strong> Antarctic krill (Euphausia superba) were captured during the austral summer of 2014/2015. Krill were collected by net tow (2 m diameter, 1000 m mesh, non-filtering cod end) off the R/V Laurence M. Gould near the Western Antarctic Peninsula and transported directly to the Palmer Station biological laboratory. One to two thousand krill were housed in one 4'w x 3'h circular holding tank and two 5’ x 2’ x 1’ rectangular tanks provided with aeration and flow-through seawater. Water was non-filtered and individuals were able to feed on plankton ad libitum throughout the season.</p>
<p><strong>Experimental treatments:</strong> Six experimental treatments were targeted in this study: 1) Ambient temperature (0 degrees C) and ambient pH (8.00); 2) Ambient temperature (0 degrees C) and pH = 7.50; 3) Ambient temperature (0 degrees C) and pH = 7.10; 4) Elevated temperature (3 degrees C) and ambient pH (8.00); 5) Elevated temperature (3 degrees C) and pH = 7.50; 6) Elevated temperature (3&nbsp; degrees C) and pH = 7.10. Temperature treatments were obtained using two separate recirculating systems. Three 800 L cylindrical polycarbonate carboys were attached to temperature controlled chillers (Delta Star) and inline pumps. The carboys were filled with non-filtered seawater acquired from the Palmer Station intake line, placed in a flow-through water bath, and maintained at 0&nbsp;degrees C. Three other 800 L carboys were set up without a chiller and placed in an environmental chamber at 3 degrees C. The systems were replaced with new water daily and allowed to acclimate to temperature for a minimum of 24 hours before the start of a trial or water change. High CO2 conditions were obtained using a peristaltic pump to inject straight CO2 into the propeller of a pump submerged in seawater. Treated water was then gently siphoned with minimal disturbance into treatment bottles.</p>
<p>Krill were picked and placed in individual 4 L wide-mouth polycarbonate bottles with airtight lids (n = 26 per experimental treatment). Bottles were filled with ambient seawater. Bottles were immediately closed and placed in environmental chambers at ambient temperature (0&nbsp;degrees C). Every 24-48 hours, 80% of the water was siphoned out of each bottle and replaced with new ambient seawater to minimize excretory and respiratory effect. Bottles were checked for molts twice per day. If a molt was observed, the molt was removed for processing (measured for krill uropod and total length), and the krill was placed in another individual 4 L wide-mouth polycarbonate bottle containing one of the six pre-acclimated treatment waters as described above to begin the second phase of the experiment. The bottle was then immediately closed and placed in environmental chambers at either 0&nbsp;or 3&nbsp;degrees C. This process was repeated for all the krill after each molted for the first time, enabling the experiment to continue with a total of 26 krill per treatment. Water changes every 24-48 hours with either ambient seawater (for krill awaiting first molt) or with pre-acclimated treatment water (for krill awaiting second molt) and twice daily checks for molts continued for the duration of the experiment. If a second molt was observed, that bottle was pulled from the experiment and the molt and live krill processed. The experiment ended on March 7 due to the end of the Palmer Station field season, and a majority of the krill did not molt a second time. However, the remaining krill were processed for uropod and total length and wet weight.</p>
<p><strong>Analyses:</strong> Upon the first molting event for each krill, the molt was gently removed from the bottle. The molt was measured for uropod and total length (mm) with digital calipers (Fowler).&nbsp;</p>
<p>Once a krill molted a second time, both the krill and molt were gently removed from the bottle. Both the molt and krill were measured for uropod and total length (mm) with digital calipers (Fowler), and the krill wet weight (mg) was measured. At the end of the experiment, the remaining krill that did not molt a second time were processed for uropod and total length (mm) and wet weight (mg).&nbsp;</p>
<p>pH was measured in each experimental bottle at the start of the experiment. Salinity, pH, and total alkalinity were measured during water changes. The water changes occurred every 24-48 hours, but we were unable to collect salinity, pH, and total alkalinity samples at every water change due to time constraints. Salinity was measured with a bench top conductivity meter (YSI 3100) calibrated daily with a conductivity standard (50,000 uS/cm; Ricca Chemical Company). pH was determined spectrophotometrically using the indicator dye thymol blue (Dickson et al. 2007; Zhang and Byrne 1996). Total alkalinity was determined on 100 ml subsamples with an open-cell, potentiometric titration of seawater (Metrohm 888 Titrando) with 0.1 M HCl following the potential of a pH electrode (Dickson et al. 2007). Tiamo software (version 2.3) was used to process the alkalinity data. Measurements of pH and TA were quality controlled using certified reference materials (CRMs) obtained from Andrew Dickson at UCSD Scripps Institute of Oceanography</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- data submitted in Excel file "2015_AntarcticKrill_GrowthExperiment_1.xlsx" sheet "Sheet1" extracted to csv<br />
-&nbsp;removed carbonate table to server separately<br />
-&nbsp;added conventional header with dataset name, PI name, version date<br />
-&nbsp;modified parameter names to conform with BCO-DMO naming conventions<br />
- converted dates to ISO format (yyyy-mm-dd)<br />
- replaced ND with nd for 'no data'</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Delta Star chiller
Delta Star chiller
PI Supplied Instrument Name: Delta Star chiller PI Supplied Instrument Description:Used to cool water to ambient temperature. Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.
Fowler digital calipers
Fowler digital calipers
PI Supplied Instrument Name: Fowler digital calipers PI Supplied Instrument Description:Used to measure krill uropod and total lengths. Instrument Name: calipers Instrument Short Name: Instrument Description: A caliper (or "pair of calipers") is a device used to measure the distance between two opposite sides of an object. Many types of calipers permit reading out a measurement on a ruled scale, a dial, or a digital display.
YSI 3100 Conductivity Instrument
YSI 3100 Conductivity Instrument
PI Supplied Instrument Name: YSI 3100 Conductivity Instrument PI Supplied Instrument Description:Used to measure salinity in seawater. Instrument Name: Conductivity Meter Instrument Short Name:Conductivity Meter Instrument Description: Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.
PI Supplied Instrument Name: PI Supplied Instrument Description:Net with 2 m diameter, 1000 m mesh, non-filtering cod end. Used to collected krill for experimental analyses. Instrument Name: Plankton Net Instrument Short Name:Plankton Net Instrument Description: A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/22/
Shimadzu spectrophotometer
Shimadzu spectrophotometer
PI Supplied Instrument Name: Shimadzu spectrophotometer PI Supplied Instrument Description:Used to measure pH in seawater. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Metrohm 888 Titrando
Metrohm 888 Titrando
PI Supplied Instrument Name: Metrohm 888 Titrando PI Supplied Instrument Description:Instrument used for open-cell titrations to determine total alkalinity in seawater. Used to measure total alkalinity in seawater. Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/