http://lod.bco-dmo.org/id/dataset/820557
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Data from two Antarctic krill (Euphausia superba) 24-hour feeding experiments under ambient temperature and pCO2, ambient temperature and elevated pCO2, and elevated temperature and pCO2.
2020-08-12
publication
2020-08-12
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-24
publication
https://doi.org/10.26008/1912/bco-dmo.820557.1
Grace Saba
Rutgers University
principalInvestigator
Brad Seibel
University of South Florida
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Saba, G., Seibel, B. (2020) Data from two Antarctic krill (Euphausia superba) 24-hour feeding experiments under ambient temperature and pCO2, ambient temperature and elevated pCO2, and elevated temperature and pCO2. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.820557.1 [access date]
2014 krill growth Dataset Description: <p>Data from two Antarctic krill (<em>Euphausia superba</em>) 24-hour feeding experiments under ambient temperature and pCO2, ambient temperature and elevated pCO2, and elevated temperature and pCO2.</p>
<p>We conducted perturbation experiments to determine potential changes in feeding rates of Euphausia superba (32-41 mm) due to decreased pH and elevated temperature. Target pH was reached in the experiments via CO2 bubbling of seawater flowing through gas equilibration columns. The two feeding experiments differed in acclimation time.&nbsp; Krill in experiment 1 (Exp 1) and experiment 2 (Exp 2) were acclimated to treatment conditions for 48 hours and 21 days, respectively.</p> Methods and Sampling: <p><strong>Sampling and analytical procedures:&nbsp;</strong></p>
<p><strong>Capture and husbandry:</strong> Antarctic krill (Euphausia superba) were captured during the austral summer of 2013/2014. Krill were collected by net tow (2 m diameter, 1000 m mesh, non-filtering cod end) off the R/V Laurence M. Gould near the Western Antarctic Peninsula and transported directly to the Palmer Station biological laboratory. One to two thousand krill were housed in one 4'w x 3'h circular holding tank and two 5’ x 2’ x 1’ rectangular tanks provided with aeration and flow-through seawater. Water was non-filtered and individuals were able to feed on plankton ad libitum throughout the season.</p>
<p><strong>Experimental treatments:</strong> Three experimental treatments were targeted in this study: (1) ambient temperature and ambient pCO2/pH (400ppm/8.10), (2) ambient temperature and elevated pCO2 (800ppm)/reduced pH (7.7), and (3) elevated temperature (3 degrees C) and elevated pCO2 (800ppm)/reduced pH (7.7). Two replicate feeding experiments were conducted: Experiment 1 and 2. Temperature treatments were obtained using two separate recirculating systems. Two 800 L cylindrical polycarbonate carboys were attached to temperature controlled chillers (Delta Star) and inline pumps. The carboys were filled with non-filtered seawater acquired from the Palmer Station intake line, placed in a flow-through water bath, and maintained at 0&nbsp;degrees C. Another 800 L carboy was set up without a chiller and placed in an environmental chamber set at bated for about 24 hours before sacrificed for sampling the end points. The samples collected in the bottles at T0 and Tfinal include: pH, total alkalinity, and fluorometric chlorophyll a.&nbsp;</p>
<p>The systems were replaced with new water daily and allowed to acclimate to temperature for a minimum of 24 hours before the start of a trial or water change. High CO2 conditions were obtained using a peristaltic pump to inject straight CO2 into the propeller of a pump submerged in seawater. Treated water was then gently siphoned with minimal disturbance into treatment bottles.&nbsp;</p>
<p>For each of the three treatments, 14, 4 L wide-mouth polycarbonate bottles were filled with the appropriate equilibrated seawater. Two bottles per treatment served as T0 controls (no krill added) and were sacrificed for an initial suite of samples. Two bottles served as Tfinal (24 h) controls, and one juvenile krill was added to each of the remaining 10 bottles per treatment (Tfinal treatments). The Tfinal bottles were capped to maintain target pCO2/pH, incubated in the appropriate location to maintain desired temperature (water bath for ambient, 0&nbsp;degrees C; 3 degrees C environmental chamber for elevated temperature), and incubated for about 24 hours before sacrificed for sampling the end points. The samples collected in the bottles at T0 and Tfinal include: pH, total alkalinity, and fluorometric chlorophyll a.</p>
<p><strong>Analyses:</strong>&nbsp; pH was determined spectrophotometrically using the indicator dye thymol blue (Dickson et al. 2007; Zhang and Byrne 1996). Total alkalinity was determined on 100 ml subsamples with an open-cell, potentiometric titration of seawater (Metrohm 888 Titrando) with 0.1 M HCl following the potential of a pH electrode (Dickson et al. 2007). Tiamo software (version 2.3) was used to process the alkalinity data. Measurements of pH and TA were quality controlled using certified reference materials (CRMs) obtained from Andrew Dickson at UCSD Scripps Institute of Oceanography. An aliquot of seawater from each incubation bottle was also filtered onto a GF/F filter, which was wrapped in foil and frozen for fluorometric chlorophyll a&nbsp; analysis (Parsons et al. 1984).&nbsp;Salinity was measured with a bench top conductivity meter (YSI 3100) calibrated daily with a conductivity standard (50,000 uS/cm; Ricca Chemical Company).</p>
<table border="0" cellpadding="0" cellspacing="0" style="width:515px">
<tbody>
<tr>
<td style="height:21px; width:312px"><strong>Expt 1</strong></td>
<td style="width:203px"><strong>Expt 2</strong></td>
</tr>
<tr>
<td style="height:21px">Salinity (ave +/- sterr; n = 30) = 32.43 +/- 0.02</td>
<td>Salinity (ave +/- sterr; n = 30) = 32.63 +/- 0.03</td>
</tr>
<tr>
<td style="height:21px">Ambient temperature = 0 C</td>
<td>Ambient temperature = 0 C</td>
</tr>
<tr>
<td style="height:21px">Elevated Temperature = 3 C</td>
<td>Elevated Temperature = 3 C</td>
</tr>
</tbody>
</table>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1641198 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1641198
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1246293 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1246293
completed
Grace Saba
Rutgers University
848-932-3466
71 Dudley Rd Room 316
New Brunswick
NJ
08901
US
saba@marine.rutgers.edu
pointOfContact
Brad Seibel
University of South Florida
401-744-4927
College of Marine Science 830 1st St SE
St. Petersburg
FL
33701
USA
seibel@usf.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Feeding_Experiment
Expt_Start_Date
Bottle_description
Time
Treatment_temp
Treatment_pCO2
Incubation_time
spec_pH
Total_akalinity
Chlorophyll_a
Notes
Delta Star chiller
YSI 3100 Conductivity Instrument
Shimadzu spectrophotometer
Metrohm 888 Titrando
Turner 10 AU Fluorometer
theme
None, User defined
experiment id
date_start
treatment
No BCO-DMO term
water temperature at measurement depth
Partial pressure of CO2
incubation time or duration
pH
total alkalinity (TA)
chlorophyll a
comments
featureType
BCO-DMO Standard Parameters
Aquarium chiller
Conductivity Meter
Plankton Net
Spectrophotometer
Titrator
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Synergistic effects of Elevated Carbon Dioxide (CO2) and Temperature on the Metabolism, Growth, and Reproduction of Antarctic Krill (Euphausia superba)
http://coseenow.net/project-parka/
Collaborative Research: Synergistic effects of Elevated Carbon Dioxide (CO2) and Temperature on the Metabolism, Growth, and Reproduction of Antarctic Krill (Euphausia superba)
<p><em>NSF Award Abstract:</em><br />
Climate change projections for this century suggest that the Southern Ocean will be the first region to be affected by seawater chemistry changes associated with enhanced carbon dioxide (CO2). Additionally, regions of the Southern Ocean are warming faster than any other locations on the planet. Ocean acidification and warming may act synergistically to impair the performance of different organisms by simultaneously increasing metabolic needs and reducing oxygen transport. However, no studies have measured krill acid-base regulation, metabolism, growth, or reproduction in the context of ocean acidification or synergistic ?greenhouse? conditions of elevated CO2 and temperature. In the present project, the investigators will conduct both short and prolonged exposure experiments at Palmer Station, Antarctica to determine the responses of Euphausia superba to elevated CO2 and temperature. The investigators will test hypotheses related to acid-base compensation and acclimation of various life stages of krill to elevated CO2 and temperature. Furthermore, they will determine these impacts on feeding, respiration, metabolism, growth, and reproduction.</p>
<p>The Antarctic krill, Euphausia superba, is a key species in Antarctic food webs as they are a primary food source for many of the top predators in the Southern Ocean including baleen whales, seals, penguins, and other sea birds. This project will determine the responses of Antarctic krill exposed to elevated CO2 and temperature and whether or not krill have the capacity to fully compensate under future ocean conditions. The proposed field effort will be complemented by an extensive broader impact effort focused on bringing marine science to both rural and urban high school students in the Midwest (Kansas). The core educational objectives of this proposal are to 1) instruct students about potential careers in marine science, 2) engage students and promote their interest in the scientific process, critical thinking, and applications of science, mathematics, and technology, and 3) and increase student and teacher awareness and understanding of the oceans and global climate change, with special focus on the Western Antarctic Peninsula region. Finally, this project will engage undergraduate and graduate students in the production, analysis, presentation and publication of datasets.</p>
OA Krill
largerWorkCitation
project
eng; USA
biota
oceans
-64.0526
-64.0526
-64.7741
-64.7741
2014-01-24
2014-02-24
Palmer Station, Antarctica
0
BCO-DMO catalogue of parameters from Data from two Antarctic krill (Euphausia superba) 24-hour feeding experiments under ambient temperature and pCO2, ambient temperature and elevated pCO2, and elevated temperature and pCO2.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/820696.rdf
Name: Feeding_Experiment
Units: unitless
Description: <p>Experiment identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/820697.rdf
Name: Expt_Start_Date
Units: unitless
Description: <p>Experiment start date; formatted as ISO yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/820698.rdf
Name: Bottle_description
Units: unitless
Description: <p>Designation of control bottle (no krill; seawater only) and krill bottle (4 L bottle filled with treatment seawater plus one krill)</p>
http://lod.bco-dmo.org/id/dataset-parameter/820699.rdf
Name: Time
Units: unitless
Description: <p>Designation of sample collected at experiment start (T0) or experiment end (Tfinal)</p>
http://lod.bco-dmo.org/id/dataset-parameter/820700.rdf
Name: Treatment_temp
Units: unitless
Description: <p>Temperature treatment; either Ambient (0 degrees C) or Elevated (3 degrees C)</p>
http://lod.bco-dmo.org/id/dataset-parameter/820701.rdf
Name: Treatment_pCO2
Units: unitless
Description: <p>pCO2 treatment; either Ambient or Elevated (target = 800 ppm)</p>
http://lod.bco-dmo.org/id/dataset-parameter/820702.rdf
Name: Incubation_time
Units: hours
Description: <p>Length of time between start (T0) and end (Tfinal) of feeding experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/820703.rdf
Name: spec_pH
Units: unitless (pH units)
Description: <p>pH determined spectrophotometrically from subsamples of seawater in each experimental bottle during the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/820704.rdf
Name: Total_akalinity
Units: micromol/kilogram (umol/kg)
Description: <p>Total alkalinity determined from subsamples of seawater in each experimental bottle during the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/820705.rdf
Name: Chlorophyll_a
Units: micrograms/liter (ug/L)
Description: <p>Concentration of chlorophyll a determined fluorometrically from subsamples of filtered seawater in each experimental bottle during the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/820706.rdf
Name: Notes
Units: unitless
Description: <p>Notes pertaining to experiment</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5941
https://darchive.mblwhoilibrary.org/bitstream/1912/26110/1/dataset-820557_2014-antarctic-krill-feeding-experiments__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.820557.1
download
onLine
dataset
<p><strong>Sampling and analytical procedures:&nbsp;</strong></p>
<p><strong>Capture and husbandry:</strong> Antarctic krill (Euphausia superba) were captured during the austral summer of 2013/2014. Krill were collected by net tow (2 m diameter, 1000 m mesh, non-filtering cod end) off the R/V Laurence M. Gould near the Western Antarctic Peninsula and transported directly to the Palmer Station biological laboratory. One to two thousand krill were housed in one 4'w x 3'h circular holding tank and two 5’ x 2’ x 1’ rectangular tanks provided with aeration and flow-through seawater. Water was non-filtered and individuals were able to feed on plankton ad libitum throughout the season.</p>
<p><strong>Experimental treatments:</strong> Three experimental treatments were targeted in this study: (1) ambient temperature and ambient pCO2/pH (400ppm/8.10), (2) ambient temperature and elevated pCO2 (800ppm)/reduced pH (7.7), and (3) elevated temperature (3 degrees C) and elevated pCO2 (800ppm)/reduced pH (7.7). Two replicate feeding experiments were conducted: Experiment 1 and 2. Temperature treatments were obtained using two separate recirculating systems. Two 800 L cylindrical polycarbonate carboys were attached to temperature controlled chillers (Delta Star) and inline pumps. The carboys were filled with non-filtered seawater acquired from the Palmer Station intake line, placed in a flow-through water bath, and maintained at 0&nbsp;degrees C. Another 800 L carboy was set up without a chiller and placed in an environmental chamber set at bated for about 24 hours before sacrificed for sampling the end points. The samples collected in the bottles at T0 and Tfinal include: pH, total alkalinity, and fluorometric chlorophyll a.&nbsp;</p>
<p>The systems were replaced with new water daily and allowed to acclimate to temperature for a minimum of 24 hours before the start of a trial or water change. High CO2 conditions were obtained using a peristaltic pump to inject straight CO2 into the propeller of a pump submerged in seawater. Treated water was then gently siphoned with minimal disturbance into treatment bottles.&nbsp;</p>
<p>For each of the three treatments, 14, 4 L wide-mouth polycarbonate bottles were filled with the appropriate equilibrated seawater. Two bottles per treatment served as T0 controls (no krill added) and were sacrificed for an initial suite of samples. Two bottles served as Tfinal (24 h) controls, and one juvenile krill was added to each of the remaining 10 bottles per treatment (Tfinal treatments). The Tfinal bottles were capped to maintain target pCO2/pH, incubated in the appropriate location to maintain desired temperature (water bath for ambient, 0&nbsp;degrees C; 3 degrees C environmental chamber for elevated temperature), and incubated for about 24 hours before sacrificed for sampling the end points. The samples collected in the bottles at T0 and Tfinal include: pH, total alkalinity, and fluorometric chlorophyll a.</p>
<p><strong>Analyses:</strong>&nbsp; pH was determined spectrophotometrically using the indicator dye thymol blue (Dickson et al. 2007; Zhang and Byrne 1996). Total alkalinity was determined on 100 ml subsamples with an open-cell, potentiometric titration of seawater (Metrohm 888 Titrando) with 0.1 M HCl following the potential of a pH electrode (Dickson et al. 2007). Tiamo software (version 2.3) was used to process the alkalinity data. Measurements of pH and TA were quality controlled using certified reference materials (CRMs) obtained from Andrew Dickson at UCSD Scripps Institute of Oceanography. An aliquot of seawater from each incubation bottle was also filtered onto a GF/F filter, which was wrapped in foil and frozen for fluorometric chlorophyll a&nbsp; analysis (Parsons et al. 1984).&nbsp;Salinity was measured with a bench top conductivity meter (YSI 3100) calibrated daily with a conductivity standard (50,000 uS/cm; Ricca Chemical Company).</p>
<table border="0" cellpadding="0" cellspacing="0" style="width:515px">
<tbody>
<tr>
<td style="height:21px; width:312px"><strong>Expt 1</strong></td>
<td style="width:203px"><strong>Expt 2</strong></td>
</tr>
<tr>
<td style="height:21px">Salinity (ave +/- sterr; n = 30) = 32.43 +/- 0.02</td>
<td>Salinity (ave +/- sterr; n = 30) = 32.63 +/- 0.03</td>
</tr>
<tr>
<td style="height:21px">Ambient temperature = 0 C</td>
<td>Ambient temperature = 0 C</td>
</tr>
<tr>
<td style="height:21px">Elevated Temperature = 3 C</td>
<td>Elevated Temperature = 3 C</td>
</tr>
</tbody>
</table>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- data submitted in Excel file "2014_AntarcticKrill_GrowthExperiment.xlsx" sheet "Sheet1" extracted to csv<br />
-&nbsp;removed carbonate table to server separately<br />
-&nbsp;added conventional header with dataset name, PI name, version date<br />
-&nbsp;modified parameter names to conform with BCO-DMO naming conventions<br />
- converted dates to ISO format (yyyy-mm-dd)<br />
- replaced ND with nd for 'no data'</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Delta Star chiller
Delta Star chiller
PI Supplied Instrument Name: Delta Star chiller PI Supplied Instrument Description:Used to cool water to ambient temperature. Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.
YSI 3100 Conductivity Instrument
YSI 3100 Conductivity Instrument
PI Supplied Instrument Name: YSI 3100 Conductivity Instrument PI Supplied Instrument Description:Used to measure salinity in seawater. Instrument Name: Conductivity Meter Instrument Short Name:Conductivity Meter Instrument Description: Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.
PI Supplied Instrument Name: PI Supplied Instrument Description:Net with 2 m diameter, 1000 m mesh, non-filtering cod end. Used to collected krill for experimental analyses. Instrument Name: Plankton Net Instrument Short Name:Plankton Net Instrument Description: A Plankton Net is a generic term for a sampling net that is used to collect plankton. It is used only when detailed instrument documentation is not available. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/22/
Shimadzu spectrophotometer
Shimadzu spectrophotometer
PI Supplied Instrument Name: Shimadzu spectrophotometer PI Supplied Instrument Description:Used to measure pH in seawater. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Metrohm 888 Titrando
Metrohm 888 Titrando
PI Supplied Instrument Name: Metrohm 888 Titrando PI Supplied Instrument Description:Instrument used for open-cell titrations to determine total alkalinity in seawater. Used to measure total alkalinity in seawater. Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Turner 10 AU Fluorometer
Turner 10 AU Fluorometer
PI Supplied Instrument Name: Turner 10 AU Fluorometer PI Supplied Instrument Description:Used to measure chlorophyll a biomass as a proxy for phytoplankton (krill food) biomass. Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/