http://lod.bco-dmo.org/id/dataset/820948
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chlorophyll a concentrations measured along the cruise track of AE1812 which transected from the Sargasso Sea to Coastal Rhode Island during May 2018.
2025-08-13
publication
2025-08-13
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2025-08-13
publication
https://doi.org/10.26008/1912/bco-dmo.820948.2
Tatiana A. Rynearson
University of Rhode Island
principalInvestigator
Bethany D. Jenkins
University of Rhode Island
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Rynearson, T., Jenkins, B. (2020) Chlorophyll a concentrations measured along the cruise track of AE1812 which transected from the Sargasso Sea to Coastal Rhode Island during May 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.820948.1 [access date]
Methods and Sampling: <p>In situ Chlorophyll a was measured at each station along the AE1812 cruise track. A CTD was deployed to collect water from the euphotic zone. Samples were filtered in triplicate onto GF/F and 5µm and 20µm polycarbonate filters.</p>
<p>Chl a was extracted from filters in 100% denatured ethanol for 12 hours, measured on a 10-AU Fluorometer, and chlorophyll a and phaeophytin concentrations were calculated based on a predetermined calibration curve. Concentrations on each filter were used to determine total chl a within each size fraction.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1558490 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1558490
completed
Tatiana A. Rynearson
University of Rhode Island
401-874-6022
Graduate School of Oceanography, University of Rhode Island 215 South Ferry Rd.
Narragansett
RI
02882
USA
rynearson@uri.edu
pointOfContact
Bethany D. Jenkins
University of Rhode Island
401-874-7551
Department of Cell and Molecular Biology and Graduate School of Oceanography 279 CBLS, 120 Flagg Road
Kingston
RI
02881
USA
bdjenkins@uri.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
ISO_DateTime_UTC
lat_converted
lon_converted
station
cast
niskin_bottle_ID
depth
size_fraction
tube_ID
vol_filtered
vol_extracted
Fb
Fa
blank_subt_Fb
blank_subt_Fa
chla
phaeo
10AU Fluorometer (Turner Designs, San Jose, CA)
theme
None, User defined
ISO_DateTime_UTC
latitude
longitude
station
cast
Niskin bottle number
depth
No BCO-DMO term
sample identification
volume of water filtered
sample_volume
fluorescence
instrument_blank
chlorophyll a
total phaeopigment
featureType
BCO-DMO Standard Parameters
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
AE1812
service
Deployment Activity
Bermuda to Rhode Island
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic
https://osprey.bco-dmo.org/project/704768
Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic
<p>NSF abstract:</p>
<p>About half of photosynthesis on earth is generated by marine phytoplankton, single celled organisms that drift with tides and currents. Within the phytoplankton, the diatoms conduct nearly half of this photosynthesis, exerting profound control over global carbon cycling. Despite their importance, there are surprisingly fundamental gaps in understanding how diatoms function in their natural environment, in part because methods to assess in situ physiology are lacking. This project focuses on the application of a powerful new approach, called Quantitative Metabolic Fingerprinting (QMF), to address this knowledge gap and examine species-specific physiology in the field. The project will provide transformative insights into how ocean geochemistry controls the distribution of diatoms, the metabolic responses of individual diatom species, and how metabolic potential is partitioned between diatom species, thus providing new insights into the structure and function of marine systems. The overarching goal is to examine how diatom species respond to changes in biogeochemistry across marine provinces, from the coast to the open ocean, by following shifts in diatom physiology using QMF. This research is critical to understand future changes in oceanic phytoplankton in response to climate and environmental change. Furthermore, activities on this project will include supporting a graduate student and postdoctoral fellow and delivering the Artistic Oceanographer Program (AOP) to diverse middle school age children and teachers in the NYC metropolitan area and to middle-school girls in the Girl Scouts of RI, reaching an anticipated 60 children and 30 teachers annually. The programs will foster multidisciplinary hands-on learning and will directly impact STEM education at a critical point in the pipeline by targeting diverse middle-school aged groups in both NY and RI.</p>
<p>In laboratory studies with cultured isolates, there are profound differences among diatom species' responses to nutrient limitation. Thus, it is likely that different species contribute differently to nutrient uptake, carbon flux and burial. However, marine ecosystem models often rely on physiological attributes drawn from just one species and apply those attributes globally (e.g. coastal species used to model open ocean dynamics) or choose a single average value to represent all species across the world's oceans. In part, this is due to a relatively poor understanding of diatom physiological ecology and a limited tool set for assessing in situ diatom physiological ecology. This research project will address this specific challenge by explicitly tracking metabolic pathways, measuring their regulation and determining their taxonomic distribution in a suite of environmentally significant diatoms using a state of the art, species-specific approach. A research expedition is set in the North Atlantic, a system that plays a major role in carbon cycling. Starting with a New England coastal shelf site, samples will be collected from the coast where diatoms thrive, to the open ocean and a site of a long term ocean time series station (the Bermuda Atlantic Time Series) where diatom growth is muted by nutrient limitation. This research takes advantage of new ocean observatories initiative (OOI) and time series information. Through the research expedition and downstream laboratory experiments, the molecular pathways of nutrient metabolism and related gene expression in a suite of environmentally significant diatoms will be identified. Data will be combined to predict major limiting factors and potentially important substrates for diatoms across marine provinces. Importantly, this integrated approach takes advantage of new advances in molecular and bioinformatics tools to examine in situ physiological ecology at the species-specific level, a key knowledge gap in the field.</p>
North Atlantic Diatoms
largerWorkCitation
project
eng; USA
oceans
Bermuda to Rhode Island
-70.96835
-63.2384
31.684933
41.193917
2018-05-02
2018-05-15
North Atlantic
0
BCO-DMO catalogue of parameters from Chlorophyll a concentrations measured along the cruise track of AE1812 which transected from the Sargasso Sea to Coastal Rhode Island during May 2018.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/821364.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/821365.rdf
Name: lat_converted
Units: degrees
Description: <p>Latitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/821366.rdf
Name: lon_converted
Units: degrees
Description: <p>Longitude of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/821367.rdf
Name: station
Units: unitless
Description: <p>Station of CTD cast</p>
http://lod.bco-dmo.org/id/dataset-parameter/821368.rdf
Name: cast
Units: unitless
Description: <p>CTD cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/821369.rdf
Name: niskin_bottle_ID
Units: unitless
Description: <p>Bottle ID of niskin used to collect sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/821370.rdf
Name: depth
Units: meters (m)
Description: <p>depth of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/821371.rdf
Name: size_fraction
Units: micrometers (um)
Description: <p>size fraction analyzed based on pore size of filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/821372.rdf
Name: tube_ID
Units: unitless
Description: <p>ID of the tube</p>
http://lod.bco-dmo.org/id/dataset-parameter/821373.rdf
Name: vol_filtered
Units: milliliters (mL)
Description: <p>Volume of seawater filtered for chl a analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/821374.rdf
Name: vol_extracted
Units: milliliters (mL)
Description: <p>Volume of ethanol used to extract chl a</p>
http://lod.bco-dmo.org/id/dataset-parameter/821375.rdf
Name: Fb
Units: Relative Fluorescence Units (RFU)
Description: <p>Fluorescence before acidification</p>
http://lod.bco-dmo.org/id/dataset-parameter/821376.rdf
Name: Fa
Units: Relative Fluorescence Units (RFU)
Description: <p>Fluorescence after acidification</p>
http://lod.bco-dmo.org/id/dataset-parameter/821377.rdf
Name: blank_subt_Fb
Units: Relative Fluorescence Units (RFU)
Description: <p>Blank corrected fluorescence before acidification</p>
http://lod.bco-dmo.org/id/dataset-parameter/821378.rdf
Name: blank_subt_Fa
Units: Relative Fluorescence Units (RFU)
Description: <p>Blank corrected fluorescence after acidification</p>
http://lod.bco-dmo.org/id/dataset-parameter/821379.rdf
Name: chla
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyll a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/821380.rdf
Name: phaeo
Units: micrograms per liter (ug/L)
Description: <p>Phaeophytin concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
64304
https://darchive.mblwhoilibrary.org/bitstream/1912/26125/1/dataset-820948_ae1812-situ-chl__v1.tsv
download
64304
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/b5223a6e-7c6a-4b8c-9672-62ad78c5db7b/content
download
https://doi.org/10.26008/1912/bco-dmo.820948.2
download
onLine
dataset
<p>In situ Chlorophyll a was measured at each station along the AE1812 cruise track. A CTD was deployed to collect water from the euphotic zone. Samples were filtered in triplicate onto GF/F and 5µm and 20µm polycarbonate filters.</p>
<p>Chl a was extracted from filters in 100% denatured ethanol for 12 hours, measured on a 10-AU Fluorometer, and chlorophyll a and phaeophytin concentrations were calculated based on a predetermined calibration curve. Concentrations on each filter were used to determine total chl a within each size fraction.</p>
Specified by the Principal Investigator(s)
<p>Data was processed in R 3.6.2 (R-Core-Team 2019)</p>
Specified by the Principal Investigator(s)
Version 1 (chla_insitu.csv)
* Added a conventional header with dataset name, PI name, version date.
* Modified parameter names to conform with BCO-DMO naming conventions.
* Blank values in this dataset are displayed as "nd" for "no data." nd is the default missing data identifier in the BCO-DMO system.
* Converted datetime into ISO_DateTime_UTC format.
* Converted latitude and longitude from degrees decimal minutes to decimal degrees.
* Set types for each data column.
Version 2 (chla_insitu.csv)
* Metadata corrections and enhancements were made to parameter units. Parameter units have been fully typed to include long form names, grams per liter (g/L) units have been corrected to micrograms per liter (ug/L), and the size fraction units have been corrected from m to micrometer (um).
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10AU Fluorometer (Turner Designs, San Jose, CA)
10AU Fluorometer (Turner Designs, San Jose, CA)
PI Supplied Instrument Name: 10AU Fluorometer (Turner Designs, San Jose, CA) Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Cruise: AE1812
AE1812
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE1812
Tatiana A. Rynearson
University of Rhode Island
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel