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            <gco:CharacterString>Cite this dataset as: Kimbro, D. L., Stallings, C. D., White, J. W. (2020) Surveys of inhabitants on the intertidal and subtidal oyster reef in Apalachicola Bay and Ocholckonee Bay, FL, 2013-2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-07-29 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.821071.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Survey totals Dataset Description: &amp;lt;p&amp;gt;Surveys of inhabitants on the intertidal and subtidal oyster reef in Apalachicola Bay and Ocholckonee Bay, Florida, 2013-2019. This data includes the total numbers of adults, spat, live oysters, new and old gapers, and whether mussels,&amp;amp;nbsp;barnacles crabs, or gastropods were present.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Site Selection- From Hanley et. al 2019, this was a multi-step process that first involved using ArcGIS to partition the bay’s oyster reefs (commercial and non-commercial) into six zones. Zone assignment was based on the reef’s relative distance by water from the river input (near, mid, far) as well as a reef’s east-west orientation to the river (East Apalachicola and West Apalachicola). Next, we randomly selected three reefs out of all possible reefs (including the experimental reefs) within each zone.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Surveys- Methods for surveys done from 2013-2016 were taken from Hanley et al. 2019.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Surveys 2013-2016-On each reef subtidal reef, we obtained spatially balanced samples by extending four 20 m transects at 90 degree angles from the boat. Along each transect, we overlaid a 0.25 m2&amp;amp;nbsp;weighted quadrat at the 5, 10, 15, and 20 m marks. For each quadrat, we collected the entire contents of the quadrat into a uniquely labeled mesh bag, transported the bag to the surface, and placed the bag on ice to be processed at the lab. For intertidal reefs, we sampled 2 quadrats per reef, ‘low’ (located at the low water level) and ‘high’ (2 m above the low transect) quadrats centered along a 20 m transect on each reef.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Surveys 2019- Sites were selected from previously sampled reefs. Subtidal surveys were conducted by divers on SCUBA. A buoy was dropped at the sites. The divers attached a 10m transect to the buoy and surveyed the area in the four cardinal directions (North, South, East and West). We characterized the substrate 0.5m on each side of the transect as mud, sand or shell hash, as well as quantified the amount of oyster shells and counted the number of Oyster Drills (Stramonita haemastoma). If there were oysters or shells at meter 5 or meter 10 along the transect then everything in a 0.25m2 quadrat was collected and processed at the lab. We only sampled the intertidal sites in December 2019. We laid a transect across the crest of the reef and then divided the length of the transect by 7 to get a consistent sampling interval. At each sampling interval we recorded the width of the sampling side and then placed a 0.0625m2 quadrat at the midpoint of the sampling width and collected all the contents to be brought back to the lab and processed.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/816536.rdf" xlink:title="OCE-1917015" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1917015 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1917015</gmx:Anchor>
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&lt;p&gt;Ecosystems can exhibit &quot;tipping points&quot; whereby an environmental disturbance pushes an ecosystem into an altered state from which it does not recover, even when the environment normalizes. This may have happened to valuable oyster reefs in Northwest Florida in 2012, when drought and low river flow allowed predators of oysters to flourish and consume nearly all the oysters. Despite subsequent years of normal rainfall and river flow, oysters have not recovered, suggesting the ecosystem may have crossed a tipping point. However, the timing and magnitude of the disturbance from Hurricane Michael (2018) may have pushed the ecosystem back towards its original, healthy state. In this project, investigators make field observations to gauge how predators and oysters are responding to Hurricane Michael and conduct lab experiments to test how predators and oysters respond to hurricane rainfall conditions. Additionally, they use mathematical models to predict whether effects observed in the field and lab could lead to a shift back past the tipping point. This is a rare opportunity to study how oyster ecosystems can shift back from altered to healthy states. However, a rapid response is essential before seasonal changes in the weather and bay obscure hurricane impacts. This research has several broader impacts. First, it will expand the ecological theory of tipping points. Second, it can support the management of the Apalachicola Bay oyster fishery, such as insight into the likely success of restoration efforts. The team coordinates with the Apalachicola National Estuarine Research Reserve to this end. Finally, research outputs are incorporated into ongoing public education and training efforts.&lt;/p&gt;
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Ecosystems can rapidly shift from their original, high-value state to a new, degraded one. Such shifts have been observed in many ecosystems, but it is sometimes difficult to identify the mechanisms that mediate the shift beyond a &quot;tipping point&quot; and - to a greater extent - those that could mediate a shift back to the original state. Improving our understanding and predictive capability of tipping points depends on identifying the mechanisms that underlie bi-directional system shifts. In 2012, the oyster reefs of Apalachicola Bay, FL abruptly shifted into an oyster-less state when prolonged drought and low river flow allowed marine oyster predators to flourish. Despite subsequent years of normal rainfall and flow, there has not been a return shift, suggesting this ecosystem may have entered an alternate stable state. The hypothesis of this work is that in 2018 Hurricane Michael provided a sufficient disturbance to shift the system back into the attracting basin for its original state (prior observations support this prediction). This project couples field observations and lab experiments with population modeling to test whether and how Hurricane Michael initiated a reversal shift. A rapid response is essential before seasonal variability in this ecosystem obscures hurricane effects. The proposal's intellectual merit is based on its ability to address a central goal in ecology: identifying and predicting ecosystem tipping points. Combining empirical observations and models is a promising approach to advance this goal, but has not been widely applied in the field, mainly because researchers are not in place at the time of a shift. Hurricane Michael provides a unique opportunity to address this knowledge gap.&lt;/p&gt;
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            <gco:CharacterString>BCO-DMO catalogue of parameters from Surveys of inhabitants on the intertidal and subtidal oyster reef in Apalachicola Bay and Ocholckonee Bay, FL, 2013-2019</gco:CharacterString>
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            http://lod.bco-dmo.org/id/dataset-parameter/825431.rdf
	Name: reef_type
	Units: unitless
	Description: &lt;p&gt;whether reef is subtidal or intertidal&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825432.rdf
	Name: estuary
	Units: unitless
	Description: &lt;p&gt;name of the estuary: Apalachicola or Ocholckonee&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825433.rdf
	Name: region
	Units: unitless
	Description: &lt;p&gt;region: east/west/Ochlockonee&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825434.rdf
	Name: distance
	Units: unitless
	Description: &lt;p&gt;distance from the river: zone 1 or 2 or 3. 1 is the closest and 3 is the furthest from freshwater input&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825435.rdf
	Name: harvest_date
	Units: unitless
	Description: &lt;p&gt;date sample was collected; formatted as yyyy-mm-dd&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825436.rdf
	Name: reef_name
	Units: unitless
	Description: &lt;p&gt;name of reef&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825437.rdf
	Name: reef
	Units: unitless
	Description: &lt;p&gt;reef identifier within reef_name categories&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825438.rdf
	Name: transect
	Units: unitless
	Description: &lt;p&gt;transect identifier: high/low for intertidal; C1/C2/S1/S2 for old reef sampling; N/E/S/W for 2015 sampling; 0/130/240 for 2016 sampling&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825439.rdf
	Name: quadrat
	Units: unitless
	Description: &lt;p&gt;meter mark of the quadrat; refers to the location along a 20 meter transect at which point the Quadrat was deployed and the sample was taken. Usually quadrats were deployed at 5 meter; 10 meter; 15 meter; and 20 meter marks on the transect tape.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825440.rdf
	Name: latitude
	Units: decimal degrees
	Description: &lt;p&gt;latitude; north is positive&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825441.rdf
	Name: longitude
	Units: decimal degrees
	Description: &lt;p&gt;longitude; east is positive&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825442.rdf
	Name: process_date
	Units: unitless
	Description: &lt;p&gt;date sample was processed; formatted as yyyy-mm-dd&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825443.rdf
	Name: days_til_process
	Units: days
	Description: &lt;p&gt;days between collection and processing&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825444.rdf
	Name: processed
	Units: unitless
	Description: &lt;p&gt;whether the collected sample was processed or not (yes/no)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825445.rdf
	Name: wt_samp_kg
	Units: kilograms
	Description: &lt;p&gt;sample weight&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825446.rdf
	Name: total_adults
	Units: oysters
	Description: &lt;p&gt;total number of adults. Oysters 25mm or greater&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825447.rdf
	Name: total_spat
	Units: oysters
	Description: &lt;p&gt;total number of spat. Oysters less than 25mm&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825448.rdf
	Name: total_live
	Units: oysters
	Description: &lt;p&gt;total number of live oysters&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825449.rdf
	Name: total_newg
	Units: oysters
	Description: &lt;p&gt;total number of new gapers&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825450.rdf
	Name: total_oldg
	Units: oysters
	Description: &lt;p&gt;total number of old gapers&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825451.rdf
	Name: muss_present
	Units: unitless
	Description: &lt;p&gt;whether mussels were present: yes/no&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825452.rdf
	Name: barn_present
	Units: unitless
	Description: &lt;p&gt;whether barnacles were present: yes/no&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825453.rdf
	Name: transect_stone_crab_burrows
	Units: burrows
	Description: &lt;p&gt;number of stone crabs counted along the transect; only one observation per transect (not per quadrat) so value was assigned to quadrat 5 for each transect for all subtidal reefs; and the first quadrat meter mark for low &amp;amp; high transects at intertidal reefs&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825454.rdf
	Name: transect_blue_crab
	Units: blue crabs
	Description: &lt;p&gt;number of blue crabs counted along the transect; only one observation per transect (not per quadrat) so value was assigned to quadrat 5 for each transect for all subtidal reefs; and the first quadrat meter mark for low &amp;amp; high transects at intertidal reefs&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825455.rdf
	Name: transect_drills
	Units: oyster drills
	Description: &lt;p&gt;number of oyster drills counted along the transect; only one observation per transect (not per quadrat) so value was assigned to quadrat 5 for each transect for all subtidal reefs; and the first quadrat meter mark for low &amp;amp; high transects at intertidal reefs&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825456.rdf
	Name: transect_conch
	Units: conch
	Description: &lt;p&gt;number of conch counted along the transect; only one observation per transect (not per quadrat) so value was assigned to quadrat 5 for each transect for all subtidal reefs; and the first quadrat meter mark for low &amp;amp; high transects at intertidal reefs&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825457.rdf
	Name: transect_gastropod
	Units: gastropods
	Description: &lt;p&gt;conch and drills along the transect were identified in 2016 only. So for 2016; transect_gastropod is the sum of transect_drills and transect_conch; for all other years transect_gastropod is the count of all gastropods observed on the transect (species not id&amp;#039;d or limited to drill and conch)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825458.rdf
	Name: visibility_m
	Units: meters
	Description: &lt;p&gt;distance visible along underwater transect&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825459.rdf
	Name: notes
	Units: unitless
	Description: &lt;p&gt;notes from a previous data file&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825460.rdf
	Name: pred_notes
	Units: unitless
	Description: &lt;p&gt;if there were oddities in the predator data; a note was made here&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825461.rdf
	Name: lat_long_notes
	Units: unitless
	Description: &lt;p&gt;notes about discrepancies or oddities that were found in the data and a decision about which lat/long to use&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/825462.rdf
	Name: data_notes
	Units: unitless
	Description: &lt;p&gt;if there were oddities in the data; a note was made here&lt;/p&gt; 
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                <gco:CharacterString>&amp;lt;p&amp;gt;Site Selection- From Hanley et. al 2019, this was a multi-step process that first involved using ArcGIS to partition the bay’s oyster reefs (commercial and non-commercial) into six zones. Zone assignment was based on the reef’s relative distance by water from the river input (near, mid, far) as well as a reef’s east-west orientation to the river (East Apalachicola and West Apalachicola). Next, we randomly selected three reefs out of all possible reefs (including the experimental reefs) within each zone.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Surveys- Methods for surveys done from 2013-2016 were taken from Hanley et al. 2019.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Surveys 2013-2016-On each reef subtidal reef, we obtained spatially balanced samples by extending four 20 m transects at 90 degree angles from the boat. Along each transect, we overlaid a 0.25 m2&amp;amp;nbsp;weighted quadrat at the 5, 10, 15, and 20 m marks. For each quadrat, we collected the entire contents of the quadrat into a uniquely labeled mesh bag, transported the bag to the surface, and placed the bag on ice to be processed at the lab. For intertidal reefs, we sampled 2 quadrats per reef, ‘low’ (located at the low water level) and ‘high’ (2 m above the low transect) quadrats centered along a 20 m transect on each reef.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Surveys 2019- Sites were selected from previously sampled reefs. Subtidal surveys were conducted by divers on SCUBA. A buoy was dropped at the sites. The divers attached a 10m transect to the buoy and surveyed the area in the four cardinal directions (North, South, East and West). We characterized the substrate 0.5m on each side of the transect as mud, sand or shell hash, as well as quantified the amount of oyster shells and counted the number of Oyster Drills (Stramonita haemastoma). If there were oysters or shells at meter 5 or meter 10 along the transect then everything in a 0.25m2 quadrat was collected and processed at the lab. We only sampled the intertidal sites in December 2019. We laid a transect across the crest of the reef and then divided the length of the transect by 7 to get a consistent sampling interval. At each sampling interval we recorded the width of the sampling side and then placed a 0.0625m2 quadrat at the midpoint of the sampling width and collected all the contents to be brought back to the lab and processed.&amp;lt;/p&amp;gt;</gco:CharacterString>
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- re-ordered columns&amp;lt;br /&amp;gt;
- sorted rows by&amp;amp;nbsp;{reef_type}{estuary}{region}{distance}{harvest_date}{reef_name}{reef}{transect}{quadrat}&amp;lt;/p&amp;gt;

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				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
				  </gmd:postalCode>
				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
		</gmd:CI_Contact>
  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact"  codeSpace="007">pointOfContact</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation>
    <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/616065.rdf" xlink:title="CTD Sea-Bird SBE SEACAT 19plus" xlink:actuate="onRequest">Seabird SBE 19plus V2</gmx:Anchor>
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          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Seabird SBE 19plus V2</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Seabird SBE 19plus V2 PI Supplied Instrument Description:Used to measure conductivity, temperature, and pressure and with auxiliary sensors, measures dissolved oxygen, pH, turbidity, fluorescence, oil, PAR, nitrates, altimeter, etc. Instrument Name: CTD Sea-Bird SBE SEACAT 19plus Instrument Short Name:Sea-Bird SEACAT 19 Plus   Instrument Description: Self contained self powered CTD profiler. Measures conductivity, temperature and pressure in both profiling (samples at 4 scans/sec) and moored (sample rates of once every 5 seconds to once every 9 hours) mode. Available in plastic or titanium housing with depth ranges of 600m and 7000m respectively. Minature submersible pump provides water to conductivity cell.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/678.rdf" xlink:title="Water Quality Multiprobe" xlink:actuate="onRequest">YSI Pro 2030</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>YSI Pro 2030</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: YSI Pro 2030 PI Supplied Instrument Description:Used to measure dissolved oxygen, temperature, salinity and pH. Instrument Name: Water Quality Multiprobe Instrument Short Name:Water Quality Multiprobe   Instrument Description: An instrument which measures multiple water quality parameters based on the sensor configuration.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
