http://lod.bco-dmo.org/id/dataset/821587
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-20
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019
2023-07-23
publication
2023-07-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-03-17
publication
https://doi.org/10.26008/1912/bco-dmo.821587.2
Samantha B. Joye
University of Georgia
principalInvestigator
Andreas Teske
University of North Carolina at Chapel Hill
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Joye, S. B., Teske, A. (2023) Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2023-07-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.821587.2 [access date]
Methods and Sampling: <p>Sampling and analytical procedures:</p>
<p>CH4:<br />
&nbsp;Samples were collected by filling 160 ml glass serum vials with seawater, stoppering them headspace-free with butyl chloride stoppers, and crimp sealing them. Samples were killed by injecting 0.5 ml 8 M NaOH. Ten ml killed sample was then displaced with a ten ml UHP nitrogen gas headspace. Samples were stored at room temperature until analysis. At the time of analysis, an additional ten ml of nitrogen was added to the vials. Vials were shaken for 2 min before headspace was sampled and injected into a GC FID (Shimadzu GC 2014, Alltech Carbosphere 80/100 6’x1/8” column). This method is adapted from Magen, 2014 (A simple headspace equilibration method for measuring dissolved methane. Limnol. Oceanogr. Methods 12(9): 637-650).&nbsp;&nbsp;<br />
<br />
MOx [data in both cruises]:<br />
Four technical replicates were collected from each sampled depth by filling 16.6 mL Hungate&nbsp;tubes headspace-free and sealing with exetainer septa. Tubes were stored at 4 C until injection.&nbsp;One of the four replicates&nbsp;was killed by displacing 1.6 ml sample with 1.6 ml 37% formalin. All samples, including the killed abiotic control, were injected with ~2000000 dpm tritiated methane tracer. Samples were incubated at near-in situ temperature for 1-4 days. Immediately before incubation termination, a 100 ul subsample was removed from each tube and measured on a liquid scintillation counter to determine total dissolved 3H-methane addition. Incubations were terminated by killing with 1.6 ml 37% formalin and purging with air for 1 hour to remove remaining tracer. A 5 ml aliquot of each killed replicate was measured on a liquid scintillation counter to determine the ratio of tracer turnover. The turnover ratio was multiplied by the measured in situ methane concentration to obtain the MOx rate. Killed controls were subtracted and the replicates averaged to obtain the final rate number. See Rogener, 2018 (Long-term impact of the Deepwater Horizon oil well blowout on methane oxidation dynamics in the northern Gulf of Mexico. Elem Sci Anth, 6(1), 73).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357360 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357360
completed
Samantha B. Joye
University of Georgia
706-542-5893
Dept. of Marine Sciences 325 Sanford Drive
Athens
GA
30602
USA
mjoye@uga.edu
pointOfContact
Andreas Teske
University of North Carolina at Chapel Hill
508-287-1648
Department of Marine Sciences 3117B Venable Hall, CB 3300
Chapel Hill
NC
27599
USA
teske@email.unc.edu
pointOfContact
asNeeded
Dataset Version: 2
Unknown
Cruise_id
Event
Deployment_Type
Site
Depth
Incubation_temp
CH4
MOx_rate_avg
MOx_rate_stddev
SRI Instruments 8610C Gas Chromatograph with FID Detector
Beckman-Coulter LS6500 liquid scintillation counter
Perkin Elmer TriCarb liquid scintillation counter
theme
None, User defined
Cruise identfier
event
event type
site
depth
water temperature at measurement depth
methane
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Gas Chromatograph
Liquid Scintillation Counter
Liquid Scintillation Counter
instrument
BCO-DMO Standard Instruments
AT42-05
FK190211
service
Deployment Activity
Guaymas Basin, Gulf of California, Mexico
Gulf of California
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Microbial Carbon cycling and its interactions with Sulfur and Nitrogen transformations in Guaymas Basin hydrothermal sediments
https://osprey.bco-dmo.org/project/474317
Collaborative Research: Microbial Carbon cycling and its interactions with Sulfur and Nitrogen transformations in Guaymas Basin hydrothermal sediments
<p><em>Description from NSF award abstract:</em><br />
Hydrothermally active sediments in the Guaymas Basin are dominated by novel microbial communities that catalyze important biogeochemical processes in these seafloor ecosystems. This project will investigate genomic potential, physiological capabilities and biogeochemical roles of key uncultured organisms from Guaymas sediments, especially the high-temperature anaerobic methane oxidizers that occur specifically in hydrothermally active sediments (ANME-1Guaymas). The study will focus on their role in carbon transformations, but also explore their potential involvement in sulfur and nitrogen transformations. First-order research topics include quantifying anaerobic methane oxidation under high temperature,in situ concentrations of phosphorus and methane , and with alternate electron acceptors; sulfate and sulfur-dependent microbial pathways and isotopic signatures under these conditions; and nitrogen transformations in methane-oxidizing microbial communities, hydrothermal mats and sediments.</p>
<p>This integrated biogeochemical and microbiological research will explore the pathways of and environmental controls on the consumption and production of methane, other alkanes, inorganic carbon, organic acids and organic matter that fuel the Guaymas sedimentary microbial ecosystem. The hydrothermal sediments of Guaymas Basin provide a spatially compact, high-activity location for investigating novel modes of methane cycling and carbon assimilation into microbial biomass. In the case of anaerobic methane oxidation, the high temperature and pressure tolerance of Guaymas Basin methane-oxidizing microbial communities, and their potential to uncouple from the dominant electron acceptor sulfate, vastly increase the predicted subsurface habitat space and biogeochemical role for anaerobic microbial methanotrophy in global deep subsurface diagenesis. Further, microbial methane production and oxidation interlocks with syulfur and nitrogen transformations, which will be explored at the organism and process level in hydrothermal sediment microbial communities and mats of Guaymas Basin. In general, first-order research tasks (rate measurements, radiotracer incorporation studies, genomes, in situ microgradients) define the key microbial capabilities, pathways and processes that mediate chemical exchange between the subsurface hydrothermal/seeps and deep ocean waters.</p>
Guaymas Basin Interactions
largerWorkCitation
project
eng; USA
oceans
Guaymas Basin, Gulf of California, Mexico; Gulf of California
-111
-111
27
27
2018-11-01
2019-03-01
Guaymas Basin, Gulf of California, 27.00 N, 111.00W
0
BCO-DMO catalogue of parameters from Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/821600.rdf
Name: Cruise_id
Units: unitless
Description: <p>Cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/821601.rdf
Name: Event
Units: unitless
Description: <p>Event or Deployment identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/821602.rdf
Name: Deployment_Type
Units: unitless
Description: <p>Deployment type</p>
http://lod.bco-dmo.org/id/dataset-parameter/821603.rdf
Name: Site
Units: unitless
Description: <p>Site name</p>
http://lod.bco-dmo.org/id/dataset-parameter/821604.rdf
Name: Depth
Units: meters (m)
Description: <p>Sample collection depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/821605.rdf
Name: Incubation_temp
Units: degrees Celsius
Description: <p>Radiotracer incubation temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/821606.rdf
Name: CH4
Units: nanomolar (nM)
Description: <p>Dissolved methane concentration, BDL=Below detection limit, method detection limit 1.0</p>
http://lod.bco-dmo.org/id/dataset-parameter/821607.rdf
Name: MOx_rate_avg
Units: picomoles per liter per day, pmol/L/d (pM/d)
Description: <p>Average methane oxidation rate, BDL=Below detection limit, method detection limit 1.0</p>
http://lod.bco-dmo.org/id/dataset-parameter/821608.rdf
Name: MOx_rate_stddev
Units: picomoles per liter per day, pmol/L/d (pM/d)
Description: <p>Standard deviation of MOx rate (n=3), BDL=Below detection limit, method detection limit 1.0</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10613
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/c80ac409-4936-4666-857f-99840db0505c/content
download
https://doi.org/10.26008/1912/bco-dmo.821587.2
download
onLine
dataset
<p>Sampling and analytical procedures:</p>
<p>CH4:<br />
&nbsp;Samples were collected by filling 160 ml glass serum vials with seawater, stoppering them headspace-free with butyl chloride stoppers, and crimp sealing them. Samples were killed by injecting 0.5 ml 8 M NaOH. Ten ml killed sample was then displaced with a ten ml UHP nitrogen gas headspace. Samples were stored at room temperature until analysis. At the time of analysis, an additional ten ml of nitrogen was added to the vials. Vials were shaken for 2 min before headspace was sampled and injected into a GC FID (Shimadzu GC 2014, Alltech Carbosphere 80/100 6’x1/8” column). This method is adapted from Magen, 2014 (A simple headspace equilibration method for measuring dissolved methane. Limnol. Oceanogr. Methods 12(9): 637-650).&nbsp;&nbsp;<br />
<br />
MOx [data in both cruises]:<br />
Four technical replicates were collected from each sampled depth by filling 16.6 mL Hungate&nbsp;tubes headspace-free and sealing with exetainer septa. Tubes were stored at 4 C until injection.&nbsp;One of the four replicates&nbsp;was killed by displacing 1.6 ml sample with 1.6 ml 37% formalin. All samples, including the killed abiotic control, were injected with ~2000000 dpm tritiated methane tracer. Samples were incubated at near-in situ temperature for 1-4 days. Immediately before incubation termination, a 100 ul subsample was removed from each tube and measured on a liquid scintillation counter to determine total dissolved 3H-methane addition. Incubations were terminated by killing with 1.6 ml 37% formalin and purging with air for 1 hour to remove remaining tracer. A 5 ml aliquot of each killed replicate was measured on a liquid scintillation counter to determine the ratio of tracer turnover. The turnover ratio was multiplied by the measured in situ methane concentration to obtain the MOx rate. Killed controls were subtracted and the replicates averaged to obtain the final rate number. See Rogener, 2018 (Long-term impact of the Deepwater Horizon oil well blowout on methane oxidation dynamics in the northern Gulf of Mexico. Elem Sci Anth, 6(1), 73).</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing Notes:<br />
- data submitted in Excel files "AT42-05_MOx_summary.xlsx"&nbsp; and "FK190211_MOx_summary.xlsx" extracted to csv and combined into one data table. [Column "Deployment Number" in FK190211 data added to "Event" column]&nbsp;<br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- blank cells originally submitted as 'ND' are displayed as nd, the default missing data identifier in the BCO-DMO data view.<br />
- version 2 replaces version 1 on 2023-07-23.&nbsp;CH4 data added for the FK190211 cruise</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
SRI Instruments 8610C Gas Chromatograph with FID Detector
SRI Instruments 8610C Gas Chromatograph with FID Detector
PI Supplied Instrument Name: SRI Instruments 8610C Gas Chromatograph with FID Detector Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
Beckman-Coulter LS6500 liquid scintillation counter
Beckman-Coulter LS6500 liquid scintillation counter
PI Supplied Instrument Name: Beckman-Coulter LS6500 liquid scintillation counter Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Perkin Elmer TriCarb liquid scintillation counter
Perkin Elmer TriCarb liquid scintillation counter
PI Supplied Instrument Name: Perkin Elmer TriCarb liquid scintillation counter Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Cruise: AT42-05
AT42-05
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT42-05
Andreas Teske
University of North Carolina at Chapel Hill
Cruise: FK190211
FK190211
R/V Falkor
Community Standard Description
International Council for the Exploration of the Sea
R/V Falkor
vessel
FK190211
Samantha B. Joye
University of Georgia
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
R/V Falkor
Community Standard Description
International Council for the Exploration of the Sea
R/V Falkor
vessel