http://lod.bco-dmo.org/id/dataset/821982
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Elemental ratios in Western Pacific sclerosponges
2020-08-26
publication
2020-08-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-08-31
publication
https://doi.org/10.26008/1912/bco-dmo.821982.1
Andréa G. Grottoli
Ohio State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grottoli, A. (2020) Elemental ratios in Western Pacific sclerosponges. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.821982.1 [access date]
Elemental ratios in Western Pacific sclerosponges Dataset Description: <p>A complete description of the collection and experimental methods is available in Grottoli et al. (2010). In brief, six <em>A. wellsi</em> sclerosponges were identified between 11.5 and 18 m depths along the wall at Short Drop Off, Palau. The specimens were stained with Alizarin Red on 26 July 2001, re-cemented onto the reef at a common depth of 11 m depth using Splash Zone marine epoxy, and allowed to grow out past the stain line for 2 years. In Saipan, three <em>A. wellsi</em> identified at 6 m depth and four <em>A. willeyana</em> sclerosponges identified between 7 and 9 m depth were stained with Alizarin Red on 15 July 2001, re-cemented onto the reef at a common depth of 8.3 m depth with marine epoxy, and allowed to grow out past the stain line for 2 years. On 15 July 2003 and 11 July 2003 all of the specimens were collected from Palau and Saipan, respectively, and were returned to the lab for further analysis.</p> Methods and Sampling: <p>In the laboratory, each specimen was cut down its major growth axis, cleaned with deionized water, and dried at 60°C for 3 days. The sclerosponge&nbsp;skeletons were then sampled in two ways for elemental analysis. First, bulk measurements spanning the entire two-year common time period established by the stain lines were obtained from each specimen. Each bulk skeletal sample was milled from the growing edge to the stain line using a Dremmel tool fitted with a diamond-tipped dental drill bit. Second, high-resolution samples were milled at 0.5 mm increments using a Merchantek Micromill from two <em>A. wellsi</em> specimens from Palau, one <em>A. wellsi</em>specimens from Saipan, and one A. willeyana specimen from Saipan from the growing edge to the stain line.</p>
<p><strong>Sample Solutions and Standards:</strong> All solutions were made with MilliQ water (18 MΩ; Millipore, MA) and ultrapure reagents unless otherwise noted. All labware was pre-cleaned with 5% v/v HNO₃, 20% v/v HCl and MilliQ water for a minimum of 10 hours each prior to sample handling in a Laminar Flow Exhausting Hood. Gravimetric standards were used to make calibration curves for P, Pb, Ba, and Ca. Stock standard solutions (CPI International) were diluted with ultrapure 2% v/v HNO₃ to concentrations that matched expected range of sample concentrations.</p>
<p><strong>Sample Preparation:</strong> Bulk sclerosponge samples of approximately 10 mg were pre-cleaned for elemental analysis following methods described in Matthews et al. (2006). In brief, samples were ultrasonicated in MilliQ water, oxidized in a solution of 50:50 0.2M NaOH and 30% H₂O₂, reduced using hydrazine buffered in 50:50 mixture of 30% NH₄OH and 0.25M (NH₄)2C₆H₆O₇, and leached in 0.001M HNO₃, with sub-boiling heat baths and ultrasonication, and multiple MilliQ water rinses between each step. Following cleaning, samples were dissolved in 6 mL of 2% v/v HNO₃. High-resolution 0.5 mg samples were prepared in the same method with the exception that they were dissolved into 2.5 mL of 2% v/v HNO₃.</p>
<p><strong>Elemental Analyses:</strong> Measurements were carried out on a Thermo Finnigan Element 2 ICP-SFMS using low (m/∆m = 300) resolution (with the exception of P at medium (m/∆m = 4000) resolution) and operated in E-scan mode with an uptake rate of 100 µL/min. Internal standards of Co (for Ca and P), Rh (for Ba), and Bi (for Pb) were used to correct for signal drift over the course of the run, blank corrected, and then concentrations calculated using the calibration curves from the gravimetric standards. For quantification, ⁴³Ca, ³¹P, ²⁰⁸Pb, and ¹³⁸Ba were used. At least one additional isotope of each element was monitored to check for interferences. P, Pb, and Ba concentrations were standardized to the measured Ca concentrations. The %RSD for P/Ca, Pb/Ca, and Ba/Ca are 3.7%, 7.2%, and 5.5% or better, respectively, based on repeated measurements of a check standard throughout the sequence of sample measurements.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0610487 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0610487
completed
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Location_country
Site_Name
Lat
Lon
Species
Specimen_ID
Sample_ID
Measurement_type
Chronology_date
P_Ca
Pb_Ca
Ba_Ca
Thermo Finnigan Element 2 ICP-SFMS
theme
None, User defined
site
latitude
longitude
species scientific name (binomial)
sample identification
sample type
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Inductively Coupled Plasma Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Stable Isotopes and Strontium in Western Pacific Sclerosponges: Calibration and Applications to Paleoceanography
https://osprey.bco-dmo.org/project/816475
Stable Isotopes and Strontium in Western Pacific Sclerosponges: Calibration and Applications to Paleoceanography
<p><em>NSF Award Abstract:</em><br />
Proxy records are extremely useful for reconstructing paleoceanographic/ paleoclimate conditions and hence understanding oceanographic and climate change, although these data must be well-calibrated. However, proxy tools to evaluate annual-to-centennial timescale variability of the stable isotopic composition of dissolved inorganic carbon and oxygen in seawater over the past several centuries in the Pacific or to evaluate these variables and seawater temperature across depth have not been fully developed. The western Pacific plays a large role in tropical and global climate and is therefore an ideal location to pursue this research.</p>
<p>A scientist at the University of Pennsylvania will quantitatively calibrate the sclerosponge, Acanthocheatetes wellsi, skeletal d13C, d18O, and strontium/calcium (Sr/Ca) to evaluate calibrated proxy records on annual-to-interdecadal timescales. The calibrations will then be applied to two high-resolution 50-year stable isotope and Sr/Ca records and the interannual-to-interdecadal timescale variability in the longer records will be evaluated by comparing them to available instrumental, satellite, and model output data.</p>
<p>The results will be a valuable tool for constraining existing carbon uptake models, initializing and testing ENSO models, and generally increasing our knowledge of the natural spatial and temporal variability in d13CDIC, d18Osw, and seawater temperature at the surface and across depth in the tropics.</p>
Sclerosponges
largerWorkCitation
project
eng; USA
oceans
134.517
145.1
7.267
15.033
2001-07-15
2003-07-15
0
BCO-DMO catalogue of parameters from Elemental ratios in Western Pacific sclerosponges
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/821992.rdf
Name: Location_country
Units: unitless
Description: <p>Location name</p>
http://lod.bco-dmo.org/id/dataset-parameter/821993.rdf
Name: Site_Name
Units: unitless
Description: <p>Site name</p>
http://lod.bco-dmo.org/id/dataset-parameter/821994.rdf
Name: Lat
Units: decimal degrees North
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/821995.rdf
Name: Lon
Units: decimal degrees East
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/821996.rdf
Name: Species
Units: unitless
Description: <p>Specie name</p>
http://lod.bco-dmo.org/id/dataset-parameter/821997.rdf
Name: Specimen_ID
Units: unitless
Description: <p>Specimen identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/821998.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/821999.rdf
Name: Measurement_type
Units: unitless
Description: <p>Type of measurement (bulk or high resolution)</p>
http://lod.bco-dmo.org/id/dataset-parameter/822000.rdf
Name: Chronology_date
Units: unitless
Description: <p>Chronology date; format: years and fraction of year (i.e., 30 January 2001 would be 2001.0822). Samples with dates of "nd" represent material that is integrated over the time period of 26 July 2001 - 15 July 2003 for samples from Palau and 15 July 2001-11 July 2003 for samples from Saipan.</p>
http://lod.bco-dmo.org/id/dataset-parameter/822001.rdf
Name: P_Ca
Units: micromoles per mole (umol/mol)
Description: <p>P/Ca</p>
http://lod.bco-dmo.org/id/dataset-parameter/822002.rdf
Name: Pb_Ca
Units: micromoles per mole (umol/mol)
Description: <p>Pb/Ca</p>
http://lod.bco-dmo.org/id/dataset-parameter/822003.rdf
Name: Ba_Ca
Units: micromoles per mole (umol/mol)
Description: <p>Ba/Ca</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7644
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/154990ae-7161-580f-95c6-e6b707c880b3/content
download
https://doi.org/10.26008/1912/bco-dmo.821982.1
download
onLine
dataset
<p>In the laboratory, each specimen was cut down its major growth axis, cleaned with deionized water, and dried at 60°C for 3 days. The sclerosponge&nbsp;skeletons were then sampled in two ways for elemental analysis. First, bulk measurements spanning the entire two-year common time period established by the stain lines were obtained from each specimen. Each bulk skeletal sample was milled from the growing edge to the stain line using a Dremmel tool fitted with a diamond-tipped dental drill bit. Second, high-resolution samples were milled at 0.5 mm increments using a Merchantek Micromill from two <em>A. wellsi</em> specimens from Palau, one <em>A. wellsi</em>specimens from Saipan, and one A. willeyana specimen from Saipan from the growing edge to the stain line.</p>
<p><strong>Sample Solutions and Standards:</strong> All solutions were made with MilliQ water (18 MΩ; Millipore, MA) and ultrapure reagents unless otherwise noted. All labware was pre-cleaned with 5% v/v HNO₃, 20% v/v HCl and MilliQ water for a minimum of 10 hours each prior to sample handling in a Laminar Flow Exhausting Hood. Gravimetric standards were used to make calibration curves for P, Pb, Ba, and Ca. Stock standard solutions (CPI International) were diluted with ultrapure 2% v/v HNO₃ to concentrations that matched expected range of sample concentrations.</p>
<p><strong>Sample Preparation:</strong> Bulk sclerosponge samples of approximately 10 mg were pre-cleaned for elemental analysis following methods described in Matthews et al. (2006). In brief, samples were ultrasonicated in MilliQ water, oxidized in a solution of 50:50 0.2M NaOH and 30% H₂O₂, reduced using hydrazine buffered in 50:50 mixture of 30% NH₄OH and 0.25M (NH₄)2C₆H₆O₇, and leached in 0.001M HNO₃, with sub-boiling heat baths and ultrasonication, and multiple MilliQ water rinses between each step. Following cleaning, samples were dissolved in 6 mL of 2% v/v HNO₃. High-resolution 0.5 mg samples were prepared in the same method with the exception that they were dissolved into 2.5 mL of 2% v/v HNO₃.</p>
<p><strong>Elemental Analyses:</strong> Measurements were carried out on a Thermo Finnigan Element 2 ICP-SFMS using low (m/∆m = 300) resolution (with the exception of P at medium (m/∆m = 4000) resolution) and operated in E-scan mode with an uptake rate of 100 µL/min. Internal standards of Co (for Ca and P), Rh (for Ba), and Bi (for Pb) were used to correct for signal drift over the course of the run, blank corrected, and then concentrations calculated using the calibration curves from the gravimetric standards. For quantification, ⁴³Ca, ³¹P, ²⁰⁸Pb, and ¹³⁸Ba were used. At least one additional isotope of each element was monitored to check for interferences. P, Pb, and Ba concentrations were standardized to the measured Ca concentrations. The %RSD for P/Ca, Pb/Ca, and Ba/Ca are 3.7%, 7.2%, and 5.5% or better, respectively, based on repeated measurements of a check standard throughout the sequence of sample measurements.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- renamed fields;<br />
- replaced "n/a" with "nd" as missing data value;<br />
- split latitude and longitude into separate columns;<br />
- converted latitude and longitude from degrees and minutes to decimal degrees (rounded to 3 decimal places);<br />
- replaced commas with semi-colons in&nbsp;Location_country column.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Thermo Finnigan Element 2 ICP-SFMS
Thermo Finnigan Element 2 ICP-SFMS
PI Supplied Instrument Name: Thermo Finnigan Element 2 ICP-SFMS PI Supplied Instrument Description:Thermo Finnigan Element 2 Inductively Coupled Plasma-Sector Field Mass Spectormeter Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/