http://lod.bco-dmo.org/id/dataset/822037
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Silicon Uptake Kinetics sampled aboard the R/V Pelican during during PE17-04 and PE17-20 along the Northern Gulf of Mexico, specifically the Louisiana Shelf region dominated by the discharge of the Mississippi River plume.
2020-08-31
publication
2020-08-31
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-09-03
publication
https://doi.org/10.26008/1912/bco-dmo.822037.1
Jeffrey W. Krause
Dauphin Island Sea Lab
principalInvestigator
Kanchan Maiti
Louisiana State University Dept. of Oceanography and Coastal Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Krause, J., Acton, S., Marquez Jr., I., Maiti, K. (2020) Silicon Uptake Kinetics sampled aboard the R/V Pelican during during PE17-04 and PE17-20 along the Northern Gulf of Mexico, specifically the Louisiana Shelf region dominated by the discharge of the Mississippi River plume. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-31 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.822037.1 [access date]
Methods and Sampling: <p>Hydrocasts were conducted at identified stations. A SeaBird CTD and rosette system, owned and maintained by Louisiana Universities Marine Consortium (LUMCON), operating institution for the R/V Pelican, was used for sampling. Calibration information can be found associated with the CTD data. Unless otherwise stated, samples used for rate measurements were collected based on the percent irradiance relative to that just below the surface.</p>
<p>Water was sampled from Niskin bottles and pooled into 10 L acid-cleaned carboys. For inorganic nutrients, water was filtered using 0.6 μm pore size polycarbonate membrane and immediately frozen until analysis. Filtered water was analyzed for dissolved silicic acid (Si(OH)4) using a manual colorimetric method (Krause et al. 2009). For biogenic silica analysis, seawater was filtered through a 1.2 μm-pore polycarbonate filter (47 mm diameter) and frozen immediately. On shore, filters were dried and analyzed using a sodium-carbonate time course digestion, to correct for lithogenic silica interference, in polymethylpentene tubes (Pickering et al. in review). In 2016, NaOH digestions were also done for biogenic silica, followed by an HF digestion to quantify lithogenic silica as in Krause et al. (2009).</p>
<p>Diatom rate processes were quantified using a radioisotope (32Si) and fluorescent dye (PDMPO) tracers. Samples bottles were incubated for &lt;12 hours in acrylic incubators cooled with continually flowing surface water under a series of neutral density screens to simulate light levels at the depth of collection (i.e. see above). Kinetic experiments were set up by filling eight 250-mL bottles and then adding increasing enrichments of Si(OH)4 (ambient to +20 μM). The rate of biogenic silica production was measured using the radioisotope tracer 32Si with high specific activity (&gt;40 kBq µg Si-1) as described in Krause et al. (2011). For PDMPO uptake, dye was added to samples at enriched in Si(OH)4 as described above, incubated in the same conditions as the 32Si samples, and processed as in McNair et al. (2015).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1558957 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1558957
completed
Jeffrey W. Krause
Dauphin Island Sea Lab
251-861-2141 x2289
101 Bienville Blvd
Dauphin Island
AL
36528
USA
jkrause@disl.edu
pointOfContact
Kanchan Maiti
Louisiana State University Dept. of Oceanography and Coastal Science
225-578-4531
1143 Energy Coast Env Bldg
Baton Rouge
LA
70803
USA
kmaiti@lsu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Cast_Number
Latitude
Longitude
Date_Local
Time_Local
ISO_DateTime_Local
Station_Number
Depth
bSi
Total_Silicate
Ave_Incubation_Time
Uptake_32Si_rho
Uptake_32Si_Vb
PDMPO_Blank
PDMPO_Uptake_rho
Trilogy fluorometer (Turner Designs)
GM 25 Multicounters (Risø National Laboratory, Technical University of Denmark)
theme
None, User defined
cruise name
cast
latitude
longitude
date_local
time_local
ISO_DateTime_Local
station
depth
biogenic silica concentration
Silicate (reactive silica)
incubation time or duration
rate of biogenic silica production
specific rate of biogenic silica production
instrument_blank
featureType
BCO-DMO Standard Parameters
Fluorometer
GM multicounter
instrument
BCO-DMO Standard Instruments
PE17-20
PE17-04
service
Deployment Activity
Northern Gulf of Mexico Shelf
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The biotic and abiotic controls on the Silicon cycle in the northern Gulf of Mexico
https://osprey.bco-dmo.org/project/712667
The biotic and abiotic controls on the Silicon cycle in the northern Gulf of Mexico
<p><em>NSF Award Abstract:</em><br />
The Louisiana Shelf system in the northern Gulf of Mexico is fed by the Mississippi River and its many tributaries which contribute large quantities of nutrients from agricultural fertilizer to the region. Input of these nutrients, especially nitrogen, has led to eutrophication. Eutrophication is the process wherein a body of water such as the Louisiana Shelf becomes enriched in dissolved nutrients that increase phytoplankton growth which eventually leads to decreased oxygen levels in bottom waters. This has certainly been observed in this area, and diatoms, a phytoplankton which represents the base of the food chain, have shown variable silicon/nitrogen (Si/N) ratios. Because diatoms create their shells from silicon, their growth is controlled not only by nitrogen inputs but the availability of silicon. Lower Si/N ratios are showing that silicon may be playing an increasingly important role in regulating diatom production in the system. For this reason, a scientist from the University of South Alabama will determine the biogeochemical processes controlling changes in Si/N ratios in the Louisiana Shelf system. One graduate student on their way to a doctorate degree and three undergraduate students will be supported and trained as part of this project. Also, four scholarships for low-income, high school students from Title 1 schools will get to participate in a month-long summer Marine Science course at the Dauphin Island Sea Laboratory and be included in the research project. The study has significant societal benefits given this is an area where $2.4 trillion gross domestic product revenue is tied up in coastal resources. Since diatoms are at the base of the food chain that is the biotic control on said coastal resources, the growth of diatoms in response to eutrophication is important to study.</p>
<p>Eutrophication of the Mississippi River and its tributaries has the potential to alter the biological landscape of the Louisiana Shelf system in the northern Gulf of Mexico by influencing the Si/N ratios below those that are optimal for diatom growth. A scientist from the University of South Alabama believes the observed changes in the Si/N ratio may indicate silicon now plays an important role in regulating diatom production in the system. As such, understanding the biotic and abiotic processes controlling the silicon cycle is crucial because diatoms dominate at the base of the food chain in this highly productive region. The study will focus on following issues: (1) the importance of recycled silicon sources on diatom production; (2) can heavily-silicified diatoms adapt to changing Si/N ratios more effectively than lightly-silicified diatoms; and (3) the role of reverse weathering in sequestering silicon thereby reducing diffusive pore-water transport. To attain these goals, a new analytical approach, the PDMPO method (compound 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylamino-carbamoyl)methoxy)phenyl)oxazole) that quantitatively measures taxa-specific silica production would be used.</p>
CLASiC
largerWorkCitation
project
eng; USA
oceans
Northern Gulf of Mexico Shelf
-91.6109
-89.74933
28.49727
29.06997
2016-08-29
2017-05-12
Northern Gulf of Mexico, specifically the Louisiana Shelf region dominated by the discharge of the Mississippi River on the western side of the delta
0
BCO-DMO catalogue of parameters from Silicon Uptake Kinetics sampled aboard the R/V Pelican during during PE17-04 and PE17-20 along the Northern Gulf of Mexico, specifically the Louisiana Shelf region dominated by the discharge of the Mississippi River plume.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/822771.rdf
Name: Cruise
Units: unitless
Description: <p>Name of specific cruise, no units</p>
http://lod.bco-dmo.org/id/dataset-parameter/822772.rdf
Name: Cast_Number
Units: unitless
Description: <p>CTD Number (chronological)</p>
http://lod.bco-dmo.org/id/dataset-parameter/822773.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of hydrocast</p>
http://lod.bco-dmo.org/id/dataset-parameter/822774.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of hydrocast</p>
http://lod.bco-dmo.org/id/dataset-parameter/822775.rdf
Name: Date_Local
Units: yyyy-mm-dd
Description: <p>Local date of hydrocast</p>
http://lod.bco-dmo.org/id/dataset-parameter/822776.rdf
Name: Time_Local
Units: hh:mm
Description: <p>Local time of hydrocast</p>
http://lod.bco-dmo.org/id/dataset-parameter/823081.rdf
Name: ISO_DateTime_Local
Units: YYYY-MM-DDTHH:MM
Description: <p>Date/Time (Local) ISO formatted</p>
http://lod.bco-dmo.org/id/dataset-parameter/823082.rdf
Name: Station_Number
Units: unitless
Description: <p>Specific to cruise</p>
http://lod.bco-dmo.org/id/dataset-parameter/823083.rdf
Name: Depth
Units: meter (m)
Description: <p>experiment sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/823084.rdf
Name: bSi
Units: umol Si/L
Description: <p>particulate biogenic silica</p>
http://lod.bco-dmo.org/id/dataset-parameter/823085.rdf
Name: Total_Silicate
Units: umol/L
Description: <p>dissolved silicate from ambient (lowest) to +20 uM</p>
http://lod.bco-dmo.org/id/dataset-parameter/823086.rdf
Name: Ave_Incubation_Time
Units: days
Description: <p>experimental incubation time, averaged among samples</p>
http://lod.bco-dmo.org/id/dataset-parameter/823087.rdf
Name: Uptake_32Si_rho
Units: umol Si/L/d
Description: <p>32Si-based Gross biogenic silica production</p>
http://lod.bco-dmo.org/id/dataset-parameter/823088.rdf
Name: Uptake_32Si_Vb
Units: d-1
Description: <p>32Si-based Biomass-normalized biogenic silica production</p>
http://lod.bco-dmo.org/id/dataset-parameter/823089.rdf
Name: PDMPO_Blank
Units: RFU
Description: <p>PDMPO-blank value at associated dissolved silicate</p>
http://lod.bco-dmo.org/id/dataset-parameter/823090.rdf
Name: PDMPO_Uptake_rho
Units: nmol PDMPO/L/d
Description: <p>PDMPO-based Gross biogenic silica production proxy</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5782
https://darchive.mblwhoilibrary.org/bitstream/1912/26160/1/dataset-822037_clasic-si-kinetics__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.822037.1
download
onLine
dataset
<p>Hydrocasts were conducted at identified stations. A SeaBird CTD and rosette system, owned and maintained by Louisiana Universities Marine Consortium (LUMCON), operating institution for the R/V Pelican, was used for sampling. Calibration information can be found associated with the CTD data. Unless otherwise stated, samples used for rate measurements were collected based on the percent irradiance relative to that just below the surface.</p>
<p>Water was sampled from Niskin bottles and pooled into 10 L acid-cleaned carboys. For inorganic nutrients, water was filtered using 0.6 μm pore size polycarbonate membrane and immediately frozen until analysis. Filtered water was analyzed for dissolved silicic acid (Si(OH)4) using a manual colorimetric method (Krause et al. 2009). For biogenic silica analysis, seawater was filtered through a 1.2 μm-pore polycarbonate filter (47 mm diameter) and frozen immediately. On shore, filters were dried and analyzed using a sodium-carbonate time course digestion, to correct for lithogenic silica interference, in polymethylpentene tubes (Pickering et al. in review). In 2016, NaOH digestions were also done for biogenic silica, followed by an HF digestion to quantify lithogenic silica as in Krause et al. (2009).</p>
<p>Diatom rate processes were quantified using a radioisotope (32Si) and fluorescent dye (PDMPO) tracers. Samples bottles were incubated for &lt;12 hours in acrylic incubators cooled with continually flowing surface water under a series of neutral density screens to simulate light levels at the depth of collection (i.e. see above). Kinetic experiments were set up by filling eight 250-mL bottles and then adding increasing enrichments of Si(OH)4 (ambient to +20 μM). The rate of biogenic silica production was measured using the radioisotope tracer 32Si with high specific activity (&gt;40 kBq µg Si-1) as described in Krause et al. (2011). For PDMPO uptake, dye was added to samples at enriched in Si(OH)4 as described above, incubated in the same conditions as the 32Si samples, and processed as in McNair et al. (2015).</p>
Specified by the Principal Investigator(s)
<p>Data were processed in Microsoft Excel.</p>
<p>BCO-DMO Data Manager Processing Notes:<br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* blank values in this dataset are displayed as "nd" for "no data."&nbsp; nd is the default missing data identifier in the BCO-DMO system.<br />
* removed all spaces in headers and replaced with underscores<br />
* removed all units from headers<br />
* converted dates to ISO Format yyyy-mm-dd<br />
* merged Date_Local and Time_Local to create ISO_DateTime_Local<br />
* set Types for each data column</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Trilogy fluorometer (Turner Designs)
Trilogy fluorometer (Turner Designs)
PI Supplied Instrument Name: Trilogy fluorometer (Turner Designs) PI Supplied Instrument Description:Quantification of bulk PDMPO was done using a Trilogy fluorometer (Turner Designs) using a crude-oil module. The sample matrix was hydrofluoric acid and boric acid. PDMPO was calibrated using a sequential addition of stock dye purchased from the vendor. Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
GM 25 Multicounters (Risø National Laboratory, Technical University of Denmark)
GM 25 Multicounters (Risø National Laboratory, Technical University of Denmark)
PI Supplied Instrument Name: GM 25 Multicounters (Risø National Laboratory, Technical University of Denmark) PI Supplied Instrument Description:Quantification of 32Si activity was done on a GM 25 Multicounters (Risø National Laboratory, Technical University of Denmark), each are configured to analyze five samples simultaneously. System setup includes an anti-coincidence module, which with considerable lead shielding reduces background activity to Instrument Name: GM multicounter Instrument Short Name: Instrument Description: A gas flow multicounter (GM multicounter) is used for counting low-level beta doses. GM multicounters can be used for gas proportional counting of 32Si to 32P. For more information about GM multicounter usage see Krause et. al. 2011.
Cruise: PE17-20
PE17-20
R/V Pelican
Community Standard Description
International Council for the Exploration of the Sea
R/V Pelican
vessel
PE17-20
Jeffrey W. Krause
Dauphin Island Sea Lab
Cruise: PE17-04
PE17-04
R/V Pelican
Community Standard Description
International Council for the Exploration of the Sea
R/V Pelican
vessel
PE17-04
Jeffrey W. Krause
Dauphin Island Sea Lab
R/V Pelican
Community Standard Description
International Council for the Exploration of the Sea
R/V Pelican
vessel