http://lod.bco-dmo.org/id/dataset/822239
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-08-27
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbon and Nitrogen isotopes in an experimental benthic chamber, Maunalua, O’ahu, Hawai’i, 2015-2016
2020-08-27
publication
2020-08-27
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-09-09
publication
https://doi.org/10.26008/1912/bco-dmo.822239.1
Craig E. Nelson
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Nelson, C. (2020) Carbon and Nitrogen isotopes in an experimental benthic chamber, Maunalua, O’ahu, Hawai’i, 2015-2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-08-27 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.822239.1 [access date]
CN isotopes Dataset Description: <p>This dataset carbon and nitrogen isotope ratios&nbsp;from experiments conducted at the University of Hawaii, Manoa in 2015.</p>
<p>Experiment CRANE (Coral Reef Acclimation to Nutrient Enrichment) identifies a month-long mesocosm incubation study designed to understand the response of the coral reef community to long-term nutrient exposure.</p> Methods and Sampling: <table border="0" cellpadding="0" cellspacing="0">
<tbody>
<tr>
<td><strong>Parameter</strong></td>
<td><strong>Method</strong></td>
</tr>
<tr>
<td>Final_mass_algae_g</td>
<td>All of the algal biomass was taken from the benthos covered by the benthic chamber at the end of the experiment. Algae was taken back to the lab, sorted by species, cleaned of sediment and epiphytes with DI water. Algae was dried at 60 degrees Celsius for 3 days and a final dry weight was taken by species.&nbsp;</td>
</tr>
<tr>
<td>L_filtered</td>
<td>0.5-1.14L of seawater was taken out of the benthic chamber and filtered through a 0.2 um previously combusted glass fiber filter.</td>
</tr>
<tr>
<td>Weight_mg</td>
<td>Sample is weighed during processing using a microbalance.</td>
</tr>
<tr>
<td>C, N</td>
<td>Algal and phytoplankton samples were dried for 3 days at 60 degrees Celsius.&nbsp; Analysis of samples for carbon [ug C] and nitrogen [ug N], and isotopic composition [d13C (permil vs. PDB) and d15N (permil vs. AIR) ] was done using the following instrumentation: Costech ECS 4010 Elemental Combustion System/Zero Blank Autosampler /ThermoFinnigan MAT Conflo IV/ThermoFinnigan&nbsp; DeltaXP.</td>
</tr>
</tbody>
</table>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1538393 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1538393
completed
Craig E. Nelson
University of Hawaiʻi at Mānoa
805-705-3497
Center for Microbial Oceanography: Research & Education Dept of Oceanography & UH Sea Grant
Honolulu
HI
96822
USA
cen@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Exp_ID
sample_id
species
Ambient_or_Enriched
Final_mass_algae_g
L_filtered
Weight_mg
ug_N
d15N_permil_vs_AIR
ug_C
d13C_permil_vs_PDB
Costech ECS 4010 Elemental Combustion System/Zero Blank Autosampler /ThermoFinnigan MAT Conflo IV/ThermoFinnigan DeltaXP
theme
None, User defined
experiment id
sample identification
taxon
sample description
mass
volume of water filtered
weight
Nitrogen
d15N
Elemental carbon (C)
d13C
featureType
BCO-DMO Standard Parameters
Laboratory Autosampler
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Dissolved organic matter feedbacks in coral reef resilience: The genomic & geochemical basis for microbial modulation of algal phase shifts
https://osprey.bco-dmo.org/project/675025
Collaborative Research: Dissolved organic matter feedbacks in coral reef resilience: The genomic & geochemical basis for microbial modulation of algal phase shifts
<p><em>NSF award abstract:</em><br />
Coral reef degradation, whether driven by overfishing, nutrient pollution, declining water quality, or other anthropogenic factors, is associated with a phase shift towards a reefs dominated by fleshy algae. In many cases managing and ameliorating these stressors does not lead to a return to coral dominance, and reefs languish in an algal-dominated state for years. Nearly a decade of research has demonstrated that trajectories toward increasing algal dominance are restructuring microbial community composition and metabolism; the investigators hypothesize that microbial processes facilitate the maintenance of algal dominance by metabolizing organic compounds released by algae thereby stressing corals through hypoxia and disease. The resilience of reefs to these phase shifts is a critical question in coral reef ecology, and managing reefs undergoing these community shifts requires developing an understanding of the role of microbial interactions in facilitating algal overgrowth and altering reef ecosystem function. The research proposed here will investigate the organics produced by algae, the microbes that metabolize the organics, and the impacts of these processes on coral health and growth. This research has implications for managing reef resilience to algal phase shifts by testing the differential resistance of coral-associated microbial communities to algae and defining thresholds of algal species cover which alter ecosystem biogeochemistry. This project provides mentoring across multiple career levels, linking underrepresented undergraduates, two graduate students, a postdoctoral researcher, and a beginning and established investigators.</p>
<p>This project will integrate dissolved organic matter (DOM) geochemistry, microbial genomics and ecosystem process measurements at ecologically-relevant spatial and temporal scales to test hypothetical mechanisms by which microbially-mediated feedbacks may facilitate the spread of fleshy algae on Pacific reef ecosystems. A key product of this research will be understanding how the composition of corals and algae on reefs interact synergistically with complex microbial communities to influence reef ecosystem resilience to algal phase shifts. Emerging molecular and biogeochemical methods will be use to investigate mechanisms of microbial-DOM interactions at multiple spatial and temporal scales. This project will leverage the background environmental data, laboratory facilities and field logistical resources of the Mo'orea Coral Reef Long Term Ecological Research Project in French Polynesia and contribute to the mission of that program of investigating coral reef resilience in the face of global change. The investigators will quantify bulk diel patterns of DOM production and characterize the composition of chromophoric components and both free and acid-hydrolyzable neutral monosaccharides and amino acids from varying benthic algae sources. The team will also characterize planktonic and coral-associated microbial community changes in taxonomic composition and gene expression caused by algal DOM amendments in on-site controlled environmental chambers using phylogenetics and metatranscriptomics, including tracking algal exudate utilization by specific microbial lineages. Field-deployed 100 liter tent mesocosms will be used to examine in situ diel patterns of coupled DOM production and consumption, microbial community genomics and ecosystem metabolism over representative benthic communities comprising combinations of algal and coral species. Together these experimental results will guide interpretation of field surveys of centimeter-scale spatial dynamics of planktonic and coral-associated microbial genomics and metabolism at zones of coral-algal interaction, including boundary layer dynamics of oxygen, bacteria and DOM using planar optodes, high-throughput flow cytometry and fluorescence spectroscopy.</p>
Coral DOM2
largerWorkCitation
project
eng; USA
oceans
-157.76335
-157.760472
21.27273
21.275824
2015-10-09
2016-11-03
Pacific Coral Reefs
0
BCO-DMO catalogue of parameters from Carbon and Nitrogen isotopes in an experimental benthic chamber, Maunalua, O’ahu, Hawai’i, 2015-2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/823064.rdf
Name: Exp_ID
Units: NA
Description: <p>Identification for experiment; corresponds to Exp ID in La Valle_Wailupe productivity macroalgae and phytoplankton_2015-2016.xlsb</p>
http://lod.bco-dmo.org/id/dataset-parameter/823065.rdf
Name: sample_id
Units: NA
Description: <p>Identification for isotope sample (algal tissue or phytoplankton on a filer).</p>
http://lod.bco-dmo.org/id/dataset-parameter/823066.rdf
Name: species
Units: NA
Description: <p>Macroalgal species or phytoplankton designation</p>
http://lod.bco-dmo.org/id/dataset-parameter/823067.rdf
Name: Ambient_or_Enriched
Units: NA
Description: <p>Ambient refers to a sample taken before the enrichment at the beginning of the experiment. Enriched refers to a sample taken at the end of the experiment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/823068.rdf
Name: Final_mass_algae_g
Units: gram (g)
Description: <p>Final dry weight of algal species in the benthic chamber. No value for phytoplankton.</p>
http://lod.bco-dmo.org/id/dataset-parameter/823069.rdf
Name: L_filtered
Units: Liter (L)
Description: <p>Sea water volume filtered.</p>
http://lod.bco-dmo.org/id/dataset-parameter/823070.rdf
Name: Weight_mg
Units: milligram (mg)
Description: <p>weight of sample given by lab</p>
http://lod.bco-dmo.org/id/dataset-parameter/823071.rdf
Name: ug_N
Units: micrograms of Nitrogen (ug N)
Description: <p>concentration of nitrogen sample given by lab</p>
http://lod.bco-dmo.org/id/dataset-parameter/823072.rdf
Name: d15N_permil_vs_AIR
Units: unitless
Description: <p>delta 15N of sample given by lab; permil vs. air</p>
http://lod.bco-dmo.org/id/dataset-parameter/823073.rdf
Name: ug_C
Units: micrograms of Carbon (ug C)
Description: <p>concentration of carbon sample given by lab</p>
http://lod.bco-dmo.org/id/dataset-parameter/823074.rdf
Name: d13C_permil_vs_PDB
Units: unitless
Description: <p>delta 13C of sample given by lab; permil vs. PDB</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11479
https://darchive.mblwhoilibrary.org/bitstream/1912/26169/1/dataset-822239_c-and-n-isotopes__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.822239.1
download
onLine
dataset
<table border="0" cellpadding="0" cellspacing="0">
<tbody>
<tr>
<td><strong>Parameter</strong></td>
<td><strong>Method</strong></td>
</tr>
<tr>
<td>Final_mass_algae_g</td>
<td>All of the algal biomass was taken from the benthos covered by the benthic chamber at the end of the experiment. Algae was taken back to the lab, sorted by species, cleaned of sediment and epiphytes with DI water. Algae was dried at 60 degrees Celsius for 3 days and a final dry weight was taken by species.&nbsp;</td>
</tr>
<tr>
<td>L_filtered</td>
<td>0.5-1.14L of seawater was taken out of the benthic chamber and filtered through a 0.2 um previously combusted glass fiber filter.</td>
</tr>
<tr>
<td>Weight_mg</td>
<td>Sample is weighed during processing using a microbalance.</td>
</tr>
<tr>
<td>C, N</td>
<td>Algal and phytoplankton samples were dried for 3 days at 60 degrees Celsius.&nbsp; Analysis of samples for carbon [ug C] and nitrogen [ug N], and isotopic composition [d13C (permil vs. PDB) and d15N (permil vs. AIR) ] was done using the following instrumentation: Costech ECS 4010 Elemental Combustion System/Zero Blank Autosampler /ThermoFinnigan MAT Conflo IV/ThermoFinnigan&nbsp; DeltaXP.</td>
</tr>
</tbody>
</table>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech ECS 4010 Elemental Combustion System/Zero Blank Autosampler /ThermoFinnigan MAT Conflo IV/ThermoFinnigan DeltaXP
Costech ECS 4010 Elemental Combustion System/Zero Blank Autosampler /ThermoFinnigan MAT Conflo IV/ThermoFinnigan DeltaXP
PI Supplied Instrument Name: Costech ECS 4010 Elemental Combustion System/Zero Blank Autosampler /ThermoFinnigan MAT Conflo IV/ThermoFinnigan DeltaXP PI Supplied Instrument Description:For analysis of samples for carbon [_g C] and nitrogen [_g N], and isotopic composition [d13C (â vs. PDB) and d15N (â vs. AIR) ]. Instrument Name: Laboratory Autosampler Instrument Short Name: Instrument Description: Laboratory apparatus that automatically introduces one or more samples with a predetermined volume or mass into an analytical instrument.