http://lod.bco-dmo.org/id/dataset/824623
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-09-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Bacterial cell counts and Dissolved Organic Carbon (DOC) measurements from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018
2020-09-16
publication
2020-09-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-09-22
publication
https://doi.org/10.26008/1912/bco-dmo.824623.1
Craig A. Carlson
University of California-Santa Barbara
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Carlson, C. (2020) Bacterial cell counts and Dissolved Organic Carbon (DOC) measurements from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-09-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.824623.1 [access date]
bacterial abundance and DOC Dataset Description: <p>This dataset includes analyses from Niskin bottle samples collected on R/V Atlantis cruises AT32, AT34, AT38 and AT39-6 as part of the NASA NAAMES campaign (2015-2018). Reported are bacterial abundances and Dissolved Organic Carbon (DOC) measurements pre- and post-experiment. Remineralization experiments were used to examine the ability of natural assemblages of bacteria to utilize in situ DOM as well as different algal-derived substrates.</p> Methods and Sampling: <p>Bioassays were conducted at each station using water collected within 10 m depth horizon. Water was gently gravity-filtered serially through 142 mm PC filtration cartridges loaded with either a 1.2- or a 0.2-µm mixed cellulose ester membrane filter. The 1.2 µm filtrate was retained as a bacterioplankton inoculum with diluted large phytoplankton and nanoflagellate grazers while the 0.2 µm fraction was retained as particle-free DOM substrate. When possible, the filtration rig was directly attached to Niskin bottles with platinum-cured silicon tubing and the filtrate was collected into PC carboys. Otherwise, unfiltered water from the Niskin was first drawn into a PC carboy and then filtered into another PC carboy. Each bioassay was initiated by combining the 1.2 µm filtrate with the 0.2 µm filtrate in a 30:70 ratio. A pair of incubation bottles were then rinsed with this water and subsequently filled. Ammendments were added if applicable. 8 pre-combusted (4 hours at 450°C) 40 mL EPA borosilicate glass incubation vials were also rinsed and filled with initial incubation water. Bioassays were maintained in Fisherbrand Isotemp BOD refrigerated incubators. They were kept in darkness for up to 81 days at temperatures as near in situ as logistically possible (±1.5°C). Incubation bottles were sampled over the duration of each campaign, while vials were sacrificially sampled for organic carbon during and beyond the duration of each campaign. Bacterioplankton carbon (BC, µmol C / L) refers to the carbon content of a population at any given time. BC at the initial and stationary growth conditions of each bioassay were estimated by applying empirical carbon conversion factors (CCFs, fg C / cell) to POC estimates. The dissolved organic carbon (DOC) fraction in a bioassay, was estimated by subtracting bacterioplankton cell carbon (µmol C / L) from TOC measurements.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1537943 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1537943
completed
Craig A. Carlson
University of California-Santa Barbara
(805) 893-2541
University of California Department of Ecology, Evolution and Marine Biology
Santa Barbara
CA
93106-6150
USA
craig_carlson@ucsb.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Station
Depth
Treatment
Bottle
Hours
Stationary_Sampling
Cells
Cells_sd
DOC
DOC_sd
Initial_BC
Stationary_BC
Olympus BX51 Epiflourescence Microscope
Fisherbrand Isotemp BOD refrigerated incubators
theme
None, User defined
Cruise identfier
station
depth
treatment
bottle
time_elapsed
time of day
bacterial abundance
dissolved organic Carbon
Elemental carbon (C)
featureType
BCO-DMO Standard Parameters
Fluorescence Microscope
Niskin bottle
Shimadzu TOC-V Analyzer
Shipboard Incubator
instrument
BCO-DMO Standard Instruments
AT34
AT38
AT39-06
service
Deployment Activity
N. Atlantic
North Atlantic Ocean (42°N to 54°N; 39-45°W)
NW Atlantic
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
North Atlantic Aerosols and Marine Ecosystems Study
http://naames.larc.nasa.gov/
North Atlantic Aerosols and Marine Ecosystems Study
The North Atlantic Aerosols and Marine Ecosystems Study (NAAMES) is an interdisciplinary investigation resolving key processes controlling marine ecosystems and aerosols that are essential to our understanding of Earth system function and future change. NAAMES is funded by the NASA Earth Venture Suborbital Program and is the first EV-S mission focused on studying the coupled ocean ecosystem and atmosphere.
Plankton ecosystems of the global ocean profoundly affect climate and life on Earth. NASA's ocean color satellite record tells us that these invaluable ecosystems are highly responsive to climate variability, with changes in ocean production impacting food production, uptake of atmospheric carbon dioxide, and emission of climate-regulating aerosols. Intergovernmental Panel on Climate Change (IPCC) simulations suggest that surface ocean temperatures will warm by +1.3 to +2.8 degrees C globally over the 21st century, with major consequences on physical properties of the surface ocean where plankton populations thrive. The pressing question is, how will these changes alter plankton production, species composition, and aerosol emissions? Today, even the sign of these potential changes remains unresolved. Our ability to predict Earth System consequences of a warming ocean and develop realistic mitigation and adaption strategies depends on resolving conflicting hypotheses regarding the factors controlling plankton ecosystems and biogenic aerosol emissions.
NAAMES consists of four, combined ship and aircraft field campaigns that are each aligned to a specific event in the annual plankton lifecycle. Ship-based measurements provide detailed characterization of plankton stocks, rate processes, and community composition. Ship measurements also characterize sea water volatile organic compounds, their processing by ocean ecosystems, and the concentrations and properties of gases and particles in the overlying atmosphere. These diverse data are extended over broader spatial scales through parallel airborne remote sensing measurements and in situ aerosol sampling that target ocean properties as well as the aerosols and clouds above. The airborne data crucially link local-scale processes and properties to the much larger scale continuous satellite record. Integrating the NAAMES observations with state-of-the-art climate and ecosystems models enables the creation of a process-based foundation for resolving plankton dynamics in other ocean regions, accurately interpreting historical satellite records, and improving predictions of future change and their societal impacts.
NAAMES
largerWorkCitation
program
Tracking the temporal and spatial variability of dissolved organic matter, its diagenetic state and bioavailability during various bloom states in the North Atlantic
https://osprey.bco-dmo.org/project/657725
Tracking the temporal and spatial variability of dissolved organic matter, its diagenetic state and bioavailability during various bloom states in the North Atlantic
<p>Tracking the temporal and spatial variability of dissolved organic matter, its diagenetic state and bioavailability during various bloom states in the North Atlantic<br />
Craig Carlson<br />
ID: 1537943</p>
<p>The North Atlantic phytoplankton bloom is among the most conspicuous biological events annually recorded. This bloom represents a hot spot of biological activity during which a significant amount of dissolved organic matter is produced through bloom-associated food web processes. While recent work has shed some light on the spatial distribution of dissolved organic matter during the North Atlantic bloom, temporal resolution of dissolved organic matter variability in the context of the North Atlantic bloom is lacking. This project aims to understand the temporal and spatial dynamics of dissolved organic matter, its compositional variability, as well as the mechanisms that control its accumulation, persistence and export in the North Atlantic. This project will leverage a large, recently funded, NASA field-program called the North Atlantic Aerosols and Marine Ecosystem Study (NAAMES) designed to evaluate the fundamental controls of the north Atlantic phytoplankton bloom initiation, its magnitude and interannual variability. Results from this research will provide a mechanistic understanding of carbon cycling in the context of the North Atlantic phytoplankton bloom. The research will be carried out at the University of California ? Santa Barbara, a Hispanic-serving institution, and will involve educational opportunities for students from elementary through graduate school.</p>
<p>Recent work examining the spatial distribution of dissolved organic matter in the North Atlantic coupled to measurements of water mass ventilation rates has estimated that a significant amount of carbon is vertically exported out of the surface ocean to deep waters as dissolved organic matter. However, an overarching gap in dissolved organic matter knowledge is the lack of valuable temporal resolution necessary to investigate the mechanisms that control dissolved organic matter production, accumulation, or its change in quality and bioavailability as a result of changing bloom phases and phytoplankton cycles. This research will examine the temporal and spatial variability of dissolved organic matter dynamics along a repeated meridional transect during four distinct phases associated with the North Atlantic spring phytoplankton bloom including 1) pre-bloom, mixing phase, 2) nutrient-replete, increasing biomass phase, 3) nutrient-stressed decreasing biomass phase, and 4) post bloom stratified phase. This will be accomplished by coupling continuous water column and surface layer ecosystem properties from autonomous in situ sensors, and satellite observations with four 26-day coordinated ship and airborne field campaigns.</p>
DOM_SeasonalDynamics
largerWorkCitation
project
eng; USA
biota
oceans
N. Atlantic; North Atlantic Ocean (42°N to 54°N; 39-45°W); NW Atlantic
-46.148
-37.514
39.187
56.341
2016-05-11
2018-03-20
North Atlantic Ocean (35°N to 57°N; 45°W)
0
BCO-DMO catalogue of parameters from Bacterial cell counts and Dissolved Organic Carbon (DOC) measurements from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/824896.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/824897.rdf
Name: Station
Units: unitless
Description: <p>Station identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/824898.rdf
Name: Depth
Units: meters
Description: <p>Depth from which incubation water was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/824899.rdf
Name: Treatment
Units: unitless
Description: <p>Indicates the type of amendment or mixture used (when applicable)</p>
http://lod.bco-dmo.org/id/dataset-parameter/824900.rdf
Name: Bottle
Units: unitless
Description: <p>ID for incubation bottle</p>
http://lod.bco-dmo.org/id/dataset-parameter/824901.rdf
Name: Hours
Units: hours
Description: <p>Time since experiment initiation</p>
http://lod.bco-dmo.org/id/dataset-parameter/824902.rdf
Name: Stationary_Sampling
Units: hours
Description: <p>Time at which sampling for POC took place</p>
http://lod.bco-dmo.org/id/dataset-parameter/824903.rdf
Name: Cells
Units: cells /Liter
Description: <p>Bacterial abundance measure by epifluorescent microscopy (Porter & Feig; 1980)</p>
http://lod.bco-dmo.org/id/dataset-parameter/824904.rdf
Name: Cells_sd
Units: cells /Liter
Description: <p>Bacterial abundance standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/824905.rdf
Name: DOC
Units: micromol carbon/liter (umol C / L)
Description: <p>Dissolved organic carbon concentration measured by high temperature combustion/oxidation (HTCO) (Carlson et al; 2010)</p>
http://lod.bco-dmo.org/id/dataset-parameter/824906.rdf
Name: DOC_sd
Units: micromol carbon/liter (umol C / L)
Description: <p>Dissolved organic carbon concentration standard deviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/824907.rdf
Name: Initial_BC
Units: micromol carbon/liter (umol C / L)
Description: <p>Bacterial carbon concentration at the start of the experiment measured by high temperature combustion/oxidation (HTCO ) with glass fiber filtrate type GF/75 (Advantec) ( ?James et al; 2017)</p>
http://lod.bco-dmo.org/id/dataset-parameter/824908.rdf
Name: Stationary_BC
Units: micromol carbon/liter (umol C / L)
Description: <p>Bacterial carbon concentration at the stationary sampling time measured by high temperature combustion/oxidation (HTCO ) with glass fiber filtrate type GF/75 (Advantec) ( ?James et al; 2017)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
56566
https://darchive.mblwhoilibrary.org/bitstream/1912/26208/1/dataset-824623_bacterial-cell-counts-and-doc__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.824623.1
download
onLine
dataset
<p>Bioassays were conducted at each station using water collected within 10 m depth horizon. Water was gently gravity-filtered serially through 142 mm PC filtration cartridges loaded with either a 1.2- or a 0.2-µm mixed cellulose ester membrane filter. The 1.2 µm filtrate was retained as a bacterioplankton inoculum with diluted large phytoplankton and nanoflagellate grazers while the 0.2 µm fraction was retained as particle-free DOM substrate. When possible, the filtration rig was directly attached to Niskin bottles with platinum-cured silicon tubing and the filtrate was collected into PC carboys. Otherwise, unfiltered water from the Niskin was first drawn into a PC carboy and then filtered into another PC carboy. Each bioassay was initiated by combining the 1.2 µm filtrate with the 0.2 µm filtrate in a 30:70 ratio. A pair of incubation bottles were then rinsed with this water and subsequently filled. Ammendments were added if applicable. 8 pre-combusted (4 hours at 450°C) 40 mL EPA borosilicate glass incubation vials were also rinsed and filled with initial incubation water. Bioassays were maintained in Fisherbrand Isotemp BOD refrigerated incubators. They were kept in darkness for up to 81 days at temperatures as near in situ as logistically possible (±1.5°C). Incubation bottles were sampled over the duration of each campaign, while vials were sacrificially sampled for organic carbon during and beyond the duration of each campaign. Bacterioplankton carbon (BC, µmol C / L) refers to the carbon content of a population at any given time. BC at the initial and stationary growth conditions of each bioassay were estimated by applying empirical carbon conversion factors (CCFs, fg C / cell) to POC estimates. The dissolved organic carbon (DOC) fraction in a bioassay, was estimated by subtracting bacterioplankton cell carbon (µmol C / L) from TOC measurements.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- replaced blank cells and -999 with 'nd' (no data)<br />
- concatenated tables from 3 cruises (AT34, AT38, AT39-05)</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Olympus BX51 Epiflourescence Microscope
Olympus BX51 Epiflourescence Microscope
PI Supplied Instrument Name: Olympus BX51 Epiflourescence Microscope PI Supplied Instrument Description:For bacterial abundance estimates Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
PI Supplied Instrument Name: PI Supplied Instrument Description:For water sample collection Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
PI Supplied Instrument Name: PI Supplied Instrument Description:Used to measure dissolved organic carbon (DOC) and total dissolved nitrogen (TDN).
Shimadzu TOC-V analyzers (Shimadzu Scientific Instruments, Columbia, MD, USA) were slightly modified from the manufacturer’s model system. The condensation coil was removed and the head space of an internal water trap was reduced to minimize system dead space. The combustion tube contained 0.5 cm Pt pillows placed on top of Pt alumina beads to improve peak shape and to reduce alteration of the combustion matrix throughout the analytical run. CO2-free carrier gas was delivered to the TOC-V systems via commercial ultra high purity gas cylinders or a Whatmans gas generator. A magnesium perchlorate trap was added to the existing water and halide traps to ensure removal of water vapor from the gas line prior to entering a nondispersive infrared detector. The resulting peak area was integrated with Shimadzu chromatographic software. Instrument Name: Shimadzu TOC-V Analyzer Instrument Short Name:Shimadzu TOC-V Instrument Description: A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method. Community Standard Description: http://onto.nerc.ac.uk/CAST/124
Fisherbrand Isotemp BOD refrigerated incubators
Fisherbrand Isotemp BOD refrigerated incubators
PI Supplied Instrument Name: Fisherbrand Isotemp BOD refrigerated incubators PI Supplied Instrument Description:Used for bioassays. Instrument Name: Shipboard Incubator Instrument Short Name: Instrument Description: A device mounted on a ship that holds water samples under conditions of controlled temperature or controlled temperature and illumination.
Cruise: AT34
AT34
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT34
Dr Michael J. Behrenfeld
Oregon State University
Cruise: AT38
AT38
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT38
Dr Michael J. Behrenfeld
Oregon State University
Cruise: AT39-06
AT39-06
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT39-06
Dr Michael J. Behrenfeld
Oregon State University
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel