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Low oxygen (hypoxia) and low pH are known to have profound physiological effects on zooplankton, the microscopic animals of the sea. It is likely that many individual zooplankton change vertical mirgration behaviors to reduce or avoid these stresses. However, avoidance responses and their consequences for zooplankton distributions, and for interactions of zooplankton with their predators and prey, are poorly understood. This study will provide information on small-scale behavioral responses of zooplankton to oxygen and pH using video systems deployed in the field in a seasonally hypoxic estuary. The results will deepen our understanding of how zooplankton respond to low oxygen and pH conditions in ways that could profoundly affect marine ecosystems and fisheries through changes in their populations and distributions. This project will train graduate students and will engage K-12 students and teachers in under-served coastal communities by developing ocean technology-based citizen-scientist activities and curricular materials in plankton ecology, ocean change, construction and use of biological sensors, and quantitative analysis of environmental data.&lt;/p&gt;
&lt;p&gt;Individual directional motility is a primary mechanism underlying spatio-temporal patterns in zooplankton population distributions. Motility is used by most zooplankton species to select among water column positions that differ in biotic and abiotic variables such as prey, predators, light, oxygen concentration, and pH. Species-specific movement responses to de-oxygenation and acidification are likely mechanisms through which short-term, localized impacts of these stressful conditions on individual zooplankton will be magnified or suppressed as they propagate up to population, community, and ecosystem-level dynamics. This study will quantify responses by key zooplankton species to oxygen and pH using in situ video systems to measure changes in individual behavior in hypoxic, low- pH versus well-oxygenated, high-pH regions of a seasonally hypoxic estuary. Distributions and movements of zooplankton will be quantified using three approaches: 1) an imaging system deployed in situ on a profiling mooring over two summers in a hypoxic region, 2) imagers deployed on Lagrangian drifters to sample simultaneously throughout the water column, and 3) vertically-stratified pumps and net tows to verify species identification and video-based abundance estimates. These field observations will be combined with laboratory analysis of zooplankton movements in oxygen and pH gradients, and with spatially-explicit models to predict how behavioral mechanisms lead to large-scale impacts of environmental stresses.&lt;/p&gt;
&lt;p&gt;The following deployments were conducted in 2017 and 2018:&lt;br /&gt;
CB1077: &lt;a href=&quot;https://www.bco-dmo.org/deployment/735746&quot; target=&quot;_blank&quot;&gt;https://www.bco-dmo.org/deployment/735746&lt;/a&gt;&lt;br /&gt;
CB1072: &lt;a href=&quot;https://www.bco-dmo.org/deployment/735748&quot; target=&quot;_blank&quot;&gt;https://www.bco-dmo.org/deployment/735748&lt;/a&gt;&lt;br /&gt;
Zoocam_ORCA_Twanoh_2017: &lt;a href=&quot;https://www.bco-dmo.org/deployment/735762&quot; target=&quot;_blank&quot;&gt;https://www.bco-dmo.org/deployment/735762&lt;/a&gt;&lt;br /&gt;
RC0008: &lt;a href=&quot;https://www.bco-dmo.org/deployment/775288&quot; target=&quot;_blank&quot;&gt;https://www.bco-dmo.org/deployment/775288&lt;/a&gt;&lt;br /&gt;
Mooring ORCA_Hoodsport; NANOOS-APL4: &lt;a href=&quot;https://www.bco-dmo.org/deployment/775291&quot; target=&quot;_blank&quot;&gt;https://www.bco-dmo.org/deployment/775291&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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	Description: &lt;p&gt;Year and month (UTC) of cruise&lt;/p&gt; 
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http://lod.bco-dmo.org/id/dataset-parameter/828442.rdf
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	Description: &lt;p&gt;Original file name&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828443.rdf
	Name: Density
	Units: kilograms per cubic meter (Kg/m^3)
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http://lod.bco-dmo.org/id/dataset-parameter/828444.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/828445.rdf
	Name: Fluorescence
	Units: milligrams per cubic meter (mg/m^3)
	Description: &lt;p&gt;Fluorescence, WET Labs ECO-AFL/FL&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828446.rdf
	Name: Latitude
	Units: degrees North
	Description: &lt;p&gt;Latitude&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828447.rdf
	Name: Longitude
	Units: degrees East
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http://lod.bco-dmo.org/id/dataset-parameter/828448.rdf
	Name: Oxygen_raw
	Units: volts (V)
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http://lod.bco-dmo.org/id/dataset-parameter/828449.rdf
	Name: Oxygen_SBE_43
	Units: milligrams per liter (mg/l)
	Description: &lt;p&gt;Oxygen, SBE 43&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828450.rdf
	Name: Oxygen_saturation_Garcia_Gordon
	Units: milligrams per liter (mg/l)
	Description: &lt;p&gt;Oxygen saturation, Garcia &amp;amp; Gordon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828451.rdf
	Name: Oxygen_saturation_Weiss
	Units: milligrams per liter (mg/l)
	Description: &lt;p&gt;Oxygen saturation, Weiss&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828452.rdf
	Name: PAR_Irradiance
	Units: micromoles photons per square meter per second (umol photons/m^2/s^1)
	Description: &lt;p&gt;PAR/ Irradiance, Biospherical/Licor&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828453.rdf
	Name: pH
	Units: unitless
	Description: &lt;p&gt;pH&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828454.rdf
	Name: Salinity_Practical
	Units: PSU
	Description: &lt;p&gt;Salinity, Practical&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828455.rdf
	Name: Sound_Velocity_Delgrosso
	Units: meters per second (m/s)
	Description: &lt;p&gt;Sound Velocity (Delgrosso)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828456.rdf
	Name: Temperature
	Units: degrees Celsius
	Description: &lt;p&gt;Temperature (ITS-9)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828457.rdf
	Name: Voltage_0
	Units: volts (V)
	Description: &lt;p&gt;Voltage 0&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828458.rdf
	Name: Voltage_1
	Units: volts (V)
	Description: &lt;p&gt;Voltage 1&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828459.rdf
	Name: Voltage_2
	Units: volts (V)
	Description: &lt;p&gt;Voltage 2&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828460.rdf
	Name: Voltage_3
	Units: volts (V)
	Description: &lt;p&gt;Voltage 3&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828461.rdf
	Name: Voltage_4
	Units: volts (V)
	Description: &lt;p&gt;Voltage 4&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828462.rdf
	Name: Voltage_5
	Units: volts (V)
	Description: &lt;p&gt;Voltage 5&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828463.rdf
	Name: Voltage_6
	Units: volts (V)
	Description: &lt;p&gt;Voltage 6&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828464.rdf
	Name: Voltage_7
	Units: volts (V)
	Description: &lt;p&gt;Voltage 7&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828465.rdf
	Name: Descent_Rate
	Units: meters per second (m/s)
	Description: &lt;p&gt;Descent Rate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828466.rdf
	Name: Pressure_Temperature
	Units: degrees Celsius
	Description: &lt;p&gt;Pressure Temperature&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828467.rdf
	Name: Pressure_db
	Units: decibars (db)
	Description: &lt;p&gt;Pressure, Digiquartz&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828468.rdf
	Name: Pressure_psi
	Units: psi
	Description: &lt;p&gt;Pressure, Digiquartz&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828469.rdf
	Name: Sound_Velocity_Chen_Millero
	Units: meters per second (m/s)
	Description: &lt;p&gt;Sound Velocity (Chen-Millero)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828470.rdf
	Name: Sound_Velocity_Wilson
	Units: meters per second (m/s)
	Description: &lt;p&gt;Sound Velocity (Wilson)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828471.rdf
	Name: Average_Sound_Velocity_Delgrosso
	Units: meters per second (m/s)
	Description: &lt;p&gt;Average Sound Velocity (Delgrosso)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828472.rdf
	Name: Oxygen_SBE_43_Aligned
	Units: milligrams per liter (mg/l)
	Description: &lt;p&gt;Oxygen, SBE 43, offset corrected&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/828473.rdf
	Name: Flag
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	Description: &lt;p&gt;flag&lt;/p&gt; 
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&amp;lt;p&amp;gt;Based on modified Winkler titrations, the formula y = 0.9144x + 0.1975 can be applied to the CTD data to calibrate the &amp;quot;Oxygen, SBE 43 (mg/l)&amp;quot; for the 2017 cruises. The formula y = 0.9698x + 0.0691 can be applied for the 2018 cruises.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BCO-DMO Processing:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
- concatenated data from separate cruises into one dataset;&amp;lt;br /&amp;gt;
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