Bulk soil and elemental properties of marsh and infilled pond soils collected in 2014-2015 within Plum Island Ecosystems LTER

Website: https://www.bco-dmo.org/dataset/827298
Data Type: Other Field Results
Version: 1
Version Date: 2020-11-13

» Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary (Benthic_PP_at_TIDE)
Spivak, AmandaWoods Hole Oceanographic Institution (WHOI)Principal Investigator
Luk, SheronWoods Hole Oceanographic Institution (WHOI)Contact
Rauch, ShannonWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager

Bulk soil and elemental properties of marsh and infilled pond soils within Plum Island Ecosystems - LTER. Data were collected 2014-2015.


Spatial Extent: N:42.741354 E:-70.8300014 S:42.7373999 W:-70.8472003
Temporal Extent: 2014-07 - 2015-12

Dataset Description

Bulk soil and elemental properties of marsh and infilled pond soils within Plum Island Ecosystems - LTER. Bulk soil properties of the soils are complemented with Fourier Transform Infrared Spectroscopy and ramped pyrolysis oxidation measurements of the soils. Data were collected during 2014-2015.

Methods & Sampling

Soil cores were collected from three sites within the Plum Island Ecosystems - Long Term Ecological Research (PIE-LTER) domain (MA, USA; 42.74° N, -70.85° W). A core liner was fitted with a gasketed piston and placed on the sediment surface and pushed down into the marsh subsurface, ensuring that the soil column did not compact during collection.

The sites had similar elevations (1.41 - 1.51 m North American Vertical Datum of 1988 [NAVD88]) and salt marsh grass communities, dominated by Spartina patens, S. alterniflora, and Distichlis spicata. Permanently inundated ponds within each site had comparable depths (0.24 - 0.30 m) but varied in size (643 - 7,149 m²) and age (40 - 53 years) (Spivak et al., 2017; Spivak et al., 2018).

Soil cores were split lengthwise and sectioned into 1, 2, or 5 cm sections, with higher resolution in the top 30 cm. Soil water content (%) and bulk density (g/cm³) were determined gravimetrically after drying to constant mass (60°C). Samples were sieved (1 mm) to remove root material and homogenized with a Retsch Mixer Mill 200. Soil samples were fumed with hydrochloric acid (Hedges and Stern, 1984) prior to elemental (total organic carbon [TOC]) and isotopic (δ13C) analyses at the Stable Isotope Laboratory in the Marine Biological Laboratory (Woods Hole, MA).

Data Processing Description

BCO-DMO Processing:
- replaced NaN and NA with nd as the missing data identifier;
- changed date format to YYYY-MM;
- renamed fields to conform with BCO-DMO naming conventions (no spaces or special characters).

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Data Files

(Comma Separated Values (.csv), 24.76 KB)
Primary data file for dataset ID 827298

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Related Publications

Spivak, A. C., Gosselin, K. M., & Sylva, S. P. (2018). Shallow ponds are biogeochemically distinct habitats in salt marsh ecosystems. Limnology and Oceanography. doi:10.1002/lno.10797
Spivak, A. C., Gosselin, K., Howard, E., Mariotti, G., Forbrich, I., Stanley, R., & Sylva, S. P. (2017). Shallow ponds are heterogeneous habitats within a temperate salt marsh ecosystem. Journal of Geophysical Research: Biogeosciences, 122(6), 1371–1384. doi:10.1002/2017jg003780 https://doi.org/10.1002/2017JG003780

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Site_IDSite number (1, 2, or 3); three high marsh sites were cored in PIE-LTER unitless
LocationLocation within each site (HIGH MARSH or POND); at each of the three sites, two high marsh cores and one inundated pond core were collected unitless
Core_IDCore identifier (1 or 2); the two marsh cores were labeled 1 and 2 unitless
DateMonth and year of sample collection; format: YYYY-MM unitless
LatLatitude of sampling site degrees North
LonLongitude of sampling site degrees East
Depth_minDepth minimum of sample horizon relative to surface of the marsh centimeters (cm)
Depth_maxDepth maximum of sample horizon relative to surface of the marsh centimeters (cm)
ElevationElevation of the soil horizon; North American Vertical Datum of 1988 meters (m)
Water_ContentWater content of soil by mass percent unitless (percent)
Dry_Bulk_DensityDry bulk density of soil by weight and volume of the soil horizon grams per cubic meter (g cm-3)
PorosityPorosity of soil unitless
TNPercent Total Nitrogen unitless (percent)
TOCPercent Total Organic Carbon unitless (percent)
C_NRatio of total organic carbon to total nitrogen unitless
Carbon_DensityTotal organic carbon accounting for dry bulk density of soil kilograms per cubic meter (kg m-3)
d13CThe stable isotopic carbon composition per mil

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Dataset-specific Instrument Name
Dual-Inlet and Continuous Flow Isotope Ratio Mass Spectrometer
Generic Instrument Name
Isotope-ratio Mass Spectrometer
Dataset-specific Description
Dual-Inlet and Continuous Flow Isotope Ratio Mass Spectrometer for elemental and stable isotopic analysis.
Generic Instrument Description
The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Dataset-specific Instrument Name
Retsch Mixer Mill 200
Generic Instrument Name
Dataset-specific Description
Retsch Mixer Mill 200 was used for homogenization.
Generic Instrument Description
A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.

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Project Information

Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary (Benthic_PP_at_TIDE)

Coverage: Plum Island Estuary, Rowley Massachusetts

Extracted from the NSF award abstract:
This project will address how rates of benthic microalgal production respond to eutrophication and geomorphological changes in human-impacted tidal creeks. Excess nutrient loading increases benthic algal biomass and likely stimulates production rates but the magnitude of nutrient and geomorphological effects on rates of production is unknown. Will changes in benthic algal productivity affect algal-bacterial coupling? Furthermore, how is algal-bacterial coupling affected by geomorphological changes, which may be exacerbated by excess nutrient loading but can also occur in pristine marshes?

This project will take advantage of the infrastructure of the TIDE project, a long-term saltmarsh eutrophication experiment at the Plum Island Ecosystem - Long Term Ecological Research site in Northeastern Massachusetts. Specifically, the PIs will measure benthic metabolism and examine algal- bacterial coupling in fertilized and ambient nutrient tidal creeks in the first field season. The following field season, they will compare sediment metabolism and carbon dynamics on slumped tidal creek walls (i.e. areas where low marsh has collapsed into the tidal creek) to that on the bottom of tidal creeks. In both years, gross and net production will be determined using an innovative triple oxygen isotope technique and traditional dissolved oxygen and inorganic carbon flux measurements. Comparisons between these methods will be useful in informing studies of sediment metabolism. Lipid biomarkers will be used to characterize the sources of organic matter to creek sediments, and stable isotope analysis of bacterial specific biomarkers to identify the sources of organic carbon utilized by sediment bacteria. The biomarkers will reveal whether sediment bacteria use organic matter substrates, such as benthic microalgal carbon, selectively or in proportion to availability. Overall, results from the proposed study will provide important information about how sediment carbon dynamics in shallow tidal creeks respond to long term eutrophication. Furthermore, findings will enhance understanding of the role of tidal creeks in coastal biogeochemistry.

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Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

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