http://lod.bco-dmo.org/id/dataset/827587
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-10-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbon and nitrogen stable isotopes of Hawaiian corals collected from August to November 2015
2020-10-26
publication
2020-10-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-12-03
publication
https://doi.org/10.26008/1912/bco-dmo.827587.1
Andréa G. Grottoli
Ohio State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Grottoli, A. G. (2020) Carbon and nitrogen stable isotopes of Hawaiian corals collected from August to November 2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-10-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.827587.1 [access date]
Carbon and nitrogen stable isotopes of Hawaiian corals Dataset Description: Methods and Sampling: <p>A complete description of the collection and experimental methods is available in Price et al. (in review). In brief, corals were collected between 17 August and 13 November 2015 from six sites (Electric Beach, Hale'iwa, Hawai'i Institute of Marine Biology [HIMB], Magic Island, Sampan Channel, and Waimanalo) surrounding the island of O'ahu, Hawai'i, USA. Ramets of seven coral species (<em>Montipora capitata, Montipora patula, Pocillopora acuta, Pocillopora meandrina, Porites compressa, Porites evermanni</em>, and <em>Porites lobata</em>) were collected at a depth of 0.5 to 5 m. A 5 to 10 cm coral ramet (branch or mound) was removed underwater via hammer and chisel from visually healthy parent colonies separated by at least 5 m on the reef to minimize the possibility of selecting corals of the same genet.</p>
<p><strong>Sample Solutions and Standards</strong>: All solutions were made with MilliQ water (18 MΩ; Millipore, MA) and ultrapure reagents unless otherwise noted. All labware was pre-cleaned with 10% v/v HCl and MilliQ water.</p>
<p><strong>Sample Preparation</strong>: Methods for the processing and separating coral host tissue and algal endosymbionts tissues for isotopic analyses are described in Price et al. (2020). Briefly, a small subsample (approximately 4 to 6 cm²) of each collected coral ramet was removed via hammer and a sterile chisel, the bulk coral tissue removed from the skeleton by airbrushing, and the resulting slurry was homogenized. A 0.5 ml sub-sample was removed, dried into silver capsules, and acidified via fumigation with 1N HCl for whole coral isotopic analysis. The remaining slurry was separated into animal host and endosymbiotic algal fractions via centrifugation and filtering steps. Overall, this process resulted in a whole coral sample, a host tissue sample, and an isolated algal endosymbiont sample for each coral collected.</p>
<p><strong>Elemental Analyses</strong><em>: </em>All samples were combusted using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) at the University of California (UC) Davis Stable Isotope Facility. The carbon isotopic signature of the animal host (δ¹³C<sub>h</sub>), algal endosymbiont (δ¹³C<sub>e</sub>), and whole coral (δ¹³C<sub>w</sub>) are reported as the per mil deviation of the stable isotopes ¹³C:¹²C relative to Vienna Peedee Belemnite Limestone Standard (v-PDB). Repeated measures of internal standards had a standard deviation of ± 0.2‰ for δ¹³C. The nitrogen isotopic signature of the animal host (δ¹⁵N<sub>h</sub>), algal endosymbiont (δ¹⁵N<sub>e</sub>), and whole coral (δ¹⁵N<sub>w</sub>) are reported as the per mil deviation of the stable isotopes ¹⁵N:¹⁴N relative to air. Repeated measures of internal standard had a standard deviation of ± 0.2‰ for δ¹⁵N. At least 10% of all coral measurements were made in duplicate. The standard deviation of duplicate sample analyses was ± 0.14‰ for δ¹³C<sub>h</sub>, ± 0.26‰ for δ¹³C<sub>e</sub>, ± 0.12‰ for the δ¹³C<sub>w</sub>, ± 0.07‰ for δ¹⁵N<sub>h</sub>, ± 0.22‰ for δ¹⁵N<sub>e</sub>, and ± 0.06‰ for the δ¹⁵N<sub>w</sub>.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459536 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459536
completed
Andréa G. Grottoli
Ohio State University
614-292-5782
School of Earth Sciences 125 South Oval Mall
Columbus
OH
43210
United States
grottoli.1@osu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Location
Site
Lat
Long
Collection_Date
Species
Sample_ID
d13Ch
d15Nh
d13Ce
d15Ne
d13Ch_e
d15Nh_e
d13Cw
d15Nw
PDZ Europa ANCA-GSL elemental analyzer
PDZ Europa 20-20 isotope ratio mass spectrometer
theme
None, User defined
site
latitude
longitude
date
species scientific name (binomial)
sample identification
d13C measured in biota
d15N measured in biota
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Phenotype and genotype of coral adaptation and acclimatization to global change
https://osprey.bco-dmo.org/project/546273
Phenotype and genotype of coral adaptation and acclimatization to global change
<p><strong>Project Summary:</strong><br />
<em>Overview: </em>This study proposes to evaluate the adaptation and acclimatization capacity of eight species of Hawaiian corals to long-term exposure of elevated temperature and ocean acidification (OA) conditions using a two-part approach: 1) a survey of natural corals found across natural temperature and pCO2 gradients and 2) a two-year long mesocosm study which will expose corals collected in part 1 to a range of temperature and pCO2 conditions expected this century. In both approaches, the phenotypic (i.e., physiological and biogeochemical) responses of corals to future climate change will be measured in conjunction with the already funded genotypic (i.e., genomic and transcriptomic) responses of the same corals by Dr. Rob Toonen. This study will address variation at both the population and species level. It will also be the first study to examine the effects of elevated temperature and pCO2 on corals in replicated mesocosms over a multiannual timeframe with a comprehensive suite of physiological, biogeochemical, and genomic tools.</p>
<p><em>Intellectual Merit: </em>Coral reefs are among the most diverse ecosystems on the planet, housing an estimated 25% of marine species. Yet, they appear to be especially susceptible to the effects of climate change and ocean acidification. To date, the assumption has been that corals will not be able to adapt because the rates of anthropogenically driven ocean acidification and climate change are too high. But there is little experimental evidence to evaluate that assumption. Recent models highlight the critical importance of that assumption in determining coral extinction risk, and several recent studies (including a couple of recent ones from Grottoli’s group) indicate that previous studies may have underestimated the potential for corals to acclimatize or adapt to global change. Here, quantitative, empirical estimates of the potential for long-term coral acclimatization and adaptation under global change scenarios will be made. The proposed study includes ~97% of the corals in the Hawaiian archipelago, yielding extensive spatial and biological relevance for the study. Lastly, this research brings together the expertise of Grottoli at OSU (coral physiologist and biogeochemist), Toonen at UH (marine molecular biologist), and McCulloch at UWA (geochemist) in a unique collaboration that blends a large suite of genetic, physiological, and biogeochemical tools to build an unprecedentedly comprehensive picture of coral adaptation and acclimation to global change. Thus, this work has the potential to transform our conceptual and empirical understanding of how corals respond to rapid environmental change.</p>
<p><em>Broader Impacts:</em> Half of the species in the Hawaiian archipelago are endemic, making Hawaiian coral reefs a high priority for biodiversity conservation. Results from the proposed work will be used for adaptive management plans that collaborator Dr. Toonen is involved in with the goal of preserving Hawaiian coral biodiversity in a UNESCO World Heritage Site -- the Papahanaumokuakea Marine National Monument (PMNM). PMNM encompasses the northwestern Hawaiian Islands, is renowned as one of the most pristine and highly protected coral reefs remaining on the planet, is the single largest conservation area under the U.S. flag, and one of the largest marine conservation areas in the world. This project will provide a bridge between short-term, single-species studies and longer-term, multi-species responses to global change in reef community settings. Findings from this work will be communicated at scientific meetings, through peer-reviewed journal publications, and via press releases. Grottoli will also bring her research and enthusiasm for marine science into her classrooms and onto the podium when giving general audience and professional talks. She has an established track record of recruiting and promoting under-represented students and will continue to do so. This project will recruit 3 undergrads and 2 high school students for supported senior thesis/independent research and provide an environment that will foster their passion and skills necessary to pursue career options in STEM disciplines.</p>
Coral Adaptation
largerWorkCitation
project
eng; USA
oceans
-158.131564
-157.683056
21.286111
21.594193
2015-08-17
2015-11-13
Oahu, HI and Hawaii Institute of Marine Biology
0
BCO-DMO catalogue of parameters from Carbon and nitrogen stable isotopes of Hawaiian corals collected from August to November 2015
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/827644.rdf
Name: Location
Units: unitless
Description: <p>Location where samples were collected (State and Country)</p>
http://lod.bco-dmo.org/id/dataset-parameter/827645.rdf
Name: Site
Units: unitless
Description: <p>Name of site where samples were collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/827646.rdf
Name: Lat
Units: degrees North
Description: <p>Site latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/827647.rdf
Name: Long
Units: degrees East
Description: <p>Site longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/827648.rdf
Name: Collection_Date
Units: unitless
Description: <p>Sample collection date; format: YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/827649.rdf
Name: Species
Units: unitless
Description: <p>Name of coral species</p>
http://lod.bco-dmo.org/id/dataset-parameter/827650.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/827651.rdf
Name: d13Ch
Units: per mil (‰)
Description: <p>The carbon isotopic signature of the animal host reported as the per mil deviation of the stable isotopes 13C:12C relative to Vienna Peedee Belemnite Limestone Standard (v-PDB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/827652.rdf
Name: d15Nh
Units: per mil (‰)
Description: <p>The nitrogen isotopic signature of the animal host reported as the per mil deviation of the stable isotopes 15N:14N relative to air</p>
http://lod.bco-dmo.org/id/dataset-parameter/827653.rdf
Name: d13Ce
Units: per mil (‰)
Description: <p>The carbon isotopic signature of the algal endosymbiont reported as the per mil deviation of the stable isotopes 13C:12C relative to Vienna Peedee Belemnite Limestone Standard (v-PDB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/827654.rdf
Name: d15Ne
Units: per mil (‰)
Description: <p>The nitrogen isotopic signature of the algal endosymbiont reported as the per mil deviation of the stable isotopes 15N:14N relative to air</p>
http://lod.bco-dmo.org/id/dataset-parameter/827655.rdf
Name: d13Ch_e
Units: per mil (‰)
Description: <p>The difference of d13Ch and d13Ce</p>
http://lod.bco-dmo.org/id/dataset-parameter/827656.rdf
Name: d15Nh_e
Units: per mil (‰)
Description: <p>The difference of d15Nh and d15Ne</p>
http://lod.bco-dmo.org/id/dataset-parameter/827657.rdf
Name: d13Cw
Units: per mil (‰)
Description: <p>The carbon isotopic signature of the whole coral reported as the per mil deviation of the stable isotopes 13C:12C relative to Vienna Peedee Belemnite Limestone Standard (v-PDB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/827658.rdf
Name: d15Nw
Units: per mil (‰)
Description: <p>The nitrogen isotopic signature of the whole coral reported as the per mil deviation of the stable isotopes 15N:14N relative to air</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
53111
https://darchive.mblwhoilibrary.org/bitstream/1912/26437/1/dataset-827587_coral-stable-isotopes__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.827587.1
download
onLine
dataset
<p>A complete description of the collection and experimental methods is available in Price et al. (in review). In brief, corals were collected between 17 August and 13 November 2015 from six sites (Electric Beach, Hale'iwa, Hawai'i Institute of Marine Biology [HIMB], Magic Island, Sampan Channel, and Waimanalo) surrounding the island of O'ahu, Hawai'i, USA. Ramets of seven coral species (<em>Montipora capitata, Montipora patula, Pocillopora acuta, Pocillopora meandrina, Porites compressa, Porites evermanni</em>, and <em>Porites lobata</em>) were collected at a depth of 0.5 to 5 m. A 5 to 10 cm coral ramet (branch or mound) was removed underwater via hammer and chisel from visually healthy parent colonies separated by at least 5 m on the reef to minimize the possibility of selecting corals of the same genet.</p>
<p><strong>Sample Solutions and Standards</strong>: All solutions were made with MilliQ water (18 MΩ; Millipore, MA) and ultrapure reagents unless otherwise noted. All labware was pre-cleaned with 10% v/v HCl and MilliQ water.</p>
<p><strong>Sample Preparation</strong>: Methods for the processing and separating coral host tissue and algal endosymbionts tissues for isotopic analyses are described in Price et al. (2020). Briefly, a small subsample (approximately 4 to 6 cm²) of each collected coral ramet was removed via hammer and a sterile chisel, the bulk coral tissue removed from the skeleton by airbrushing, and the resulting slurry was homogenized. A 0.5 ml sub-sample was removed, dried into silver capsules, and acidified via fumigation with 1N HCl for whole coral isotopic analysis. The remaining slurry was separated into animal host and endosymbiotic algal fractions via centrifugation and filtering steps. Overall, this process resulted in a whole coral sample, a host tissue sample, and an isolated algal endosymbiont sample for each coral collected.</p>
<p><strong>Elemental Analyses</strong><em>: </em>All samples were combusted using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) at the University of California (UC) Davis Stable Isotope Facility. The carbon isotopic signature of the animal host (δ¹³C<sub>h</sub>), algal endosymbiont (δ¹³C<sub>e</sub>), and whole coral (δ¹³C<sub>w</sub>) are reported as the per mil deviation of the stable isotopes ¹³C:¹²C relative to Vienna Peedee Belemnite Limestone Standard (v-PDB). Repeated measures of internal standards had a standard deviation of ± 0.2‰ for δ¹³C. The nitrogen isotopic signature of the animal host (δ¹⁵N<sub>h</sub>), algal endosymbiont (δ¹⁵N<sub>e</sub>), and whole coral (δ¹⁵N<sub>w</sub>) are reported as the per mil deviation of the stable isotopes ¹⁵N:¹⁴N relative to air. Repeated measures of internal standard had a standard deviation of ± 0.2‰ for δ¹⁵N. At least 10% of all coral measurements were made in duplicate. The standard deviation of duplicate sample analyses was ± 0.14‰ for δ¹³C<sub>h</sub>, ± 0.26‰ for δ¹³C<sub>e</sub>, ± 0.12‰ for the δ¹³C<sub>w</sub>, ± 0.07‰ for δ¹⁵N<sub>h</sub>, ± 0.22‰ for δ¹⁵N<sub>e</sub>, and ± 0.06‰ for the δ¹⁵N<sub>w</sub>.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PDZ Europa ANCA-GSL elemental analyzer
PDZ Europa ANCA-GSL elemental analyzer
PI Supplied Instrument Name: PDZ Europa ANCA-GSL elemental analyzer PI Supplied Instrument Description:All samples were combusted using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) at the University of California (UC) Davis Stable Isotope Facility Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
PDZ Europa 20-20 isotope ratio mass spectrometer
PDZ Europa 20-20 isotope ratio mass spectrometer
PI Supplied Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer PI Supplied Instrument Description:All samples were combusted using a PDZ Europa ANCA-GSL elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) at the University of California (UC) Davis Stable Isotope Facility. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/