http://lod.bco-dmo.org/id/dataset/827826
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2020-10-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Microbial cell counts in seawater samples collected on R/V Nerissa and R/V Yellowfin cruises at the San Pedro Ocean Time-series (SPOT) in 2017
2020-10-28
publication
2020-10-28
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-01-04
publication
https://doi.org/10.26008/1912/bco-dmo.827826.1
Sergio A. Sanudo-Wilhelmy
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sanudo-Wilhelmy, S. A. (2021) Microbial cell counts in seawater samples collected on R/V Nerissa and R/V Yellowfin cruises at the San Pedro Ocean Time-series (SPOT) in 2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-10-28 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.827826.1 [access date]
Microbial cell counts in seawater samples from SPOT cruises in 2017 Dataset Description: Methods and Sampling: <p>Samples for microbial cell counts were collected at&nbsp;six depths within the euphotic zone (5-250 m). Seawater was collected from each CTD depth using Niskin bottles, immediately fixed with formalin 2% (0.72% formaldehyde final concentration), and immediately frozen at -80C. Autotrophic picoplankton (Prochlorococcus, Synechococcus, and picoeukaryotes) and heterotrophic prokaryotes were enumerated by flow cytometry (Becton Dickinson FACSCalibur) (Gasol and del Giorgio,&nbsp;2000).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1559276 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1559276
completed
Sergio A. Sanudo-Wilhelmy
University of Southern California
213-821-1302
3616 Trousdale Pkwy Dept. of Biological Sciences, AHF 206, Mail Code: 0371
Los Angeles
CA
90089
USA
sanudo@usc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise
Date
Time_Start
Time_End
Longitude
Latitude
Depth
HNA_bacteria
LNA_bacteria
Total_Bacteria
Pro
Syn
Syn_HighF
Syn_total
Pico_small
Pico_large
Pico_total
Nano
Becton Dickinson FACSCalibur
Niskin bottles
theme
None, User defined
cruise name
date
time of day
longitude
latitude
depth
abundance
featureType
BCO-DMO Standard Parameters
Flow Cytometer
Niskin bottle
instrument
BCO-DMO Standard Instruments
SPOT_Nerissa_Cruises_2017
SPOT_Yellowfin_Cruises
service
Deployment Activity
San Pedro Ocean Time Series
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The role of organic and metal cofactors on the biogenic synthesis of halogenated volatile hydrocarbons
https://osprey.bco-dmo.org/project/712782
The role of organic and metal cofactors on the biogenic synthesis of halogenated volatile hydrocarbons
<p><em>NSF Award Abstract:</em><br />
Volatile halogenated hydrocarbon gases, in this case halomethanes, are produced naturally by organisms in the ocean; which then serves as a source of these biogenic gases to the atmosphere. Their chemical reactions in the atmosphere are very similar to those of anthropogenic chlorofluorocarbons (CFCs). While CFCs are well-studied because they consume the ozone in the upper atmosphere that shields the earth from harmful ultraviolet radiation, halomethanes have been largely neglected, even though they currently account for 25% of the ozone depletion. As anthropogenic CFC levels steadily decline, however, halomethanes are predicted to account for 50% of ozone depletion by 2050. Based on limited study thus far, marine halomethane production has been ascribed mainly to phytoplankton and macro algae. This project will build on new and compelling data that suggests marine heterotrophic bacteria could also be major producers of halomethanes. The data produced here will provide the critical evaluation required to address discrepancies in global halomethane budgets which currently are out of balance due to an unknown source to the atmosphere, evaluating the hypothesis that marine heterotrophic bacteria can supply this missing source. Concerns over the stability of the earth's stratospheric ozone layer make this valuable and necessary research with added value of providing support for engaged undergraduate, graduate, and postdoctoral education at the University of Southern California.</p>
<p>Past research on the production of marine halomethanes has focused on phytoplankton and macro algae, while potential bacterial contributions to the processe have been neglected. This research proposes to study the role of marine heterotrophic bacteria on the production of halomethanes. It has been noted in past studies that there are discrepancies in the global atmospheric halomethane budget, and it is possible this is due to a large missing bacterial source. Additionally, this research will evaluate the potential importance of vitamin B12, methionine, and vanadium cofactors on the synthesis of halomethanes in bacteria. A large portion of marine bacteria cannot synthesize methylation co-enzymes, and therefore, would require available B12, methionine, and vanadium from external sources to complete the methylation step. This study will also measure concentrations of halomethanes, B12, methionine, and vanadium in upwelling regions as well as at a long-term time series site in order to put constraints on the variability of halomethanes concentrations for use in global linked air-sea models.</p>
Volatile_Hydrocarbons
largerWorkCitation
project
eng; USA
oceans
San Pedro Ocean Time Series
-118.4
-118.4
33.55
33.55
2017-03-15
2017-12-15
0
BCO-DMO catalogue of parameters from Microbial cell counts in seawater samples collected on R/V Nerissa and R/V Yellowfin cruises at the San Pedro Ocean Time-series (SPOT) in 2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/827876.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise name, month, and year</p>
http://lod.bco-dmo.org/id/dataset-parameter/827877.rdf
Name: Date
Units: unitless
Description: <p>Date; format: YYYY-MM-DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/827878.rdf
Name: Time_Start
Units: unitless
Description: <p>Start time; format: hh:mm</p>
http://lod.bco-dmo.org/id/dataset-parameter/827879.rdf
Name: Time_End
Units: unitless
Description: <p>End time; format: hh:mm</p>
http://lod.bco-dmo.org/id/dataset-parameter/827880.rdf
Name: Longitude
Units: degrees East
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/827881.rdf
Name: Latitude
Units: degrees North
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/827882.rdf
Name: Depth
Units: meters (m)
Description: <p>Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/827883.rdf
Name: HNA_bacteria
Units: cells per milliter (cell ml-1)
Description: <p>Number of high nucleic acid content bacterial cells per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827884.rdf
Name: LNA_bacteria
Units: cells per milliter (cell ml-1)
Description: <p>Number of low nucleic acid content bacterial cells per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827885.rdf
Name: Total_Bacteria
Units: cells per milliter (cell ml-1)
Description: <p>Number of total bacteria (high + low nucleic acid content) per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827886.rdf
Name: Pro
Units: cells per milliter (cell ml-1)
Description: <p>Number of Prochlorococcus cells per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827887.rdf
Name: Syn
Units: cells per milliter (cell ml-1)
Description: <p>Number of Synechococcus cells (regular fluorescence) per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827888.rdf
Name: Syn_HighF
Units: picomoles per liter (pico mol L-1)
Description: <p>Number of Synechococcus cells (high fluorescence) per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827889.rdf
Name: Syn_total
Units: picomoles per liter (pico mol L-1)
Description: <p>Number of Synechococcus cells (regular fluorescence + high fluorescence) per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827890.rdf
Name: Pico_small
Units: picomoles per liter (pico mol L-1)
Description: <p>Number of small picoeukaryotic cells per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827891.rdf
Name: Pico_large
Units: picomoles per liter (pico mol L-1)
Description: <p>Number of large picoeukaryotic cells per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827892.rdf
Name: Pico_total
Units: picomoles per liter (pico mol L-1)
Description: <p>Number of total picoeukaryotic cells (small + large) per milliliter of seawater measured with Flow Cytometry</p>
http://lod.bco-dmo.org/id/dataset-parameter/827893.rdf
Name: Nano
Units: picomoles per liter (pico mol L-1)
Description: <p>Number of total nanophytoplanktonic cells per milliliter of seawater measured with Flow Cytometry</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
5413
https://darchive.mblwhoilibrary.org/bitstream/1912/26525/1/dataset-827826_spot-cell-counts__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.827826.1
download
onLine
dataset
<p>Samples for microbial cell counts were collected at&nbsp;six depths within the euphotic zone (5-250 m). Seawater was collected from each CTD depth using Niskin bottles, immediately fixed with formalin 2% (0.72% formaldehyde final concentration), and immediately frozen at -80C. Autotrophic picoplankton (Prochlorococcus, Synechococcus, and picoeukaryotes) and heterotrophic prokaryotes were enumerated by flow cytometry (Becton Dickinson FACSCalibur) (Gasol and del Giorgio,&nbsp;2000).</p>
Specified by the Principal Investigator(s)
<p>The flow cytometry data were analyzed and processed using BD FACStation software from Becton Dickinson.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Becton Dickinson FACSCalibur
Becton Dickinson FACSCalibur
PI Supplied Instrument Name: Becton Dickinson FACSCalibur PI Supplied Instrument Description:Autotrophic picoplankton (Prochlorococcus, Synechococcus, and picoeukaryotes) and heterotrophic prokaryotes were enumerated by flow cytometry (Becton Dickinson FACSCalibur). Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles PI Supplied Instrument Description:Seawater was collected from each CTD depth using Niskin bottles. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: SPOT_Nerissa_Cruises_2017
SPOT_Nerissa_Cruises_2017
R/V Nerissa
vessel
Cruise: SPOT_Yellowfin_Cruises
SPOT_Yellowfin_Cruises
R/V Yellowfin
R/V Yellowfin
vessel
R/V Nerissa
vessel
R/V Yellowfin
R/V Yellowfin
vessel